Pharmacological Research - Modern Chinese Medicine最新文献

{"title":"In-vitro and in-vivo assessment of cytotoxic potential of β-1,3 beta-glucan comprising Lentinan against glioblastoma multiforme","authors":"Sagar Trivedi ,&nbsp;Veena Belgamwar ,&nbsp;Satyendra Prasad","doi":"10.1016/j.prmcm.2024.100568","DOIUrl":"10.1016/j.prmcm.2024.100568","url":null,"abstract":"<div><h3>Introduction</h3><div>Lentinan (LNT), a bioactive β-glucan from Shiitake mushroom (<em>Lentinus edodes</em>), is traditionally used in Chinese medicine for immune support and cancer therapy. This study evaluates LNT's potential to treat glioblastoma multiforme (GBM) by exploring its immune-modulatory, antiangiogenic, cytotoxic, antioxidant, and antiproliferative effects.</div></div><div><h3>Methods</h3><div>The anti-cancer effects of LNT were assessed through <em>in-vitro</em> and <em>in-vivo</em> models. The antiangiogenic activity was measured via the CAM model, cytotoxicity through MTT and SRB assays on U87 MG cells, antioxidant potential by DPPH scavenging, and mitochondrial membrane potential using Rhodamine-123. Antimitotic and antiproliferative activities were also examined.</div></div><div><h3>Results</h3><div>LNT showed significant antiangiogenic activity at 15–20 mg/pellet (<em>p</em> &lt; 0.01) and reduced U87 MG cell viability dose-dependently, with an IC₅₀ of 96.11 ± 0.21 µg/mL at 48 h. It demonstrated 84.21 ± 1.29 % antioxidant scavenging at 100 µg/mL and lowered the mitotic index to 33 ± 1.36 % at 25 mg/mL. Mitochondrial membrane potential (MMP) showed a significant decrease in the green fluorescence (<em>p</em> &lt; 0.01). Antimitotic potential showed maximum inhibition of mitosis of 33 ± 1.36 % at 25 mg/mL along with chromosomal abnormalities on the treated root tips. In yeast cells, LNT showed substantial antiproliferative activity.</div></div><div><h3>Discussion</h3><div>These findings support LNT's traditional use in Chinese medicine and highlight its potential against GBM.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100568"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI drug discovery tools and analysis technology: New methods aid in studying the compatibility of Traditional Chinese Medicine","authors":"Qiwu Jiang ,&nbsp;Suhan Yang ,&nbsp;Shan He ,&nbsp;Fei Li","doi":"10.1016/j.prmcm.2024.100566","DOIUrl":"10.1016/j.prmcm.2024.100566","url":null,"abstract":"<div><h3>Introduction</h3><div>The compatibility of Traditional Chinese Medicine (TCM) holds the potential for reducing toxicity and enhancing efficacy, serving as a crucial guide for the clinical application of TCM. In recent years, the development of artificial intelligence (AI) drug discovery tools has introduced novel approaches for analyzing the multichemical components of TCM, thereby saving time and efforts in experiments.</div></div><div><h3>Methods</h3><div>The keywords \"Traditional Chinese Medicine\" and \"Artificial Intelligence\", \"Traditional Chinese Medicine\" and \"drug compatibility\" were searched across various literature databases, including Web of Science, Google Scholar, PubMed, and Elsevier. Over 100 articles were reviewed, and after narrowing the selection to those focused on compatibility, the chosen studies were carefully analyzed to summarize the latest developments for this review.</div></div><div><h3>Results</h3><div>The review introduce AI drug discovery tools, including virtual screening, target prediction, ADMET prediction, and data mining, along with their roles in studying TCM compatibility. The results further provide insights of AI's application in TCM combination prediction, TCM compatibility mechanisms, and optimization of TCM compatibility ratio within the TCM compatibility research field.</div></div><div><h3>Discussion</h3><div>Traditional Chinese Medicine uses holistic formulas involving multiple components, targets, and pathways for disease treatment, but scientific explanations of these formulas are limited. AI aids TCM research by predicting combinations, mechanisms, and optimizing ratios, which improves efficiency and reducing costs. However, AI predictions may not be definitely accurate, and traditional expertise is still essential for validation. Future applications of AI in TCM require improved tools and collaboration between AI and TCM researchers.