中华医学美学美容杂志最新文献

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Clinical application and effects of two different liposuction methods in breast augmentation by autologous fat grafting 两种吸脂方法在自体脂肪移植术隆胸中的临床应用及效果
Chinese Journal of Medical Aesthetics and Cosmetology Pub Date : 2019-02-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.01.007
Qian Zhang, Jie Shi, Xiaodian Li, Yaguang Tian
{"title":"Clinical application and effects of two different liposuction methods in breast augmentation by autologous fat grafting","authors":"Qian Zhang, Jie Shi, Xiaodian Li, Yaguang Tian","doi":"10.3760/CMA.J.ISSN.1671-0290.2019.01.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2019.01.007","url":null,"abstract":"Objective \u0000To explore the effect of different harvest methods of liposuction on the autologous fat grafting. \u0000 \u0000 \u0000Methods \u0000The clinical data of 60 patients with fat grafting for breast augmentation from January 2014 to January 2017 were analyzed retrospectively. According to the method of liposuction, 60 patients were divided into the water-jet assisted liposuction group (30 cases) and negative-pressure machine liposuction group (30 cases). The surgical time of different liposuctions and the fat survival were compared after breast augmentation. The clinical effect of different liposuction methods was analyzed by follow-up one year after operation. \u0000 \u0000 \u0000Results \u0000Sixty patients completed the surgery. The fat survival rate of water-jet assisted liposuction was (66.71±2.68)%, and the fat survival rate of the negative-pressure machine liposuction was (51.44±1.16)%. There were statistically significant difference between the two groups (P 0.05). \u0000 \u0000 \u0000Conclusions \u0000Compared with negative pressure liposuction, water-jet assisted liposuction using autologous fat grafting in breast augmentation can improve the survival rate of fat transplantation. This technique has good clinical application value. \u0000 \u0000 \u0000Key words: \u0000Transplantation, autologous; Fats; Breast; Negative-pressure liposuction; Water-jet assisted liposuction","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"25 1","pages":"25-28"},"PeriodicalIF":0.0,"publicationDate":"2019-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43471014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of platelet-rich plasma on AnxA1 gene and PPARγ gene of adipose-derived mesenchymal stem cells of rabbit 富血小板血浆对兔脂肪间充质干细胞AnxA1基因和PPARγ基因的影响
Chinese Journal of Medical Aesthetics and Cosmetology Pub Date : 2019-02-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.01.018
Si Wei, G. Hu, Huizhong Guo, Yuquan Wang, Le Kang
{"title":"Effect of platelet-rich plasma on AnxA1 gene and PPARγ gene of adipose-derived mesenchymal stem cells of rabbit","authors":"Si Wei, G. Hu, Huizhong Guo, Yuquan Wang, Le Kang","doi":"10.3760/CMA.J.ISSN.1671-0290.2019.01.018","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2019.01.018","url":null,"abstract":"Objective \u0000To investigate the effect of platelet-rich plasma(PRP) on starting of AnxA1 gene and PPARγ gene of adipose-derived stem cells (ADSCs) in rabbit. \u0000 \u0000 \u0000Methods \u0000Epididymal adipose tissue stem cells from New Zealand white rabbits, and the cells identified by morphology and inducing differentiation, the cells were cultured to the fourth generation, PRP and PPP (platelet-poor plasma) were prepared by traditional centrifugal method from abdominal aortic of rabbit; ADSCs were cultured in culture medium containing PRP (experimental group), PPP (control group) and all medium (blank group) for each 5% for 24 h, 48h and 72 h. Cells of each group were dissociated and total RNA extracted. AnxA1 gene and PPARγ gene were detected by RT-PCR. \u0000 \u0000 \u0000Results \u0000Primary ADSCs of rabbit grew in the way of long spindle swirly. The results of oil red O and alizarin red staining of the ADSCs were positive. AnxA1 gene and PPARγ gene of experimental group significantly increased from the result of RT-PCR (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000PRP can promote proliferation of the ADSCs of rabbit and increase the expression of AnxA1 gene and PPARγ gene significantly. \u0000 \u0000 \u0000Key words: \u0000Platelet-rich plasma; Stem cells; Gene expression; AnxA1 gene; PPARγgene; Adipose-derived mesenchymal stem cells","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"25 1","pages":"64-67"},"PeriodicalIF":0.0,"publicationDate":"2019-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43093753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Directional differentiation of human adipose-derived stem cells into keratin-forming cells 人脂肪干细胞向角蛋白形成细胞的定向分化
Chinese Journal of Medical Aesthetics and Cosmetology Pub Date : 2019-02-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2019.01.004
