中华眼底病杂志最新文献

{"title":"Research status of antisense non-coding RNA in INK4 locus in long-chain non-coding RNA in diabetic retinopathy","authors":"ShuZe Chen, Zhengtong Yu, Zihong Wang, Xiaoqian Liang, Huimin Zhong, Min Fu","doi":"10.3760/CMA.J.ISSN.1005-1015.2020.01.019","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-1015.2020.01.019","url":null,"abstract":"The pathogenesis of diabetic retinopathy (DR) is complex. Antisense non-coding RNA (ANRIL) in the INK4 locus in long-chain non-coding RNA (lncRNA) is closely related to cell proliferation, differentiation, and individual development. It plays an important role in the dysplasia of retinal vascular endothelial cells and is a new field in the study of the pathogenesis of DR. According to the researches at present, ANRIL may plays its role in the occurrence and development of DR through the signal pathway of nuclear factor-κB and ROS/polyadenylation diphosphate ribose polymerase, and interact with p300, miR-200b, and EZH2 to regulating the expression and function of VEGF. Specific blocking ANRIL and its related pathways may become a new target in the treatment of DR. \u0000 \u0000 \u0000Key words: \u0000Diabetic retinopathy; Review; Long-chain non-coding RNA; Antisense non-coding RNA in the ink4 locus","PeriodicalId":10103,"journal":{"name":"Chinese Journal of Ocular Fundus Diseases","volume":"36 1","pages":"78-81"},"PeriodicalIF":0.0,"publicationDate":"2020-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48464510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lentivirus-mediated polypyrimidine bundle binding protein-associated splicing factor inhibits retinal neovascularization in mice of oxygen-induced retinopathy","authors":"Liangyu Huang, Yifeng Ke, Tingting Lin, Shaochong Bu, X. Ren, Min Jiao, Yong Wang, Li-ying Hu, Qiong Wang, Y. Hong, Xiaorong Li","doi":"10.3760/CMA.J.ISSN.1005-1015.2020.01.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-1015.2020.01.012","url":null,"abstract":"Objective \u0000To investigate the inhibitory effect of lentivirus-mediated polypyrimidine bundle binding protein-associated splicing factor (PSF) on retinal neovascularization (RNV) in mice model of oxygen-induced retinopathy (OIR). \u0000 \u0000 \u0000Methods \u0000One hundred and twelve 5-day-old C57BL/6J mice were randomly divided into normal control group, simple OIR model group, OIR model + lentivirus empty vector treatment group (Vec group) and OIR model + PSF lentivirus treatment group (PSF group), with 16, 32, 32 and 32 mice, respectively. When the mice were 7 days old, the mice in the normal control group were fed in a routine environment, and the mice in the OIR model group, Vec group and PSF group were established OIR model. The mice in the Vec group and PSF group were given an intravitreal injection of 1 μl of lentiviral vector and PSF lentivirus (titer 1×1011 TU/ml) at the age of 12 days. No injection was performed in the normal control group and simple OIR group. RNV was evaluated by counting the number of pre-retinal neovascular cells and analysis of non-perfusion area by immunofluorescent staining of the mouse retina. Real-time quantitative PCR was applied to detect the mRNA expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1). Western blot analysis was applied to detect the protein expression of Nrf2, HO-1 and PSF. \u0000 \u0000 \u0000Results \u0000Of the normal control group, simple OIR model group, Vec group and PSF group, the number of pre-retinal neovascular cell nuclei were 0.00, 14.36±5.50, 15.67±4.96, 8.13±2.09, the non-perfusion area were 0.00%, (35.71±2.81)%, (36.57±4.53)%, (15.33±4.75)%, respectively. The differences of the number of pre-retinal neovascular cell nuclei and non-perfusion area among 4 groups were significant (F=24.87, 165.70; P<0.05). Compared with the normal control group, there were more pre-retinal neovascular cell nucleis and larger non-perfusion area in the simple OIR model group and Vec group (P<0.05). Compared with the simple OIR model group and Vec group, there were lower pre-retinal neovascular cell nucleis and smaller non-perfusion area in the PSF group (P<0.05). Real-time quantitative PCR and Western blot showed that the mRNA expression of Nrf2, HO-1 (F=53.66, 83.54) and protein expression of Nrf2, HO-1 and