Plant Nano Biology最新文献

{"title":"Nanostructure and plant uptake: Assessing the ecological footprint and root-to-leaf dynamics","authors":"Shadma Afzal ,&nbsp;Nand Kumar Singh ,&nbsp;Arnica F Lal ,&nbsp;Saima Sohrab ,&nbsp;Nivedita Singh ,&nbsp;Pushpraj S. Gupta ,&nbsp;Sanjay Kumar Mishra ,&nbsp;Muhammad Adeel ,&nbsp;Mohammad Faizan","doi":"10.1016/j.plana.2024.100122","DOIUrl":"10.1016/j.plana.2024.100122","url":null,"abstract":"<div><div>Nanostructure design is presented as one of the economically viable technical alternatives for increasing the efficiency of agrochemical use (fertilizers and pesticides) by reducing runoff, increasing foliar uptake and bioavailability, and reducing environmental impact. Nanomaterials (NMs) possess unique properties due to their nanoscale dimensions, typically ranging from 1 to 100 nanometers. At low concentrations, NMs can promote plant growth and development, but at higher doses, they may become toxic, causing oxidative stress, membrane damage, and disrupting key physiological processes. This review aims to comprehensively explore how this toxicity is influenced by NMs properties like chemical composition, dosage, surface structure, and solubility. Gaps in knowledge regarding NMs transport across the root surface and within plants hinder the rational design of NMs for targeted applications. Therefore, this review delves into the physical criteria that affect NMs uptake, translocation, and absorption in plants, as well as the interaction of NMs with plant cells, soil, and their environmental impact. Existing literature on NMs deposited on roots and foliar uptake mechanisms (via stomata, cuticle, trichomes, and necrotic patches) are also examined. The review also discusses how NMs penetrate plant cell walls and utilize plasmodesmata (PD) for translocation between cells, shedding light on the mechanisms and factors influencing these processes. The current knowledge highlights the participation of the symplast, including the PD, in the movement of NMs within the plant. These findings enhance understanding of how plant structure and NM characteristics influence their transport and distribution, aiding the rational design of NMs for controlled uptake and safe application in plants.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100122"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untying the antimicrobial and antioxidant potential of silver nanoparticles fabricated from Typhonium trilobatum (L.) Schott","authors":"Sushree Ghosh,&nbsp;Sankar Narayan Sinha","doi":"10.1016/j.plana.2024.100113","DOIUrl":"10.1016/j.plana.2024.100113","url":null,"abstract":"<div><div>Understanding the properties of silver nanoparticles (AgNPs) is noteworthy for the development of novel antimicrobial agents. Here AgNPs were procured through a sustainable, effortless, simple and lucrative process by using natural reducing agents from aqueous leaf extract of <em>Typhonium trilobatum</em> (L.) Schott. The study mainly aims at the determination of antibacterial and antioxidant activity of the bio-fabricated AgNPs. The synthesis of nanoparticles (NPs) was initially confirmed by UV–vis spectroscopy. The AgNPs were spherical with diameter ranges between 30 and 90 nm, negatively charged at −29.6 mV, crystalline in nature and surrounded by different active functional groups as evident by FTIR spectra analysis. The presence of phenolic compounds such as gallic acid and catechin were confirmed through HPLC analysis, providing insights into the bio-reduction mechanism which facilitate the conversion of Ag+ to AgNPs. Antimicrobial properties of the synthesized AgNPs were assessed against four Gram-negative and two Gram-positive bacteria with maximum zone of inhibition against <em>Staphylococcus aureus</em> (20±3.00 mm) and <em>Micrococcus luteus</em> (20±1.73 mm). The antibacterial potential of AgNPs is primarily linked with the increased cell membrane permeability of AgNPs treated bacterial cells (<em>E. coli</em>, <em>S. aureus</em> and <em>M. luteus</em>) as evident by measuring increased conductivity and elevated extracellular DNA concentration due to the disruption of bacterial cell membrane. Synthesized AgNPs exhibited antioxidant properties with IC₅₀ value of 239.50 mg/L in free radical scavenging activity and IC₅₀ value of 213.23 mg/L in superoxide scavenging activity. To the best of our knowledge, this is the earliest report of biosynthesis and physico-chemical characterization of AgNPs using <em>T. trilobatum</em> leaf extract having efficient antioxidant and antibacterial activity against some bacteria. These plant-mediated AgNPs might offer a promising solution in antibiotic resistance—a growing global health threat.