Pharmacological Research - Modern Chinese Medicine最新文献

{"title":"A mechanistic review on Zanthoxylum species for anti-inflammatory and analgesic potentials","authors":"Acharya Balkrishna ,&nbsp;Meenakshi Panwar ,&nbsp;Sakshi Mishra ,&nbsp;Vidushi Kala ,&nbsp;Vedpriya Arya ,&nbsp;Anurag Dabas","doi":"10.1016/j.prmcm.2024.100553","DOIUrl":"10.1016/j.prmcm.2024.100553","url":null,"abstract":"<div><h3>Introduction</h3><div>In Traditional Chinese Medicine (TCM), <em>Zanthoxylum</em> species are valued for their analgesic and anti-inflammatory effects, relieving pain and controlling inflammation. The study aimed to provide insight into how <em>Zanthoxylum</em> species reduce inflammation and pain by targeting molecular pathways and regulating immune responses.</div></div><div><h3>Methods</h3><div>A structured literature search on PubMed, ScienceDirect, and Google Scholar using targeted keywords yielded a broad selection of sources, which were refined to include 104 articles and 50 books from 1978 to 2023 based on strict criteria. Data extraction focused on identifying bioactive compounds and synthesizing insights into the therapeutic mechanisms of <em>Zanthoxylum</em> species, particularly the key pathways involved.</div></div><div><h3>Results</h3><div>Only 13 of the 33 traditionally used species were shown to be effective in analgesic responses, and only two were employed in treating inflammation ailments. In comparison, 18 species were found to have both anti-inflammatory and analgesic properties. Furthermore, 16 species exhibited anti-inflammatory and analgesic potential in various in-vitro/in-vivo studies. Only ten species studied had anti-inflammatory responses, two had analgesic activity, and four had both anti-inflammatory and analgesic responses. Bioactive compounds like zanthoxylumamides, zanthoaustrones C, tetrahydrobungeanol, decarene, and zanthoxylumamide B exhibit anti-inflammatory effects through MyD88, IRF3, MAPK, and NF-κB pathways. Additionally, the alkaloid benzophenanthridine and essential oils from various plant parts provide analgesic effects by reducing writhing and flinching responses.</div></div><div><h3>Discussion</h3><div>Modern studies support the traditional use of <em>Zanthoxylum</em> species for inflammation and pain, highlighting anti-inflammatory and analgesic effects via key pathways and suggesting further clinical validation for safety and efficacy.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100553"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142748716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding Ganoderma lucidum (Curtis) P. Karst's multipronged potential in improving drug delivery methods and its application in the management of prostate cancer","authors":"Salik Abdullah ,&nbsp;Vivek Bakshi ,&nbsp;Shreya Mukherjee ,&nbsp;Sayandip Paul ,&nbsp;Haider Ali Mollick ,&nbsp;Sk Maksuda ,&nbsp;Biplab Debnath","doi":"10.1016/j.prmcm.2024.100554","DOIUrl":"10.1016/j.prmcm.2024.100554","url":null,"abstract":"<div><h3>Introduction</h3><div>Prostate cancer is the second most prevalent form of cancer in males worldwide, representing a significant global health concern and imposing a substantial health consequence. This type of cancer remains \"localized\" when tumour cells are confined to the prostate or have spread slightly past it (extra-prostatic extension), but have not metastasized to other areas of the body and is considered \"advanced\" when it progresses to other areas of the body.</div></div><div><h3>Methods</h3><div>The present review examines the literature relating <em>Ganoderma lucidum</em> to prostate cancer and the development in nanotechnology-based delivery systems. To relate all three and find a link, a thorough search of literature for more than past 3 decades was done on TCM Bank, TCMSP, PubMed, Google Scholar, ResearchGate, Embase, and ScienceDirect with keywords such as ‘Lingzhi', Traditional Chinese Medicine,<em>G. lucidum</em> polysaccharides, role of TCM in prostate cancer.