Journal of Natural Pesticide Research最新文献

{"title":"Phytotoxic, antifungal and antiplasmodial properties of diterpenes isolated from Copaifera resins","authors":"Victor Pena Ribeiro ,&nbsp;Kumudini Meepagala ,&nbsp;Prabin Tamang ,&nbsp;Jairo Kenupp Bastos ,&nbsp;Joanna Bajsa-Hirschel","doi":"10.1016/j.napere.2025.100161","DOIUrl":"10.1016/j.napere.2025.100161","url":null,"abstract":"<div><div>Diterpenes are bioactive secondary metabolites with diverse pharmacological and agricultural applications. In this study, four diterpenes—<em>ent</em>-polyalthic acid (1), <em>ent</em>-dihydroagathic acid (2), <em>ent</em>-kaurenoic acid (3), and <em>ent</em>-kaurenol (4)—were isolated from copaiba oil-resins and evaluated for their phytotoxic, antifungal, and antiplasmodial activities. Phytotoxicity assays revealed herbicidal activity against <em>Agrostis stolonifera</em>, with <em>ent</em>-polyalthic acid and <em>ent</em>-kaurenoic acid exhibiting the highest efficacy, inhibiting seed germination. <em>ent</em>-dihydroagathic acid also showed phytotoxicity against <em>Lemna paucicostata</em> with IC₅₀ value of 3.8 µM. All compounds inhibited the growth of <em>Colletotrichum fragariae</em> in a bioautography bioassay, with <em>ent</em>-kaurenoic acid exhibiting the highest activity. Molecular docking studies demonstrated favorable binding affinities of <em>ent</em>-polyalthic acid and <em>ent</em>-dihydroagathic acid to <em>Plasmodium falciparum</em> lactate dehydrogenase. Pharmacokinetic assessments indicated adherence to Lipinski’s Rule of Five and suitable properties for oral bioavailability. These findings shows the potential of these diterpenes, particularly <em>ent</em>-polyalthic acid, as lead compound for herbicidal and antimalarial drug development.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"14 ","pages":"Article 100161"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of novel insecticides against fall armyworm (Spodoptera frugiperda) via laboratory bioassay and field experiment findings","authors":"Nabin Bhandari ,&nbsp;Dipesh Kumar Mehata ,&nbsp;Aasish Bhandari ,&nbsp;Ananta Mani Bhattarai","doi":"10.1016/j.napere.2025.100159","DOIUrl":"10.1016/j.napere.2025.100159","url":null,"abstract":"<div><div>Fall armyworm (<em>Spodoptera frugiperda</em>) is a major agricultural pest responsible for significant economic losses globally. Due to the rising concerns over the overuse of synthetic insecticides, there is an increasing demand for safer and more sustainable alternatives. This study evaluated the efficacy of various chemical and biorational insecticides: neem (5 mL L⁻¹), timur extract (5 g L⁻¹), Btk + S. spinosa (2 mL L⁻¹), spinosad (0.3 mL L⁻¹), emamectin benzoate + abamectin (0.4 g L⁻¹), and chlorantraniliprole (0.4 mL L⁻¹) against fall armyworm under both laboratory and field conditions, with water spray serving as a control. Laboratory bioassays were conducted using a leaf dip method in a Completely Randomized Design (CRD) with four replications per treatment and 20 larvae per replication (total = 560 larvae). Leaves were dipped in each insecticide solution at recommended concentrations. Complete mortality within 12 h was observed for Btk + S. spinosa, spinosad, and Ema. + Aba., while chlorantraniliprole and neem required 42 and 84 h, respectively. Field trials followed a Randomized Complete Block Design (RCBD) with seven treatments and three replications. Insecticides were applied at specific label-recommended rates at 25, 35, and 45 days after sowing (DAS) when infestation exceeded the economic threshold level. Ema. + Aba., chlorantraniliprole, and spinosad significantly reduced larval population and foliar damage, while enhancing maize growth and yield. These findings suggest that selected insecticides, particularly spinosad, Ema. + Aba., and chlorantraniliprole, are effective and economically viable options for integrated fall armyworm management in maize.