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100566"},"PeriodicalIF":0.0,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143138018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated network pharmacology, bioinformatics, and experimental approach to explore the mechanism of honokiol liposomes against glioblastoma","authors":"Fang Cheng ,&nbsp;Yingfang Ma ,&nbsp;Neng Qiu,&nbsp;Yutao Yang,&nbsp;Zhichun Shen,&nbsp;Xintian Lai,&nbsp;Jianhua Ge,&nbsp;Yingjia Guo","doi":"10.1016/j.prmcm.2024.100567","DOIUrl":"10.1016/j.prmcm.2024.100567","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Introduction&lt;/h3&gt;&lt;div&gt;Honokiol (HK), a bioactive compound isolated from &lt;em&gt;Magnolia officinalis Rehd. et Wils.&lt;/em&gt; (commonly called &lt;em&gt;Houpu&lt;/em&gt; Magnolia or Magnolia-bark), has demonstrated significant anticancer potential across various malignancies. Despite its promising therapeutic effects, the clinical application of honokiol has been hampered by its low bioavailability and poor aqueous solubility. To address these limitations, we have investigated the use of liposomal drug delivery systems. Preliminary results from our phase I clinical trials have shown encouraging outcomes: patients with recurrent glioblastoma multiforme (GBM) treated with honokiol liposomes exhibited a longer median overall survival (15 months) compared to those receiving bevacizumab (9.3 months), a standard treatment option. While these initial findings are promising, the precise mechanisms underlying the enhanced efficacy of honokiol when delivered via liposomes remain to be fully elucidated.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;Various databases including TCMSP, CTD, BATMAN-TCM, PharmMapper, and SwissTargetPrediction were searched to identify honokiol targets and GBM targets were obtained from GeneCards, OMIM, and DisGeNET disease databases. The component-disease intersection target protein-protein interaction (PPI) network was constructed using String database and Cytoscape3.9.1. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of major intersection targets was performed using DAVID. Differential expression targets among the major intersection targets were analyzed using GEPIA, HPA, ROC Plotter, and TIMER databases. Finally, molecular simulation docking verification was conducted using Auto Dock4.2. Finally, we conducted targeted experiments to validate and assess key biological processes, molecular functions, and signaling pathways identified through our comprehensive network pharmacology analysis. Our approach included in vitro studies focusing on proliferation, cell cycle progression, and apoptosis, as well as in vivo experiments examining inflammatory responses.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Network pharmacological screening identified 31 major intersecting targets of honokiol and GBM. GO analysis revealed significant enrichment (&lt;em&gt;P&lt;/em&gt; &lt; 0.05) in 316 biological processes, 38 cellular components, and 70 molecular functions. KEGG pathway analysis highlighted significant correlations with cancer pathways, metabolic disease pathways, and inflammatory signaling pathways. Further bioinformatic analysis pinpointed 16 core targets significantly affecting GBM, with molecular docking confirming honokiol's spontaneous binding to these targets. Both in vitro and in vivo experiments substantiated these findings, demonstrating that honokiol and its liposomal formulation effectively suppress GBM cell proliferation, induce cell cycle arrest and apoptosis, while significantly reducing inflammatory cytokine express","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100567"},"PeriodicalIF":0.0,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological relevance of anthocyanin derivative: A review","authors":"Pavithra Bharathy ,&nbsp;Punniyakoti V. Thanikachalam","doi":"10.1016/j.prmcm.2024.100565","DOIUrl":"10.1016/j.prmcm.2024.100565","url":null,"abstract":"<div><h3>Background</h3><div>Anthocyanins, a group of natural flavonoid pigments responsible for red, purple, and blue hues in plants, are valued in Traditional Chinese Medicine (TCM) for their health benefits including antioxidant, anti-inflammatory, and organ-protective effects.</div></div><div><h3>Objective</h3><div>This review explores the pharmacological properties of anthocyanins in relation to TCM applications, focusing on their antioxidant, anti-inflammatory, cardioprotective, neuroprotective, anticancer, metabolic, and skin health effects. Additionally, it highlights the challenges with bioavailability and strategies to enhance absorption.