hLife最新文献_第10页

Mucosal vaccine development for respiratory viral infections 针对呼吸道病毒感染的粘膜疫苗开发

hLife Pub Date : 2024-02-01 DOI: 10.1016/j.hlife.2023.12.005

Yifan Lin , Zhenxiang Hu , Yang-Xin Fu , Hua Peng

{"title":"Mucosal vaccine development for respiratory viral infections","authors":"Yifan Lin , Zhenxiang Hu , Yang-Xin Fu , Hua Peng","doi":"10.1016/j.hlife.2023.12.005","DOIUrl":"10.1016/j.hlife.2023.12.005","url":null,"abstract":"<div><p>Mucosal vaccines have risen to prominence in the corona virus disease 2019 (COVID-19) pandemic due to their ability to elicit both local antibody and tissue-resident T cell responses, affording a dual-layered defense against infection and transmission at respiratory entry sites. While intramuscular vaccines predominantly focus on systemic immunity, mucosal vaccines offer a more nuanced, site-specific approach. However, the field faces a dearth of mucosal vaccine options for respiratory diseases, starkly contrasting to the extensive array of well-characterized injectable vaccines. The unique features of mucosal surfaces necessitate specialized adjuvants and delivery systems, adding complexity to adapting injectable vaccine technologies for mucosal applications. Here, we review the recent insights into the specificities of respiratory mucosal immunology that provide a foundation for future innovations besides the emerging vaccine platforms, newly discovered adjuvants, and vaccine delivery systems, which may open promising avenues for developing mucosal vaccines targeting respiratory pathogens.</p></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 2","pages":"Pages 50-63"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949928323000378/pdfft?md5=8ffe7a7a18e2adb9d50bcdfca868c959&pid=1-s2.0-S2949928323000378-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139023278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative interactomic of CD40 in primary B cells 原代 B 细胞中 CD40 的定量交互组学

hLife Pub Date : 2024-02-01 DOI: 10.1016/j.hlife.2023.10.009

Jeremy Argenty , Emilie Maturin , Mylene Camus , Valentin Mellado , Jeanne Perroteau , Benoit Pasquier , Guillaume Voisinne , Odile Burlet-Schiltz , Lih-Ling Lin , Anne Gonzalez de Peredo , Romain Roncagalli , Bernard Malissen

引用次数: 0

Getting ready for the next inforuses 为下一次注入做好准备

hLife Pub Date : 2024-02-01 DOI: 10.1016/j.hlife.2024.01.001

André Costa Lobato

引用次数: 0

Lycorine derivative effectively inhibits the replication of coronaviruses both in vitro and in vivo 番茄红素衍生物能有效抑制冠状病毒在体外和体内的复制

hLife Pub Date : 2024-02-01 DOI: 10.1016/j.hlife.2023.12.001

Liang Shen , Jianzhong Zhao , Ying Xia , Junjie Lu , Jiali Sun , Jian Tang , Hui Xing , Lijuan Yin , Yang Yang , Chunhua Wang

{"title":"Lycorine derivative effectively inhibits the replication of coronaviruses both in vitro and in vivo","authors":"Liang Shen , Jianzhong Zhao , Ying Xia , Junjie Lu , Jiali Sun , Jian Tang , Hui Xing , Lijuan Yin , Yang Yang , Chunhua Wang","doi":"10.1016/j.hlife.2023.12.001","DOIUrl":"10.1016/j.hlife.2023.12.001","url":null,"abstract":"<div><p>The established and ongoing prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and seasonal human coronaviruses (HCoV) like HCoV-OC43, HCoV-NL63, and HCoV-229E, pose a continuous threat to public health. Therefore, it is urgently needed to explore antiviral drugs with broad-spectrum anti-coronavirus activity. Our previous studies have revealed that lycorine is a potent broad-spectrum anti-coronavirus drug, a natural alkaloid extracted from <em>Amaryllidaceae</em> with various pharmacological and microbiological effects. However, it is unsafe to directly use lycorine as a clinical antiviral drug due to the cytotoxicity and induction of cell apoptosis. In this study, a series of lycorine derivatives were designed and synthesized. One of them, named Ly-8, was found to effectively inhibit the replication of different coronavirus strains <em>in vitro</em>, including SARS-CoV-2. Moreover, Ly-8 was also shown to effectively inhibit HCoV-OC43 replication in the central nervous system, and provide effective protection against HCoV-OC43 infection in mice with low drug toxicity. Furthermore, Ly-8-resistant mutants were not observed during the 30 times sequential passages in cell culture. Collectively, these findings suggest that Ly-8 may be a potential candidate drug for the future development of broad-spectrum anti-coronavirus drugs.</p></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 2","pages":"Pages 75-87"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949928323000330/pdfft?md5=95d7c2c767239b59433d058a102b0136&pid=1-s2.0-S2949928323000330-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138626007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into varicella-zoster virus assembly from the B- and C-capsid at near-atomic resolution structures 从近原子分辨率结构的 B 型和 C 型囊壳透视水痘-带状疱疹病毒的组装过程

