Comparative evaluation of fibre optic immunosensor with ELISA and IFAT for serodiagnosis of Indian Kala azar

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1997-01-01 DOI:10.1016/S0888-0786(96)01080-3

Nidhi Nath, Seema R. Jain, Sneh Anand

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Abstract

Fibre optic immunosensor for the detection of the antibodies specific for Leishmania donovani in the sera of patients suffering from visceral leishmaniasis has been designed and fabricated. Technique invovles a two-step sandwich-type assay that gives quick analysis and is easy to use. Purified cell surface protein of L. donovani had been immobilized on tapered fibre optic core by covalent coupling. In the first step, treated fibres were incubated with the patient serum for 10 min and then with FITC-tagged goat anti-human IgG in the second step. FITC provided the specific fluorescent signal for detection. No accurate volumetric measurement of serum sample is desirable because of test fibre design and the whole procedure takes just 15 min. Fluorescent signal has been found to be sensitive and specific for Visceral leishmaniasis and correlates well with the conventional serodiagnostic procedures, like ELISA and IFAT.

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光纤免疫传感器与ELISA和IFAT诊断印度黑热病的比较评价

设计并制作了用于检测内脏利什曼病患者血清中多诺瓦利什曼原虫特异性抗体的光纤免疫传感器。该技术包括两步三明治式分析，可快速分析且易于使用。用共价偶联法将纯化的多诺瓦菌细胞表面蛋白固定在锥形光纤芯上。在第一步，处理过的纤维与患者血清孵育10分钟，然后在第二步与fitc标记的山羊抗人IgG孵育。FITC为检测提供了特定的荧光信号。由于测试纤维的设计，不需要对血清样本进行精确的体积测量，而且整个过程只需15分钟。荧光信号对内脏利什曼病非常敏感和特异性，并且与ELISA和IFAT等传统血清诊断程序具有良好的相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Serodiagnosis and Immunotherapy in Infectious Disease

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