Plant Nano Biology最新文献

{"title":"Nanoparticle-facilitated targeted nutrient delivery in plants: Breakthroughs and mechanistic insights","authors":"Meena Yadav","doi":"10.1016/j.plana.2025.100156","DOIUrl":"10.1016/j.plana.2025.100156","url":null,"abstract":"<div><div>Nanofertilizers (NFs) possess unique properties such as enhanced adhesion to minimize nutrient loss, and slow and controlled nutrient release, that not only enhance plant growth and increase yield but also enable them to ameliorate stress conditions. These properties have been instrumental in addressing the drawbacks of conventional fertilizers. While NFs began to be synthesized in early 2000s, emphasis was laid on synthesis of inorganic NFs during 2010–2020. In the past decade, there have been advancements in the methods of NF synthesis, such as synthesis of metal and metal oxide NFs; synthesis of nano-composites using hydroxyapatite (HA), silica, zeolite and graphene oxide; loading of nutrients onto layered double hydroxides, and chitosan; synthesis of chelated NFs; synthesis of 2-D nano-formulations and 2-D NFs; synthesis of organic NFs using biopolymers and agricultural waste; and synthesis of nano-biofertilizers. This review primarily explores these recent advancements for targeted nutrient delivery within plants and their mechanisms of action, potential of NFs to ameliorate stress such as drought, salinity and metal toxicity to improve crop yield, and factors affecting NFs facilitated nutrient delivery. An integrated approach that uses conventional fertilizers along with NFs, tailor-made for different soil types, and use of artificial intelligence to regulate the release of fertilizers would be an ideal approach to improve plant health and increase yield, with minimal environmental impact. This review provides insights and directions for future research to address plant nutrition under stress and nutrient deficiency conditions.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"12 ","pages":"Article 100156"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging applications of nano-metal-organic frameworks for sustainable agriculture: A critical review","authors":"Renata Carolina Alves ,&nbsp;Vinicius Cagnoto Luna ,&nbsp;Cristiano José Baco ,&nbsp;Ailton José Terezo ,&nbsp;Marilza Castilho ,&nbsp;Adriano Buzutti Siqueira","doi":"10.1016/j.plana.2025.100159","DOIUrl":"10.1016/j.plana.2025.100159","url":null,"abstract":"<div><div>Micro/Nano-organic frameworks (nMOFs) emerge as transformative materials in sustainable agriculture, mitigating the excessive use of agrochemicals that cause environmental degradation and agricultural productivity problems. The high porosity, tunable surface chemistry, and structural adaptability of nMOFs make them ideal for soil applications in agrochemical delivery, remediation, and environmental monitoring. This review examines the unique properties and applications of nMOFs, highlighting their roles in controlled-release agrochemicals, efficient pesticide adsorption, and pesticide sensing. nMOF-based systems enable precise delivery mechanisms responsive to environmental stimuli, reducing chemical waste and environmental contamination while enhancing crop yields. Furthermore, advanced nMOF composites have shown promise in remediating persistent pesticides and detecting agrochemical residues with high sensitivity and selectivity. Despite significant progress, challenges such as large-scale synthesis, cost reduction, and field validation remain. Addressing these limitations can unlock the potential of nMOFs, positioning them as pivotal technologies in the transition toward sustainable agricultural practices. This critical review consolidates current advancements and identifies future opportunities for nMOFs to transform the agricultural sector.