Revista del Laboratorio Clínico最新文献_第8页

Mecanismos inflamatorios y fibróticos en la enfermedad renal. Protagonistas y terapéutica 肾脏疾病的炎症和纤维化机制。主角和治疗

Revista del Laboratorio Clínico Pub Date : 2018-10-01 DOI: 10.1016/j.labcli.2017.11.004

Laura Salanova Villanueva, Begoña Santos Sánchez-Rey, Marta Sanz Sainz

引用次数: 0

Influencia del informe de laboratorio en el diagnóstico de la disfunción tiroidea en la gestante: más allá del asterisco 实验室报告对孕妇甲状腺功能障碍诊断的影响:星号以外

Revista del Laboratorio Clínico Pub Date : 2018-10-01 DOI: 10.1016/j.labcli.2018.03.001

Luis Javier Morales-García, María Pacheco-Delgado, Santiago Prieto Menchero, Daniel Pineda Tenor

{"title":"Influencia del informe de laboratorio en el diagnóstico de la disfunción tiroidea en la gestante: más allá del asterisco","authors":"Luis Javier Morales-García, María Pacheco-Delgado, Santiago Prieto Menchero, Daniel Pineda Tenor","doi":"10.1016/j.labcli.2018.03.001","DOIUrl":"10.1016/j.labcli.2018.03.001","url":null,"abstract":"<div><h3>Introduction</h3><p>Thyroid physiology undergoes adaptive changes during pregnancy, making it necessary to know the reference ranges of thyroid hormones according to gestational age for a correct interpretation, especially in subclinical thyroid disease. Laboratory information systems (LIS) have difficulty in reporting reference ranges (RR) in different pathophysiological situations. The work of the laboratory physician is important in developing and designing tools to identify these situations, and to make an appropriate interpretation of the results.</p></div><div><h3>Objective</h3><p>To determine whether the change in the LIS in our department and the issue of the laboratory report with the interpretation of the results, had an impact on the identification and monitoring of thyroid dysfunction in pregnant women in our area.</p></div><div><h3>Material and methods</h3><p>A retrospective cross-sectional study was carried out by analysing the results of all first-trimester pregnant women and those on whom thyroid tests had been requested in the following six months. The pregnant women were divided into two groups, before and after the change of the LIS.</p></div><div><h3>Results</h3><p>Follow-up percentages were similar in the two groups, except when TSH was abnormal for pregnant women and normal for the general population, that is, when there was no asterisk.</p></div><div><h3>Conclusions</h3><p>The RRs established for the normal population do not identify sub-clinical thyroid disease in pregnant women. The active intervention of the laboratory physician is essential in the evaluation of these results. In our study, more than 50% of the pregnant women with sub-clinical hypothyroidism benefited from the strategy introduced.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2018.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90215139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

El papel del laboratorio clínico en la medicina personalizada: situación actual y retos futuros 临床实验室在个性化医疗中的作用:现状和未来的挑战

Revista del Laboratorio Clínico Pub Date : 2018-10-01 DOI: 10.1016/j.labcli.2017.11.006

Maria Concepción Alonso-Cerezo , Emilio José Laserna Mendieta , Gema María Varo Sánchez , Marta Molina Romero , María Orera Clemente

引用次数: 1

Comparación de dos métodos para la cuantificación en suero de subclases de inmunoglobulinas 两种血清免疫球蛋白亚类定量方法的比较

Revista del Laboratorio Clínico Pub Date : 2018-10-01 DOI: 10.1016/j.labcli.2018.04.001

Ana Victoria Espinosa , Ana Navas , Juan Molina , Silvia Lagarcha , Rafael Solana , Corona Alonso