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100113"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic blend: Curcumin-loaded silver nanoparticles synthesized from Talaromyces atroroseus pigment for bio evaluation","authors":"R. Aakash,&nbsp;Kavyarathna,&nbsp;Nagananda G S,&nbsp;Kavya T R,&nbsp;Roopa Reddy,&nbsp;K.U. Minchitha,&nbsp;S. Swetha,&nbsp;Sandeep Suryan","doi":"10.1016/j.plana.2024.100120","DOIUrl":"10.1016/j.plana.2024.100120","url":null,"abstract":"<div><div>Metallic silver, particularly in the form of silver nanoparticles (AgNPs), has gained renewed attention as a powerful antimicrobial solution. In the present investigation, AgNP was synthesized using pigments produced by <em>Talaromyces atroroseus</em>, and Curcumin was loaded onto these AgNP to evaluate their potent antimicrobial, anti-inflammatory, antioxidant and anticancer activities. The maximal biosynthesis of silver nanoparticles (AgNP) was achieved at an optimized precursor concentration of 6 mM silver nitrate (AgNO₃) and a reducing agent concentration of 0.5 g/L fungal pigment. The incorporation efficiency of curcumin onto AgNP was determined to be 11 %. Comprehensive characterization of both curcumin-loaded and unloaded AgNPs was performed using EDS, SEM, FTIR and XRD. Antibacterial assays revealed that both formulations exhibited antimicrobial properties, with the curcumin-loaded AgNPs displaying significantly enhanced inhibitory effects, particularly against <em>Staphylococcus aureus</em>, showing an inhibition zone of 16 ± 0.33 mm. Furthermore, curcumin-loaded AgNPs demonstrated moderate antioxidant activity, with a 36.32 % free radical scavenging rate and an IC₅₀ of 71.9 µg mL⁻¹. The nanoparticles also exhibited potent anti-inflammatory properties, achieving 84.05 % inhibition of inflammation and an IC₅₀ of 96.69 µg mL⁻¹. Notably, curcumin-loaded AgNPs demonstrated cytotoxicity in 100 µg mL⁻¹ of the concentration tested on lung cancer cell line (A549) at 96 hrs. These results suggest that the extended incubation hours or increasing the concentrations of the AgNPs could be lethal concentrations that could completely inhibit the proliferation of lung cancer cells. However, further validation in <em>in-vivo</em> models for toxicity and clearance of AgNPs from the system has to be studied. The observed synergistic effects of biosynthesized curcumin-loaded AgNPs suggest a promising alternative to conventional antibiotic therapies.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100120"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorus transport modeling in soils treated with nano-phosphorus fertilizers","authors":"Kartik Jadav,&nbsp;Maheshwar Durgam,&nbsp;Monisha Perli,&nbsp;Damodhara Rao Mailapalli","doi":"10.1016/j.plana.2024.100112","DOIUrl":"10.1016/j.plana.2024.100112","url":null,"abstract":"<div><div>Nanofertilizers play a promising role in crop production by reducing the application amount and increasing the application efficiency. Understanding the transport of plant nutrients in the soil is crucial for effectively managing nanofertilizer applications and minimizing their impact on the environment. This study investigated phosphorus transport in agricultural soil using 15-day and 30-day soil column experiment data from 2017 and 2018. Two soil column experiments were conducted using nano-rock phosphate, nano-hydroxyapatite fertilizers, and a commercial fertilizer (single super phosphate). The Hydrus 1D model was used to understand the transport mechanism of nano and bulk phosphorus fertilizers. Water and dissolved phosphorus fluxes were simulated using leachate data, and key phosphorus transport parameters, including longitudinal dispersivity and the diffusion coefficient, were determined. The Hydrus-1D model accurately captured leachate dynamics (R² = 0.82–0.99 and MAE = 0.38–0.56 cm/days). Phosphorus transport performed well for bulk fertilizer treatments (R² = 0.86–0.90, MAE = 0.08–0.19 ppm, and RMSE = 0.14–0.36 ppm). However, mixed results were obtained while validating nano fertilizer treatments (R² = 0.31–0.98, MAE = 0.046–0.41 ppm, and RMSE = 0.084–0.35 ppm). For nanofertilizers, the longitudinal dispersivity and distribution coefficient were reduced by 80.71 % and 19.20 %, respectively, compared to commercial fertilizers. The lower longitudinal dispersivity indicates that nano fertilizers release nutrients more slowly than bulk fertilizers. Similarly, a smaller distribution coefficient suggests that nano-phosphorus fertilizers are more concentrated in specific areas within the soil, leading to slower and more controlled nutrient distribution. Additionally, the leachate's observed total phosphorus concentration and the soil profile's phosphorus concentration support the study findings. The results indicate that the transport mechanism of nano and bulk fertilizers in soil is distinct and should be treated separately. This study's findings will contribute to developing optimal fertilizer application strategies for nano-phosphorus fertilizers.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100112"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigate growth of Paris polyphylla under synergic effects of CeO2 and SiO2 using as fertilizers","authors":"Mai Hung Thanh Tung ,&nbsp;Phan Phuoc Minh Hiep ,&nbsp;Nguyen Thi Lieu ,&nbsp;Phan Thi Dieu ,&nbsp;Vo Thi Trong Hoa ,&nbsp;Ajit Sharmaa ,&nbsp;Nguyen Thi Bich Huong ,&nbsp;Do Thi Diem Thuy ,&nbsp;Nguyen Thi Phuong Le Chi","doi":"10.1016/j.plana.2024.100106","DOIUrl":"10.1016/j.plana.2024.100106","url":null,"abstract":"<div><div>This study successfully synthesized SiO₂ and CeO₂ nano-materials to fertilize for <em>Paris polyphylla (P. polyphylla)</em>. The obtained results indicated that nano CeO₂ and SiO₂ enhanced root growth and plan height of the <em>P. polyphylla</em>, respectively. This was due to the <em>P. polyphylla</em> absorbed SiO₂ nano particles via roots and transferred them to the epidermis walls and vascular bundle of stem and leaves to protect as well as to induce growth of aboveground parts while the <em>P. polyphylla</em> also absorbed CeO₂ nanoparticles and retained them in the epidermal roots to provide a medium culture accelerating the nutrient uptake of roots to significantly improve its growth. The simultaneously use of nano CeO₂ and SiO₂ greatly induced both root growth and plan height of the <em>P. polyphylla</em>. The extraction experiments suggested that significant amounts of gracillin, an important medicinal compound, accumulated in the <em>P. polyphylla</em> rhizome. Gracillin content in the rhizome of the CeO₂ fertilized <em>P. polyphylla</em> was also greatly higher than that in the SiO₂ fertilized <em>P. polyphylla</em>. Thus, the nano CeO₂ not only promoted the development but also enhanced formation of gracillin in the rhizome of the <em>P. polyphylla</em>.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100106"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142579116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of agro-waste–derived potassium-doped graphene oxide for antimicrobial activity","authors":"Kamal Garwal ,&nbsp;Chetna Tewari ,&nbsp;Tanuja Arya ,&nbsp;Jyoti Rawat ,&nbsp;Veena Pande ,&nbsp;Souvik Basak ,&nbsp;Mayukh Bose ,&nbsp;Yong Chae Jung ,&nbsp;Nanda Gopal Sahoo","doi":"10.1016/j.plana.2024.100119","DOIUrl":"10.1016/j.plana.2024.100119","url":null,"abstract":"<div><div>Graphene oxide (GO) has been unlocked as a potential bactericidal agent with multifaceted applications for a high degree of abundance of oxidizing functional groups over its structure. The potassium doping of GO (K-GO) enhances its biocompatibility and antimicrobial efficacy. Herein we present a one-step synthesis of K-GO using <em>Quercus ilex</em> (oak fruit) as a sustainable precursor. Characterization via Raman spectroscopy, UV-Vis spectroscopy, TEM, FT-IR, and TGA confirmed the successful synthesis of K-GO. The screening of antimicrobial activity of K-GO was undertaken against Gram-positive (<em>E. faecalis</em> and <em>S. aureus</em>), Gram-negative bacteria (<em>E. coli and P. aeruginosa</em>), and selected fungus (<em>C. albicans</em> and <em>T. asperellum</em>) using the disc diffusion assay. The midpoint inhibitory concentration (IC₅₀) of K-GO against <em>E. coli</em> and <em>S. aureus</em> was found to be 109.64 µg/mL and 38.90 µg/mL, respectively. Employing our green synthesis method using oak seeds as a precursor showcases both cost-effectiveness and sustainability. The aforementioned results suggest that K-GO nano-sheet possesses significant antimicrobial activity, thus may evolve as future antimicrobial nanomaterials.