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"12 ","pages":"Article 100159"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviating the harmful effect of salinity on faba bean plants using selenium nanoparticles","authors":"Nabil I Elsheery ,&nbsp;Asmaa M Nosier ,&nbsp;Hanfy F Maswada ,&nbsp;Islam I Teiba ,&nbsp;Mohamed Elhamahmy ,&nbsp;Eman M Abdelrazik ,&nbsp;Rehab Abo Ismaeil ,&nbsp;Hala G El-Araby ,&nbsp;Gan Yi ,&nbsp;Libei Li ,&nbsp;Anshu Rastogi","doi":"10.1016/j.plana.2025.100158","DOIUrl":"10.1016/j.plana.2025.100158","url":null,"abstract":"<div><div>Among different abiotic stress salinity is a key factor limiting the growth and productivity of faba bean (<em>Vicia faba</em> L.) plants. In recent years, nanotechnology has been applied to mitigate the harmful effects of salt stress on various plant species. Nanoparticles are compounds with one or more dimensions between 1 and 100 nanometers and differs from their bulk material counterparts. Previous studies have shown that nanoparticles including Selenium can be applied to mitigate the harmful effects on various plant species. Therefore, the present study aims to evaluate the efficacy of Selenium nanoparticles (SeNPs) in mitigating salinity stress in faba bean. To investigate this, we exposed faba beans plants with three different salt concentrations (0 mM, 40 mM, and 80 mM of NaCl) then treated it with foliar sprays of SeNPs at three varying concentrations (0 ppm, 5 ppm, and 10 ppm). Under salinity stress the faba beans plants were observed to be significantly impaired in plant growth and photosynthetic activity. Furthermore, increased levels of malondialdehyde (MDA) in faba beans exposed to salinity indicated significant cellular damage. However, the application of SeNPs under salinity conditions enhanced plant height and chlorophyll content compared to the control. SeNPs also boosted the activity of key antioxidant defense enzymes, including catalase (CAT), ascorbic peroxidase (APX), and polyphenol oxidase (PPO). In addition, they decreased MDA levels and increased proline and phenol concentrations. These findings suggest that SeNPs improved plant health and vitality by mitigating oxidative stress caused by salt. In conclusion, applying SeNPs spray effectively reduced the harmful effects of salt stress on faba bean plants.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"12 ","pages":"Article 100158"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of phenylalanine ammonium lyase gene family in Datura stramonium and expression analysis in response to nanoparticles-aluminium oxide (Al2O3NPs) and tungsten oxide (WO3NPs)","authors":"Baan Munim Twaij ,&nbsp;Hashim K. Mohammed Al-Aubaidi ,&nbsp;Md. Nazmul Hasan","doi":"10.1016/j.plana.2025.100151","DOIUrl":"10.1016/j.plana.2025.100151","url":null,"abstract":"<div><div>The phenylalanine ammonium lyase (PAL) gene family is essential for secondary metabolite production, an important component of plant defense mechanisms. This study characterized the PAL gene family in an important medicinal plant <em>Datura stramonium</em>. Bioinformatics tools were used to identify and analyze PAL gene’s characteristics like phylogenetic relationships, subcellular localization, conserved domains and motifs, cis-elements, and protein structure. In addition, we examined gene expression in response to aluminum oxide nanoparticles (Al₂O₃NPs) and tungsten oxide particles (WO₃NPs) nanoparticles, which are known to induce stress responses in plants. Twelve PAL genes were identified (<em>DsPAL1 - DsPAL12</em>), with protein lengths ranging from amino acids 108–446. These proteins are located in different cellular regions, suggesting different functions. Motif and cis-regulatory element analysis revealed conserved patterns and responses to stress and hormonal signals. Protein-protein interactions suggest that DsPALs play an important role in plant metabolism and secondary metabolite biosynthesis. RT-qPCR data analysis indicated the differential expression of specific PAL genes in response to nanoparticles, indicating their role in secondary metabolite production. Analysis of secondary metabolite production in nanoparticle-treated samples supports a role for PAL genes in secondary metabolite biosynthesis. The characterization of the PAL gene family in <em>D. stramonium</em> has important insights into plant defense mechanisms and secondary metabolite production. This study suggests that the nanoparticles can be used to enhance secondary metabolite production in <em>D. stramonium</em> and provides the basis for future research on plant metabolic production.</div><div>Key message</div><div>PAL gene expression and secondary metabolites production analysis in response to Al₂O₃NPs and WO₃NPs indicates their potential role in secondary metabolites biosynthesis pathways.