Plant Nano Biology最新文献

{"title":"Calcareous soil modified with metallic and organic ZnO nanoparticles limits photosynthetic pigment accumulation and macronutrient uptake in Swiss chard (Beta vulgaris var. cicla)","authors":"Eddaliz García-Reyes ,&nbsp;Josué I. García-López ,&nbsp;Sonia N. Ramírez-Barrón ,&nbsp;Antonio Flores-Naveda ,&nbsp;Perpetuo Álvarez-Vázquez ,&nbsp;Agustín Hernández-Juárez ,&nbsp;Enrique Díaz Barriga-Castro","doi":"10.1016/j.plana.2024.100108","DOIUrl":"10.1016/j.plana.2024.100108","url":null,"abstract":"<div><div>Previous studies on the effects of zinc oxide nanoparticles have mainly examined controlled agricultural settings, failing to consider their behavior in real agricultural soil. As a result, our knowledge of their environmental impact remains incomplete. This study was specifically developed to observe the comparative effects of metallic zinc oxide nanoparticles, zinc sulfate, and zinc oxide green nanoparticles at different concentrations (25, 50, 75 and 100 mg of Zn kg⁻¹ of soil) on growth parameters, the mineral content (N, P, K and Zn) in root and leaf, the content of chlorophyll a (CHLa), b (CHLb), and total (CHLt), and carotenoids in Swiss chard plants grown in calcareous soil. Leaf area and dry root weight increased by 23.27 % and 46.20 %, respectively, in zinc sulfate modified soil. Total chlorophyll and carotenoids also increased by 40.12 % and 32.59 %. The concentration of N, P, K and Zn in roots was 2.89, 1.74, 1.70 and 1.52 times higher, while in leaves, the concentration was 1.48, 1.44, 1.76 and 2.22 times higher in plants grown with zinc sulfate. The effects on plant growth can be attributed to the type of fertilizer used and its influence on macronutrient absorption in the soil. The utilization of zinc sulfate as a soil treatment led to elevated absorption of macronutrients and zinc, suggesting a connection between the fertilizer type and the crop’s agronomic and physiological reactions.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100108"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142651190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant enzymatic activity as an indicator of nano-TiO2 exposure in rice ecosystems","authors":"Raviteja Machanuru ,&nbsp;Manoj Shrivastava ,&nbsp;Renu Singh ,&nbsp;Bhupinder Singh ,&nbsp;Debashis Chakraborty ,&nbsp;Pooja Lakshmidevarahalli Ramalingappa ,&nbsp;Mahesh Narayan","doi":"10.1016/j.plana.2024.100117","DOIUrl":"10.1016/j.plana.2024.100117","url":null,"abstract":"<div><div>The widespread use of nano-titanium dioxide (nano-TiO₂) has raised concerns about its environmental impact, particularly in soil-plant systems. This study investigates the effects of nano-TiO₂ on rice (<em>Oryza sativa</em> cv. PB1121) growth and enzymatic activities, compared to bulk TiO₂, through a pot culture experiment. Eight doses of Ti were applied: six as soil treatments (0, 2.5, 5, 10, 25, and 50 mg Ti kg⁻¹) and two as foliar treatments (0.05 % and 0.1 %). Results showed that grain yield peaked at 25 mg Ti kg⁻¹ soil for both nano and bulk TiO₂, while a 0.05 % foliar spray outperformed by 0.1 %. Titanium accumulated mostly in roots, followed by straw and grains. Nano-TiO₂ significantly increased antioxidant enzyme activities—catalase (CAT), superoxide dismutase (SOD), guaiacol peroxidase, and ascorbate peroxidase (APX)—and lipid peroxidation (measured as malondialdehyde) in rice roots and shoots, indicating oxidative stress. The findings suggest that plant enzymatic activity serves as an early indicator of nano-TiO₂ exposure, making it a valuable biomarker for environmental monitoring. However, higher Ti doses may inhibit plant growth depending on Ti source and concentration. Further studies should examine the effects of nano-TiO₂ of different sizes, shapes, and charges on various crops and soil types to validate these results and assess the broader implications for agricultural and environmental health. This research highlights the dual potential of nano-TiO₂ as both a growth enhancer and a stress-inducing agent, emphasizing the need for careful management in agricultural applications.