hLife Pub Date : 2025-10-01 Epub Date: 2025-05-24 DOI: 10.1016/j.hlife.2025.05.008

Zhaokai Zhou , Yingying Lv , Anning Zuo , Xudong Zhu , Yudi Xu , Lulu Zuo , Hui Xu , Shutong Liu , Yuyuan Zhang , Siyuan Weng , Yuhao Ba , Peng Luo , Quan Cheng , Jiaxin Xu , Xing Zhou , Shuang Chen , Boxuan Li , Chuhan Zhang , Yukang Chen , Jinhai Deng , Xinwei Han

{"title":"Interactions between microbiota and innate immunity in tumor microenvironment: Novel insights into cancer progression and immunotherapy","authors":"Zhaokai Zhou , Yingying Lv , Anning Zuo , Xudong Zhu , Yudi Xu , Lulu Zuo , Hui Xu , Shutong Liu , Yuyuan Zhang , Siyuan Weng , Yuhao Ba , Peng Luo , Quan Cheng , Jiaxin Xu , Xing Zhou , Shuang Chen , Boxuan Li , Chuhan Zhang , Yukang Chen , Jinhai Deng , Xinwei Han","doi":"10.1016/j.hlife.2025.05.008","DOIUrl":"10.1016/j.hlife.2025.05.008","url":null,"abstract":"<div><div>Human microbiota constitute a complex and dynamic community that interacts with the innate immunity of the host at various anatomical sites, influencing both physiological and pathological states. In individuals with a genetic predisposition, disruptions to the “innate immunity‒microbiota” axis appear to rewire immune responses within the tumor microenvironment (TME), thereby driving cancer pathogenesis. This review summarizes the intricate crosstalk between the microbiota and innate immunity in both healthy and cancerous states, focusing on the modulation of immune recognition and polarization during tumor progression, including immune escape, barrier disruption, chronic inflammatory transformation, and angiogenesis in the initiation phase, as well as the regulation of local invasion, vascular invasion, and pre-metastatic ecological niche formation involved in the metastatic phase. This review also highlights recent advances and challenges in leveraging microbiota for cancer immunotherapy, covering innovations in bacteriophages, genetically engineered probiotics, and bioinformatic applications. Moreover, this review proposes potential approaches to enhance therapeutic efficacy by targeting innate immunity‒microbiota interactions. Further mechanistic insights into these interactions may pave the way for developing innovative microbiota-based cancer immunotherapies.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 10","pages":"Pages 462-493"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145271430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The promise of synthetic bacteria in cancer immunotherapy: Revitalizing tumor immunity via IL-10R modulation 合成细菌在癌症免疫治疗中的应用前景：通过IL-10R调节激活肿瘤免疫

hLife Pub Date : 2025-10-01 Epub Date: 2025-06-04 DOI: 10.1016/j.hlife.2025.05.013

Jiahe Li , Allen P. Liu

引用次数: 0

Carbapenem-resistant Klebsiella pneumoniae ST11 index from a single strain enhances rapid parallel evolution during persistent infection 单一菌株耐碳青霉烯肺炎克雷伯菌ST11指数增强了持续感染期间的快速平行进化

hLife Pub Date : 2025-10-01 Epub Date: 2025-04-25 DOI: 10.1016/j.hlife.2025.04.007

Liqiang Li , Zhaofang Jiang , Xingwei Wang , Si Yang, Fei Li, Yanyu Xiao, Jiuxin Qu

引用次数: 0

Unveiling the multifaceted roles of ISG15 and ISGylation in copper metabolism and cuproptosis 揭示ISG15和isg酰化在铜代谢和铜沉积中的多方面作用

hLife Pub Date : 2025-10-01 Epub Date: 2025-05-15 DOI: 10.1016/j.hlife.2025.05.003

Junqi Xu , Qiao Zhang , Ismail Mohamed Suleiman , Jianping Xie

引用次数: 0

Structural insights from the bacterial world: Coordination of transcription–translation coupling and transcription termination in archaea 来自细菌世界的结构见解：古生菌中转录-翻译耦合和转录终止的协调

hLife Pub Date : 2025-10-01 Epub Date: 2025-05-24 DOI: 10.1016/j.hlife.2025.05.007

Ruoxuan Li , Vadim Molodtsov , Chengyuan Wang

引用次数: 0

Convergence of carbapenem resistance and hypervirulence in Klebsiella pneumoniae: An emerging public health threat 肺炎克雷伯菌碳青霉烯耐药和高毒力的趋同：一个新出现的公共卫生威胁

hLife Pub Date : 2025-10-01 Epub Date: 2025-05-22 DOI: 10.1016/j.hlife.2025.05.006

Jianping Jiang , Jianfeng Zhang , Zhewei Sun , Liang Chen , Minggui Wang

引用次数: 0

RmpA drives metabolic reprogramming to modulate the phenotypic switch between hypermucoviscosity and biofilm formation in hypervirulent Klebsiella pneumoniae RmpA驱动代谢重编程，以调节高致病性肺炎克雷伯菌高黏性和生物膜形成之间的表型转换

hLife Pub Date : 2025-10-01 Epub Date: 2025-06-19 DOI: 10.1016/j.hlife.2025.06.005

Shigang Yao , Jiangqing Huang , Jianing Geng , Dawei Wei , Chao Wang , Yuqin Song , Gang Zhang , Jie Feng