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"14 ","pages":"Article 100159"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the power of microbes for plant protection in regenerative agriculture: A comprehensive review","authors":"A. Sowmiya ,&nbsp;M. Suganthy ,&nbsp;R. Saranya ,&nbsp;K. Ganesan ,&nbsp;P. Janaki","doi":"10.1016/j.napere.2025.100170","DOIUrl":"10.1016/j.napere.2025.100170","url":null,"abstract":"<div><div>The shift toward regenerative agriculture has intensified interest in sustainable plant protection strategies that prioritize ecological balance, soil health and long-term productivity. Among these, the use of beneficial microbes has garnered significant attention for their ability to enhance plant defense, suppress pests and diseases and improve overall crop resilience. This review highlights the growing relevance of microbial agents in advancing sustainable plant protection and examines their potential applications within regenerative agricultural systems that emphasize reduced chemical inputs and ecological harmony. It explores key microbial groups including plant growth-promoting rhizobacteria (PGPR), mycorrhizal fungi, endophytes and biocontrol agents and extrapolates their contributions to plant health through mechanisms such as nitrogen fixation, phosphorus solubilization, phytohormone production, systemic resistance induction and antagonism of pathogens and insect pests. Particular emphasis is placed on entomopathogenic fungi, <em>Beauveria bassiana</em>, <em>Metarhizium spp</em>., <em>Isaria spp</em>., and <em>Verticillium spp</em>., the bacteria, <em>Bacillus thuringiensis</em>, entomopathogenic nematodes, <em>Heterorhabditis</em> and <em>Steinernema spp</em>. and entomopathogenic protozoa, <em>Nosema spp</em>., all of which have demonstrated efficacy in controlling a broad spectrum of insect pests. These microbes act through multiple mechanisms, including enzymatic degradation of the insect cuticle, toxin-mediated mortality, interference with host metabolism, septicemia induced by nematode–bacterial symbiosis and long-term host suppression by protozoan infection, making them versatile agents in integrated pest management strategies. This review critically examines their efficacy, specificity, advantages and limitations under both laboratory and field conditions. It further explores challenges related to formulation stability, environmental persistence, delivery systems and compatibility with regenerative practices such as organic amendments and conservation tillage. Real-world case studies and field-based evidence are presented to underscore the practical relevance and scalability of microbial-based pest management. The review concludes that beneficial microbes not only offer effective pest suppression but also enhance plant growth and stress resilience, positioning them as vital tools for regenerative agriculture. Their integration into farming systems represents a biologically sound, ecologically responsible and scalable pathway toward sustainable agricultural transformation.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"14 ","pages":"Article 100170"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145424571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of pestalotioid fungus Truncatella angustata isolated from the infected leaf of rubber plant with leaves fall disease, and its inhibition by Trichoderma spp. in vitro","authors":"Tristan B. Alexander ,&nbsp;Margaretta Christita ,&nbsp;Rumella Simarmata ,&nbsp;Yeni Khairina ,&nbsp;Fiqriah Hanum Khumairah ,&nbsp;Muhammad Ilyas ,&nbsp;Sylvia J.R. Lekatompessy ,&nbsp;Karmila Parakkasi ,&nbsp;Hasanuddin Hasanuddin ,&nbsp;Mukrimin Mukrimin","doi":"10.1016/j.napere.2025.100150","DOIUrl":"10.1016/j.napere.2025.100150","url":null,"abstract":"<div><div>Rubber, a global industrial commodity, continues to increase in demand on the world market. However, the rubber industry faces significant challenges due to pathogen attacks, particularly in the roots and leaves of the rubber tree, leading to reduced productivity and affecting the supply of raw rubber materials. Several types of pathogenic fungi have been studied as causes of disease in rubber plants. This research presents the first report of pestalotioid fungus <em>Truncatella angustata</em> causing leaf fall disease on rubber plants in Indonesia and the inhibition of the fungus by <em>Trichoderma</em> spp. in vitro. In this study, <em>T. angustata</em> was isolated from symptomatic leaves suspected of leaf fall disease on rubber plantations in Jambi Province, Indonesia. This research also investigated the characteristics and mechanisms of inhibition of <em>T. angustata</em> by <em>Trichoderma asperellum</em> isolated from soil at the exact rubber plantation location. As a comparison, <em>Trichoderma koningiopsis</em> from our laboratory collection was used. The investigation results show that the inhibition mechanism is through entanglement. Hence, <em>T. asperellum</em> showed an inhibitory ability of 76.61 %, while <em>T. koningiopsis</em> showed 59.65 %.