Revista del Laboratorio Clínico最新文献_第9页

Falso positivo a morfina en orina por reactividad cruzada con alimemazina 尿液中吗啡与alimemazine交叉反应假阳性

Revista del Laboratorio Clínico Pub Date : 2018-07-01 DOI: 10.1016/j.labcli.2018.02.002

Marta Ortega López-Peláez , Silvia Buendía Martínez , Marina Bravo Álvarez , Julia Asensio Antón , Joaquín Otero de Becerra , María Antonia Martínez González

引用次数: 1

Desarrollo de procedimientos de medida basados en la cromatografía líquida de alta resolución 基于高分辨率液相色谱法的测量程序的发展

Revista del Laboratorio Clínico Pub Date : 2018-07-01 DOI: 10.1016/j.labcli.2017.05.001

Raúl Rigo Bonnin, Francesca Canalias Reverter, Sara Esteve Poblador, Francisco Javier Gella Tomás, Bernardino González de la Presa, Rosa M. López Martínez

引用次数: 0

Enfermedad celíaca y sensibilidad al gluten no celíaca: la necesidad de uso de herramientas diagnósticas alternativas. Provocación controlada y nuevos marcadores 乳糜泻和非乳糜泻麸质敏感性:需要使用替代诊断工具。控制挑衅和新的标记

Revista del Laboratorio Clínico Pub Date : 2018-07-01 DOI: 10.1016/j.labcli.2017.10.003

José Antonio Garrote , Ángel San Miguel , Jesús Pachón , Rosario Pastor , Emilio Rodríguez Barbero

引用次数: 0

La Asociación Española de Biopatología Médica-Medicina del Laboratorio aclara conceptos sobre recertificación y registro profesionales 西班牙医学生物病理学-实验室医学协会澄清了重新认证和专业注册的概念

Revista del Laboratorio Clínico Pub Date : 2018-07-01 DOI: 10.1016/j.labcli.2017.11.005

Santiago Prieto Menchero , María Carmen Lorenzo Lozano , María Concepción Alonso-Cerezo

引用次数: 0

Reference values for serum folate and vitamin B12 in a Spanish population using an electrochemiluminiscent method 用电化学发光法测定西班牙人群血清叶酸和维生素B12的参考值

Revista del Laboratorio Clínico Pub Date : 2018-07-01 DOI: 10.1016/j.labcli.2017.11.002

José Luis Martín Calderón , Luis Caballero , Fernando Solano , Fernando Bustos

{"title":"Reference values for serum folate and vitamin B12 in a Spanish population using an electrochemiluminiscent method","authors":"José Luis Martín Calderón , Luis Caballero , Fernando Solano , Fernando Bustos","doi":"10.1016/j.labcli.2017.11.002","DOIUrl":"10.1016/j.labcli.2017.11.002","url":null,"abstract":"<div><h3>Background</h3><p>Despite being the most widely used medical decision-making tool, reference intervals are not usually determined by clinical laboratories, due to the highly demanding activities and costly process it involves. However, scientific societies encourage individual clinical laboratories to establish their own reference values. This is especially important in the cases of folate and vitamin B<sub>12</sub>, due to strong differences in vitamin status among different populations.</p></div><div><h3>Objective</h3><p>Our aim is to establish reference intervals for folate and vitamin B<sub>12</sub> levels in a healthy blood donor population using an electrochemiluminiscent method (ROCHE DIAGNOSTICS).</p></div><div><h3>Method</h3><p>Folate and vitamin B<sub>12</sub> levels were measured in 141 healthy blood donors aged between 18 and 65 years. Biochemical analyses were performed using a Modular E170 analyzer (ROCHE DIAGNOSTICS) and an electrochemiluminiscent method. Reference intervals were calculated with a non-parametric percentile method following the CLSI guidelines.</p></div><div><h3>Results</h3><p>There were not significant differences in folate or cobalamin levels between age or sex subgroups. The limits of the reference interval for folate were 2.2 and 18ng/mL (5–40.7nmol/L), and 213.8 and 763.3pg/mL (158.2–564.8pmol/L) for vitamin B<sub>12</sub>. These intervals differed from those claimed by the manufacturer.</p></div><div><h3>Conclusions</h3><p>Our results emphasize the convenience of building reference values based on the population served by the laboratory, in order to unequivocally rule out deficiencies of folate or vitamin B<sub>12.</sub></p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.11.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72864293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estabilidad de analitos conservados en tubo cerrado con film versus cerrado con tapa plástica 用薄膜密封管保存的分析物与用塑料盖密封的分析物的稳定性