PSF (F=58.38, 52.69, 24.79) among 4 groups were significant (P<0.05). The mRNA expression of Nrf2, HO-1 and protein expression of Nrf2, HO-1 and PSF in the simple OIR model group and Vec group decreased significantly than those in the normal control group (P<0.05). The mRNA expression of Nrf2, HO-1 and protein expression of Nrf2, HO-1 and PSF in the PSF group were increased significantly than those in the simple OIR model group and Vec group (P<0.05). model group and Vec group (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Intravitreal injection of lentivirus-mediated PSF inhibits RNV in mice model of OIR possibly through up-regulating the expression of Nrf2 and HO-1. \u0000 \u0000 \u0000Key words: \u0000Retinal neovascularization/prev","PeriodicalId":10103,"journal":{"name":"Chinese Journal of Ocular Fundus Diseases","volume":"36 1","pages":"53-59"},"PeriodicalIF":0.0,"publicationDate":"2020-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48709919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical features and research progress in autosomal recessive Best disease","authors":"Juejun Liu","doi":"10.3760/CMA.J.ISSN.1005-1015.2020.01.017","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-1015.2020.01.017","url":null,"abstract":"Autosomal recessive Best disease (ARB) is a rare clinical fundus disease caused by BEST1 mutation. The critical features of ARB are the presence of multifocal subretinal yellowish lesions, which corresponding to the hyperfluorescent spots on FAF, scattered over the posterior pole of the retina, absent of typical vitelliform lesions in the macula. Imaging of OCT is often manifested as subretinal or intraretinal fluid, and cystoid macular edema, and hypereflective focus at RPE level. EOG shows an absent or severely reduced light rise (decreased value of Arden), which often accompanied by reduction and delay of the rod and cone ERG. Some patients with ARB show hyperopia, short axial length and shallow anterior chambers, with a corresponding high incidence of angle-closure glaucoma. Though there isn't any effective therapeutic methods of ARB at present, prevention and treatment for its complications such as angle-closure glaucoma and choroidal neovascularization should be considered. Present study about ARB mainly focus on some retrospective cases, and ARB is often misdiagnosed with Best vitelliform macular dystrophy, central serous chorioretinopathy and other fundus diseases in clinic. A detailed understanding of the clinical features and genetic characteristics of ARB might be helpful in clinical diagnosis and treatment. Research with larger sample size are expected to further investigate the different stages of ARB and its developing process, the potential pathological mechanism, the relationship between genotype and phenotype, so as to improve the understanding of the disease. \u0000 \u0000 \u0000Key words: \u0000Best disease; Disease attributes; DNA mutational analysis; Review","PeriodicalId":10103,"journal":{"name":"Chinese Journal of Ocular Fundus Diseases","volume":"36 1","pages":"70-74"},"PeriodicalIF":0.0,"publicationDate":"2020-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41977537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress of the application of optical coherence tomography and angiography in the diagnosis and treatment of diabetic macular edema","authors":"Baoyi Liu, Dawei Yang, Qiaowei Wu, Honghua Yu","doi":"10.3760/CMA.J.ISSN.1005-1015.2020.01.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-1015.2020.01.016","url":null,"abstract":"Diabetic macular edema is the leading cause of central vision loss and even blindness in diabetic retinopathy. Compared to FFA, OCT can obtain the high-resolution 3D image quickly, easily to reflect the details of the tissue and realize the quantitative measurement. As a novel technology, OCT angiography (OCTA) can display microvascular structure from different layers of retina and choroid, having its advantage of quantifying the vessel density and the lesion area. By detecting fundus morphology, quantifying and quantitating the retinal vessels and vessel density, the combination of OCT and OCTA could play a guiding role in diagnosis, classification, treatment and prognosis of diabetic macular edema. \u0000 \u0000 \u0000Key words: \u0000Tomography, optical coherence; Macular edema; Diabetic retinopathy/complications; Review","PeriodicalId":10103,"journal":{"name":"Chinese Journal of Ocular Fundus Diseases","volume":"36 1","pages":"65-69"},"PeriodicalIF":0.0,"publicationDate":"2020-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42404319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The image characteristics of optical coherence tomography in patients of acquired immunodeficiency syndrome with cytomegalovirus retinitis","authors":"Shengnan Wang, Huiyu Sun, Fei-fei Mao, Dan Li","doi":"10.3760/CMA.J.ISSN.1005-1015.2020.01.