hLife最新文献_第7页

Phenazines: Natural products for microbial growth control 酚嗪类：控制微生物生长的天然产品

hLife Pub Date : 2024-03-01 DOI: 10.1016/j.hlife.2023.11.005

Cátia A Sousa , Marta Ribeiro , Francisca Vale , Manuel Simões

{"title":"Phenazines: Natural products for microbial growth control","authors":"Cátia A Sousa , Marta Ribeiro , Francisca Vale , Manuel Simões","doi":"10.1016/j.hlife.2023.11.005","DOIUrl":"10.1016/j.hlife.2023.11.005","url":null,"abstract":"<div><p>The unprecedented problem of antibiotic resistance has become a major challenge for public health, which has contributed to an increase in infections caused by such bacteria. These microbial infections, typically biofilm-related, are also coupled to an increase in human mortality and morbidity. However, the demand for new antimicrobial agents has increased along with the evolution of microbial resistance mechanisms. Natural products produced by bacteria, such as phenazines, have been recognized as an important source for the development of new antimicrobial agents, through the exploitation of their capacity to increase oxidative stress in other organisms. Phenazines are a large group of nitrogen-containing heterocyclic compounds and are essential for several cellular processes including iron acquisition, signaling events, enzymatic processes, and biofilm formation. Phenazine-inspired antibiotics (i.e., 2-bromo-1-hydroxyphenazine, 2,4-dibromo-1-hydroxyphenazine, bromophenazine-21, phenazine-13, and phenazine-14) are very active against a wide range of gram-positive and gram-negative bacteria, including those associated with severe infections. In this study, mechanisms of phenazine-inspired antibiotics in the cellular processes of planktonic and sessile bacteria are reviewed. Moreover, the application of phenazine-inspired antibiotics for the eradication of multidrug-resistant planktonic and biofilm bacterial infections is also reviewed.</p></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 3","pages":"Pages 100-112"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949928323000305/pdfft?md5=ca9b1d33f6fcf0a42282b66d3d76d57d&pid=1-s2.0-S2949928323000305-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139291409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety, immunogenicity, and preliminary efficacy of a randomized clinical trial of omicron XBB.1.5-containing bivalent mRNA vaccine 含 omicron XBB.1.5 双价 mRNA 疫苗随机临床试验的安全性、免疫原性和初步疗效

hLife Pub Date : 2024-03-01 DOI: 10.1016/j.hlife.2024.01.005

Xuanjing Yu , Wei Yang , Wei Li , Na Wan , Guanghong Yan , Zumi Zhou , Xiao Zhu , Wei Su , Yani Li , Chenyu Xing , Sifan Duan , Houze Yu , Xinshuai Zhao , Chunmei Li , Taicheng Zhou , Dingyun You , Jia Wei , Zijie Zhang

{"title":"Safety, immunogenicity, and preliminary efficacy of a randomized clinical trial of omicron XBB.1.5-containing bivalent mRNA vaccine","authors":"Xuanjing Yu , Wei Yang , Wei Li , Na Wan , Guanghong Yan , Zumi Zhou , Xiao Zhu , Wei Su , Yani Li , Chenyu Xing , Sifan Duan , Houze Yu , Xinshuai Zhao , Chunmei Li , Taicheng Zhou , Dingyun You , Jia Wei , Zijie Zhang","doi":"10.1016/j.hlife.2024.01.005","DOIUrl":"10.1016/j.hlife.2024.01.005","url":null,"abstract":"<div><p>Periodically updating coronavirus disease 19 (COVID-19) vaccines that offer broad-spectrum protection is needed given the strong immune evasion by the circulating omicron sublineages. The effectiveness of prototype and BA.4/5-containing bivalent mRNA vaccines is reduced when XBB subvariants predominate. We initiated an observer-blinded, three-arms study in 376 patients in Chinese individuals aged from 18 to 55 years old who had previously received three doses COVID-19 vaccine. Immunogenicity in terms of neutralizing antibodies elicited by a 30-μg dose of XBB.1.5-containing bivalent vaccine (RQ3027), a 30-μg dose of BA.2/BA.5-Alpha/Beta bivalent vaccine (RQ3025) and their precedent 30-μg Alpha/Beta (combined mutations) monovalent mRNA vaccine (RQ3013) and safety are primary and secondary endpoints, respectively. We recorded prescribed COVID-19 cases to explore the preliminary efficacy of three vaccines. RQ3027 and RQ3025 boosters elicited superior neutralizing antibodies (NAbs) against XBB.1.5, XBB.1.16, XBB.1.9.1, and JN.1 compared to RQ3013 at day 14 in participants without SARS-CoV-2 infection. All study vaccines were well-tolerated without serious adverse reactions identified. The incidence rates per 1000 person-years of COVID-19 cases during the 2nd-19th week after randomization were lowest in RQ3027. Overall, our data show that XBB.1.5-containing bivalent booster generated superior immunogenicity and better protection against newer severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants compared to BA.2/BA.5-containing bivalent and Alpha/Beta monovalent with no new safety concerns.</p></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 3","pages":"Pages 113-125"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949928324000075/pdfft?md5=1750ec45457d8ecf974579a3f73af535&pid=1-s2.0-S2949928324000075-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139687380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The structure of HSV-1 gB bound to a potent neutralizing antibody reveals a conservative antigenic domain across herpesviruses 与强效中和抗体结合的 HSV-1 gB 结构揭示了疱疹病毒的保守抗原结构域

hLife Pub Date : 2024-03-01 DOI: 10.1016/j.hlife.2023.12.004

Cong Sun , Jia-Wen Yang , Chu Xie , Xin-Yan Fang , Guo-Long Bu , Ge-Xin Zhao , Dan-Ling Dai , Zheng Liu , Mu-Sheng Zeng