</div></div><div><h3>Results</h3><div>Conventional cancer treatments might result in significant adverse reactions, making compounds from natural origin a potentially favourable option for cancer therapy. <em>Ganoderma lucidum</em> (<em>G. lucidum</em>), has been extensively utilized in Asian countries for more than two thousand years to promote health and life expectancy. The Shennong's Herbal Classic documented \"Chizhi,\" \"Qingzhi,\" \"Heizhi,\" \"Baizhi,\" \"Huangzhi,\" and \"Zizhi.\" All six varieties of Ganoderma enhance vision, reinforce muscles and bones, bolster renal function, and calm the nerves.Research has established that <em>G. lucidum</em> possesses substantial efficacy in preventing and treating various forms of cancer, including the cancer of the prostate. <em>G. lucidum</em> has demonstrated strong anticancer properties by targeting specific cancer cells, triggering apoptosis, and suppressing tumour growth. Furthermore, formulations utilizing nanotechnology have shown immunomodulatory effects by stimulating the activation of immune cells and improving the body's defence mechanisms.</div></div><div><h3>Conclusion</h3><div><em>G. lucidum</em>, in conjunction with the endless possibilities of nanotechnology, offers a promising frontier in the advancement of innovative therapeutic and biological applications for the treatment of prostate cancer. Additional investigation and advancement in this interdisciplinary domain are necessary to fully harness the combined advantages provided by <em>G. Lucidum</em> and nanotechnology, ultimately resulting in groundbreaking advances in healthcare and well-being.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100554"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143151049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory effect of compound Xuelian capsule on experimental autoimmune myasthenia gravis in mice","authors":"Fukai Gong ,&nbsp;Jianhua Wu ,&nbsp;Min Jiao ,&nbsp;Yalan Ma ,&nbsp;Shilei Zhang ,&nbsp;Qiming Han ,&nbsp;Hongyan Li","doi":"10.1016/j.prmcm.2024.100549","DOIUrl":"10.1016/j.prmcm.2024.100549","url":null,"abstract":"<div><h3>Introduction</h3><div>This study aimed to explore the immunomodulatory effects of Xuelian capsules in experimental autoimmune myasthenia gravis (EAMG) mice.</div></div><div><h3>Methods</h3><div>Female C57BL/6 mice were immunized with AChR-α subunit R97–116 peptide to induce EAMG. Mice were randomized into five groups: EAMG model (<em>n</em> = 8), prednisone (<em>n</em> = 7), and low-, middle-, and high-dose Xuelian capsule groups (<em>n</em> = 7, 7, 8). Hematological parameters and NLR were assessed using an automatic hematology analyzer. Serum AchR-Ab, IFN-γ, IL-4, IL-10 levels were measured by ELISA. Thymus pathology was evaluated by HE staining. Spleen mRNA levels of TGF-β, IL-17, IL-6, IFN-γ were quantified by qPCR, and spleen cell populations by flow cytometry.</div></div><div><h3>Results</h3><div>Xuelian capsules significantly reduced the ratio of NLR in peripheral blood, the concentration of AchR-Ab, IFN-γ, and IL-4 in serum, the expression of TGF-β, IL-17, IL-6, and IFN-γ in the spleen, and the proportion of CD4+/IL-4+ and CD4+/IFN-γ+ cells in the spleen, while increasing the concentration of IL-10 in the serum and the proportion of CD4+/Treg cells in the spleen. It also improved pathological changes in the thymus tissue of mice.</div></div><div><h3>Discussion</h3><div>One of the possible mechanisms of Xuelian capsules in the treatment of myasthenia gravis is to improve the pathological structure of the thymus of EAMG mice by inhibiting inflammatory factors and improving immunity, which then affects the occurrence and development of inflammation to achieve anti-inflammation, thus improving myasthenia gravis (MG).