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-1015.2020.01.002","url":null,"abstract":"Objective \u0000To observe the image characteristics of OCT in patients of acquired immunodeficiency syndrome (AIDS) with cytomegalovirus retinitis (CMVR). \u0000 \u0000 \u0000Methods \u0000Thirty-nine eyes of 26 patients of AIDS with CMVR diagnosed in Department of ophthalmology of Beijing Ditan Hospital Capital Medical University from January 2015 to December 2017 were included in this study. All the patients were males, with the mean age of 33.12±9.87 years. All the patients underwent the OCT examination by Spectralis HRA+OCT. The locations of scanning were macular, optical papilla and posterior pole of retina with retinitis. Typical images were saved and analyzed. \u0000 \u0000 \u0000Results \u0000The OCT pathological changes of CMVR included increase of retinal thickness and reflex of retina, indiscernible retinal layers, irregularity or absent external limiting membrane and/or ellipsoid zone, hyperreflective spots, vitreous cells. Among 39 eyes, there were 6 eyes with strong point-like reflection in the outer layer of retina around the lesion, 31 eyes (79.49%) with strong point-like reflection in the full layer of retina, 25 eyes (64.10%) with lesion involved macular area, 34 eyes (87.17%) with vitreous cells. \u0000 \u0000 \u0000Conclusions \u0000OCT images of the eyes with AIDS with CMVR were characterized by lesions involving the whole retina. Absent ellipsoid zone or structural changes can be seen in the affected areas and peripheral areas of the lesion. \u0000 \u0000 \u0000Key words: \u0000Acquired immunodeficiency syndrome/complications; Cytomegalovirus retinitis/diagnosis; Tomography, optical coherence","PeriodicalId":10103,"journal":{"name":"Chinese Journal of Ocular Fundus Diseases","volume":"36 1","pages":"5-9"},"PeriodicalIF":0.0,"publicationDate":"2020-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45249058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visual acuity recovery after vitrectomy of idiopathic macular hole and its influencing factors: a longitudinal study","authors":"Ying Wang, Q. Han","doi":"10.3760/CMA.J.ISSN.1005-1015.2020.01.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-1015.2020.01.009","url":null,"abstract":"Objective \u0000To observe the dynamic characteristics of visual acuity after vitrectomy in different sizes of idiopathic macular hole, and analyze the influencing factors. \u0000 \u0000 \u0000Methods \u0000A retrospective study. From August 2016 to June 2018, 302 patients (302 eyes) with monocular idiopathic macular hole who underwent 25G vitrectomy combined with the internal limiting membrane peeling in Tianjin Eye Hospital were included in the study. There were 130 males and 172 females, with the mean age of 63.36±6.91 years. There were 158 left eyes and 144 right eyes. The logMAR BCVA was 1.13±0.45. The minimum diameter (422.92±211.73 μm) and basal diameter (835.47±366.42 μm) of macular hole and choroid thickness under fovea (244.84±60.68 μm) were measured by OCT. According to the minimum diameter, the holes were divided into small hole group (≤ 250 μm), middle hole group (>250 μm and ≤400 μm) and large hole group (>400 μm). The logMAR BCVA at 1, 3 and 6 months after surgery in 3 groups were observed. Two-factor repeated measure ANOVA was used to compare the visual acuity of the 3 groups. The generalized estimation equation (GEE) was used to analyze the influencing factors of postoperative visual recovery. \u0000 \u0000 \u0000Results \u0000One month after surgery, all the holes were closed. One, 3 and 6 months after surgery, the logMAR BCVA were 0.33±0.25, 