hLife Pub Date : 2024-02-01 DOI: 10.1016/j.hlife.2023.10.007

Lei Cao , Nan Wang , Zhe Lv , Wenyuan Chen , Zhonghao Chen , Lifei Song , Xueyan Sha , Guiqiang Wang , Yaling Hu , Xiaojun Lian , Guoliang Cui , Jinyan Fan , Yaru Quan , Hongrong Liu , Hai Hou , Xiangxi Wang

{"title":"Insights into varicella-zoster virus assembly from the B- and C-capsid at near-atomic resolution structures","authors":"Lei Cao , Nan Wang , Zhe Lv , Wenyuan Chen , Zhonghao Chen , Lifei Song , Xueyan Sha , Guiqiang Wang , Yaling Hu , Xiaojun Lian , Guoliang Cui , Jinyan Fan , Yaru Quan , Hongrong Liu , Hai Hou , Xiangxi Wang","doi":"10.1016/j.hlife.2023.10.007","DOIUrl":"10.1016/j.hlife.2023.10.007","url":null,"abstract":"<div><p>Varicella-zoster is a highly communicable virus that can be transmitted through the airborne route. About one quarter of people are infected with this virus. Previous studies have described the structure of A-capsid and a blurred reconstruction of the C-capsid with icosahedral symmetry. In this study, we have determined the more precise detailed structures of the varicella-zoster virus (VZV) B- and C-capsid in icosahedral symmetry using a combination of block-based reconstruction and symmetry relaxation strategies. In addition, we are reporting structural details of the portal vertex reconstructions in five-fold symmetry and portal reconstructions in twelve-fold symmetry. The structures unveil the basis for the high thermal stability of the VZV capsid. The conformational flexibility of structural elements of the capsid plays a role in the assembly of the capsid and drives processes critical for the viral life cycle. The results of the study open up new avenues for the development of drugs against a highly prevalent and contagious pathogen.</p></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 2","pages":"Pages 64-74"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949928323000196/pdfft?md5=e8d2965c1f6ce20c4006daafb54519ec&pid=1-s2.0-S2949928323000196-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136159593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the pathogenic, genetic and immunological studies of leprosy 麻风病病原学、遗传学和免疫学研究进展

hLife Pub Date : 2024-01-01 DOI: 10.1016/j.hlife.2023.10.003

Zihao Mi, Hong Liu, Furen Zhang

{"title":"Advances in the pathogenic, genetic and immunological studies of leprosy","authors":"Zihao Mi, Hong Liu, Furen Zhang","doi":"10.1016/j.hlife.2023.10.003","DOIUrl":"10.1016/j.hlife.2023.10.003","url":null,"abstract":"<div><p>Leprosy is an infectious granulomatous disease caused by <em>Mycobacterium leprae</em> that affects the skin and can lead to deformity by damaging peripheral nerves. Although leprosy is no longer an incurable disease, its epidemic has not been well controlled because of its unclear routes of transmission and the lack of an effective vaccine. Moreover, leprosy has long been an ideal disease model for the study of genetics and immunology of infectious diseases due to its strong genetic predisposition and immune-dependent spectrum of clinical manifestations. Here, we review the latest and important findings of the pathogenesis of leprosy. Recent studies have shown that the highly conserved <em>M. leprae</em> is zoonotic, which further complicates the ambiguous transmission of <em>M. leprae</em>. Genetically, genome-wide association studies of leprosy have reported dozens of susceptibility genes, most of which are immune-related, and thus systematically elucidate the immunogenetic basis of the disease. Immunologically, plenty of novel mechanisms of host defense against intracellular bacterial infection and the modulation of host immunity by <em>M. leprae</em> have been depicted. Despite these great achievements, there are still gaps between pathogenic biology, genetics, and immunology of leprosy, limiting our in-depth understanding of leprosy pathogenesis. Further efforts, such as multi-omics data integration and the development of viable animal models for immunogenetic studies are urgently needed to accelerate advances in the precise prevention and treatment of leprosy.</p></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 1","pages":"Pages 6-17"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949928323000159/pdfft?md5=a29e2ba2dee565c78023c3e3a118f9f0&pid=1-s2.0-S2949928323000159-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Medical Journals in China: From Wuyi Huijiang to Chinese Medical Journal and hLife 中国的医学期刊：从《武夷会刊》到《中华医学杂志》和《生命时报