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100119"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcareous soil modified with metallic and organic ZnO nanoparticles limits photosynthetic pigment accumulation and macronutrient uptake in Swiss chard (Beta vulgaris var. cicla)","authors":"Eddaliz García-Reyes ,&nbsp;Josué I. García-López ,&nbsp;Sonia N. Ramírez-Barrón ,&nbsp;Antonio Flores-Naveda ,&nbsp;Perpetuo Álvarez-Vázquez ,&nbsp;Agustín Hernández-Juárez ,&nbsp;Enrique Díaz Barriga-Castro","doi":"10.1016/j.plana.2024.100108","DOIUrl":"10.1016/j.plana.2024.100108","url":null,"abstract":"<div><div>Previous studies on the effects of zinc oxide nanoparticles have mainly examined controlled agricultural settings, failing to consider their behavior in real agricultural soil. As a result, our knowledge of their environmental impact remains incomplete. This study was specifically developed to observe the comparative effects of metallic zinc oxide nanoparticles, zinc sulfate, and zinc oxide green nanoparticles at different concentrations (25, 50, 75 and 100 mg of Zn kg⁻¹ of soil) on growth parameters, the mineral content (N, P, K and Zn) in root and leaf, the content of chlorophyll a (CHLa), b (CHLb), and total (CHLt), and carotenoids in Swiss chard plants grown in calcareous soil. Leaf area and dry root weight increased by 23.27 % and 46.20 %, respectively, in zinc sulfate modified soil. Total chlorophyll and carotenoids also increased by 40.12 % and 32.59 %. The concentration of N, P, K and Zn in roots was 2.89, 1.74, 1.70 and 1.52 times higher, while in leaves, the concentration was 1.48, 1.44, 1.76 and 2.22 times higher in plants grown with zinc sulfate. The effects on plant growth can be attributed to the type of fertilizer used and its influence on macronutrient absorption in the soil. The utilization of zinc sulfate as a soil treatment led to elevated absorption of macronutrients and zinc, suggesting a connection between the fertilizer type and the crop’s agronomic and physiological reactions.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100108"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142651190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant enzymatic activity as an indicator of nano-TiO2 exposure in rice ecosystems","authors":"Raviteja Machanuru ,&nbsp;Manoj Shrivastava ,&nbsp;Renu Singh ,&nbsp;Bhupinder Singh ,&nbsp;Debashis Chakraborty ,&nbsp;Pooja Lakshmidevarahalli Ramalingappa ,&nbsp;Mahesh Narayan","doi":"10.1016/j.plana.2024.100117","DOIUrl":"10.1016/j.plana.2024.100117","url":null,"abstract":"<div><div>The widespread use of nano-titanium dioxide (nano-TiO₂) has raised concerns about its environmental impact, particularly in soil-plant systems. This study investigates the effects of nano-TiO₂ on rice (<em>Oryza sativa</em> cv. PB1121) growth and enzymatic activities, compared to bulk TiO₂, through a pot culture experiment. Eight doses of Ti were applied: six as soil treatments (0, 2.5, 5, 10, 25, and 50 mg Ti kg⁻¹) and two as foliar treatments (0.05 % and 0.1 %). Results showed that grain yield peaked at 25 mg Ti kg⁻¹ soil for both nano and bulk TiO₂, while a 0.05 % foliar spray outperformed by 0.1 %. Titanium accumulated mostly in roots, followed by straw and grains. Nano-TiO₂ significantly increased antioxidant enzyme activities—catalase (CAT), superoxide dismutase (SOD), guaiacol peroxidase, and ascorbate peroxidase (APX)—and lipid peroxidation (measured as malondialdehyde) in rice roots and shoots, indicating oxidative stress. The findings suggest that plant enzymatic activity serves as an early indicator of nano-TiO₂ exposure, making it a valuable biomarker for environmental monitoring. However, higher Ti doses may inhibit plant growth depending on Ti source and concentration. Further studies should examine the effects of nano-TiO₂ of different sizes, shapes, and charges on various crops and soil types to validate these results and assess the broader implications for agricultural and environmental health. This research highlights the dual potential of nano-TiO₂ as both a growth enhancer and a stress-inducing agent, emphasizing the need for careful management in agricultural applications.