用薄膜密封管保存的分析物与用塑料盖密封的分析物的稳定性

Juan Antonio Verna, Fernando Daniel Ventimiglia, Lorena Maydana, Ana Aristimuño, Jorge José Bruno, Liliana Elena D’Agostino
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引用次数: 0

摘要

实验室必须能够保证储存的患者样品的稳定性,以确认结果或解释医疗请求时的错误。本研究的目的是评估用薄膜和塑料盖密封的管中保存血液分析物的稳定性。材料和方法共24份血液样本被放入两根管中;一组用薄膜密封,另一组用塑料盖密封。使用罗氏Cobas c 501自动分析仪连续7天测量26例临床化学分析物。样品在2-8℃的冰箱中保存,计算总误差和变异系数,并与实验室的质量要求进行比较。结论薄膜封管比塑料封管的变化更大,酶在4天内保持在质量要求内,除铁外,离子仅在1天内稳定。除葡萄糖、尿酸、总蛋白和白蛋白外,代谢产物在7天内稳定。建议每个实验室必须根据其工作流程评估分析物的稳定性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Estabilidad de analitos conservados en tubo cerrado con film versus cerrado con tapa plástica

Introduction

The laboratory must be able to guarantee the stability of stored patient samples for confirmation of results or errors in the interpretation of the medical request. The aim of this study was to evaluate the stability of the blood analytes preserved in tubes sealed with film versus a plastic cap.

Material and methods

A total of 24 blood samples were aliquoted into two tubes; one sealed with film, and the other with a plastic cap. Twenty-six clinical chemistry analytes were measured for 7 consecutive days using a Roche Cobas c 501 autoanalyser. The samples were stored in a refrigerator between 2-8 C. The total error and coefficient of variation were calculated, and the results were compared against the quality requirements of the laboratory.

Conclusions

The variation was higher in the tubes sealed with film than in those with a plastic cap. Enzymes remained within the quality requirements for four days, and ions, apart from iron, were stable for only one day. The metabolites were stable for seven days excluding glucose, uric acid, total proteins, and albumin. It is suggested that each laboratory must evaluate the stability of analytes based on its workflow.

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