</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100549"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143151050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of nutrients and antioxidant-rich seed oil and sprouted seed oil of Benincasa hispida against formaldehyde-induced hepatic and renal damage","authors":"Md. Boniamin ,&nbsp;Md. Shihab Uddin Sohag ,&nbsp;Md. Saju Ahmad ,&nbsp;Md. Ratul Hasan ,&nbsp;Sabina Yeasmin Sumi ,&nbsp;Quazi Istiaque Bari ,&nbsp;Sanjay Dutta ,&nbsp;Muhammad Abdul Mojid Mondol ,&nbsp;Mst. Jesmin Sultana ,&nbsp;Fazle Rabbi Shakil Ahmed","doi":"10.1016/j.prmcm.2024.100555","DOIUrl":"10.1016/j.prmcm.2024.100555","url":null,"abstract":"<div><h3>Introduction</h3><div>In traditional Chinese medicine (TCM), <em>Benincasa hispida,</em> commonly referred to as wax gourd, winter melon, or ash gourd, has been used extensively for managing fever, urinary dysfunction, cough with viscous mucus, pulmonary and periappendicular abscesses, edema, spermatorrhea, gonorrhea, discharge pus, and expel dampness. In this study, we investigated the antioxidant, macro- and micronutrients as well as the protective effects of <em>Benincasa hispida</em> seed oil (BHSO) and sprouted seed oil (BHSSO) against formaldehyde (FA)-induced liver and kidney damage in mice.</div></div><div><h3>Methods</h3><div>The <em>in vitro</em> antioxidant activity was evaluated using the DPPH radical scavenging assay. Protein, fat, fiber, and total carbohydrate contents were assessed, and micronutrients were determined using flame atomic absorption spectrophotometry. BHSO, BHSSO, and Vitamin E (3485 mg/kg, 3485 mg/kg, and 30 mg/kg p.o., respectively) were administered once daily shortly after formaldehyde exposure (10 mg/kg, I.P.) to separate groups of Swiss albino male mice for a period of 26 days to investigate their protective effects against hepatic and renal damage. The normal control group was given access to sterile tap water and a balanced diet, whereas the disease control group was given only 10 mg/kg of formaldehyde intraperitoneally.</div></div><div><h3>Results and discussion</h3><div>Serum ALT, AST, and creatinine levels were significantly elevated after formaldehyde (FA) administration. Histopathological examinations of liver and kidney tissue revealed degenerative changes in both organs. However, mice pretreated with BHSO and BHSSO showed a significant reduction in FA-induced elevations of serum ALT, AST, and creatinine levels and retained normal liver and kidney tissue histology. BHSO and BHSSO exhibited the ability to scavenge free radicals, as shown <em>in vitro</em> antioxidant studies. Essential micronutrients like zinc (Zn), copper (Cu), calcium (Ca), and chromium (Cr) were also found in both oils. These micronutrients may prevent the inactivation of antioxidant enzymes, thereby increasing free radical scavenging and preserving oxidation and antioxidant equilibrium. Moreover, both oils were found to contain crude fat and carbohydrates that are essential in safeguarding against formaldehyde-induced hepatic and renal damage by offering structural and metabolic support to cells and preserving membrane integrity.</div></div><div><h3>Conclusion</h3><div><em>Benincasa hispida</em> seed oil and sprouted seed oil have significant protective effects against formaldehyde-induced hepatic and renal damage and serve as a valuable source of antioxidants, as well as macro- and micronutrients.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100555"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143150321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the pharmacological mechanism of Tongluo Qingnao formula in treating acute ischemic stroke: A combined approach of network pharmacology, molecular docking and experimental evidences","authors":"Feng Li ,&nbsp;Yilei Dong ,&nbsp;Guangrui Wang ,&nbsp;Zishan Huang ,&nbsp;Wenting Song ,&nbsp;Xiaoyu Zheng ,&nbsp;Peng Zhang ,&nbsp;Mingjiang Yao","doi":"10.1016/j.prmcm.2024.100550","DOIUrl":"10.1016/j.prmcm.2024.100550","url":null,"abstract":"<div><h3>Introduction</h3><div>Tongluo Qingnao (TLQN) Formula is a patent Traditional Chinese Medicine injection designed for the treatment of Acute Ischemic Stroke (AIS) and associated complications. Previous researches had shown promising potential of TLQN in treating ischemic brain edema. This study aimed to reveal specific mechanisms of TLQN in treating AIS by bioinformatics analysis and following confirmatory experiments.