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100117"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Larrea nitida extract-loaded nanodispersions as a novel bio-stimulant for tomato plants","authors":"Felipe Rocha ,&nbsp;Laura Svetaz ,&nbsp;Maximiliano Sortino ,&nbsp;Valeria Alina Campos-Bermudez ,&nbsp;Sebastián Pablo Rius","doi":"10.1016/j.plana.2024.100123","DOIUrl":"10.1016/j.plana.2024.100123","url":null,"abstract":"<div><div><em>Larrea nitida</em> Cav. (Zygophyllaceae) hydrophobic extract (LE) is rich in antimicrobial and antioxidant compounds. In our previous study, water-soluble nanodispersions without (PZ) and with LE (PZLE) were produced to facilitate their application in agriculture. The foliar treatment was carried out with water (control), PZ and PZLE thrice a week at concentrations of 33 and 100 <span><math><mrow><mi>m</mi><mi>g</mi><mo>.</mo><msup><mrow><mi>L</mi></mrow><mrow><mo>-</mo><mn>1</mn></mrow></msup></mrow></math></span> on 1-week-old tomato seedlings (<em>Solanum lycopersicum</em> L. variety <em>perita platensis</em>). PZLE at a concentration of 100 <span><math><msup><mrow><mi>mg</mi><mo>.</mo><mi>L</mi></mrow><mrow><mo>−</mo><mn>1</mn></mrow></msup></math></span> significantly induced a growth promotion effect and decreased the activity of the antioxidant enzymes guaiacol peroxidase (GPx) and catalase (CAT), probably due to the presence of antioxidant compounds in PZLE. However, 5 days after infection with <em>Pseudomona syringae</em> pv. tomato, the activity of CAT increased by 73 % compared to the control, as a response to the stress caused by the infection. Additionally, plants treated with PZLE at 33 and 100 <span><math><msup><mrow><mi>mg</mi><mo>.</mo><mi>L</mi></mrow><mrow><mo>−</mo><mn>1</mn></mrow></msup></math></span> exhibited reductions in infection symptoms of 44 % and 76 %, respectively. As for both concentrations of PZ, no significant differences were found when compared to the control, suggesting a protective effect of PZLE. The photosynthetic parameters of the plants, such as Phi2 (<span><math><mrow><msub><mrow><mi>φ</mi></mrow><mrow><mi>PSII</mi></mrow></msub><mo>)</mo></mrow></math></span>, non-photochemical quenching (NPQ) and the relative chlorophyll in the leaves of these plants remained unaffected, suggesting no detrimental effects of PZLE on photosynthetic efficiency. PZLE demonstrates potential as a bio-stimulant for tomato plants, offering a complementary approach to traditional agricultural inputs.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100123"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo studies of Vitex negundo-derived silver oxide nanoparticles against Meloidogyne incognita (Root-knot nematode) on tomato plants","authors":"Kanika Choudhary ,&nbsp;Deepak Sharma ,&nbsp;Dixit Sharma ,&nbsp;Pankaj Thakur ,&nbsp;Kushal Thakur ,&nbsp;Sunil Kumar","doi":"10.1016/j.plana.2024.100118","DOIUrl":"10.1016/j.plana.2024.100118","url":null,"abstract":"<div><div><em>Meloidogyne incognita</em> (<em>M. incognita</em>) is an important obligatory pest affecting a large number of horticultural crops. The pest is widely distributed in tropical, subtropical, and warmer regions of the world. <em>M. incognita</em> induces root gall formation that interferes with nutrient supply leading to stunted growth, wilting, chlorosis and significant drop in plant productivity. In recent years biogenic nanoparticles have emerged as an alternative approach for controlling agricultural pests. The current study investigates the nematicidal potential of green-synthesized silver oxide nanoparticles derived from <em>Vitex negundo</em> against second-stage juvenile (J2) and eggs of <em>M. incognita</em>. The maximum mortality (97.20 %) of <em>M. incognita</em> was found after 96 h of treatment. The lowest egg hatching (7.40 %) in comparison with the control (86.93 %) was found in 500 ppm concentration after six days of exposure. The bioassay results revealed that both juveniles and eggs generally showed a concentration-dependent response. The <em>in vivo</em> treatment with biogenic nanoparticles showed reduced gall formation and increased plant growth in tomato plants. Maximum reduction in the galls and plant growth parameters was observed in higher concentrations (1000 ppm) of VND-AgONPs after 60 days compared to untreated inoculated control. To best of our knowledge, this is the first report of the nematicidal efficacy of VND-AgONPs against J2 and eggs of <em>M. incognita.</em> These biogenic nanoparticles could be recommended for managing Root-knot nematodes due to their higher efficacy, cost-effectiveness, and environmentally safe nature.