引用次数: 0

Status and challenges of global antimicrobial resistance control: A dialogue between Professors Yonghong Xiao and Takeshi Nishijima 全球抗菌药耐药性控制的现状与挑战：肖永红教授与西岛武教授的对话

hLife Pub Date : 2024-02-01 DOI: 10.1016/j.hlife.2023.11.004

Yonghong Xiao , Takeshi Nishijima

引用次数: 0

Mucosal vaccine development for respiratory viral infections 针对呼吸道病毒感染的粘膜疫苗开发

hLife Pub Date : 2024-02-01 DOI: 10.1016/j.hlife.2023.12.005

Yifan Lin , Zhenxiang Hu , Yang-Xin Fu , Hua Peng

{"title":"Mucosal vaccine development for respiratory viral infections","authors":"Yifan Lin , Zhenxiang Hu , Yang-Xin Fu , Hua Peng","doi":"10.1016/j.hlife.2023.12.005","DOIUrl":"10.1016/j.hlife.2023.12.005","url":null,"abstract":"<div><p>Mucosal vaccines have risen to prominence in the corona virus disease 2019 (COVID-19) pandemic due to their ability to elicit both local antibody and tissue-resident T cell responses, affording a dual-layered defense against infection and transmission at respiratory entry sites. While intramuscular vaccines predominantly focus on systemic immunity, mucosal vaccines offer a more nuanced, site-specific approach. However, the field faces a dearth of mucosal vaccine options for respiratory diseases, starkly contrasting to the extensive array of well-characterized injectable vaccines. The unique features of mucosal surfaces necessitate specialized adjuvants and delivery systems, adding complexity to adapting injectable vaccine technologies for mucosal applications. Here, we review the recent insights into the specificities of respiratory mucosal immunology that provide a foundation for future innovations besides the emerging vaccine platforms, newly discovered adjuvants, and vaccine delivery systems, which may open promising avenues for developing mucosal vaccines targeting respiratory pathogens.</p></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 2","pages":"Pages 50-63"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949928323000378/pdfft?md5=8ffe7a7a18e2adb9d50bcdfca868c959&pid=1-s2.0-S2949928323000378-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139023278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative interactomic of CD40 in primary B cells 原代 B 细胞中 CD40 的定量交互组学

hLife Pub Date : 2024-02-01 DOI: 10.1016/j.hlife.2023.10.009

Jeremy Argenty , Emilie Maturin , Mylene Camus , Valentin Mellado , Jeanne Perroteau , Benoit Pasquier , Guillaume Voisinne , Odile Burlet-Schiltz , Lih-Ling Lin , Anne Gonzalez de Peredo , Romain Roncagalli , Bernard Malissen

引用次数: 0

Getting ready for the next inforuses 为下一次注入做好准备

hLife Pub Date : 2024-02-01 DOI: 10.1016/j.hlife.2024.01.001

André Costa Lobato

引用次数: 0

Lycorine derivative effectively inhibits the replication of coronaviruses both in vitro and in vivo 番茄红素衍生物能有效抑制冠状病毒在体外和体内的复制

hLife Pub Date : 2024-02-01 DOI: 10.1016/j.hlife.2023.12.001

Liang Shen , Jianzhong Zhao , Ying Xia , Junjie Lu , Jiali Sun , Jian Tang , Hui Xing , Lijuan Yin , Yang Yang , Chunhua Wang

{"title":"Lycorine derivative effectively inhibits the replication of coronaviruses both in vitro and in vivo","authors":"Liang Shen , Jianzhong Zhao , Ying Xia , Junjie Lu , Jiali Sun , Jian Tang , Hui Xing , Lijuan Yin , Yang Yang , Chunhua Wang","doi":"10.1016/j.hlife.2023.12.001","DOIUrl":"10.1016/j.hlife.2023.12.001","url":null,"abstract":"<div><p>The established and ongoing prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and seasonal human coronaviruses (HCoV) like HCoV-OC43, HCoV-NL63, and HCoV-229E, pose a continuous threat to public health. Therefore, it is urgently needed to explore antiviral drugs with broad-spectrum anti-coronavirus activity. Our previous studies have revealed that lycorine is a potent broad-spectrum anti-coronavirus drug, a natural alkaloid extracted from <em>Amaryllidaceae</em> with various pharmacological and microbiological effects. However, it is unsafe to directly use lycorine as a clinical antiviral drug due to the cytotoxicity and induction of cell apoptosis. In this study, a series of lycorine derivatives were designed and synthesized. One of them, named Ly-8, was found to effectively inhibit the replication of different coronavirus strains <em>in vitro</em>, including SARS-CoV-2. Moreover, Ly-8 was also shown to effectively inhibit HCoV-OC43 replication in the central nervous system, and provide effective protection against HCoV-OC43 infection in mice with low drug toxicity. Furthermore, Ly-8-resistant mutants were not observed during the 30 times sequential passages in cell culture. Collectively, these findings suggest that Ly-8 may be a potential candidate drug for the future development of broad-spectrum anti-coronavirus drugs.</p></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 2","pages":"Pages 75-87"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949928323000330/pdfft?md5=95d7c2c767239b59433d058a102b0136&pid=1-s2.0-S2949928323000330-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138626007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into varicella-zoster virus assembly from the B- and C-capsid at near-atomic resolution structures 从近原子分辨率结构的 B 型和 C 型囊壳透视水痘-带状疱疹病毒的组装过程