Revista del Laboratorio Clínico Pub Date : 2018-07-01 DOI: 10.1016/j.labcli.2018.01.003

Juan Antonio Verna, Fernando Daniel Ventimiglia, Lorena Maydana, Ana Aristimuño, Jorge José Bruno, Liliana Elena D’Agostino

{"title":"Estabilidad de analitos conservados en tubo cerrado con film versus cerrado con tapa plástica","authors":"Juan Antonio Verna, Fernando Daniel Ventimiglia, Lorena Maydana, Ana Aristimuño, Jorge José Bruno, Liliana Elena D’Agostino","doi":"10.1016/j.labcli.2018.01.003","DOIUrl":"10.1016/j.labcli.2018.01.003","url":null,"abstract":"<div><h3>Introduction</h3><p>The laboratory must be able to guarantee the stability of stored patient samples for confirmation of results or errors in the interpretation of the medical request. The aim of this study was to evaluate the stability of the blood analytes preserved in tubes sealed with film versus a plastic cap.</p></div><div><h3>Material and methods</h3><p>A total of 24 blood samples were aliquoted into two tubes; one sealed with film, and the other with a plastic cap. Twenty-six clinical chemistry analytes were measured for 7 consecutive days using a Roche Cobas c 501 autoanalyser. The samples were stored in a refrigerator between 2-8C. The total error and coefficient of variation were calculated, and the results were compared against the quality requirements of the laboratory.</p></div><div><h3>Conclusions</h3><p>The variation was higher in the tubes sealed with film than in those with a plastic cap. Enzymes remained within the quality requirements for four days, and ions, apart from iron, were stable for only one day. The metabolites were stable for seven days excluding glucose, uric acid, total proteins, and albumin. It is suggested that each laboratory must evaluate the stability of analytes based on its workflow.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2018.01.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88742310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Análisis del líquido pleural: parámetros bioquímicos de uso infrecuente 胸膜液分析:不常用的生化参数

Revista del Laboratorio Clínico Pub Date : 2018-07-01 DOI: 10.1016/j.labcli.2017.10.001

María José Soler-Sempere , Marina Pellicer-Mas , José F. Sánchez-Hernández , Eduardo García-Pachón

引用次数: 2

El paradigma de la sepsis: en busca del biomarcador perfecto 败血症的范例:寻找完美的生物标志物

Revista del Laboratorio Clínico Pub Date : 2018-04-01 DOI: 10.1016/j.labcli.2017.07.001

Cristina Andrés Ledesma , María Dolores Calvo Nieves , David Andaluz-Ojeda

引用次数: 2

Influencia del sexo fetal en los marcadores de riesgo utilizados en el cribado prenatal de trisomía 21 胎儿性别对产前21三体筛查危险标志物的影响

Revista del Laboratorio Clínico Pub Date : 2018-04-01 DOI: 10.1016/j.labcli.2017.10.002

Ignacio Peral Camacho , Esperanza Lepe Balsalobre , José Antonio Sainz Bueno , Antonio Moro Ortiz