{"title":"Comparación de dos métodos para la cuantificación en suero de subclases de inmunoglobulinas","authors":"Ana Victoria Espinosa , Ana Navas , Juan Molina , Silvia Lagarcha , Rafael Solana , Corona Alonso","doi":"10.1016/j.labcli.2018.04.001","DOIUrl":"10.1016/j.labcli.2018.04.001","url":null,"abstract":"<div><h3>Background and aim</h3><p>The quantification of serum immunoglobulins, and particularly of IgG and IgG subclasses, is of interest for the diagnosis of numerous diseases. The usual detection methods provide different results according to the analyser used. The aim of this study was to compare the results obtained with two different analysers in the measurement of the concentration of IgG and IgG subclasses.</p></div><div><h3>Material and methods</h3><p>A total of 116 serum samples, regardless of the clinical diagnosis of the patients to whom the samples belonged, were analysed. The analyses were performed on a BNII<sup>®</sup> System (Siemens Healthcare GmbH, Germany) and Optilite<sup>®</sup> system (The Binding Site Group Ltd., Birmingham).</p></div><div><h3>Results</h3><p>The correlation between total IgG concentration (mg/dl) and the sum of the individual IgG subclasses detected was higher using the Optilite<sup>®</sup> analyser (0.976 vs. 0.866). The percentage of agreement between assays ranged from 43% to 71%, with the lower limit being for the IgG3 agreement. An absence of the usual IgG subclass physiological proportion (IgG1>IgG2>IgG3>IgG4) was detected using BNII<sup>®</sup>. These findings were a due to the significantly lower proportion of IgG3 obtained by BNII<sup>®</sup> compared to Optilite<sup>®</sup> (<em>P</em><.001), whereas the IgG4 concentration was not significantly different between analysers (<em>P</em>  <!-->.117).</p></div><div><h3>Conclusions</h3><p>Differences between the results obtained with the two different methods suggest that they should not be interchangeable, and that each clinical laboratory should only use one type of analyser. The reference ranges should be standardised according to the results obtained.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2018.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77166326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comentarios al Documento de consenso sobre la implementación de la secuenciación masiva de nueva generación en el diagnóstico genético de la predisposición hereditaria al cáncer 对关于在遗传性癌症易感性的遗传诊断中实施新一代大规模测序的共识文件的评论

Revista del Laboratorio Clínico Pub Date : 2018-10-01 DOI: 10.1016/j.labcli.2018.05.001

Orland Diez Gibert, la Comisión de Genética de la SEQCML

引用次数: 0

Evolución del estudio de la tuberculosis mediante Quantiferon Gold 用Quantiferon Gold进行结核病研究的进展

Revista del Laboratorio Clínico Pub Date : 2018-10-01 DOI: 10.1016/j.labcli.2018.01.001

Ángel San Miguel Hernández, Patricia de la Fuente Alonso

{"title":"Evolución del estudio de la tuberculosis mediante Quantiferon Gold","authors":"Ángel San Miguel Hernández, Patricia de la Fuente Alonso","doi":"10.1016/j.labcli.2018.01.001","DOIUrl":"10.1016/j.labcli.2018.01.001","url":null,"abstract":"<div><p>Tuberculosis is an infection caused by species of the <em>Mycobacterium tuberculosis</em> complex. It is a contagious disease with a high morbidity and mortality. It is mandatory to notify it, and its monitoring is carried out by the National Network for Epidemiological Surveillance, the European Centre for Disease Prevention and Control (ECDC), and the European Regional Office of the WHO.</p><p>The usual technique for diagnosis is the tuberculin test. This includes the intradermal injection of a purified protein derivative (PPD), which triggers a hypersensitivity reaction if the individual has been in contact with the substance previously.</p><p>In order to provide a more specific and safe diagnosis, new diagnostic methods have been developed based on the in vitro quantification of the cellular immune response, known generically as ‘interferon gamma release assays’ (IGRA), which detect the release of interferon-gamma by the sensitised T cells when subjected to different mycobacterial antigens.</p><p>There are currently two IGRAs being marketed for the in vitro diagnosis of tuberculosis infection QuantiFERON-TB Gold In-Tube (Cellestis<sup>®</sup>, QIAGEN) and T-SPOT.TB (Oxford Immunotec<sup>®</sup>). The aim is to present a short review on the use of the Quantiferon method in the diagnosis of tuberculosis infection.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2018.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78445275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Next generation sequencing for rapid diagnosis of a rare early onset spastic paraplegia: A novel pathological variant in FA2H gene 用于快速诊断罕见的早发性痉挛性截瘫的下一代测序:FA2H基因的一种新的病理变异

Revista del Laboratorio Clínico Pub Date : 2018-10-01 DOI: 10.1016/j.labcli.2018.01.004

M. Cristina González-González , Miriam Gutierrez , Cristina Castaño de la Mota , Nuria Muñoz Jareño , Severino Gonzalez , Fernando Cava