hLife Pub Date : 2024-02-01 DOI: 10.1016/j.hlife.2023.10.007

Lei Cao , Nan Wang , Zhe Lv , Wenyuan Chen , Zhonghao Chen , Lifei Song , Xueyan Sha , Guiqiang Wang , Yaling Hu , Xiaojun Lian , Guoliang Cui , Jinyan Fan , Yaru Quan , Hongrong Liu , Hai Hou , Xiangxi Wang

{"title":"Insights into varicella-zoster virus assembly from the B- and C-capsid at near-atomic resolution structures","authors":"Lei Cao , Nan Wang , Zhe Lv , Wenyuan Chen , Zhonghao Chen , Lifei Song , Xueyan Sha , Guiqiang Wang , Yaling Hu , Xiaojun Lian , Guoliang Cui , Jinyan Fan , Yaru Quan , Hongrong Liu , Hai Hou , Xiangxi Wang","doi":"10.1016/j.hlife.2023.10.007","DOIUrl":"10.1016/j.hlife.2023.10.007","url":null,"abstract":"<div><p>Varicella-zoster is a highly communicable virus that can be transmitted through the airborne route. About one quarter of people are infected with this virus. Previous studies have described the structure of A-capsid and a blurred reconstruction of the C-capsid with icosahedral symmetry. In this study, we have determined the more precise detailed structures of the varicella-zoster virus (VZV) B- and C-capsid in icosahedral symmetry using a combination of block-based reconstruction and symmetry relaxation strategies. In addition, we are reporting structural details of the portal vertex reconstructions in five-fold symmetry and portal reconstructions in twelve-fold symmetry. The structures unveil the basis for the high thermal stability of the VZV capsid. The conformational flexibility of structural elements of the capsid plays a role in the assembly of the capsid and drives processes critical for the viral life cycle. The results of the study open up new avenues for the development of drugs against a highly prevalent and contagious pathogen.</p></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 2","pages":"Pages 64-74"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949928323000196/pdfft?md5=e8d2965c1f6ce20c4006daafb54519ec&pid=1-s2.0-S2949928323000196-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136159593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the pathogenic, genetic and immunological studies of leprosy 麻风病病原学、遗传学和免疫学研究进展

hLife Pub Date : 2024-01-01 DOI: 10.1016/j.hlife.2023.10.003

Zihao Mi, Hong Liu, Furen Zhang

{"title":"Advances in the pathogenic, genetic and immunological studies of leprosy","authors":"Zihao Mi, Hong Liu, Furen Zhang","doi":"10.1016/j.hlife.2023.10.003","DOIUrl":"10.1016/j.hlife.2023.10.003","url":null,"abstract":"<div><p>Leprosy is an infectious granulomatous disease caused by <em>Mycobacterium leprae</em> that affects the skin and can lead to deformity by damaging peripheral nerves. Although leprosy is no longer an incurable disease, its epidemic has not been well controlled because of its unclear routes of transmission and the lack of an effective vaccine. Moreover, leprosy has long been an ideal disease model for the study of genetics and immunology of infectious diseases due to its strong genetic predisposition and immune-dependent spectrum of clinical manifestations. Here, we review the latest and important findings of the pathogenesis of leprosy. Recent studies have shown that the highly conserved <em>M. leprae</em> is zoonotic, which further complicates the ambiguous transmission of <em>M. leprae</em>. Genetically, genome-wide association studies of leprosy have reported dozens of susceptibility genes, most of which are immune-related, and thus systematically elucidate the immunogenetic basis of the disease. Immunologically, plenty of novel mechanisms of host defense against intracellular bacterial infection and the modulation of host immunity by <em>M. leprae</em> have been depicted. Despite these great achievements, there are still gaps between pathogenic biology, genetics, and immunology of leprosy, limiting our in-depth understanding of leprosy pathogenesis. Further efforts, such as multi-omics data integration and the development of viable animal models for immunogenetic studies are urgently needed to accelerate advances in the precise prevention and treatment of leprosy.</p></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 1","pages":"Pages 6-17"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949928323000159/pdfft?md5=a29e2ba2dee565c78023c3e3a118f9f0&pid=1-s2.0-S2949928323000159-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0