</div></div><div><h3>Methods</h3><div>HPLC was used to analyze the active ingredients of TLQN. Network Pharmacology and Molecular Docking were introduced to screen treatment targets and pathways. And then middle cerebral artery occlusion and reperfusion (MCAO/R) model was established in rats to verify the therapeutic effect of TLQN in neurological deficit score, cerebral infarction size, HE staining, TUNEL staining, and proteins expression related to inflammation, apoptosis and the PI3K-AKT signaling pathway.</div></div><div><h3>Results</h3><div>Seven main active ingredients were contained in TLQN. Network pharmacology identified IL-6 and IL-1β as the key targets, and PI3K-AKT as the critical pathway. Molecular Docking analysis indicated that the constituents of TLQN demonstrated a high affinity for crucial targets. TLQN could significantly reduce neurological deficits, pathological lesions and apoptosis caused by AIS. Western blotting results indicated that TLQN can activate the PI3K-AKT signaling pathway, inhibit the expression of pro-apoptotic proteins bax, Caspase-3 and inflammatory factors IL-6, IL-1β.</div></div><div><h3>Discussion</h3><div>TLQN has a therapeutic effect on AIS, and these results highlight the therapeutic effects of TLQN on AIS by activating PI3K-AKT signaling pathway to inhibit inflammation and apoptosis.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100550"},"PeriodicalIF":0.0,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Eriobotrya japonica L. (Chinese medicinal plant) on the regulation of lipid metabolism in atherosclerosis-induced mice and in HepG-2 cells","authors":"Imane Mokhtari ,&nbsp;Dragan Milenkovic ,&nbsp;Souliman Amrani ,&nbsp;Hicham Harnafi","doi":"10.1016/j.prmcm.2024.100552","DOIUrl":"10.1016/j.prmcm.2024.100552","url":null,"abstract":"<div><h3>Background</h3><div>Hyperlipidemia and related metabolic disorders are the main cause of nonalcoholic fatty liver diseases, atherosclerosis and cardiovascular complications. Loquat (<em>Eriobotrya japonica</em>) leaf infusion is an innovative formula derived from Traditional Chinese medicine, known for its therapeutic properties against a number of ailments including inflammation, diabetes and hyperlipidemia.</div></div><div><h3>Aim of the study</h3><div>This study was designed to identify the active compounds of an aqueous extract of <em>E. japonica</em> leaves (ELE) and to investigate its effect on hyperlipidemia and related metabolic complications in high-fat high-sucrose diet-fed mice as well as to elucidate the possible underlying mechanisms.</div></div><div><h3>Materials and Methods</h3><div>Mice were fed a high-fat high-sucrose diet for 3 months and treated with the ELE at doses of 50 mg/kg and 100 mg/kg. Lipids in plasma, liver, adipose tissue, bile and feces were quantified using enzymatic kits. Liver steatosis and oxidative status were highlighted by measuring AST, ALT, ALP, MDA, SOD and catalase activities as well as monitoring of lipid droplets in histological sections. The identification and quantification of possible active compounds were carried out using the HPLC-DAD method. The underlying mechanisms were predicted by <em>in silico</em> study and confirmed by quantifying the expression of the principal involved proteins including PCSK-9, CYP7A1 and p-AMPK in HepG2 cells.</div></div><div><h3>Results</h3><div>The ELE restored lipid metabolism and improved liver histological structures. It also reduced oxidative stress by lowering MDA levels and activating SOD and catalase enzymes. The ELE prevented hepatic steatosis and corrected transaminases profile. HPLC analysis reveals seven phenolic compounds, with ferulic acid being the major one. The extract and its identified phenolic compounds upregulated the expression of CYP7A1 and p-AMPK while downregulated the expression of PCSK-9 in HepG2 cells. The ELE appears to be nontoxic in mice (LD₅₀&gt;5000 mg/kg) and in HepG2 cells at pharmacologically active doses.