{"title":"RmpA drives metabolic reprogramming to modulate the phenotypic switch between hypermucoviscosity and biofilm formation in hypervirulent Klebsiella pneumoniae","authors":"Shigang Yao , Jiangqing Huang , Jianing Geng , Dawei Wei , Chao Wang , Yuqin Song , Gang Zhang , Jie Feng","doi":"10.1016/j.hlife.2025.06.005","DOIUrl":"10.1016/j.hlife.2025.06.005","url":null,"abstract":"<div><div>Hypervirulent <em>Klebsiella pneumoniae</em> (hvKp) is a major cause of severe community-acquired infection. A key plasmid-encoded factor, regulator of mucoid phenotype A (RmpA), activates capsule locus gene expression to promote hypermucoviscosity, a phenotype that is strongly linked to increased pathogenicity. However, the precise regulatory mechanisms that control RmpA in hvKp remain poorly understood. In this study, we constructed a <em>rmpA</em> knockdown strain in hvKp using CRISPR interference and assessed the global regulatory role of RmpA through complementary multi-omic sequencing (RNA sequencing [RNA-seq] and chromatin immunoprecipitation sequencing [ChIP-seq]), promoter-<em>gfp</em> reporter assays, and phenotypic experiments. The functional role of RmpA was evaluated in <em>Escherichia coli</em> <em>via</em> heterologous expression. The RNA-seq analysis revealed that RmpA activates carbohydrate metabolism pathways, but represses pathways related to DNA replication, ribosome metabolism, and biofilm formation. The ChIP-seq analysis further confirmed the potential role of RmpA as a global regulator that enhances capsule production by activating transcripts within the capsule locus. It also upregulates genes involved in sugar metabolism and transport, which supplies essential precursors for capsule synthesis. RmpA modulates the phenotypic switch between hypermucoviscosity and biofilm formation by repressing type III fimbriae genes. Notably, RmpA overexpression in <em>E</em>. <em>coli</em> induced changes in multiple metabolic pathways. These findings position RmpA as a latent central regulator in hvKp that orchestrates the metabolic pathways and phenotypic traits essential for virulence.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 10","pages":"Pages 504-516"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145271431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection and engineering of broad-spectrum antiviral affibody peptides against SARS-CoV-2 variants 针对SARS-CoV-2变体的广谱抗病毒附体肽的选择和工程设计

hLife Pub Date : 2025-09-01 Epub Date: 2025-04-30 DOI: 10.1016/j.hlife.2025.04.010

Jinlei Yang , Min Wang , Zhao Chen , Yongqi Li , Qi Peng , Jinlong Cheng , Xin Zhao , Jincun Zhao , Christopher John Hipolito , Jianxun Qi , Yi Shi

引用次数: 0

Palmitic acid esters of hydroxy stearic acids suppress SARS-CoV-2 infection through inhibiting the non-canonical inflammasome 羟基硬脂酸棕榈酸酯通过抑制非典型炎性体抑制SARS-CoV-2感染

hLife Pub Date : 2025-09-01 Epub Date: 2025-02-18 DOI: 10.1016/j.hlife.2025.01.005

Chun Kong , Yi Li , Yan Qian , Pengyan Xia

引用次数: 0

Systemic profiling of immune responses in healthy adults vaccinated with an RBD-targeting COVID-19 mRNA vaccine 接种rbd靶向COVID-19 mRNA疫苗的健康成人免疫反应的系统分析

hLife Pub Date : 2025-09-01 Epub Date: 2025-04-26 DOI: 10.1016/j.hlife.2025.04.008

Chao Zhou , Mengxu Sun , Xingyao Huang , Pu Liu , Zhangyi Ouyang , Mei Wu , Lin Yuan , Jingjing Chen , Wenjie Shu , Tianshu Cao , Xiaoyan Wu , Hui Zhao , Cheng-Feng Qin

{"title":"Systemic profiling of immune responses in healthy adults vaccinated with an RBD-targeting COVID-19 mRNA vaccine","authors":"Chao Zhou , Mengxu Sun , Xingyao Huang , Pu Liu , Zhangyi Ouyang , Mei Wu , Lin Yuan , Jingjing Chen , Wenjie Shu , Tianshu Cao , Xiaoyan Wu , Hui Zhao , Cheng-Feng Qin","doi":"10.1016/j.hlife.2025.04.008","DOIUrl":"10.1016/j.hlife.2025.04.008","url":null,"abstract":"<div><div>Coronavirus disease 2019 (COVID-19) messenger RNA (mRNA) vaccines have succeeded unprecedentedly due to high protection efficacy and robust immune responses. However, systematic and longitudinal profiling of immune responses in mRNA vaccine recipients remains limited. Here, a cohort of ten healthy volunteers who received two doses of ARCoV, a non-modified mRNA vaccine, were enrolled. Peripheral blood samples were collected and analyzed using Olink technology, antibody detection, intracellular cytokine staining, single-cell sequencing, and T cell receptor (TCR) sequencing. ARCoV vaccination induced potent humoral and cellular immune responses, as well as elevated cytokines including C-X-C motif chemokine ligand 10 (CXCL10) and interferon-gamma (IFN-γ). Single-cell sequencing revealed that ARCoV immunization induced an increased relative abundance of interferon-activated T cells, proliferative T cells, and naïve T cells. Monocytes and dendritic cells exhibited activation of the innate immune response, downregulation of hypoxia and glycolysis pathways, and a transient decrease in their proportions. Integrative analysis of single-cell RNA and TCR sequencing identified clonal expansion of effector T cells and killer cell immunoglobulin-like receptor (KIR)-expressing natural killer-like cells after the second dose. These findings deepen our understanding of the immune dynamics following mRNA vaccination and offer valuable insights for designing next-generation vaccines. This study was registered at the Chinese Clinical Trial Registry (ChiCTR2100049104).</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 9","pages":"Pages 415-432"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

hLife最新文献