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100118"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of zinc oxide nanoparticles on biosynthesis of thymoquinone in cell cultures of Nigella sativa","authors":"Ambreen ,&nbsp;Mubarak Ali Khan ,&nbsp;Afzal Raza ,&nbsp;Reema Yousaf ,&nbsp;Huma Ali ,&nbsp;Hadeer Darwish","doi":"10.1016/j.plana.2024.100109","DOIUrl":"10.1016/j.plana.2024.100109","url":null,"abstract":"<div><div>The rising market interest for <em>Nigella sativa</em> (Black seeds) necessitates the development of cultivation strategies to enhance metabolites production. Zinc oxide nanoparticles (ZnO-NPs) have drawn global attention as efficient and bio safe elicitors for <em>in vitro</em> cultures, to enhance secondary metabolites production in medicinal plants. In this study, ZnO-NPs were utilized for establishment of callus and cell cultures in black seeds for the first time. Hypocotyl explants were cultured on Murashige and Skoog (MS) media with varying levels of ZnO-NPs, resulted in callus induction and biomass formation. Optimal response in callus growth parameters were observed when explants were grown on MS media supplemented with 60 mg/L ZnO-NPs, resulting in 71.2 % callus induction frequency, 28.2 g/L fresh biomass, 9.7 g/L dry biomass, and 63 % water content. A substantial increase in callus growth was observed when ZnO-NPs were combined with 6-Benzylaminopurine (BA) at ratio of 45:1.5 mg/L, resulting in 91.2 % callus induction frequency and 42.2 g/L fresh biomass. In cell suspension cultures, ZnO-NPs alone at 45 mg/L produced optimum callus biomass (60.9 g/L). However, in combination with BA, callus biomass did not increase significantly in cell cultures. Maximum accumulation of total phenolic content (TPC: 26.8 mg GAE/g DW; Gallic acid equivalent dry weight) and total flavonoid content (TFC: 19.5 mg QE/g DW; Quercetin equivalent dry weight) was observed in cell cultures treated with higher concentration (70 mg/L) of ZnO-NPs in the 5th week of the growth curve. Moreover, ZnO-NPs incremented substantially the Phenylalanine lyase (PAL), Superoxide dismutase (SOD) and Peroxidase (POD) enzyme activities in cell cultures. Nonetheless, Reverse Phase High Performance Liquid Chromatography (RP-HPLC) analysis indicated peak production of thymoquinone (168.5 mg/g FW) in cell cultures treated with 45 mg/L ZnO-NPs alone. This study offers a promising approach for commercial production of <em>Nigella sativa</em> biomass and bioactive metabolites.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100109"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of metal nanoparticles using plant growth promoting rhizobacteria and application in agriculture","authors":"Roohallah Saberi Riseh ,&nbsp;Mozhgan Gholizadeh Vazvani","doi":"10.1016/j.plana.2024.100111","DOIUrl":"10.1016/j.plana.2024.100111","url":null,"abstract":"<div><div>Nanotechnology holds significant promise for transforming the agricultural industry by enhancing efficiency and yield through the manipulation of materials at the nanoscale. Green synthesis of metal nanoparticles represents an innovative and environmentally friendly approach in agriculture that enhances crop yield and quality. Unlike traditional physical and chemical methods, which are costly and produce harmful by-products, green synthesis uses biological agents like plants, fungi, and bacteria, making the process more sustainable and cost-effective. Bacterial cells demonstrate superior efficiency in the green synthesis of nanoparticles compared to plant cells. Their effectiveness is attributed to their unique morphological features, surface structures, and the presence of exopolysaccharides, sugars, proteins, enzymes, and various functional groups (such as carboxyl, phosphate, and amide). Interaction between bacterial cells and nanotechnology presents a promising approach for advancing smart agriculture by enhancing soil fertility, plant protection, and nanoparticle synthesis efficiency. By entering the structure of a living organism as an external factor, nanoparticles can cause extensive changes in the physiology and function of the cell. Therefore, the presence of a biological mediating agent with potential mechanisms capable of reducing the negative effects of nanoparticles can increase their efficacy as a long-term factor and reduce concerns about the harmful effects of nanoparticles on nature and living cells. By activating the pathways of NADPH, glutathione, and biofilm formation, this living agent first moderates the harmful effects of nanoparticles and acts as a filter, then provides them for the cell as a healthy biological-nanotechnological agent through a process known as green synthesis. Integration of nanoparticles and PGPR offers a novel and efficient approach to increasing plant productivity and sustainability in agriculture.