</div></div><div><h3>Conclusion</h3><div>the ELE could be considered as a source of active biomolecules to produce phytotherapeutics drugs or dietary supplements to treat hyperlipidemia and related cardio-metabolic diseases.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100552"},"PeriodicalIF":0.0,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling biomolecules, antidiabetic and antioxidants properties of DelitesTM via pharmacoinformatics and in vitro investigation","authors":"Juan Leonardo ,&nbsp;Purnawan Pontana Putra ,&nbsp;Trina Ekawati Tallei ,&nbsp;Fatimawali Fatimawali ,&nbsp;Nurpudji Astuti Taslim ,&nbsp;Raymond Rubianto Tjandrawinata ,&nbsp;Vincent Lau ,&nbsp;Saad Mustafa ,&nbsp;Miguel A Prieto ,&nbsp;Fahrul Nurkolis","doi":"10.1016/j.prmcm.2024.100551","DOIUrl":"10.1016/j.prmcm.2024.100551","url":null,"abstract":"<div><h3>Introduction</h3><div>Type 2 diabetes (T2D) remains a global health burden characterized by insulin resistance and chronic hyperglycemia, often exacerbated by oxidative stress, leading to severe complications. Despite the efficacy of pharmacological treatments such as metformin, their side effects and costs highlight the need for alternative therapies. Delites™ (Chong Cao Ling Zhi Xiang Tang), a multi-herbal formulation, presents a promising solution with its bioactive compounds including Apocynin, Curcumin, and Quercetin, which are hypothesized to target T2D pathways.</div></div><div><h3>Methods</h3><div>This study employed pharmacoinformatics, in vitro assays, and molecular dynamics simulations to evaluate the antidiabetic and antioxidative properties of Delites™. Metabolomic profiling using Ultra-High-Performance Liquid Chromatography coupled with High-Resolution Mass Spectrometry identified active compounds, while <em>in silico</em> docking analyzed interactions with T2D-related proteins (<em>e.g</em>., KCNJ11, TCF7L2). Enzyme inhibition assays measured alpha-glucosidase and alpha-amylase activity, and antioxidant potential was assessed using ABTS inhibition.</div></div><div><h3>Results</h3><div>Delites™ demonstrated significant enzyme inhibition (EC₅₀ &lt; metformin), strong binding affinity to T2D proteins (e.g., Sachaliside 2: -9.4 kcal/mol with TCF7L2), and antioxidant activity comparable to Trolox (EC₅₀: 54.44 mg/mL). Molecular dynamics confirmed stable interactions of its compounds with target proteins, while network pharmacology highlighted multi-target potential against diabetes-related pathways.</div></div><div><h3>Discussion</h3><div>The findings underline Delites™ as a multi-target therapeutic candidate for T2D management. Its ability to inhibit carbohydrate-hydrolyzing enzymes, interact strongly with key proteins, and mitigate oxidative stress positions it as a holistic alternative. However, further clinical trials are essential to validate these promising in vitro and <em>in silico</em> results, particularly its long-term efficacy and safety.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100551"},"PeriodicalIF":0.0,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of Bidens pilosa alleviates against alcohol—induced hepatic steatosis in rats: In vivo studies and in silico analysis","authors":"Melva Silitonga ,&nbsp;Hudson Sidabutar ,&nbsp;Hendro Pranoto ,&nbsp;Adriana Yulinda Dumaria LumbanGaol ,&nbsp;Feimmy Ruth Pratiwi Sipahutar","doi":"10.1016/j.prmcm.2024.100546","DOIUrl":"10.1016/j.prmcm.2024.100546","url":null,"abstract":"<div><h3>Background</h3><div>Steatosis has become the critical indicator of the higher alcohol exposure to liver injury. Traditional Chinese medicine, notably <em>Bidens pilosa</em>, is used as anti-inflammatory, antihyperglycemic, and hepatoprotective. This current study aimed to investigate the protective effect of <em>Bidens pilosa</em> leaves ethanolic extract (EEB) against alcohol induced hepatic steatosis.