Serodiagnosis and Immunotherapy in Infectious Disease最新文献_第10页

Serodiagnosis & immunotherapy in infectious disease calendar 传染病日历中的血清诊断与免疫治疗

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1995-03-01 DOI: 10.1016/0888-0786(95)90004-7

引用次数: 0

Utility of lipopolysaccharides from Bordetella pertussis and Bordetella parapertussis in the serodiagnosis of pertussis and parapertussis 百日咳和副百日咳杆菌脂多糖在百日咳和副百日咳血清诊断中的应用

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1995-03-01 DOI: 10.1016/0888-0786(95)93023-5

K. Amano , T. Aoyama

{"title":"Utility of lipopolysaccharides from Bordetella pertussis and Bordetella parapertussis in the serodiagnosis of pertussis and parapertussis","authors":"K. Amano , T. Aoyama","doi":"10.1016/0888-0786(95)93023-5","DOIUrl":"10.1016/0888-0786(95)93023-5","url":null,"abstract":"<div><p><em>Bordetella pertussis</em> (Bp) and <em>Bordetella parapertussis</em> (Bpp), which are responsible for outbreaks of whooping cough in humans, are closely related, and it is difficult to discriminate between these species immunologically. We developed an immunodiagnostic method using an enzyme-linked immunosorbent assay (ELISA) or immunoblotting, on the basis of the serological differences between lipopolysaccharide (LPS) from Bp and Bpp. In ELISA, the sera from nine out of 11 patients with pertussis (about 80%) possessed high reactivities against whole cells (WC) of Bp in comparison with Bpp-WC, whereas the sera from five patients with the same disease (about 45%) reacted with Bp-LPS more than with Bpp-LPS. High reactivity against Bpp-WC and Bpp-LPS in the sera from 13 out of 16 patients with parapertussis (about 80%) was shown as compared with that against Bp-WC and Bp-LPS, respectively. Immunoblotting showed that all of the sera from pertussis patients reacted more strongly with Bp-LPS than with Bpp-LPS, except those which were unreactive. Almost all of the sera from parapertussis patients reacted with Bpp-LPS more than with Bp-LPS. These results indicated that immunoblotting, rather than ELISA, using LPS from Bp and Bpp is useful for serodiagnosis to distinguish between pertussis and parapertussis.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"7 1","pages":"Pages 7-14"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0888-0786(95)93023-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76795679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnosis of Neisseria gonorrhoeae and Chiamydia trachomatis infection using first-voided urine in men with urethritis 用男性尿道炎患者首次排尿诊断淋病奈瑟菌和沙眼衣原体感染

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1995-03-01 DOI: 10.1016/0888-0786(95)93024-6

A. Turner , I.D. Paul , J.T. Arumainayagam , T. Crowley , E.O. Caul

{"title":"Diagnosis of Neisseria gonorrhoeae and Chiamydia trachomatis infection using first-voided urine in men with urethritis","authors":"A. Turner , I.D. Paul , J.T. Arumainayagam , T. Crowley , E.O. Caul","doi":"10.1016/0888-0786(95)93024-6","DOIUrl":"10.1016/0888-0786(95)93024-6","url":null,"abstract":"<div><p>A study was designed to examine the feasibility of making the specific diagnoses of gonorrhoea and chlamydia infection from a single specimen of urine. Urine specimens and urethral swabs were collected from 212 male attenders at a genitourinary medicine clinic who had evidence of urethritis. Urethral swabs and urine sediments were cultured for <em>Neisseria gonorrhoeae</em> and urine sediments were also tested by Gonozyme and IDEIA enzyme immunoassays for <em>N. gonorrhoeae</em> and <em>Chlamydia trachomatis</em> antigens respectively Sixty-three urethral cultures were positive for <em>N. gonorrhoeae</em> and 83 tests were positive in the routine amplified immunoassay for chlamydia antigen. Nineteen patients had a dual infection of gonorrhoea and chlamydia. The incidence of chlamydia in non-gonococcal urethritis was 43%, this closely agreed with our previous studies. Urine deposit and urethral swab culture for <em>N. gonorrhoeae</em> gave a concordant negative result in 148 patients and urine culture detected 61 out of 63 patients found positive by urethral swab culture, a sensitivity of 96.8%. The immunoassay for gonococcal antigen in urine detected 60 out of 63 urethral culture positive patients. If two persistently equivocal results were taken as reactive then the sensitivity of the test was 98.4% with a specificity of 94%. Our results showed that both <em>N. gonorrhoeae</em> and <em>C. trachomatis</em> can be detected readily using appropriate enzyme immunoassays on a single urine sample from symptomatic males. This approach to sexually-transmitted disease (STD) screening may be applicable to mass populations.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"7 1","pages":"Pages 15-18"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0888-0786(95)93024-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89833727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serological response of patients with clinical typhoid 临床伤寒患者的血清学反应