Wen-bo Yang, Rong Huang, Chen Deng, Zhanjun Lei
{"title":"Directional differentiation of human adipose-derived stem cells into keratin-forming cells","authors":"Wen-bo Yang, Rong Huang, Chen Deng, Zhanjun Lei","doi":"10.3760/CMA.J.ISSN.1671-0290.2019.01.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2019.01.004","url":null,"abstract":"Objective \u0000To optimize the method of the directional differentiation of adipose-derived stem cells into keratinocytes. \u0000 \u0000 \u0000Methods \u0000Adipose-derived stem cells (ADSCs), separated by collagenase digestion method, were isolated and cultured. Then the expression of surface specific markers CD34, CD44 and CD90 were detected by flow cytometer. The effect of different induced mediums cultured for two weeks on the differentiation of ADSCs into KCs was demonstrated: Group 1, the DMEM supplied with 2% FBS and 49% supertant of KCs; group 2, KSFM medium; group 3, DMEM medium supplied with 10% FBS and 5 μM ATRA; 10% FBS DMEM as the control group. Immunofluorescene staining was applied to detect the expression of keratin CK14 and F-actin. \u0000 \u0000 \u0000Results \u0000A flattened fibroblast-like morphology was observed in cells, the positive expression rate of CD34 was 0.08%, while those of CD44 and CD90 were 99%. The cells that could differentiate into osteoblasts and chondrocytes, indicated that the cells were ADSCs. There was no significant change in the cell morphology in the group 1 under the induction medium; about 10% of the cells in group 2 were altered; the morphological changes were obvious in group 3, and approximately 20% of the cells showed irregular polygon. The immunofluorescene staining of the cells in group 3 indicated that the cells showed cobblestone-like phenotype and an organized cytoskeletal network with dense actin fibers at the edges; some cells were positive for CK14. \u0000 \u0000 \u0000Conclusions \u0000ADSCs show higher induction rate under ATRA stimulation. \u0000 \u0000 \u0000Key words: \u0000Adipose tissue; Stem cells; Cell differentiation; Keratinocytes","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"25 1","pages":"13-16"},"PeriodicalIF":0.0,"publicationDate":"2019-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43810316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antagonistic effect of platelet-rich plasma on photo-aged skin in rat 富血小板血浆对大鼠光老化皮肤的拮抗作用
Chinese Journal of Medical Aesthetics and Cosmetology Pub Date : 2018-12-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2018.06.019
Wenbo Wu, Minliang Chen, Yanqiu Wu
{"title":"Antagonistic effect of platelet-rich plasma on photo-aged skin in rat","authors":"Wenbo Wu, Minliang Chen, Yanqiu Wu","doi":"10.3760/CMA.J.ISSN.1671-0290.2018.06.019","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2018.06.019","url":null,"abstract":"Objective \u0000To observe the effect of platelet-rich plasma (PRP) on photoaging skin of rat. \u0000 \u0000 \u0000Methods \u0000F344 rats were used as model animals and skin photoaging model was established by UV irradiation. The model animals were divided into 5 groups: group A was injected with activated PRP (A-PRP); group B was injected with inactive PRP (N-PRP); group C was injected with normal saline; group D was irradiated only with UV; Four weeks after the injection, the appearance and histological characteristics of the rat's skin were evaluated. \u0000 \u0000 \u0000Results \u0000Compared with group C and group D, the wrinkles and skin color in group A and group B were significantly improved. Histological observation showed that the histological features of group A and group B were significantly better than that of group C and D (P 0.05). The thickness of dermis in group E was significantly higher than that in group C (P<0.01). \u0000 \u0000 \u0000Conclusions \u0000Injection of PRP can improve the appearance and histological features of photo-aged skin in rats. A-PRP is better than N-PRP. \u0000 \u0000 \u0000Key words: \u0000Platelet-rich plasma; Light; Cell aging; Skin; Rats; Photoageing","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"24 1","pages":"445-448"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43346652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical effects of en-bloc U-shaped osteotomy of the mandible in prominent mandibular angle patients with long chin 下颌骨整体U形截骨治疗下颌角突出伴长下巴患者的临床疗效
Chinese Journal of Medical Aesthetics and Cosmetology Pub Date : 2018-12-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2018.06.001
Chenzhi Lai, Xiaolei Jin, Xianlei Zong, Guodong Song