hLife Pub Date : 2024-01-01 DOI: 10.1016/j.hlife.2023.08.001

Qun Yan , Yi Shi , George Fu Gao

引用次数: 0

Is the time right for systematically serotyping SARS-related coronaviruses? 对与 SARS 有关的冠状病毒进行系统血清分型的时机是否成熟？

hLife Pub Date : 2024-01-01 DOI: 10.1016/j.hlife.2023.12.007

Chee Wah Tan , Lin-Fa Wang

引用次数: 0

Crystal structures of RNA-dependent RNA polymerases from Jingmen tick virus and Alongshan virus 荆门蜱虫病毒和阿龙山病毒的 RNA 依赖性 RNA 聚合酶晶体结构

hLife Pub Date : 2024-01-01 DOI: 10.1016/j.hlife.2023.10.006

Zhenyang Liu , Qi Peng , Pu Han , Lu Kuai , Jianxun Qi , Yi Shi

{"title":"Crystal structures of RNA-dependent RNA polymerases from Jingmen tick virus and Alongshan virus","authors":"Zhenyang Liu , Qi Peng , Pu Han , Lu Kuai , Jianxun Qi , Yi Shi","doi":"10.1016/j.hlife.2023.10.006","DOIUrl":"10.1016/j.hlife.2023.10.006","url":null,"abstract":"<div><p>Jingmenviruses are a group of flavi-like viruses with segmented genome and have been found in various types of hosts, including humans, cattle, monkeys, bats, rodents, sheep, ticks, mosquitoes and nematodes. Jingmenviruses, including the Jingmen tick virus (JMTV) and Alongshan virus (ALSV), have been associated with febrile illness and flu-like symptoms in humans. Viral polymerase plays critical roles in genome replication and transcription and is an ideal target for antiviral drugs. Here, we determined the crystal structures of RNA-dependent RNA polymerase (RdRp) domains of JMTV and ALSV at 2.6 Å and 3.2 Å resolutions, respectively. The overall structures of JMTV and ALSV RdRp domains are similar to those from the typical unsegmented viruses in <em>Flaviviridae</em> family, especially the <em>Flavivirus</em> genus. JMTV and ALSV RdRps can be divided into three subdomains and the catalytical Motif A-G are conserved like the typical flaviviruses, whereas the zinc-binding pockets are absent from the JMTV and ALSV RdRps. The 5′-ends of jingmenvirus genomes are varied in length and sequence, and a highly conserved 8-nucleotide element located on the tip of stem loop A was identified and shown to be required for binding with RdRp and performing <em>de novo</em> replication activity. These findings provide important structural insights into RdRp of segmented flavivirus and reveal the key region of virus genome responsible for replication initiation, which would promote molecular understanding of segmented flavivirus replication and the structure-based design of antiviral drugs against flaviviruses.</p></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 1","pages":"Pages 18-31"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949928323000184/pdfft?md5=37ab197d096fad4e411d2c483b2dbbf9&pid=1-s2.0-S2949928323000184-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136169131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient inhibition of SARS-CoV-2 emerging EG.5, EG.5.1 and BA.2.86 variants by fusion inhibitor HY3000 peptide 融合抑制剂 HY3000 肽能有效抑制 SARS-CoV-2 新出现的 EG.5、EG.5.1 和 BA.2.86 变体

hLife Pub Date : 2024-01-01 DOI: 10.1016/j.hlife.2023.10.002

Lili Wu , Anqi Zheng , Yangming Tang , Xiaoyun Wang , Yue Gao , Wenwen Lei , Guizhen Wu , Qihui Wang , George Fu Gao

引用次数: 0