</div></div><div><h3>Methods</h3><div>A systematic literature review was conducted across PubMed, Scopus, and Web of Science, examining studies on anthocyanin chemistry and therapeutic applications aligned with TCM principles.</div></div><div><h3>Results</h3><div>Anthocyanins display structural diversity, which contributes to their wide-ranging bioactivity. Cyanidine, delphinidine and rutinoside compounds serve as potent antioxidants, reduce inflammation, support cardiovascular and neuroprotection, inhibit cancer cell growth, improve metabolic profiles, and protect skin from damage. However, the limited bioavailability of peonidin, pelargonidin, and malvidin hinders their full therapeutic potential.</div></div><div><h3>Discussion</h3><div>This review highlights the diverse therapeutic potential of cyanidine and delphinidine, in alignment with Traditional Chinese Medicine principles to enhance vitality and support healing. Their antioxidant, anti-inflammatory, cardioprotective, neuroprotective, anticancer, and metabolic benefits suggest that they may play a key role in managing chronic diseases. Although challenges in bioavailability remain, strategies such as encapsulation and co-ingestion show promise in improving efficacy. Future research should focus on clarifying the molecular mechanisms, optimizing formulations, and exploring interactions with the gut microbiota to fully realize their therapeutic potential.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100565"},"PeriodicalIF":0.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143138016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TCMNP: A data processing and visualization database and R package for traditional Chinese medicine network pharmacology","authors":"Min Ying ,&nbsp;Jing Feng ,&nbsp;Mei Liu ,&nbsp;Lijuan Wang ,&nbsp;Jinkun Liu","doi":"10.1016/j.prmcm.2024.100562","DOIUrl":"10.1016/j.prmcm.2024.100562","url":null,"abstract":"<div><h3>Background</h3><div>Network pharmacology has become crucial for elucidating the mechanisms underlying traditional Chinese medicine (TCM). However, challenges persist in processing and visualizing complex data related to TCM network pharmacology (TCMNP). To address this, we developed ''TCMNP'', a specialized database and R package within the R environment, aimed at enhancing data processing and visualization capabilities for TCM research.</div></div><div><h3>Methods</h3><div>''TCMNP'' integrates data from prominent databases, including DrugBank, TCMSP, ETCM, DisGeNET, and OMIM, to establish a comprehensive relational framework connecting TCM prescriptions, medicinal materials, bioactive ingredients, therapeutic targets, and associated diseases. Leveraging packages such as ''dplyr'', ''clusterProfiler'', ''ggplot2'', and ''circlize'', we created a suite of functions that facilitate streamlined data analysis and advanced visualizations within R.</div></div><div><h3>Results</h3><div>''TCMNP'' supports comprehensive analysis and visualization workflows for TCM compounds, individual herbs, active ingredients, molecular targets, and biological pathways. The database comprises 571 TCM herbs, 17,118 unique ingredients, 10,013 diseases, and 15,956 targets, enabling automated processes from compound component screening to target and pathway enrichment analysis. Additional features include protein-protein interaction mapping and transcription factor-gene target analysis, complemented by a user-friendly interface for generating TCM network pharmacology visualizations. ''TCMNP'' is accessible at <span><span>https://tcmlab.com.cn/tcmnp</span><svg><path></path></svg></span>, and the package available at <span><span>https://github.com/tcmlab/TCMNP</span><svg><path></path></svg></span>.</div></div><div><h3>Conclusion</h3><div>''TCMNP'' database and package provide user-friendly visualization tools for analyzing the components of TCM formulas and their potential therapeutic effects on diseases. We anticipate that ''TCMNP'' will help to explore the mechanism of TCM to treat diseases.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100562"},"PeriodicalIF":0.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginger (Zingiber officinale) in traditional Chinese medicine: A comprehensive review of its anti-inflammatory properties and clinical applications","authors":"Jitendra Gupta ,&nbsp;Bhawna Sharma ,&nbsp;Rahul Sorout ,&nbsp;Ram Gopal Singh ,&nbsp;Ittishree ,&nbsp;Mukesh Chandra Sharma","doi":"10.1016/j.prmcm.2024.100561","DOIUrl":"10.1016/j.prmcm.2024.100561","url":null,"abstract":"<div><h3>Introduction</h3><div>Traditional Chinese medicine (TCM) has widely used ginger (<em>Zingiber officinale</em>) for its anti-inflammatory properties and various clinical applications. This comprehensive review aims to explore the phytochemical properties of ginger, focusing on its antioxidant components and active ingredients such as shogaols, gingerols, and zingerone. The unique composition of ginger, includes terpenoids, flavonoids, and volatile oils, contributes to its therapeutic potential in managing various health conditions.