0.23±0.18, 0.16±0.17 in the small hole group; the logMAR BCVA were 0.46±0.25, 0.35±0.26, 0.27±0.28 in the middle hole group; the logMAR BCVA were 0.81±0.51, 0.61±0.48, 0.53±0.37 in the large hole group. Through repeated measurement variance analysis of two factors, it was found that there was an interaction between different groups and different time nodes (F=23.133, P 0.05). In the large hole group, among preoperative, 1 months after surgery, 3 months after surgery, the visual acuity difference was statistically significant (P 0.05). The results of GEE analysis showed that hole size (χ2=4.17, P=0.04), basal diameter (χ2=7.25, P=0.01), disease course (χ2=19.26, P=0.00), and choroid thickness (χ2=4.19, P=0.04) were the influencing factors of postoperative visual acuity. \u0000 \u0000 \u0000Conclusions \u0000After vitrectomy of macular holes of different sizes, the visual recovery trend is different. The visual recovery of small holes and middle holes is faster and basically restored at 1 month. The large holes requires a slow recovery process and stabilizes vision at 3 months. Hole size, basal diameter, course of disease and choroid thickness are the influencing factors of visual acuity recovery. \u0000 \u0000 \u0000Key words: \u0000Retinal perforations/surgery; Vitrectomy; Root cause analysis; Longitudinal study","PeriodicalId":10103,"journal":{"name":"Chinese Journal of Ocular Fundus Diseases","volume":"36 1","pages":"38-41"},"PeriodicalIF":0.0,"publicationDate":"2020-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45254176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanisms for mutual interactions between microglial cell and Müller cell in ischemic retinopathy","authors":"Shuang Gao","doi":"10.3760/CMA.J.ISSN.1005-1015.2020.01.018","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-1015.2020.01.018","url":null,"abstract":"Ischemic retinopathy, resulting in multiple lesions like microvasculature damage, inflammation and neovascularization, is a major contributor of vision damage. In these pathological changes, retinal glia cannot be ignored in the development of retinopathy. They constitute a highly versatile population that interacts with various cells to maintain homeostasis and limit disease. Therefore, glial activation and gliosis are strikingly ubiquitous responses to almost every form of retinal disease. Both of microglial cells and Muller cells are major intrinsic retinal glial cells and they are in close relationship, which means they can influence each other, make joint action or even become interdependent. They exhibit morphological and functional changes to have an impact on degree of retinal injury through different responses, which mediated by glial cells are important not only for course of disease progression, but also for the maintenance of neuronal and photoreceptor survival. Thus, defining the mechanisms that underlie communications between microglial cells and Muller cells could enable the development of more selective therapeutic targets, with great potential clinical applications. \u0000 \u0000 \u0000Key words: \u0000Microglia; Muller cell; Review; Ischemic retinopathy","PeriodicalId":10103,"journal":{"name":"Chinese Journal of Ocular Fundus Diseases","volume":"36 1","pages":"74-78"},"PeriodicalIF":0.0,"publicationDate":"2020-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49034778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The value of color Doppler ultrasound in the detection of macular diseases in age-related cataract patients","authors":"Lin Shen, Wenli Yang, Dong-jun Li, Zi-yang Wang, Wei Chen, Qi Zhao, Yifeng Li, R. Cui","doi":"10.3760/CMA.J.ISSN.1005-1015.2020.01.