{"title":"Influencia del sexo fetal en los marcadores de riesgo utilizados en el cribado prenatal de trisomía 21","authors":"Ignacio Peral Camacho , Esperanza Lepe Balsalobre , José Antonio Sainz Bueno , Antonio Moro Ortiz","doi":"10.1016/j.labcli.2017.10.002","DOIUrl":"10.1016/j.labcli.2017.10.002","url":null,"abstract":"<div><h3>Introduction</h3><p>In first trimester combined screening, biochemical and ultrasound markers are used that are modified by different factors such as weight, twins, smoking, etc. The aim of this study is to analyse the influence of foetal gender on these markers, and the repercussion on the result of the screening of trisomy 21.</p></div><div><h3>Material and methods</h3><p>An observational, descriptive and retrospective study (2013-2015) was conducted. Multiple gestations were excluded. Variables analysed: nuchal translucency, free human chorionic gonadotrophin concentration, pregnancy-associated plasma protein A, and their median corrected multiples. A comparison was made of the medians of the variables in gestations with male and female foetuses, affected and non-affected.</p></div><div><h3>Results</h3><p>There was a 23.62% increase in the median of the median corrected multiple free human chorionic gonadotrophin and 3.65% in the median of the median corrected multiple pregnancy-associated plasma protein A, in gestations with female foetuses. This increase is observed in gestations with foetuses affected and non-affected by trisomy 21. Male gender: detection rate 86.9%, false positive rate 3.36%. Female gender: detection rate 90.9%, false positive rate 4.10%.</p></div><div><h3>Conclusions</h3><p>The increase, especially in free human chorionic gonadotrophin levels, leads to an increase in detection rate and false positive rate in gestations with a female foetus. The application of a correction factor by gender requires cost-effectiveness studies.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.10.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74109278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitorización terapéutica de metotrexato en pacientes leucémicos mediante cromatografía líquida de alta resolución. Desarrollo, validación y aplicación clínica del método analítico 用高分辨率液相色谱法监测甲氨蝶呤在白血病患者中的治疗性监测。分析方法的开发、验证和临床应用

Revista del Laboratorio Clínico Pub Date : 2018-04-01 DOI: 10.1016/j.labcli.2017.09.007

Lucía Torregiani, Yamile Soledad Caro, María Mercedes De Zan

{"title":"Monitorización terapéutica de metotrexato en pacientes leucémicos mediante cromatografía líquida de alta resolución. Desarrollo, validación y aplicación clínica del método analítico","authors":"Lucía Torregiani, Yamile Soledad Caro, María Mercedes De Zan","doi":"10.1016/j.labcli.2017.09.007","DOIUrl":"10.1016/j.labcli.2017.09.007","url":null,"abstract":"<div><h3>Introduction</h3><p>Therapeutic drug monitoring of methotrexate (MTX) is a practice used during acute lymphoblastic leukaemias treatments in order to adjust the leucovorin rescue dose and avoid toxicity. The aim of this work was to evaluate the performance of a bioanalytical method based on liquid chromatography in the control of paediatric acute lymphoblastic leukaemia treatments.</p></div><div><h3>Materials and methods</h3><p>MTX plasma concentrations were evaluated in a total of 61 patients, divided in 2 groups according to the treatment: 24h infusion and 36h infusion. The method used 250μL aliquots of pre-processed plasma, using a validated analytical method, in an Agilent 1260 chromatograph with a 5μm Zorbax Eclipse XDB-C18 column (4.6×150mm) and a mobile phase composed of acetonitrile and 0.05mol/L of a sodium acetate buffer solution at pH 3.5. The detection was performed at 305nm.</p></div><div><h3>Results</h3><p>The method did not show interferences by metabolites or other drugs. The lower limit of quantitation was 0.05μmol/L. Inter- and intra-assay precision and accuracy were acceptable in the working range (0.05-5.0μmol/L), and linear behaviour was observed. Toxic plasma levels of MTX were observed in 29.4% and 60% of the patients from 24h infusion and 36h infusion, respectively. Therefore, leucovorin rescue treatment was either applied earlier or reinforced.</p></div><div><h3>Conclusions</h3><p>The chromatography method was efficient and accessible for the therapeutic drug monitoring of MTX in paediatric patients, allowing appropriate medical decision making within a short time of analysis.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.09.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79936511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1