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100111"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142651236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of AgNPs production from Fusarium oxysporum H39 and its effectiveness as nanopesticides facing Pectobacterium carotovorum","authors":"Mayra Eleonora Beltrán Pineda ,&nbsp;Luz Marina Lizarazo Forero ,&nbsp;Cesar Augusto Sierra","doi":"10.1016/j.plana.2024.100104","DOIUrl":"10.1016/j.plana.2024.100104","url":null,"abstract":"<div><div>In response to the increasing shortage of agrochemicals in developing countries, which directly impacts farmers' livelihoods, this study investigates the synthesis of silver nanoparticles (AgNPs) employ the enzymatic extract from a native strain of <em>Fusarium oxysporum</em> H39. These nanoparticles were assessed for their antibacterial efficacy facing potato tuber soft rot caused by <em>Pectobacterium carotovorum</em>. The optimal conditions for AgNP synthesis were determined to be a concentration of 3 mM, a pH of 10, and a temperature of 27°C over a 24-hour period. Under these conditions, spherical nanoparticles with an average size of 12.3 ± 4.3 nm were produced. FTIR analysis indicated the presence of organic compounds on the surface of the AgNPs, as evidenced by bands corresponding to C-O, C-N, and C-C bonds. The nanoparticles demonstrated significant nanopesticide activity facing the phytopathogenic bacterium <em>P. carotovorum</em>, with minimum inhibitory concentrations (MICs) of 25 ppm and 50 ppm identified through microdilution and macrodilution assays, respectively. Additionally, in controlled substrate tests, preventive treatment of tubers with a 100 ppm dose of AgNPs significantly reduced the weight of macerated tissue, the primary symptom of the disease.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100104"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoemulsion edible coating for shelf-life improvement and quality control in perishable products","authors":"Diksha Thakur ,&nbsp;Paki Rana ,&nbsp;Shailesh Kumar Singh ,&nbsp;Manish Bakshi ,&nbsp;Sanjeev Kumar ,&nbsp;Sanjay Singh","doi":"10.1016/j.plana.2024.100114","DOIUrl":"10.1016/j.plana.2024.100114","url":null,"abstract":"<div><div>The perishable nature of a wide range of food commodities including horticultural produce is a challenge to global food and nutritional security and a hindrance in the target of zero hunger by 2030 (The United Nations Sustainable Development Goals). Nanoemulsion edible coating is an emerging technique for creating lipophilic active ingredient delivery systems to facilitate their integration with edible coatings applied over perishable horticultural produce. The current review provides insight into the formulation techniques, characteristics, stability, and application of nanoemulsion edible coatings to improve the quality and shelf-life of perishable horticultural produce. The nanoemulsion coatings on fruits and vegetables are analogous to modified atmosphere packaging, as these coatings create physical barriers to alter the gaseous exchange between the fruit’s internal and external atmosphere, increase the CO₂ concentration, lower the O₂ concentration, and reduce the respiration rate. The nanoscale droplets in the emulsion increase the surface area, allowing for improved coverage of the food surface and improving the barrier to prevent moisture loss, microbiological contamination, and oxidative deterioration and extending the freshness of fruits, vegetables, and other perishable foods. The nanoemulsion-based edible packaging/coating has the potential to revolutionize the food business by addressing issues such as food waste, sustainability, and consumer demand for healthier and longer-lasting products.