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"12 ","pages":"Article 100151"},"PeriodicalIF":0.0,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigating plant viruses through nanoparticles: Mechanisms, applications and future perspectives","authors":"R. Rajeshkumar ,&nbsp;Pon Sathya Moorthy ,&nbsp;M. Raveendran ,&nbsp;G. Karthikeyan ,&nbsp;V. Gomathi ,&nbsp;M. Djanaguiraman ,&nbsp;S.K. Rajkishore ,&nbsp;K. Abinaya","doi":"10.1016/j.plana.2025.100155","DOIUrl":"10.1016/j.plana.2025.100155","url":null,"abstract":"<div><div>Understanding the plant virus mechanism plays a major role in crop protection, production and inturn global food security. To develop an effective ways to protect plants from viruses, it is inevitable to understand these interactions throughly. In recent years, nanoparticles are employed as a tool for mitigation of viral infections in plants. This paper presents the role of both metal and non-metal nanoparticles a potential as antiviral agents, focusing on how they interact with both plants and viruses. For example, metal nanoparticles like silver and gold have been found to hinder the virus transmission directly with them. Non-metal nanoparticles, such as chitosan and carbon-based materials, are also showing potential for protecting plants and fighting viruses. The study discuss how nanoparticles are used to deliver treatments directly to the virus by silencing the viral genes. Nanoparticles are even used in laboratory nano-biosensors that can detect plant viruses by offering novel ways to monitoring and diagnosing infections. In addition, the paper explains how the nanoparticles help the plants to boost their defense mechanism by stimulating plant hormones in the production of specific proteins that fight against the viruses. Finally, the study discusses how specialized plant compounds on conjugation when paired with suitable nanoparticles, in enhancing the plant’s ability to resist against viruses. Altogether, these findings suggest that nanotechnology offers a sustainable and versatile way to manage viral diseases in plants, by combining direct antiviral action, gene silencing, early detection, and enhanced plant defenses.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"12 ","pages":"Article 100155"},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of Zn NPs on some growth, biochemical and anatomical factors of chickpea plant stem under UVB irradiation","authors":"Samira Safshekan,&nbsp;Latifeh Pourakbar,&nbsp;Fatemeh Rahmani","doi":"10.1016/j.plana.2025.100154","DOIUrl":"10.1016/j.plana.2025.100154","url":null,"abstract":"<div><div>This study explored the role of zinc oxide nanoparticles (ZnO NPs) in mitigating UV-B radiation effects on chickpea (<em>Cicer arietinum</em> L.) plants. Chickpea plants were grown hydroponically for 45 days and exposed to UV-B radiation for 30 minutes daily over 15 days after reaching the six-leaf stage. ZnO NPs were applied at 50 mg/L and 100 mg/L before UV-B exposure. UV-B reduced root length (40 %), shoot fresh weight (17 %), shoot dry weight (15 %), stem thickness (39 %), and pith parenchyma thickness (5 %), while increasing root fresh weight (59 %), root dry weight (12 %), total phenolic content (TPC, 185.77 %), total flavonoid content (TFC, 94.44 %), and DPPH free radical scavenging activity (31.94 %). ZnO NPs (50 mg/L and 100 mg/L) increased root length (15 % and 25 %), shoot length (21 % and 12 %), shoot fresh weight (56 % and 63 %), and shoot dry weight (40 % and 79 %), respectively. TPC increased by 8 % with 50 mg/L ZnO NPs, while TFC rose by 30 % with 100 mg/L ZnO NPs. DPPH activity improved by 15.78 % and 3.59 % at 50 mg/L and 100 mg/L ZnO NPs, respectively. Stem thickness increased by 12 % and 31 %, and pith thickness by 18 % with 50 mg/L ZnO NPs. UV-B reduced sclerenchyma, xylem, and epidermal cell thickness but increased xylem cell length and pith parenchyma, xylem, and epidermal cell width. Application of 100 mg/L ZnO NPs enhanced xylem thickness, phloem vessel thickness, vascular tissue thickness, and collenchyma tissue thickness. Overall, ZnO NPs demonstrated significant potential in alleviating UV-B-induced damage and improving chickpea plant growth and anatomical integrity.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"12 ","pages":"Article 100154"},"PeriodicalIF":0.