{"title":"接种A + C组脑膜炎奈瑟菌和b型流感嗜血杆菌后血清抑菌和杀菌活性:三种功能测定的比较","authors":"S. Hugosson , H. Fredlund , H. Käyhty , P. Olcén","doi":"10.1016/S0888-0786(96)01082-7","DOIUrl":null,"url":null,"abstract":"<div><p>Three functional assays on immunity, serum bactericidal activity (SBA), phagocytic killing (PK) and an index calculation of chemiluminescence induced in polymorphonuclear leukocytes (CL-index), were compared with an enzyme linked immunoassay (ELISA) of the concentration of the total Ig-fraction of anticapsular antibodies in 28 healthy adults immunized against <em>Haemophilus influenzae</em> type b (Hib) or <em>Neisseria meningitidis</em> serogroup A and C (Me A + C). The immunization evoked a serum response measurable with all assays and a high concentration of antibodies against McA, McC and Hib usually was associated with functional activity and vice versa. However, qualitative differences were found and five of the vaccines (18%) were non-responders in functional assays despite a significant seroconversion measured by ELISA. A functional test may prove valuable in bacterial vaccine trials as complementary to immunochemical calculations of anticapsular antibodies. For estimates of opsonic activity bactericidal serum activity interferes in the PK assay, which is demonstrated in the present study. As an alternative, the CL assay, with the index technique described, is a reliable, less laborious and effective test on opsonophagocytosis.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"8 3","pages":"Pages 213-220"},"PeriodicalIF":0.0000,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0888-0786(96)01082-7","citationCount":"0","resultStr":"{\"title\":\"Serum opsonic and bactericidal activity after Neisseria meningitidis group A + C and Haemophilus influenzae type b vaccination: a comparison of three functional assays\",\"authors\":\"S. Hugosson , H. Fredlund , H. Käyhty , P. Olcén\",\"doi\":\"10.1016/S0888-0786(96)01082-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Three functional assays on immunity, serum bactericidal activity (SBA), phagocytic killing (PK) and an index calculation of chemiluminescence induced in polymorphonuclear leukocytes (CL-index), were compared with an enzyme linked immunoassay (ELISA) of the concentration of the total Ig-fraction of anticapsular antibodies in 28 healthy adults immunized against <em>Haemophilus influenzae</em> type b (Hib) or <em>Neisseria meningitidis</em> serogroup A and C (Me A + C). The immunization evoked a serum response measurable with all assays and a high concentration of antibodies against McA, McC and Hib usually was associated with functional activity and vice versa. However, qualitative differences were found and five of the vaccines (18%) were non-responders in functional assays despite a significant seroconversion measured by ELISA. A functional test may prove valuable in bacterial vaccine trials as complementary to immunochemical calculations of anticapsular antibodies. For estimates of opsonic activity bactericidal serum activity interferes in the PK assay, which is demonstrated in the present study. As an alternative, the CL assay, with the index technique described, is a reliable, less laborious and effective test on opsonophagocytosis.</p></div>\",\"PeriodicalId\":101161,\"journal\":{\"name\":\"Serodiagnosis and Immunotherapy in Infectious Disease\",\"volume\":\"8 3\",\"pages\":\"Pages 213-220\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0888-0786(96)01082-7\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Serodiagnosis and Immunotherapy in Infectious Disease\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0888078696010827\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Serodiagnosis and Immunotherapy in Infectious Disease","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0888078696010827","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
免疫、血清杀菌活性(SBA)、吞噬杀伤(PK) 3项功能测定和多形核白细胞诱导化学发光指数(CL-index)计算;与酶联免疫分析法(ELISA)比较了28名健康成人免疫b型流感嗜血杆菌(Hib)或脑膜炎奈瑟菌血清组A和C (Me A + C)的抗囊抗体总igg部分浓度。免疫引起了所有检测都可测量的血清反应,抗McA、McC和Hib的高浓度抗体通常与功能活性相关,反之亦然。然而,发现了质性差异,尽管ELISA测量了显著的血清转化,但在功能分析中,五种疫苗(18%)无应答。在细菌疫苗试验中,作为抗囊抗体免疫化学计算的补充,功能测试可能证明是有价值的。对于opsonic活性的估计,杀菌血清活性会干扰PK测定,这在本研究中得到了证明。作为一种替代方法,采用所描述的指数技术的CL测定法是一种可靠、不费力和有效的调理吞噬试验。
Serum opsonic and bactericidal activity after Neisseria meningitidis group A + C and Haemophilus influenzae type b vaccination: a comparison of three functional assays
Three functional assays on immunity, serum bactericidal activity (SBA), phagocytic killing (PK) and an index calculation of chemiluminescence induced in polymorphonuclear leukocytes (CL-index), were compared with an enzyme linked immunoassay (ELISA) of the concentration of the total Ig-fraction of anticapsular antibodies in 28 healthy adults immunized against Haemophilus influenzae type b (Hib) or Neisseria meningitidis serogroup A and C (Me A + C). The immunization evoked a serum response measurable with all assays and a high concentration of antibodies against McA, McC and Hib usually was associated with functional activity and vice versa. However, qualitative differences were found and five of the vaccines (18%) were non-responders in functional assays despite a significant seroconversion measured by ELISA. A functional test may prove valuable in bacterial vaccine trials as complementary to immunochemical calculations of anticapsular antibodies. For estimates of opsonic activity bactericidal serum activity interferes in the PK assay, which is demonstrated in the present study. As an alternative, the CL assay, with the index technique described, is a reliable, less laborious and effective test on opsonophagocytosis.