</div></div><div><h3>Methods</h3><div>Using the rat model of alcoholic hepatic steatosis (AHS), this study investigated EEB efficacy through the experimental analysis and in silico method. The animals received EEB at doses of 300 and 600 mg/kg for 42 days in the acute alcoholic experiment. Biochemical analysis and histopathological alteration are examined in alcohol-induced liver rats. Additionally, the pharmacological analysis, molecular docking and dynamic study were evaluated.</div></div><div><h3>Results</h3><div>The results indicated that the EEB has ameliorative effects against hepatic steatosis. It significantly improved hepatic histopathological change, the level of blood glucose, triglyceride, LDL and cholesterol in rats exposed to alcohol. Furthermore, the virtual screening study demonstrated the predominant compounds of EEB might regulate the top core targets linked to AHS, particularly AKT/PIK3CA, MAPK, and MTOR, respectively.</div></div><div><h3>Conclusion</h3><div>Thus, the preventive effect of EEB could be an underlying mechanism for the development of herbal medicine to treat AHS in clinical therapy.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100546"},"PeriodicalIF":0.0,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acorus gramineus extract decreases cancer stem cell properties and stimulates ROS signaling pathway in HepG2 hepatocellular carcinoma cells","authors":"Van Hung Hoang ,&nbsp;Thi Thanh Huong Le ,&nbsp;Phu Hung Nguyen ,&nbsp;Viet Hoang ,&nbsp;Van Khang Pham ,&nbsp;Thi Kieu Oanh Nguyen ,&nbsp;Dinh Quang Hung Can","doi":"10.1016/j.prmcm.2024.100548","DOIUrl":"10.1016/j.prmcm.2024.100548","url":null,"abstract":"<div><h3>Background</h3><div><em>Acorus gramineus</em> is called “Shi Chang Pu'” in Chinese and has been used in traditional Chinese medicine (TCM) for over 2,000 years. The records in the 'Shennong Bencao Jing' show that many species in the genus <em>Acorus</em> are used to treat various diseases in humans such as epilepsy, cardiovascular issues, abdominal pain, memory loss, etc. Several species of the genus <em>Acorus</em> have been demonstrated to exhibit antiproliferative effects against various cancer cell lines. This study aims to evaluate the inhitory effects of <em>Acorus gramineus</em> extract (AGE) on HepG2 liver cancer cells.</div></div><div><h3>Methods</h3><div>The inhibitory effect of AGE on HepG2 cell proliferation was assessed using the MTT assay. Migration was analyzed using the wound healing technique. The 3D culture technique was employed to evaluate tumorsphere formation and the invasive potential of cancer cells. Real-time PCR was used for mRNA expression analysis. Changes in the expression levels of CD44 protein and ROS were analyzed using fluorescence staining techniques. Chemical compounds of AGE were identified using UPLC-QToF-MS.</div></div><div><h3>Results</h3><div>The results indicate that AGE exhibits potent inhibitory activity against cell proliferation, with an IC50 value of 34.1 mg/mL. AGE significantly inhibited both the formation and growth of tumorspheres in 3D cultures, reducing both their number and size. Additionally, AGE was found to suppress the invassion of cancer cells. AGE regulated the downregulation of stem cell-related markers including NANOG, SOX2, OCT4, and ALDH. AGE was also shown to increase the production of reactive oxygen species (ROS) within cells by activating genes involved in ROS signaling pathways, which may lead to a decrease in cell proliferation, migration, and invasion, as well as a reduction in cancer stem cell properties. Finally, 58 compounds present in AGE were identified by UPLC-QtoF-MS analysis, and Mulberrofuran K, Akebonoic acid, and Shancilin were evaluated for their affinity with protein markers related to liver cancer stem cells.