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photocatalytic and antimicrobial properties of Boerhavia diffusa bio-callus synthesized Silver nanoparticles","authors":"Wudali Narasimha Sudheer,&nbsp;Juhi Puthukulangara Jaison,&nbsp;Praveen Nagella ,&nbsp;Kadanthottu Sebastian Joseph","doi":"10.1016/j.plana.2025.100153","DOIUrl":"10.1016/j.plana.2025.100153","url":null,"abstract":"<div><div>Plant tissue culture plays a pivotal role in plant biotechnology, and offers innovative and reliable avenues for synthesizing nanoparticles. The approach is safe, replicable, and efficient for therapeutic and environmental sustainability. Despite the proven efficiency of green synthesis approaches, plant callus extracts for nanoparticle synthesis remain moderately investigated. The current study bridges the gap by synthesizing ecofriendly silver nanoparticles (Ag-NPs) using callus extracts of <em>Boerhavia diffusa</em> (Punarnava), an important medicinal plant with proven potential pharmacological properties. These synthesized <em>Boerhavia diffusa-</em>mediated Ag-NPs (BD-Ag-NPs) were characterized using UV-Vis spectroscopy, SEM, FTIR, and XRD. Spectral analysis showed spherical-shaped BD-Ag-NPs with an average size of 9 nm at wavelength 420 nm. Energy-dispersive X-ray (EDX) analysis revealed that silver ions constituted 51.78 % of the total weight of the nanoparticle solutions, while the crystalline structure of the BD-Ag-NPs was confirmed through XRD. Phytoconstituents present in the callus were utilized for capping and the reduction of Ag ions to Ag-NPs was confirmed through FTIR analysis. In addition, BD-Ag-NPs exhibited functional properties like textile dye degradation and broad-spectrum antimicrobial activities against bacterial and fungal pathogens. The current study highlights the potential of employing callus-derived nanoparticles for sustainable environment and biomedical applications. This study advances the application of green nanoparticle synthesis using tissue culture systems and makes significant contributions to addressing global challenges.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"12 ","pages":"Article 100153"},"PeriodicalIF":0.0,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesized MgO-Fe2O3 hybrid nanoparticles for glyphosate herbicide removal and effect on seeds growth characteristics","authors":"Sradhanjali Raut ,&nbsp;Abhilipsa Jena ,&nbsp;Puspita Rout ,&nbsp;Subhalaxmi Jena ,&nbsp;Padmini Nanda ,&nbsp;Lipsa Kumari Dash ,&nbsp;Madhusmita Pattnaik ,&nbsp;Satyabrata Nanda ,&nbsp;Gagan Kumar Panigrahi ,&nbsp;Shraban Kumar Sahoo","doi":"10.1016/j.plana.2025.100152","DOIUrl":"10.1016/j.plana.2025.100152","url":null,"abstract":"<div><div>The increasing prevalence of glyphosate herbicide contamination in water sources and its adverse effects on ecosystems necessitate the development of efficient remediation strategies. In this study, the potential of green-synthesized MgO-Fe₂O₃ hybrid nanoparticles for glyphosate herbicide removal and their effect on seed growth characteristics are investigated. Neem leaf extract was used in the environmentally friendly synthesis of the MgO-Fe₂O₃ nanoparticles, and XRD, BET, FESEM, and TEM studies were used to describe their physicochemical characteristics. The effectiveness of the nanoparticles for removing glyphosate from water was assessed by batch adsorption studies. The adsorption process was examined in relation to a number of parameters, including contact time, dose, pH, and glyphosate concentration. According to the data, at pH= 6, MgO-Fe₂O₃ nanoparticles had a maximum adsorption ability of 111.71 mg/g, demonstrating excellent efficacy in eliminating glyphosate from contaminated water. To comprehend the adsorption mechanism, various adsorption kinetics and isotherms models were examined. Additionally, to evaluate the effect of nanoparticles on seed growth parameters, assays for seed germination and growth were conducted. Moreover, the nanoparticles positively influenced seed growth, root and shoot length, and overall seedling growth characteristics. These findings suggest that green-synthesized MgO-Fe₂O₃ hybrid nanoparticles hold promise for both environmental remediation and agricultural applications.