</div></div><div><h3>Methodology</h3><div>We conducted a thorough examination of existing literature to gather information on the anti-inflammatory properties and clinical applications of ginger on conditions like osteoarthritis, rheumatoid arthritis, irritable bowel syndrome, and asthma to gain insights into its efficacy and mechanisms of action. We also focused on studies reporting on the phytochemical composition of ginger, including its antioxidant compounds and active ingredients, to understand how these components contribute to its therapeutic effects.</div></div><div><h3>Results</h3><div>The review revealed that ginger possesses antioxidant properties attributed to compounds like shogaols, gingerols, and zingerone. Additionally, the terpenoids, flavonoids, and volatile oils found in ginger play a crucial role in its anti-inflammatory and health-promoting effects. Furthermore, studies have shown that supplementing with ginger can improve lung function and mitigate airway inflammation in asthma patients.</div></div><div><h3>Conclusion</h3><div>Ginger emerges as a valuable botanical remedy in TCM due to its diverse phytochemical profile and therapeutic properties. The antioxidant components and active ingredients present in ginger contribute to its anti-inflammatory effects and clinical applications in managing various health conditions. We need to conduct further research and clinical trials to fully explore the potential of ginger for promoting health and well-being.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100561"},"PeriodicalIF":0.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qiangyang decoction attenuates ischemia/reperfusion-induced cardiomyocyte ferroptosis via ULK1/KEAP1/Nrf2 axis","authors":"Jing Wang ,&nbsp;Wan Cai ,&nbsp;Jingxuan Chen ,&nbsp;Wenjin Wang ,&nbsp;Wentao Fu ,&nbsp;Wen Ge ,&nbsp;Hao Chi","doi":"10.1016/j.prmcm.2024.100560","DOIUrl":"10.1016/j.prmcm.2024.100560","url":null,"abstract":"<div><h3>Background</h3><div>Myocardial ischemia-reperfusion (I/R) induced cardiomyocyte death is a major cause of cardiac dysfunction and fibrosis. Ferroptosis of cardiomyocytes has been demonstrated to contribute to I/R induced injury. Qiangyang decoction (QYD) is commonly used in clinical treatment of hypertension and cardiac fibrosis. However, the intervention effect of QYD on I/R injury, especially its therapeutic efficacy on ferroptosis in cardiomyocytes, remains unclear. This study aimed to investigate the effect of QYD against I/R induced cardiomyocyte ferroptosis and to reveal the underlying mechanism.</div></div><div><h3>Methods</h3><div>In this study, UPLC-MS/MS was conducted to identify the component of QYD. An in vivo I/R mouse model and an in vitro cardiomyocytes OGD model were utilized to evaluate the cardiac protective effect of QYD. Transcriptome analysis and western blotting assays were used to explore the mechanism.</div></div><div><h3>Results</h3><div>A high dose of QYD exerts a cardioprotective effect through inhibiting I/R-induced cardiomyocyte ferroptosis. A high dose of QYD ameliorated I/R and OGD injury; these beneficial effects were not further enhanced by the ferroptosis inhibitor Fer-1. Anti-ferroptosis factors FPN1, Homx1, and GPX4 were transcriptionally upregulated by QYD both in mouse heart and adult mouse cardiomyocytes (AMCMs). Consistently, the kelch like ECH associated protein 1 (KEAP1) level and nuclear factor erythroid 2-related factor 2 (Nrf2) degradation were remarkably suppressed. RNA-sequencing results revealed that unc-51 like autophagy activating kinase 1 (ULK1), a serine kinase mediating the autophagy-dependent degradation of KEAP1, was significantly up-regulated in QYD-treated mouse hearts. The ULK1 inhibitor SBI-0206965 abolished the inhibition of QYD on KEAP1 and notably weakened the cardiac protective effect.</div></div><div><h3>Conclusions</h3><div>These findings suggest that QYD exerted cardiac protection by activating ULK1 and subsequently inhibited KEAP1, thereby enhancing Nrf2 level and initiating the transcription of ferroptosis-resistance genes. These findings highlight that QYD may serve as a cardioprotective strategy for I/R.