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100117"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo studies of Vitex negundo-derived silver oxide nanoparticles against Meloidogyne incognita (Root-knot nematode) on tomato plants","authors":"Kanika Choudhary ,&nbsp;Deepak Sharma ,&nbsp;Dixit Sharma ,&nbsp;Pankaj Thakur ,&nbsp;Kushal Thakur ,&nbsp;Sunil Kumar","doi":"10.1016/j.plana.2024.100118","DOIUrl":"10.1016/j.plana.2024.100118","url":null,"abstract":"<div><div><em>Meloidogyne incognita</em> (<em>M. incognita</em>) is an important obligatory pest affecting a large number of horticultural crops. The pest is widely distributed in tropical, subtropical, and warmer regions of the world. <em>M. incognita</em> induces root gall formation that interferes with nutrient supply leading to stunted growth, wilting, chlorosis and significant drop in plant productivity. In recent years biogenic nanoparticles have emerged as an alternative approach for controlling agricultural pests. The current study investigates the nematicidal potential of green-synthesized silver oxide nanoparticles derived from <em>Vitex negundo</em> against second-stage juvenile (J2) and eggs of <em>M. incognita</em>. The maximum mortality (97.20 %) of <em>M. incognita</em> was found after 96 h of treatment. The lowest egg hatching (7.40 %) in comparison with the control (86.93 %) was found in 500 ppm concentration after six days of exposure. The bioassay results revealed that both juveniles and eggs generally showed a concentration-dependent response. The <em>in vivo</em> treatment with biogenic nanoparticles showed reduced gall formation and increased plant growth in tomato plants. Maximum reduction in the galls and plant growth parameters was observed in higher concentrations (1000 ppm) of VND-AgONPs after 60 days compared to untreated inoculated control. To best of our knowledge, this is the first report of the nematicidal efficacy of VND-AgONPs against J2 and eggs of <em>M. incognita.</em> These biogenic nanoparticles could be recommended for managing Root-knot nematodes due to their higher efficacy, cost-effectiveness, and environmentally safe nature.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100118"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of zinc oxide nanoparticles on biosynthesis of thymoquinone in cell cultures of Nigella sativa","authors":"Ambreen ,&nbsp;Mubarak Ali Khan ,&nbsp;Afzal Raza ,&nbsp;Reema Yousaf ,&nbsp;Huma Ali ,&nbsp;Hadeer Darwish","doi":"10.1016/j.plana.2024.100109","DOIUrl":"10.1016/j.plana.2024.100109","url":null,"abstract":"<div><div>The rising market interest for <em>Nigella sativa</em> (Black seeds) necessitates the development of cultivation strategies to enhance metabolites production. Zinc oxide nanoparticles (ZnO-NPs) have drawn global attention as efficient and bio safe elicitors for <em>in vitro</em> cultures, to enhance secondary metabolites production in medicinal plants. In this study, ZnO-NPs were utilized for establishment of callus and cell cultures in black seeds for the first time. Hypocotyl explants were cultured on Murashige and Skoog (MS) media with varying levels of ZnO-NPs, resulted in callus induction and biomass formation. Optimal response in callus growth parameters were observed when explants were grown on MS media supplemented with 60 mg/L ZnO-NPs, resulting in 71.2 % callus induction frequency, 28.2 g/L fresh biomass, 9.7 g/L dry biomass, and 63 % water content. A substantial increase in callus growth was observed when ZnO-NPs were combined with 6-Benzylaminopurine (BA) at ratio of 45:1.5 mg/L, resulting in 91.2 % callus induction frequency and 42.2 g/L fresh biomass. In cell suspension cultures, ZnO-NPs alone at 45 mg/L produced optimum callus biomass (60.9 g/L). However, in combination with BA, callus biomass did not increase significantly in cell cultures. Maximum accumulation of total phenolic content (TPC: 26.8 mg GAE/g DW; Gallic acid equivalent dry weight) and total flavonoid content (TFC: 19.5 mg QE/g DW; Quercetin equivalent dry weight) was observed in cell cultures treated with higher concentration (70 mg/L) of ZnO-NPs in the 5th week of the growth curve. Moreover, ZnO-NPs incremented substantially the Phenylalanine lyase (PAL), Superoxide dismutase (SOD) and Peroxidase (POD) enzyme activities in cell cultures. Nonetheless, Reverse Phase High Performance Liquid Chromatography (RP-HPLC) analysis indicated peak production of thymoquinone (168.5 mg/g FW) in cell cultures treated with 45 mg/L ZnO-NPs alone. This study offers a promising approach for commercial production of <em>Nigella sativa</em> biomass and bioactive metabolites.</div></div>","PeriodicalId":101029,"journal":{"name":"Plant Nano Biology","volume":"10 ","pages":"Article 100109"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}