{"title":"Next generation sequencing for rapid diagnosis of a rare early onset spastic paraplegia: A novel pathological variant in FA2H gene","authors":"M. Cristina González-González , Miriam Gutierrez , Cristina Castaño de la Mota , Nuria Muñoz Jareño , Severino Gonzalez , Fernando Cava","doi":"10.1016/j.labcli.2018.01.004","DOIUrl":"10.1016/j.labcli.2018.01.004","url":null,"abstract":"<div><h3>Introduction</h3><p>Hereditary spastic paraplegia is a group of inherited neurological disorders with predominant manifestations of lower extremity weakness and severe spasticity. This is a genetically heterogeneous disorder very difficult to distinguish clinically with many genes described. Few patients with this condition have been previously reported.</p></div><div><h3>Patient and methods</h3><p>We present a case of a 5 years old girl, born from consanguineous parents, with severe ataxia and progressive spasticity of low limbs. Due to the severity of the symptoms and the need for early diagnosis, next generation sequencing study of 37 genes implicated in spastic paraplegia was performed.</p></div><div><h3>Results</h3><p>A novel pathological variant in <span><em>FA2H</em></span> gene was discovered. Father, mother and brother were heterozygous carriers.</p></div><div><h3>Conclusions</h3><p>Spastic paraplegia due to mutations in <em>FA2H</em> is an under diagnosed condition, and it should always be considered in childhood onset of progressive pyramidal dysfunction. Next Generation Sequencing allows a simultaneous analysis of many genes, enables a fast diagnosis in complex disorders.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2018.01.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74696933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Procedimiento para el estudio de interferencias exógenas en la medición de magnitudes biológicas. Documento técnico (2017) 生物量测量中外源性干扰研究的程序。白皮书(2017)

Revista del Laboratorio Clínico Pub Date : 2018-07-01 DOI: 10.1016/j.labcli.2017.09.005

Rosa M. López Martínez, Raúl Rigo Bonnin, M. José Andrés Otero, Francesca Canalias Reverter, Ruth Cano Corres, Sara Esteve Poblador, F. Javier Gella Tomás, Bernardino González de la Presa, Inmaculada Pérez de Algaba Fuentes

引用次数: 1

Valoración de la enfermedad por hígado graso no alcohólico desde el laboratorio clínico 临床实验室对非酒精性脂肪肝疾病的评估

Revista del Laboratorio Clínico Pub Date : 2018-07-01 DOI: 10.1016/j.labcli.2017.10.004

Iria Cebreiros López, José Antonio Noguera Velasco

引用次数: 0

Estudio prospectivo de la incidencia de patógenos genitales oportunistas y estrictos que crecen en medios de cultivo artificiales 在人工培养基中生长的机会性和严格性生殖病原体发生率的前瞻性研究

Revista del Laboratorio Clínico Pub Date : 2018-07-01 DOI: 10.1016/j.labcli.2017.11.009

Antonio Sorlózano-Puerto , Paula Esteban-Sanchís , Víctor Heras-Cañas , Jorge Fernández-Parra , José María Navarro-Mari , José Gutiérrez-Fernández

{"title":"Estudio prospectivo de la incidencia de patógenos genitales oportunistas y estrictos que crecen en medios de cultivo artificiales","authors":"Antonio Sorlózano-Puerto , Paula Esteban-Sanchís , Víctor Heras-Cañas , Jorge Fernández-Parra , José María Navarro-Mari , José Gutiérrez-Fernández","doi":"10.1016/j.labcli.2017.11.009","DOIUrl":"10.1016/j.labcli.2017.11.009","url":null,"abstract":"<div><h3>Background and objective</h3><p>Purulent or exudative genitourinary infections are a frequent reason for consultation in primary and specialized health care. The objective of this study was to determine the incidence of the microorganisms most commonly involved in the development of bacterial vaginosis, cervicitis, urethritis, vulvovaginitis, and balanitis in a general population attending 2tertiary level hospitals in the province of Granada (Spain).</p></div><div><h3>Patients and methods</h3><p>All the samples received for the microbiological diagnosis of acute lower genital tract infection between February and May 2015 were analysed following a standard protocol. Detection of the microorganisms in the samples was performed by cultivation in artificial media or nucleic acid hybridisation techniques (Affirm VPIII).</p></div><div><h3>Results</h3><p>The analysis included a total of 2,017 samples, obtained from 1,722 different patients (1626 women and 96 men).. The presence of at least one microorganism with clinical significance was detected in 772 patients (44.8%; 745 women and 27 men). Among the women, the most frequent microorganism more found was <em>Gardnerella vaginalis</em>, present in the 26.7%, followed by <em>Candida albicans</em> (20.0%), <em>Trichomonas. vaginalis</em> (1.0%), and <em>Ureaplasma urealyticum</em> (0.4%). <em>Neisseria gonorrhoeae</em> and <em>Haemophilus</em> spp. were detected in 10.4% and 6.3% of samples of male origin, respectively.</p></div><div><h3>Conclusions</h3><p>In the studied population, bacterial vaginosis by <em>G. vaginalis</em>, vulvovaginitis by <em>Candida</em> spp., trichomoniasis, gonorrhea, and urethritis by <em>Ureaplasma</em> spp. and <em>Haemophilus</em> spp., were the most frequent exudative genital infections.</p></div>","PeriodicalId":101105,"journal":{"name":"Revista del Laboratorio Clínico","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.labcli.2017.11.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75386295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9