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"14 ","pages":"Article 100150"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathology, ultrastructure, and biochemical deformities in clover grasshopper Eyprepocnemis plorans plorans (Orthoptera, Acrididae) eggs after exposure to their parents' frass solution","authors":"Zeinab Fathy ,&nbsp;Ayat I. El-Kholy ,&nbsp;Salem M.M. Hagar ,&nbsp;Jihan Muhammad","doi":"10.1016/j.napere.2025.100151","DOIUrl":"10.1016/j.napere.2025.100151","url":null,"abstract":"&lt;div&gt;&lt;div&gt;The clover grasshopper, &lt;em&gt;Eyprepocnemis plorans plorans&lt;/em&gt; (Orthoptera: Acrididae), has emerged as a persistent pest of maize and other crops in Dakahlia Governorate, Egypt, necessitating novel and sustainable control methods. Previous studies have shown that grasshopper fecal extracts can disrupt oviposition and impair egg development in various Orthopteran species. As reported by Tanaka et al. (2019), frass extracts from desert locusts significantly reduced hatching rates and caused embryonic malformations. Similarly, Sugahara et al. (2021) demonstrated that fecal chemicals from &lt;em&gt;Locusta migratoria&lt;/em&gt; altered nutrient distribution within eggs, leading to developmental failure. These findings provide a strong basis for evaluating the biological impact of frass solutions on &lt;em&gt;E. plorans plorans&lt;/em&gt; eggs in our study. Therefore, we aimed to explore the impact of the frass solution on the development of clover grasshopper eggs. The frass solution was prepared by soaking 30 g of dried frass in 300 ml of water overnight and filtering it. The extract was then mixed with sterilized sand containing grasshopper egg pods. The study investigated the inhibitory effects of a conspecific frass solution derived from the dried fecal matter of &lt;em&gt;E. plorans plorans&lt;/em&gt; on the development and viability of the species’ eggs under greenhouse of the Plant Protection Research Institute, Agricultural Research Center in Giza, Egypt. Conditions were maintained at 30–35 °C and ∼70 % relative humidity for 28 days, which corresponds to the average hatching period for grasshopper eggs.&lt;/div&gt;&lt;div&gt;Microscopic, histopathological, and ultrastructural (transmission electron microscopy) analysis revealed profound structural disruptions in frass-treated eggs: the chorion (eggshell) exhibited thinning, fragmentation, and increased permeability, the vitelline membrane was disrupted, and marked degeneration of the micropylar apparatus, a structure essential for sperm entry and gas exchange, was observed. Embryos within treated eggs were amorphous or undeveloped, displaying sparse, abnormally distributed yolk and deteriorated cytoplasmic structures.&lt;/div&gt;&lt;div&gt;The frass solution treatment significantly reduced total protein (df = 1, F = 775.84, P &lt; 0.001), carbohydrate (df = 1, F = 768, P &lt; 0.001), and lipid contents (df = 1, F = 144.5, P &lt; 0.001) in clover grasshopper eggs compared to controls. Over the four weeks, significant temporal changes were observed within both control and treated groups for protein (control: df = 3, F = 417.63, P &lt; 0.001; treatment: df = 3, F = 722, P &lt; 0.001), carbohydrate (control: df = 3, F = 1.86, P &lt; 0.001; treatment: df = 1, F = 99.66, P &lt; 0.001), and lipid contents (control: df = 3, F = 188, P &lt; 0.001; treatment: df = 3, F = 44.75, P &lt; 0.001), indicating that the biochemical composition changed significantly over time in both groups. In conclusion, this study contributes important knowledge on the use ","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"14 ","pages":"Article 