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100114"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective chitosan-antioxidant treatments for extending cassava shelf-life evaluated using silver nanosensor","authors":"Adetoun Akitoye ,&nbsp;Greatness Olaitan ,&nbsp;Isaac Akinbulu ,&nbsp;Wesley Okiei","doi":"10.1016/j.plana.2024.100115","DOIUrl":"10.1016/j.plana.2024.100115","url":null,"abstract":"<div><div>Cassava is a highly resilient and versatile root crop that plays a crucial role in food security across many tropical regions. However, its short postharvest shelf-life results in significant losses, highlighting the need for effective strategies to extend its longevity. This study introduces a novel approach to monitoring and mitigating cassava deterioration by utilizing silver nanoparticle sensors to track hydrogen peroxide (H₂O₂) production, a key indicator of oxidative stress. Four economically viable cassava root cultivars were evaluated for their tolerance to postharvest physiological deterioration (PPD) and quality retention after treatment with various chitosan-antioxidant formulations. The results indicated that the chitosan-quercetin formulation (CS-Q) was the most effective, achieving the lowest average H₂O₂ reduction of 67.7 % compared to untreated samples, followed by chitosan-beta-carotene (CS-BC) at 62.4 %, chitosan-ascorbic acid (CS-AA) at 38.3 %, and chitosan (CS) at 34.8 %. These reductions in H₂O₂ suggest that chitosan-antioxidant treatments can effectively delay PPD and extend cassava's shelf life for up to six months, depending on the cultivar, seasonal factors, and specific antioxidant composition. The successful implementation of this innovation offers a promising solution to delay PPD and enhance the efficiency of the cassava supply chain. Furthermore, this study contributes to the advancement of electrochemical techniques for assessing PPD and demonstrates the potential of chitosan-based materials in improving the postharvest preservation of cassava and potentially other crops.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100115"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of silver nanochitosan in protecting wheat seeds from fungal infection and increasing growth parameters","authors":"Divya Chouhan ,&nbsp;Palash Mandal ,&nbsp;Chandrani Choudhuri ,&nbsp;Piyush Mathur","doi":"10.1016/j.plana.2024.100116","DOIUrl":"10.1016/j.plana.2024.100116","url":null,"abstract":"<div><div>Wheat is regarded as the prime source of dietary carbohydrate, protein in the majority of countries all over the world. Wheat growth, productivity and seed quality has been extensively hampered by the aggression of seed-borne pathogens during post-harvest storage. The present work focus on the efficacy of chitosan-based Ag²⁺ nanoparticles (Ag-CNPs) for the management of seed borne pathogens of wheat and their subsequent effects on growth, yield, and quality of harvested seeds. For this, healthy seeds of wheat were nanoprimed with Ag-CNPs and further inoculated with <em>A. flavus</em> so as to induce pathogenic stress. These seeds were sown in pots in a completely randomized block design and plants were raised from both nanoprimed and inoculated seeds, while plants were also raised from non-nanoprimed primed and uninoculated that served as control. Nanoprimed and pathogen inoculated seeds effectively increased vegetative growth such as (132.31 %), fresh weight (1.63-fold), dry weight (1.67-fold), and panicle length (137.03 %). and amplified the number of grains per spike, harvest index, ten kernel weight, and yield/plant, despite of pathogenic interference. Concomitantly, Ag-CNPs nano-priming enhances the harvested seed quality raised from pathogen inoculated seeds plants. The integrated density of the bands obtained in SDS-PAGE of variedly primed seeds revealed that Ag-CNP priming improved wheat's total protein profiling with a denser band intensity. The antioxidant enzymatic activity of CAT, POD, SOD, and NOX was traced maximum in Ag-CNPs primed seeds by 13.52, 7.90, 26.6 and 16.08 units, respectively. Thus, it can be concluded that nano priming with Ag-CNPs mitigated the wheat seeds from pathogenic stress and efficiently increased yield parameters and quality of harvested seeds.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100116"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}