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100560"},"PeriodicalIF":0.0,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jinxinkang granule alleviates chronic heart failure by enhancing GPER/AMPK/PCG-1α-mediated fatty acid oxidation","authors":"Haixiang Zhong ,&nbsp;Liu He ,&nbsp;Wanru Zhong ,&nbsp;Lili Wang ,&nbsp;Jianmin Luo ,&nbsp;Qi Chen ,&nbsp;Rong Li ,&nbsp;Rong Zhang ,&nbsp;Zhongqiu Liu ,&nbsp;Yuanyuan Cheng","doi":"10.1016/j.prmcm.2024.100556","DOIUrl":"10.1016/j.prmcm.2024.100556","url":null,"abstract":"<div><h3>Introduction</h3><div>Chronic heart failure (CHF) is considered to be the last battlefield of cardiovascular disease. Jinxinkang granule (JXK) is an integrated traditional Chinese medicine formula for treating CHF. Our previous clinical study showed that JXK could increase the left ventricular ejection fraction and reduce concomitant symptoms in patients with CHF. However, the molecular mechanism underlying the action of JXK on CHF has yet to be fully illustrated.</div></div><div><h3>Methods</h3><div>Sprague-Dawley (SD) rats under left anterior descending artery ligation model, C57BL/6 mice subcutaneously injected isoproterenol (ISO) model in vivo and H9c2 cells under oxygen glucose deprivation (OGD) in vitro were used to evaluate the effect of JXK against CHF. Cardiac function and morphology assessment using M-mode echocardiography, H&amp;E/Masson/WGA staining, western blotting analysis, real-time PCR detection, ATP and cAMP assays were used to explore the mechanism of JXK against CHF.</div></div><div><h3>Results</h3><div>JXK improved cardiac function and ameliorated cardiac fibrosis in both LAD-induced CHF rats and ISO-induced CHF mice, and protected cardiomyocytes from OGD injury in H9c2 cells. Moreover, JXK increased the level of ATP content and enhanced fatty acid oxidation by increasing CD36, CPT1A and ACADM expression. Mechanism study showed that JXK increased the GPER expression and cAMP level, promoted AMPK phosphorylation and PGC-1α expression. Additionally, molecular docking analysis showed that dihydroisotanshinone I has a high binding affinity with GPER at -8.5 kcal/mol, which has the similar binding sites with G-1, a GPER agonist. Importantly, after using the inhibitor of GPER (G15), the cardioprotective effect of JXK and the enhancing effect on the fatty acid oxidation were blocked.</div></div><div><h3>Conclusion</h3><div>JXK effectively activated GPER/AMPK/PGC-1α signaling pathway to enhance the oxidation of fatty acid, thereby promoting cardiac energy metabolism production to ameliorate CHF.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100556"},"PeriodicalIF":0.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143137975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Traditional Chinese Medicine for myopathy: A comprehensive review of herbal therapeutics","authors":"Aswini Rajendran ,&nbsp;Veronica Elumalai ,&nbsp;Palanirajan Vijayaraj Kumar ,&nbsp;Karthikeyan Elumalai","doi":"10.1016/j.prmcm.2024.100557","DOIUrl":"10.1016/j.prmcm.2024.100557","url":null,"abstract":"<div><h3>Introduction</h3><div>Myopathy, a group of conditions affecting muscles, is characterized by muscle weakness and degeneration, resulting in reduced quality of life. While existing treatments primarily address symptoms, they often fail to target underlying causes and may produce adverse effects. Traditional Chinese Medicine (TCM) offers a promising alternative through its herbal remedies, which aim to enhance muscle function, reduce inflammation, and promote cellular repair.</div></div><div><h3>Methods</h3><div>This review investigates the current literature on Traditional Chinese Medicine (TCM) in the treatment of myopathy. Our analysis focused on particular herbs, such as Astragalus (Huang Qi), Ginseng (Ren Shen), and Angelica sinensis (Dang Gui), as well as herbal combinations like Bu Yang Huan Wu Tang. Through an evaluation of pharmacological research, clinical case studies, and controlled trials, we obtained valuable information regarding the therapeutic mechanisms and clinical efficacy of these TCM approaches.</div></div><div><h3>Results</h3><div>Studies highlight the ability of Traditional Chinese Medicine (TCM) herbs to protect and restore muscles through their anti-inflammatory, antioxidant, and metabolic regulatory properties. Investigations have demonstrated improvements in muscle strength, function, and patient outcomes. TCM showed potential supplementary benefits when integrated with standard medical approaches.