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-1015.2020.01.008","url":null,"abstract":"Objective \u0000To observe the application value with color Doppler flow imaging (CDFI) in the detection of macular diseases in senile cataract. \u0000 \u0000 \u0000Methods \u0000A cross-sectional descriptive study. From December 1 to December 30, 2017, 720 senile cataract patients (1380 eyes) diagnosed in Beijing Tongren Eye Center were included in this study. There were 330 males (629 eyes) and 390 females (751 eyes), with the mean age of 70.35±19.05 years. There were 716 right eyes and 664 left eyes, with the visual acuity 0.05-0.8. The fundus of all the patients can be observed. All patients were examined by OCT using Cirrus HD-Model 5000 produced by German Carl Zeiss company and CDFI using MyLab90 color Doppler ultrasound system produced by ESAOTE S.P.A. Comparing the detection rate of macular lesions between the two methods by using χ2 test. Using statistical methods to evaluate diagnostic tests, a crosstabulation was made to compare the consistency of frequency-domain OCT and CDFI in the diagnosis of macular lesions. Logistic regression analysis of correlation between detection of CDFI macular lesions and central foveal thickness (CFT), average thickness (AT), with or without macular anterior membrane, retinal folds, retinal cystoid edema, hard exudation, macular hole or lamellar macular hole, superficial detachment of neuroepithelium, detachment of pigment epithelium, vitreous macular traction. \u0000 \u0000 \u0000Results \u0000Two hundreds and thirty-nine eyes (17.3%) showed macular disease by OCT, and 161 eyes (11.7%) showed the abnormal echo in the macular region by CDFI. The detection rate of the macular disease between two methods were statistically significant (χ2=851.661, P<0.001), however, the consistency was well (κ=0.766, P=0.000). Logistic analysis showed that the detection rate of macular lesions was related to retinal folds, cysts in retina and CFT (r=1.396, 1.041, 0.12; P<0.01). \u0000 \u0000 \u0000Conclusion \u0000CDFI can effectively detect macular lesions that affect the visual prognosis of senile cataract patients. \u0000 \u0000 \u0000Key words: \u0000Ultrasonography, doppler, color; Macular degeneration; Cataract","PeriodicalId":10103,"journal":{"name":"Chinese Journal of Ocular Fundus Diseases","volume":"36 1","pages":"33-37"},"PeriodicalIF":0.0,"publicationDate":"2020-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49651834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of polypyrimidine bundle-binding protein-related splicing factor on retinal pigment epithelial cell injury induced by advanced glycation end products","authors":"Chen Qi, Hui Zhang, Tingting Lin, Yifeng Ke, X. Ren, Shaochong Bu, Liangyu Huang, Yong Wang, Min Jiao, Li-ying Hu, Qiong Wang, Y. Hong, Xiaorong Li","doi":"10.3760/CMA.J.ISSN.1005-1015.2020.01.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-1015.2020.01.011","url":null,"abstract":"Objective \u0000To observe the protective effect of polypyrimidine bundle-binding protein-related splicing factor (PSF) over-expression on RPE cell injury induced by advanced glycation end products (AGEs). \u0000 \u0000 \u0000Methods \u0000The human RPE cells cultured in vitro were divided into three groups: normal control group (N group), blank control group (N + AGEs group), empty vector control group (Vec + AGEs group), and PSF high expression group (PSF + AGEs). group). RPE cells in N group were routinely cultured; RPE cells in N + AGEs group were only transfected but did not introduce any exogenous genes combined with AGEs induction; Vec +AGEs group and PSF + AGEs group were transfected with pcDNA The empty vector or pcDNA-PSF eukaryotic expression plasmid was introduced into RPE cells and induced by AGEs. Except the N group, the other 3 groups of cells were transfected accordingly, and were stimulated with 150 μg/ml AGEs for 72 h after 24 h. HE staining and Hoechst 33258 staining were used to observe the effect of high PSF expression on the morphological changes of RPE cells; ROS level detection was used to analyze the effect of PSF high expression on the ROS expression of RPE cells induced by AGEs; MTT colorimetric method was used to detect the high PSF expression Effects on the viability of RPE cells; Western blot was used to detect the effects of different time and dose of PSF on the expression of heme oxygenase 1 (HO-1). \u0000 \u0000 \u0000Results \u0000HE staining and Hoechst 33258 staining observation showed that the cells in group N were full in shape, the nucleus was round, the cytoplasm was rich, and the staining was uniform; the cells in N + AGEs group and Vec + AGEs group were reduced in size, the eosinophilic staining was enhanced, and the nucleus was densely densely stained. Pyrolysis and even fragmentation; the morphology of cells in the PSF + AGEs group was