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"12 ","pages":"Article 100152"},"PeriodicalIF":0.0,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143839625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of silver nanoparticles using marigold flowers extract for eco-friendly management of root knot nematode","authors":"Deepika ,&nbsp;Ravi Kant Singh ,&nbsp;Aparna Priyadarshini ,&nbsp;Debia Angel Yeam ,&nbsp;Anjali ,&nbsp;Arpita Bhattacharya ,&nbsp;Richa Vaishnav ,&nbsp;Archna Kumar","doi":"10.1016/j.plana.2025.100150","DOIUrl":"10.1016/j.plana.2025.100150","url":null,"abstract":"<div><div>Plant-parasitic nematodes (PPNs) pose a major threat to global food security, resulting in significant yield loss in economically valuable crops. The growing demand for sustainable and eco-friendly alternatives has driven enthusiasm for plant-based solutions as effective and environmentally responsible approaches. So, in this study, the nematicidal activity of four plant extracts <em>viz</em>., garlic and giloy leaves, giloy stem and marigold flowers were assessed, and it was confirmed that marigold flowers demonstrated the highest phyto-nematicidal properties. We also investigated a sustainable method to enhance the efficacy of phyto- nematicides by fabricating silver nanoparticles (AgNPs) from marigold flowers extract and these green synthesized nanoparticles were characterized by using FTIR, TEM, DLS, Zeta potential and UV–VIS spectrophotometer. In vitro, bioassays were conducted to analyze the impact of marigold aqueous extract and their nanoformulation against <em>Meloidogyne incognita</em> at different time intervals<em>, i.e.</em> 24 h, 48 h and 72 h. Marigold-mediated AgNPs showed the maximum mortality (93.60 %) of <em>M. incognita</em> after 72 h of treatment as compared to the control (6.89 %). The nanoformulation of marigold flowers introduces a novel approach that enhances PPNs suppression while maintaining environmental sustainability. The marigold flowers-mediated bio-nematicide nanoformulation could be used as an ancillary for minimizing the consumption of chemical pesticides in crops. Thus, the marigold-based nematode management strategy may lead to improved crop yield in eco-friendly and cost-effective manner for the achieving of a bio-circular economy.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"12 ","pages":"Article 100150"},"PeriodicalIF":0.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143842523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the protective role of silicon dioxide nanoparticles against copper-induced oxidative damage in soybean plants through altered proline metabolism and antioxidants","authors":"Mohammad Yusuf,&nbsp;Tanveer Alam Khan,&nbsp;Fatima Saif AlBlooshi,&nbsp;Alia Alharmoudi,&nbsp;Meera Saeed AlAlawi,&nbsp;Asma Mohammed Alshkeili,&nbsp;Amna Albedwawi,&nbsp;Jonna Masih,&nbsp;Mahra M. Buty Alghfeli","doi":"10.1016/j.plana.2025.100149","DOIUrl":"10.1016/j.plana.2025.100149","url":null,"abstract":"<div><div>Copper (Cu) is an essential micronutrient for plant growth and development. However, excessive Cu from diverse anthropogenic sources leads to the significant loss of crop production. On the other hand, low dose of silicon dioxide nanoparticles (SiO₂-NPs) showed promising response to various abiotic stresses. This study was conducted to explore the response of SiO₂-NPs in soybean plants exposed to elevated levels of Cu and uncover the efficacy of SiO₂-NPs to alleviate the Cu induced toxicity by regulating proline metabolism and antioxidant system. A low level (25 μM) of Cu in the presence of SiO₂-NPs (50 ppm) significantly increased growth performance, photosynthetic efficiency, and altered proline metabolism. However, higher levels (50, and 100 μM) of Cu alone showed inhibitory effects in concentration dependent manner through increased oxidative stress (electrolyte leakage, H₂O₂ content, and lipid peroxidation). The follow-up application of SiO₂-NPs to the Cu-stressed plants improved growth, water relations, photosynthesis, decreased leaf Cu accumulation and simultaneously enhanced the various antioxidant enzymes viz. catalase, peroxidase and superoxide dismutase with the improved RuBisCO activity and excess proline accumulation along with altered ProDH and P5CS activities. These findings suggested that SiO₂-NPs played a dual role through improved growth and photosynthetic performance of soybean plants in the presence of low dose of Cu and concurrently provide resilience to the soybean plants under higher concentrations of Cu through altered proline metabolism and elevated levels of antioxidant enzymes and proline accumulation resulting in improved growth, water relations and photosynthesis.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"12 ","pages":"Article 100149"},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}