{"title":"Clinical effects of en-bloc U-shaped osteotomy of the mandible in prominent mandibular angle patients with long chin","authors":"Chenzhi Lai, Xiaolei Jin, Xianlei Zong, Guodong Song","doi":"10.3760/CMA.J.ISSN.1671-0290.2018.06.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2018.06.001","url":null,"abstract":"Objective \u0000To discuss a surgical treatment to correct prominent mandibular angle with long chin. \u0000 \u0000 \u0000Methods \u0000From July 2015 to October 2017, 21 patients with malocclusion who suffered from prominent mandibular angle with long chin underwent en-bloc mandibular U-shaped osteotomy through oralincision in order to shorten mandibular angle, the body of mandible and the chin and to improve the facial contour. We assessed the surgical effect through comparison of radiologic images, satisfaction of patients and surgical complications between pre-operation and post-operation. \u0000 \u0000 \u0000Results \u0000In this group, all oral incisions had healing by first intention, without hematoma, infection and osteonecrosis. Low lip numbness occurred in 16 patients. After following up for 6-12 months, the lower lip numbness was recovered in 14 patients and relieved in 2 patients. All patients satisfied with the appearance after the operation. \u0000 \u0000 \u0000Conclusions \u0000En-bloc mandibular U-shaped osteotomy is an improvement for mandible surgery treatment. Its osteotomy line involves the whole mandible, which makes the length and width of mandible smaller apparently. This procedure can effectively correct prominent mandibular angle with long chin. \u0000 \u0000 \u0000Key words: \u0000Mandibular osteotomy; Hypertrophy; Maxillofacial abnormalities; Genioplasty; Cosmetic Techniques","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"24 1","pages":"381-385"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41475543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Values of CAD/CAM technology and assistant reconstruction in mandible defect with fibular musculocutaneous flap CAD/CAM技术及腓骨肌皮瓣辅助重建下颌骨缺损的价值
Chinese Journal of Medical Aesthetics and Cosmetology Pub Date : 2018-12-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2018.06.003
Ming Hu, Lei Zhang, Hang-yu Zhou, Linsong Ji, Fuhai Zeng, Yufei Ma
{"title":"Values of CAD/CAM technology and assistant reconstruction in mandible defect with fibular musculocutaneous flap","authors":"Ming Hu, Lei Zhang, Hang-yu Zhou, Linsong Ji, Fuhai Zeng, Yufei Ma","doi":"10.3760/CMA.J.ISSN.1671-0290.2018.06.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2018.06.003","url":null,"abstract":"Objective \u0000To explore the role of computer-aided design and manufacturing technology (CAD/CAM technology) in the repair of mandibular defects using the fibular osteomyocutaneous free flap. \u0000 \u0000 \u0000Methods \u0000Eight patients with a portion of mandible defect due to various reasons were subjected to preoperative CT scan, obtaining CT data of the mandible and the fibular bone to be transplanted. The original and the reconstructed skull model, as well as the mandible and fibula osteotomy guide plates, was printed using CAD/CAM technology. The titanium plates were preformed using the reconstructed skull model, and the operation was performed with the help of guide plates and a preformed titanium plate. The patients were followed up for 6 to 24 months to evaluate the operation effect. \u0000 \u0000 \u0000Results \u0000Two in 8 cases had flap necrosis caused by immune rejection of the implant, and one case had flap necrosis due to poor postoperative blood glucose control. The other 5 patients healed at the primary site and the fibular myocutaneous flap survived well. There were no serious complications in the donor and recepient site. The patients' satisfaction was high. \u0000 \u0000 \u0000Conclusions \u0000The reconstruction of mandible with the aid of CAD/CAM technique has the advantages of accelerating the operation process, shortening the operation time, improving the accuracy of mandibular amputation and fibular shaping, promotlng postoperative function and morphology recovery. \u0000 \u0000 \u0000Key words: \u0000Mandibular reconstruction; Fibula; Myocutaneous flap; Reconstructive surgical procedures; Computer-aided design and manufacturing technology","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"24 1","pages":"391-395"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47989137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical effects of pretarsal fullness reconstruction of the lower eyelid using autologous dermis or acellular dermis 自体真皮与脱细胞真皮重建下眼睑睑前丰满的临床效果
Chinese Journal of Medical Aesthetics and Cosmetology Pub Date : 2018-12-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2018.06.013
Hongbin Xie, Xiang Xie