still full, the cytoplasm staining was more uniform, and the nucleus staining was uniform. The results of MTT colorimetry showed that high expression of PSF can effectively improve the viability of RPE cells, but this effect can be effectively antagonized by ZnPP, and the difference is statistically significant (F=33.26, P<0.05). DCFH-DA test results showed that compared with the N + AGEs group and Vec + AGEs group, the ROS production in PSF + AGEs group decreased, the difference was statistically significant (F=11.94, P<0.05). Western blot analysis showed that PSF protein upregulated HO-1 expression in a time- and dose-dependent manner. The relative expression level of HO-1 at 24, 48, and 72 h after PSF protein was significantly higher than that at 0 h, and the difference was statistically significant (F=164.91, P<0.05). The relative expression level of HO-1 under the action of 0.1, 0.5, 1.0, 1.5, and 2.0 μg PSF protein was significantly higher than 0.0 μg, and the difference was statistically significant (F=104.82, P<0.05). \u0000 \u0000 \u0000Conclusion \u0000PSF may inhibit the production of ROS by up-regulating the expression of H","PeriodicalId":10103,"journal":{"name":"中华眼底病杂志","volume":"36 1","pages":"46-52"},"PeriodicalIF":0.0,"publicationDate":"2020-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43091487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative analysis of optic disc structure and retinal nerve fiber layer thickness in patients with different degrees of Parkinson's disease","authors":"Ying Zhao, Wei-jia Dai","doi":"10.3760/CMA.J.ISSN.1005-1015.2020.01.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1005-1015.2020.01.004","url":null,"abstract":"Objective \u0000To observe the changes of optic disc structure and retinal nerve fiber layer thickness (RNFL) in patients with different degrees of Parkinson's disease (PD). \u0000 \u0000 \u0000Methods \u0000Thirty eyes of 30 patients with primary PD and 20 eyes of 20 healthy subjects (control group) in Xuanwu Hospital of Capital Medical University from October 2016 to October 2017 were enrolled in this study. The patients were divided into mild to moderate PD group (15 eyes of 15 patients) and severe PD group (15 eyes of 15 patients). All the patients underwent OCT examination. The optic disc area, cup area, C/D area ratio, rim volume, disc volume, cup volume, rim area, C/D area, linear C/D, vertical C/D, the thickness of average RNFL, superior, inferior, temporal upper (TU), superior temporal (ST), superior nasal (SN), nasal upper (NU), nasal lower (NL), inferior nasal (IN), inferior temporal (IT), temporal lower (TL) quadrant RNFL thickness. Analysis of variance was performed for comparison among three groups. Minimum significant difference t test was performed for comparison between two groups. \u0000 \u0000 \u0000Results \u0000Optic disc structure parameters: there was no significant difference in the area of optic disc between the three groups (F=1.226, P>0.05). The other optic disc parameters were significantly different in the three groups (F=5.221, 5.586, 6.302, 5.926, 5.319, 5.404, 5.861, 6.603; P 0.05). The average thickness of RNFL, TU, IN, IT and TL in patients of the mild to moderate PD group and severe PD group were less than that in the control group (P<0.05). The thickness of the average RNFL, TU, IN, IT and TL in patients of the severe PD group were less than that in the mild to moderate PD group (P<0.05). With the increase of PD severity, the RNFL of TL and TU thinned most significantly. \u0000 \u0000 \u0000Conclusions \u0000With the increase of the severity of PD, the optic disc structure and RNFL thickness changes obviously, showing reduced optic disc area and volume, enlarged cup area and volume significantly enlarged C/D ratio. The average RNFL thickness of PD patients is significantly thinner than that of the controls, and it is the most obvious in the TU and TL quadrant. \u0000 \u0000 \u0000Key words: \u0000Parkinson's disease; Optic disk; Nerve fibers; Tomography, optical coherence","PeriodicalId":10103,"journal":{"name":"中华眼底病杂志","volume":"36 1","pages":"15-19"},"PeriodicalIF":0.0,"publicationDate":"2020-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47646610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}