</div></div><div><h3>Conclusion</h3><div>This study is the first report on the inhibitory activity of AGE against cancer stem cell characteristics in HepG2 cells, highlighting the potential of this plant species as an anti-liver cancer agent.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100548"},"PeriodicalIF":0.0,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gu Sui Bu (Drynaria fortunei J. Smith) prevents osteoporosis in ovariectomized rats by inhibiting pyroptosis through NLRP3/GSDMD/CASPASE-1","authors":"Hui su ,&nbsp;Jun Dong ,&nbsp;Luyao Liu ,&nbsp;Zechen Yan ,&nbsp;Rujie Zhuang ,&nbsp;Guangxin Huang ,&nbsp;Haipeng Xue ,&nbsp;Zhanwang Xu ,&nbsp;Yu Pan","doi":"10.1016/j.prmcm.2024.100544","DOIUrl":"10.1016/j.prmcm.2024.100544","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Traditional Chinese medicine Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;), has the effect of tonifying the kidneys and strengthening bone. There are many modern studies on the anti-osteoporosis pharmacological mechanism of Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;) but no reports on the pharmacological mechanism of Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;) improving cell pyroptosis and anti-osteoporosis have been found.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Aim&lt;/h3&gt;&lt;div&gt;This study aims to verify the changes in cellular standard indicators in postmenopausal osteoporosis, thereby revealing the participating mechanism of pyroptosis and the intervention effect of Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith)&lt;/em&gt; .&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;) subjected to UHPLC-Q-Orbitrap-MS/MS analysis, and the OVX rat model was constructed in vivo as the research object. It was divided into sham operation group (SHAM), ovariectomized osteoporosis model group (OVX) and Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;) group (TFRD-L, TFRD-H). After 3 months of modeling, the medication group was treated with Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith&lt;/em&gt;) and the samples were collected after 12 weeks of intervention. ELISA was used to detect the levels of Caspase-1, NLRP3, GSDMD, IL-1β, and IL-18 in rat serum; the right femur was taken for Micro-CT large bone microstructure scanning and femoral BMD detection; the femur was subjected to rat histopathology HE, TRAP staining; immunohistochemistry and immunofluorescence staining of rat histopathology were performed. WB and PCR were used to observe the expression of Osteoblasts and pyroptosis-related indicators Caspase-1, NLRP3, GSDMD and RUNX2, IL-1β, and IL-18.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;UHPLC-Q-Orbitrap-MS/MS analysis the main compounds in Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith)&lt;/em&gt; samples were identified. These 9 chemical components are Palmitic acid, Fisetin, Caffeic acid, Naringin, Rutin, Uridine, Cafestol, Astilbin . Rat Micro-CT, The results of HE staining and TRAP showed that compared with the rats in the OVX group, the number of bone trabeculae in the rats in the &lt;em&gt;Gu Sui Bu (Drynaria fortunei J. Smith)&lt;/em&gt; medication group (TFRD-L, TFRD-H) increased, became wider and thicker, and the bone density increased. Continuity increases and bone lacunae decrease. Rat serum ELISA, femoral tissue immunohistochemistry, immunofluorescence staining and WB, PCR showed that compared with the OVX group, Caspase-1, NLRP3, The expression levels of GSDMD and inflammation were reduced (p&lt;0.05), and the expression of osteogenic marker RUNX2 was reduced and increased (p&lt;0.05).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;The traditional Chinese medicine Gu Sui Bu (&lt;em&gt;Drynaria fortunei J. Smith)&lt;/em&gt; can improve the bone density of ovariectomized osteoporosis model rats and significantly enhance the bone microstructure. At the same time, it reduced the expres","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"13 ","pages":"Article 100544"},"PeriodicalIF":0.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}