Effect of lentivirus encoding acidic fibroblast growth factor on cycle and proliferation of adipose-derived stem cells

Abstract

Objective To study the effect of the lentivirus encoding acidic fibroblast growth factor transfecting human adipose-derived stem cells (ADSCs) on the cell cycle and proliferation of ADSCs. Methods ADSCs were isolated and extracted by enzymatic digestion from the liposuction aspirate. ADSCs were cultured, identified and osteogenic induced reagent was used to induce differentiation of ADSCs towards bone cells. To obtain lentivirus encoding FGF-1, the plasmid PWPXLd-FGF-1 was co-transfected with plasmid psPAX2, pMD2.G in 293T cells. ADSCs were infected with lentivirus encoding FGF-1. Expression of green fluorescent protein (GFP) in infected FGF-1 was observed by fluorescence microscope and expression of FGF-1 in ADSCs was verified by Western blot analysis. Flow cytometry was used to detect the cell cycle of ADSCs infected with lentivirus encoding FGF-1. EDU assay was performed to examine cell viability. Results Lentivirus encoding FGF-1 was obtained. After ADSCs being infected green fluorescence was found in about 70% ADSCs, and overexpression of FGF-1 protein was detected in infceted cells by Western blot analysis. The percentage of G2/M phase cells was significantly increased compared with the control group, and the proliferation of ADSCs infected with lentivirus encoding FGF-1 was promoted as compared with the control group. Conclusions FGF-1 can enhance G2/M phase of ADSCs and promote the proliferation of ADSCs. Key words: Fibroblast growth factor 1; Genes; Lentivirus; Cell proliferation; Cell cycle; Adipose-derived stem cells