100151"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of rice leaf folder (Cnaphalocrocis medinalis Guenée) in Bangladesh: Evaluating the pesticidal efficacy of some botanicals an eco-friendly alternative","authors":"Tapon Kumar Roy ,&nbsp;Md. Asib Biswas ,&nbsp;S.M.M. Shahriar Tonmoy ,&nbsp;Abu Nayeem ,&nbsp;Md Mosaddek Hossain ,&nbsp;Anamika Sannal","doi":"10.1016/j.napere.2025.100152","DOIUrl":"10.1016/j.napere.2025.100152","url":null,"abstract":"<div><div>The rice leaf folder (RLF) is one of the major insect pests of rice, causing significant yield losses and management of this pests mostly depends on synthetic insecticide applications, which poses environmental and health hazards. This study aimed to evaluate the efficacy of selected botanical insecticides compared to a synthetic insecticide for managing RLF populations under both laboratory and in planta conditions. The study employed a Complete Randomized Design with three replications and eight treatments: evaluating six botanicals including neem oil (2.50 % concentration) and mahogany seed kernel powder (10 % concentration), comparing with one synthetic insecticide Curtap and one untreated control. Results indicated that the synthetic insecticide ‘Curtap’ achieved the highest larval mortality (93.33 %) under both conditions, followed closely by neem oil (90.00 % in laboratory and 83.33 % in planta), indicating its potential as an effective botanical insecticide at 72 h after treatment. Mahogany seed kernel powder also exhibited significant efficacy, causing 70.00 % and 66.67 % mortality in laboratory and in planta conditions, respectively. While other botanicals demonstrated comparatively lower efficacy. Both neem oil and Curtap significantly reduced larval weight (0.63 mg and 0.38 mg) and adult longevity (4.33 days and 3.67 days), while untreated controls exhibited the longest longevity (11.33 days), indicating their inhibitory impact on RLF growth, development, feeding behavior and reproductive suppression. The F₁ adult emergence was completely inhibited by Curtap, while neem oil and mahogany seed kernel powder significantly reduced F₁ emergence to 1.33 adults, exhibiting their potential to disrupt RLF reproduction. Present findings highlight the potential of neem oil followed by mahogany seed kernel powder, as eco-friendly alternatives to synthetic insecticides for RLF management. Integrating these botanicals into insect pest management strategies, specifically for RLF, could reduce pesticide dependency while ensuring sustainable rice production.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"14 ","pages":"Article 100152"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144987922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbivore-induced pulse crop volatiles: Roles in non-nutritiveness to Spodoptera litura fitness and parasitoid attraction","authors":"Ramasamy Kanagaraj Murali-Baskaran,&nbsp;Yogesh Yele,&nbsp;Kailash Chander Sharma","doi":"10.1016/j.napere.2025.100155","DOIUrl":"10.1016/j.napere.2025.100155","url":null,"abstract":"<div><div>Herbivore-induced plant volatiles (HIPVs) emitted by pulse crops in response to <em>Spodoptera litura</em> infestation remain poorly understood in terms of their ecological functions. In this study, volatile profiles of S. <em>litura</em>-infested and -uninfested seedlings of cowpea, green gram, black gram, and soybean were analyzed using a dynamic plant volatile trapping system coupled with GC–MS. The dual roles of major volatiles were further examined by assessing their effects on <em>S. litura</em> feeding performance and the foraging behavior of its egg parasitoid <em>Trichogramma japonicum</em>. Seven synthetic volatiles were incorporated into artificial diets at 500 ppm and tested against second-instar of <em>S. litura</em> for growth indices. Diets containing octadecane and eicosane significantly reduced larval weight gain, diet consumption, and frass production. Octadecane-treated diets caused reductions of 49 % in relative growth rate (RGR), 74 % in relative consumption rate (RCR), and 52 % in approximate digestibility (AD), while eicosane-treated diets reduced RGR by 31 %, RCR by 62 % and AD by 37 %. Additionally, both compounds enhanced the foraging activity of <em>T. japonicum</em> on <em>S. litura</em> eggs. These findings demonstrate the ecological significance of HIPVs, with octadecane and eicosane functioning dually as feeding deterrents for <em>S. litura</em> and attractants for natural enemy. The results provide valuable insights into HIPV-mediated interactions and highlight their potential application in sustainable insect pest management.