{"title":"Clinical effects of pretarsal fullness reconstruction of the lower eyelid using autologous dermis or acellular dermis","authors":"Hongbin Xie, Xiang Xie","doi":"10.3760/CMA.J.ISSN.1671-0290.2018.06.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2018.06.013","url":null,"abstract":"Objective \u0000To investigate the new metheds of recreating pretarsal fullness of the lower eyelid using autologous dermis or acellullar dermis. \u0000 \u0000 \u0000Methods \u0000Between September 2010 and November 2013, total 16 patients underwent the procedures of recreating of pretarsal fullness of the lower eyelid using dermis materials. A stab skin incision of approximately 4-5 mm was made at the lateral canthus and inner canthus along the subciliary crease of the lower eyelid respectively, and then the skin was undermined to create a subcutaneous tunnel between two stab incisions. The dermis roll was introduced into the undermined subcutaneous plane of the lower eyelid. The end of dermis was sutured to orbicularis oculi muscle to maintain the appropriate tension. \u0000 \u0000 \u0000Results \u000011 cases of them used autologous dermis, 5 of used acellullar dermis. The patients were followed up for 3-24 months. Lower-eyelid pretarsal fullness regained abvious and natural appearance, and the patients were highly satisfied. Complications included slight sclera show, partial absorption of dermis material and slight irregular apperence. One case of dermis infection occured and dermis roll had to be removed. \u0000 \u0000 \u0000Conclusions \u0000Autologous dermis or acellullar dermis grafting are simple, efficient techniques for recreating of pretarsal fullness of the lower eyelid. \u0000 \u0000 \u0000Key words: \u0000Transplantation, autologous; Dermis; Acellular dermis; Minor surgical procedures; Pretarsal fullness","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"24 1","pages":"426-428"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44019782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevention and treatment of the complications of autograft rib rhinoplasty 自体肋骨鼻整形术后并发症的防治
Chinese Journal of Medical Aesthetics and Cosmetology Pub Date : 2018-12-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2018.06.005
Hengyue Song, Xiancheng Wang, X. Xiong, Yang Sun
{"title":"Prevention and treatment of the complications of autograft rib rhinoplasty","authors":"Hengyue Song, Xiancheng Wang, X. Xiong, Yang Sun","doi":"10.3760/CMA.J.ISSN.1671-0290.2018.06.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2018.06.005","url":null,"abstract":"Objective \u0000To discuss the prevention and treatment of the complications of rib rhinoplasty in Chinese. \u0000 \u0000 \u0000Methods \u0000A total of 209 patients received rib rhinoplasty in which postoperative complications happened in 14 cases from Jan. 2010 to Aug. 2017, and the clinical data were analyzed retrospectively. \u0000 \u0000 \u0000Results \u0000195 patients showed satisfactory results and no complications. Postoperative complications occurred in 14 cases. There were 12 cases of rib cartilage warping (incidence rate was 5.7%). 4 cases were thought that warping was within acceptable range, and 8 cases had satisfactory results after the secondary repair surgery. There were 3 cases of resorption of rib cartilage (incidence rate was 1.4%), of which 2 cases were complicated with cartilage warping and 1 case complicated with infection. None of them underwent repair surgery. The infection complicated with cartilage resorption was 1 case (0.5%). The infection was improved by conservative treatment and the cartilage was not removed. There was 1 case of hypertropic scar on chest wall (incidence rate was 0.5%). \u0000 \u0000 \u0000Conclusions \u0000The warping of cartilage can be significantly reduced by selecting straight section of costal cartilage, preserving the central costal cartilage grafts, symmetrically carving and crosscutting. Meanwhile, sterility and accurate operation can decrease the incidence of infection, aerothorax and scars. \u0000 \u0000 \u0000Key words: \u0000Nose; Surgery, plastic; Costal cartilage; Transplantation, autologous; Postoperative complications","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"24 1","pages":"400-403"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46520614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of lentivirus encoding acidic fibroblast growth factor on cycle and proliferation of adipose-derived stem cells 编码酸性成纤维细胞生长因子的慢病毒对脂肪源性干细胞周期和增殖的影响
Chinese Journal of Medical Aesthetics and Cosmetology Pub Date : 2018-12-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2018.06.020
Yu Wang, A. Zhang, Chang-bo Tao, Caiqi Shen, Yanping Guo, Tao Jiang, Linxia Zhang