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1995-03-01 DOI: 10.1016/0888-0786(95)93028-A

H. Chart, L.R. Ward, B. Rowe

引用次数: 10

Detection of verotoxin-producing Escherichia coli in stool specimens by the polymerase chain reaction 聚合酶链反应检测粪便标本中产维罗毒素大肠杆菌

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1994-12-01 DOI: 10.1016/0888-0786(94)90030-2

FTH Chan , N Stewart , F Diaz-Mitoma , PN McLaine

引用次数: 1

Detection of human immunodeficiency virus type 1 by a highly sensitive and specific polymerase chain reaction method 用高灵敏度和特异性的聚合酶链反应方法检测人类免疫缺陷病毒1型

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1994-12-01 DOI: 10.1016/0888-0786(94)90036-1

FY-T Tung

引用次数: 2

Quantitation of human immunodeficiency virus plasma RNA by branched DNA and reverse transcription coupled polymerase chain reaction assay methods: A critical evaluation of accuracy and reproducibility 通过支链DNA和逆转录偶联聚合酶链反应测定方法定量人类免疫缺陷病毒血浆RNA:准确性和可重复性的关键评估

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1994-12-01 DOI: 10.1016/0888-0786(94)90039-6

J Todd, T Yeghiazarian, B Hoo, J Detmer, J Kolberg, R White, J Wilber, M Urdea

{"title":"Quantitation of human immunodeficiency virus plasma RNA by branched DNA and reverse transcription coupled polymerase chain reaction assay methods: A critical evaluation of accuracy and reproducibility","authors":"J Todd, T Yeghiazarian, B Hoo, J Detmer, J Kolberg, R White, J Wilber, M Urdea","doi":"10.1016/0888-0786(94)90039-6","DOIUrl":"10.1016/0888-0786(94)90039-6","url":null,"abstract":"<div><p>The present study was designed to evaluate the utility of two assays, reverse transcription coupled polymerase chain reaction (RT-PCR) and branched DNA (bDNA), to accurately and reproducibly quantitate plasma human immunodeficiency virus (HIV) RNA levels. The bDNA assay quantitated RNA transcripts, prepared from different HIV-1 subtypes (A-E), within 1.5-fold. Similarly, the bDNA assay, standardized to subtype B, was used to quantitate cultured isolates from subtypes A, C-F within 2-fold; however, the RT-PCR assay displayed a 904-fold range. Reproducibility studies demonstrated that the bDNA and RT-PCR assays could be used statistically (<em>P</em><0.05) to discern less than 2- and 6.8 – 8.1-fold changes in RNA levels, respectively. This study showed that the two assay methods differ in accuracy and reproducibility. These differences need to be considered when choosing specific applications for the methods.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"6 4","pages":"Pages 233-239"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0888-0786(94)90039-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91467007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

The diagnostic value of PCR for the detection of enteroviral infections PCR检测肠道病毒感染的诊断价值

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1994-12-01 DOI: 10.1016/0888-0786(94)90031-0

N de Leeuw , WJG Melchers , DFM Willemse , AHMM Balk , N de Jonge , JMD Galama

引用次数: 4

Usefulness of polymerase chain reaction for detection of Bacteroides fragilis in clinical specimens 聚合酶链反应在临床脆弱拟杆菌检测中的应用

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1994-12-01 DOI: 10.1016/0888-0786(94)90042-6

H Koga, Y Yamashita, S Kohno, M Kaku, K Hara

引用次数: 0

Relative quantitation of HIV-1 proviral DNA amplified using the polymerase chain reaction 用聚合酶链反应扩增HIV-1前病毒DNA的相对定量

Serodiagnosis and Immunotherapy in Infectious Disease Pub Date : 1994-12-01 DOI: 10.1016/0888-0786(94)90034-5

AAM Jiman-Fatani, PJ Vallely, DJ Morris

引用次数: 0