</div></div><div><h3>Discussion</h3><div>Traditional Chinese Medicine (TCM) offers a comprehensive strategy for addressing myopathy, showing encouraging outcomes in enhancing muscle function and patient well-being. However, despite its potential benefits, there are still obstacles to overcome regarding consistency, risk management, and scientific verification. Additional studies are required to effectively incorporate TCM into established treatment protocols.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100557"},"PeriodicalIF":0.0,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143138010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotective activity of Alstonia boonei (De Wild) stem bark in isoniazid-induced Wistar rats: Antioxidant, anti-inflammatory and in silico evaluations","authors":"Adedotun F. Adesina ,&nbsp;Joseph T. Apata ,&nbsp;Olusegun O. Babalola ,&nbsp;Chiagoziem A. Otuechere ,&nbsp;Mukaila B. Adekola ,&nbsp;Gbenga S. Ogunleye ,&nbsp;Funke Asaolu","doi":"10.1016/j.prmcm.2024.100558","DOIUrl":"10.1016/j.prmcm.2024.100558","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Introduction&lt;/h3&gt;&lt;div&gt;Liver damage is mainly caused by exposure to toxic chemicals and is a global health challenge. A variety of &lt;em&gt;Alstonia&lt;/em&gt; species including &lt;em&gt;Alstonia boonei&lt;/em&gt; has been used in Traditional Chinese Medicines (TCM) for the treatment of fever, asthma and as analgesics. In this research, the potential of &lt;em&gt;A. boonei&lt;/em&gt; stem bark ethanol extract and ethyl acetate fraction in restoring isoniazid-induced hepatic damage in rats was studied using antioxidative and anti-inflammatory mechanisms.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;&lt;em&gt;A. boonei&lt;/em&gt; stem bark was extracted with ethanol and fractionated with n-hexane, ethyl acetate, and butanol. &lt;em&gt;In vitro&lt;/em&gt; phytochemical screening and quantification, DPPH, ferrous ion chelating and ferric reducing antioxidant activity of the extracts were carried out. Determination of erythrocyte membrane stabilizing and inhibition of protein denaturation activity of the extracts and fractions were used to evaluate anti-inflammatory activity. Gas chromatography- mass spectrometry (GCMS) of the most active fraction was analyzed. A total of 18 and 35 (7 groups of 5) rats were used for both acute and sub-chronic toxicity studies respectively for a period of 60 days. The control group received normal saline, while 15 mg/kg isoniazid (INH) was used to induce liver toxicity. 25, 50 and 100 mg/kg ethyl acetate fractions (EAF), 50 mg/kg of ethanolic extract and 50 mg/kg vitamin C were used as treatment against INH-induced toxicity. The rats were sacrificed and plasma and liver tissues were obtained for biochemical and histological analyses. Plasma markers of hepatic function including alanine aminotransferase (ALT), aspartate amino transferase (AST), lactate dehydrogenase (LDH), gamma-glutamyl transferase (GGT), albumin and bilirubin levels were determined. Activity of Liver enzyme antioxidants including superoxide dismutase (SOD) and catalase were also analyzed. Liver tissues were further subjected to histological analyses. &lt;em&gt;In silico&lt;/em&gt; molecular docking and ADMET studies were used to analyze the binding interactions with arginase 1 and thioredoxin reductase and physicochemical properties of the GC–MS identified compounds.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Phytochemicals including alkaloids, flavonoids and phenols were present in the plant's extract and fractions, while they also demonstrated antioxidant activity against DPPH radicals, and ferrous ion, with the ethyl acetate fraction possessing the highest antioxidant and anti-inflammatory activity. The ethanolic extract and ethyl acetate fraction of &lt;em&gt;A. boonei&lt;/em&gt; stem bark demonstrated significant reductions in the elevated levels of plasma liver function enzymes and were also able to significantly restore the activities of enzyme antioxidants (SOD and catalase). Furthermore, restorations in liver histoarchitecture were observed in groups treated with the extract and fractions. &lt;em&gt;In silico&lt;/em&gt; analysis revealed hi","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"14 ","pages":"Article 100558"},"PeriodicalIF":0.0,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143138022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}