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"14 ","pages":"Article 100155"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145104301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Latex proteins from Calotropis procera elicit biochemical defenses in banana plantlets against Fusarium kalimantanense","authors":"Isabel Cristina Costa Souza ,&nbsp;Christiana de Fátima Bruce da Silva ,&nbsp;Celli Rodrigues Muniz ,&nbsp;Cristina Paiva da Silveira Carvalho ,&nbsp;Cleverson Diniz Teixeira de Freitas ,&nbsp;José Francisco de Carvalho Gonçalves ,&nbsp;Thalles Barbosa Grangeiro ,&nbsp;Cléberson de Freitas Fernandes ,&nbsp;Márcio Viana Ramos","doi":"10.1016/j.napere.2025.100157","DOIUrl":"10.1016/j.napere.2025.100157","url":null,"abstract":"<div><div>Panama disease induces physiological and morphological symptoms, as well as tissue damage, in banana varieties. The infection impairs plant growth and yield, and in advanced stages, it leads to mortality. No definitive protocol is established for the management of its infectious agent. In this investigation, healthy banana plantlets were treated with either water, 1.5 mM methyl jasmonate (MeJa), or <em>Calotropis procera</em> latex proteins (CpLp, 0.15 mg.ml⁻¹). CpLp consists of soluble proteins extracted from <em>C. procera</em> latex that exhibit established synergistic effects against phytopathogens. After seven days, the pathogen <em>Fusarium kalimantanense</em> (10⁶ conidia.ml⁻¹) was inoculated. Symptoms of illness were evaluated through scanning electron microscopy and lipid peroxidation, and antioxidant enzyme activities were assessed 28 days after inoculation. Plants that received only water showed severe disease symptoms, whereas only 20 % of those treated with CpLp or MeJa exhibited signs of disease. The disease severity index in these groups was minimal, and the growth rate was similar to that of the non-inoculated plants. Ascorbate peroxidase activity increased solely in healthy plants exposed to MeJa or CpLp, while catalase and guaiacol peroxidase activities were enhanced exclusively in infected plantlets treated with CpLp and MeJa. The concentration of malondialdehyde increased in the infected plants, but it remained stable in the infected plantlets that were treated with CpLp or MeJa. The results indicated that exogenous latex proteins (CpLp), when applied to leaves, triggered a protective response against <em>F. kalimantanense</em> damage in banana plantlets, preserving oxidative balance and promoting plant survival and growth. Thus, in addition to its direct impact on phytopathogens, exogenous latex proteins seem to stimulate defensive systems that alleviate the symptoms of Panama disease.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"14 ","pages":"Article 100157"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145104300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical and molecular profile alterations in Locusta migratoria migratorioides (Orthoptera: Acrididae) induced by the entomopathogenic fungi","authors":"Jihan Muhammad","doi":"10.1016/j.napere.2025.100154","DOIUrl":"10.1016/j.napere.2025.100154","url":null,"abstract":"<div><div><em>Locusta migratoria</em> (Linnaeus, 1758) (Orthoptera: Acrididae) poses a significant agroecological threat due to its density-dependent phase polymorphism, exhibiting distinct phenotypic variations (e.g., in morphology, physiology, and behavior) in response to changes in population density, culminating in migratory swarms that inflict substantial agricultural losses across Afro-Eurasia. This study evaluated the impact of two entomopathogenic fungi, Beauveria bassiana (AUMC9899) and Metarhizium anisopliae (AUCM5130), on the biochemical and molecular profiles of L. migratoria under semi-field conditions. Both fungi demonstrated considerable insecticidal efficacy. B. bassiana