{"title":"Effect of lentivirus encoding acidic fibroblast growth factor on cycle and proliferation of adipose-derived stem cells","authors":"Yu Wang, A. Zhang, Chang-bo Tao, Caiqi Shen, Yanping Guo, Tao Jiang, Linxia Zhang","doi":"10.3760/CMA.J.ISSN.1671-0290.2018.06.020","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2018.06.020","url":null,"abstract":"Objective \u0000To study the effect of the lentivirus encoding acidic fibroblast growth factor transfecting human adipose-derived stem cells (ADSCs) on the cell cycle and proliferation of ADSCs. \u0000 \u0000 \u0000Methods \u0000ADSCs were isolated and extracted by enzymatic digestion from the liposuction aspirate. ADSCs were cultured, identified and osteogenic induced reagent was used to induce differentiation of ADSCs towards bone cells. To obtain lentivirus encoding FGF-1, the plasmid PWPXLd-FGF-1 was co-transfected with plasmid psPAX2, pMD2.G in 293T cells. ADSCs were infected with lentivirus encoding FGF-1. Expression of green fluorescent protein (GFP) in infected FGF-1 was observed by fluorescence microscope and expression of FGF-1 in ADSCs was verified by Western blot analysis. Flow cytometry was used to detect the cell cycle of ADSCs infected with lentivirus encoding FGF-1. EDU assay was performed to examine cell viability. \u0000 \u0000 \u0000Results \u0000Lentivirus encoding FGF-1 was obtained. After ADSCs being infected green fluorescence was found in about 70% ADSCs, and overexpression of FGF-1 protein was detected in infceted cells by Western blot analysis. The percentage of G2/M phase cells was significantly increased compared with the control group, and the proliferation of ADSCs infected with lentivirus encoding FGF-1 was promoted as compared with the control group. \u0000 \u0000 \u0000Conclusions \u0000FGF-1 can enhance G2/M phase of ADSCs and promote the proliferation of ADSCs. \u0000 \u0000 \u0000Key words: \u0000Fibroblast growth factor 1; Genes; Lentivirus; Cell proliferation; Cell cycle; Adipose-derived stem cells","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"24 1","pages":"449-453"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44312961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Treatment of mycobacterium tuberculousis induced with cosmetic injection 美容注射治疗结核分枝杆菌感染
Chinese Journal of Medical Aesthetics and Cosmetology Pub Date : 2018-12-15 DOI: 10.3760/CMA.J.ISSN.1671-0290.2018.06.014
Linying Lai, Lixia Zhang, Minliang Chen, H. Duan, Guiwen Zhou
{"title":"Treatment of mycobacterium tuberculousis induced with cosmetic injection","authors":"Linying Lai, Lixia Zhang, Minliang Chen, H. Duan, Guiwen Zhou","doi":"10.3760/CMA.J.ISSN.1671-0290.2018.06.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1671-0290.2018.06.014","url":null,"abstract":"Objective \u0000To explore the diagnosis and treatment of non-tuberculosis mycobacteria (NTM) infection after cosmetic injection via scientific debridement surgery combined with regular application of anti-NTM drugs. \u0000 \u0000 \u0000Methods \u000014 patients who were infected with NTM after cosmetic injection and were not cured over a long period of time in other hospitals from 2012 to 2016. The patients were treated with VSD thorough surgical debridement, the bacterial type of NTM was identified by bacterial culture and PCR identification and anti-NTM drugs were systematically used according to the results of drug sensitivity. \u0000 \u0000 \u0000Results \u0000Fourteen patients who were treated with scientific debridement surgeries combined with regular anti-NTM drug treatment in our hospital for 2-4 months were discharged after their skin lesions were cleared and healed and they were continually treated with anti-NTM drugs for 12 months. Fourteen patients were completely cured by using the above treatments without severe side effects, such as liver and kidney dysfunction, nervous system disorders and so on. Only colpitis mycotica occurred in 3 patients. In addition, one patient presented the decrease of leukopenia after using anti-NTM drugs for 2 months and continued to complete the treatment after we adjusted the treatment plan to returning the level of leukopenia to the normal. These 14 patients were followed up for 1-5 years with no recurrence of the lesion. The facial appearance of 12 patients were almost normal with slight scars. The facial surgery area of 2 patients were uneven and nearly recovered to normal facial appearance by tissue transplantation and photoelectric therapy. \u0000 \u0000 \u0000Conclusions \u0000For the NTM patients caused by invasive procedures such as injection, the comprehensive treatment program, which combined scientific debridement surgery and systematically targeted drug treatment, not only can effectively cure NTM infection, but also minimize secondary injury and restore the patients' appearance, which is worthy of clinical application. \u0000 \u0000 \u0000Key words: \u0000Improving looks; Injections; Mycobacterium infections, nontuberculous; Debridement; Drug therapy","PeriodicalId":10094,"journal":{"name":"Chinese Journal of Medical Aesthetics and Cosmetology","volume":"24 1","pages":"429-431"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49390303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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