induced nymphal mortality rates of 48.30 % at its LC₅₀ and 87.33 % at its LC₉₀ after seven days, while M. anisopliae similarly resulted in 47 % and 85 % nymphal mortality at these respective concentrations. At their respective LC₅₀ concentrations (1.7 × 10⁵ and 1.7 × 10⁶ conidia/mL), both B. bassiana and M. anisopliae caused significant reductions in essential nutrient reserves by day 7. Total protein was reduced by 51.10 % (B. bassiana) and 53.39 % (M. anisopliae). Total carbohydrates decreased by 12.98 % (B. bassiana) and 18.26 % (M. anisopliae), while total lipids were reduced by 24.58 % (B. bassiana) and 38.14 % (M. anisopliae). These changes were accompanied by altered key enzyme activities in whole-body homogenates, including increased protease and chitinase and decreased trehalase. Furthermore, SDS-PAGE analysis of hemolymph revealed shared and distinct changes in protein profiles, indicating complex host-pathogen interactions. Both treatments led to the disappearance of higher molecular weight bands (∼232 kDa). B. bassiana developed a distinct band at ∼210 kDa on day 5, which was not observed in the control. While M. anisopliae exhibited a band at ∼212 kDa at the same day 5 time point. Furthermore, M. anisopliae exhibited bands in the ∼47–65 kDa range on days 3 and 5 that were no longer detectable by day 7, similar to B. bassiana, which also showed a reduction in this range by day 7, demonstrating pathogen-specific responses. These findings provide a deeper understanding of the insecticidal mechanisms of these entomopathogenic fungi and highlight their potential for biological control strategies against L. migratoria.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"14 ","pages":"Article 100154"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145104303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autochthonous isolate Pseudomonas 42P4 as a sustainable strategy to control Rhizoctonia solani and promote growth of tomato plants","authors":"María Micaela Pérez-Rodriguez ,&nbsp;Gabriela Lucero ,&nbsp;Betina Agaras ,&nbsp;Miguel Andrés Lobato-Ureche ,&nbsp;María de los Ángeles Fernández ,&nbsp;Magdalena Espino ,&nbsp;Ana Carmen Cohen","doi":"10.1016/j.napere.2025.100164","DOIUrl":"10.1016/j.napere.2025.100164","url":null,"abstract":"<div><div>The fungus <em>Rhizoctonia solani</em> is capable of attacking tomato crops leading to great economic losses. Recently, there is the requirement for agricultural strategies to produce and protect crops with a sustainable approach. The integration of Plant Growth-Promoting Rhizobacteria (PGPR) into agricultural practices has emerged as a biocontrol approach. The aim of this work was to evaluate the <em>in vitro</em> antagonistic activity of native PGPR strains from Mendoza (Argentina) against pathogens of tomato seedlings, and the evaluation of the effect of the most effective PGPR by inoculating tomato seedlings infected with <em>R. solani</em> under greenhouse conditions. <em>Pseudomonas</em> 42P4 isolate (<em>Ps</em> 42P4) belongs to the <em>P. corrugata</em> subgroup of the <em>P. fluorescens</em> complex. <em>Ps</em> 42P4 exhibited more biocontrol traits than the rest of the isolates, was able to grow in presence of fungicides and was the most effective strain in reducing <em>in vitro</em> the growth of all pathogens evaluated. The application of <em>Ps</em> 42P4 reduced the disease incidence and severity with respect to the pathogen infected plants and in a similar way to the chemical treatment and the combination of both. <em>Ps</em> 42P4 increased tomato seedling growth under both healthy as well as diseased conditions. The strain keeps the ability to increase overall tomato growth parameters even in combination with the chemical fungicide, under biotic and non-biotic stress. Our evidence suggests that plant protection is due mainly to increasing the antioxidant enzyme activities, and by modulating the profile of phenolic compounds. In conclusion, <em>Ps</em> 42P4 is a potential strain to be considered as a biocontrol agent against <em>R. solani</em> in tomato plants.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"14 ","pages":"Article 100164"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}