hLife最新文献

{"title":"PGRP-SC–mediated innate immune memory against pathogenic bacteria in Drosophila","authors":"Zihao Deng ,&nbsp;Jiazhen Luo ,&nbsp;Yufu Zhang ,&nbsp;Chen Li ,&nbsp;Shixin Deng ,&nbsp;Jiazhi He ,&nbsp;Zihao He ,&nbsp;Wenqi Wu ,&nbsp;Renjie Jiao ,&nbsp;Jiyong Liu","doi":"10.1016/j.hlife.2025.09.003","DOIUrl":"10.1016/j.hlife.2025.09.003","url":null,"abstract":"<div><div>Trained immunity is essential for innate immune cells to retain a memory of previously encountered pathogens, strengthening the hosts’ response against these pathogens. However, the mechanisms governing trained immunity have not been well elucidated. In this study, flies of different genotypes were trained with heat-killed gram-negative (G⁻) bacteria and subsequently reinfected with live pathogens. The innate immune responses against reinfection were evaluated through assessments of survival rates, antimicrobial peptide expression levels, and bacterial loads, complemented by transcriptomic and chromatin immunoprecipitation (ChIP) analyses. We found that flies trained with heat-killed gram-negative bacteria exhibited a higher survival rate upon secondary infection compared to unprimed flies, which was associated with increased expression of antimicrobial peptides. Priming with G⁻ bacteria increased the sensitivity of the immune deficiency pathway to a second bacterial infection owing to lower levels of peptidoglycan recognition protein SC (PGRP-SC) after the first infection. The gut was the major tissue involved in the downregulation of PGRP-SC expression. The histone H3 lysine 9 trimethylation (H3K9me3) levels were higher at the <em>PGRP-SC</em> loci in immune-trained flies compared to untrained flies, contributing to the suppression of PGRP-SC expression. PGRP-SC overexpression in the fly gut abolished the effect of trained immunity. Taken together, our studies identify an innate immune memory in <em>Drosophila</em> that is regulated by gut-derived PGRP-SC through H3K9me3-mediated epigenetic repression of the <em>PGRP-SC</em>.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"4 1","pages":"Pages 41-55"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145872154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations of twelve DNA methylation algorithms of aging with mortality","authors":"Hui Zhang ,&nbsp;Darong Hao ,&nbsp;Yulu Gong ,&nbsp;Yaqian Xu ,&nbsp;Chongyu Ding ,&nbsp;Jing Wang ,&nbsp;Tongyan An ,&nbsp;Yining Liu ,&nbsp;Qiming Yin ,&nbsp;Tianlang Tong ,&nbsp;Xiangwei Li","doi":"10.1016/j.hlife.2025.05.010","DOIUrl":"10.1016/j.hlife.2025.05.010","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"4 1","pages":"Pages 56-58"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145872155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing priority for antifungal resistance research—Are we making progress? An excerpt from GAMRIF summit 2025","authors":"Chibuike Ibe","doi":"10.1016/j.hlife.2025.09.004","DOIUrl":"10.1016/j.hlife.2025.09.004","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"4 1","pages":"Pages 59-62"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145872156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mighty oaks from little acorns: High-quality genomes of underrepresented populations enhance health equity in precision medicine","authors":"Mengge Wang ,&nbsp;Lintao Luo ,&nbsp;Hui-Yuan Yeh ,&nbsp;Chuan-Chao Wang ,&nbsp;Huijun Yuan ,&nbsp;Chao Liu ,&nbsp;Renkuan Tang ,&nbsp;Guanglin He","doi":"10.1016/j.hlife.2025.05.014","DOIUrl":"10.1016/j.hlife.2025.05.014","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 621-625"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell RNA sequencing profiles age-related transcriptional landscapes in human hair follicle cells","authors":"Qian Zhao ,&nbsp;Rui Ma ,&nbsp;Kun Huang ,&nbsp;Juan Wang ,&nbsp;Donglin Zhang ,&nbsp;Jingyuan Wang ,&nbsp;Xiaofeng Ding ,&nbsp;Feiyun Chen ,&nbsp;Sijia Zhao ,&nbsp;Na Ni ,&nbsp;Xiaodie Zhang ,&nbsp;Qian Du ,&nbsp;Xiaojun Lin ,&nbsp;Hua Wan ,&nbsp;Jianglin Zhang ,&nbsp;Xiaolei Ding ,&nbsp;Shuang Yang ,&nbsp;Fengping Xu ,&nbsp;Yongxian Lai","doi":"10.1016/j.hlife.2025.10.003","DOIUrl":"10.1016/j.hlife.2025.10.003","url":null,"abstract":"<div><div>Hair loss and graying, the earliest visible signs of skin aging, are driven by the functional decline of hair follicle stem cells and their niches. To elucidate the transcriptional mechanisms involved in scalp aging, we conducted a comprehensive analysis of human scalp samples using single-cell RNA sequencing and spatial transcriptomic technologies. Our study profiled the transcriptomes of 57,181 cells from scalp samples obtained from four young, six middle-aged, and one elderly individual. The integrated bioinformatic pipeline included cell clustering, spatial deconvolution, pseudotime trajectory, as well as cell-type specific gene expression, and intercellular communication analysis. An additional 92 volunteers were included, comprising 90 (37 young, 27 middle-aged, and 26 elderly) for trichoscopic examination, one young individual for senescence-associated β-galactosidase (SA-β-gal) staining, and one elderly individual for both MKI67 immunofluorescence and SA-β-gal staining. This approach led to several key findings: we identified three subtypes of mitotic keratinocytes that localized in the interfollicular epidermis (IFE), outer root sheath (ORS), and hair matrix, with pseudotime trajectory further confirming their transitional stage. Furthermore, in middle-aged scalps, we observed activated activator protein 1 (AP-1) transcription factor complex in keratinocytes, upregulated <em>DCT</em> gene in melanocytes, and decreased bone morphogenetic protein (BMP) and noncanonical wingless/integrated (ncWNT) signaling in dermal papilla (DP)–keratinocytes cross-talk. Due to the insufficient sample size and under-representation of elderly samples, transcriptional features associated with late aging, sex, and scalp regions were not completely captured. Nevertheless, our study provides valuable cell-resolved transcriptional insights into hair follicle aging and may support the development of future regenerative therapies.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 626-646"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHIKVdb: A comprehensive genomic resource for chikungunya virus surveillance and outbreak response","authors":"Chongye Guo ,&nbsp;Guomei Fan ,&nbsp;Qi Chen ,&nbsp;Shiwen Li ,&nbsp;Min Li ,&nbsp;Dongmei Liu ,&nbsp;Juncai Ma ,&nbsp;Linhuan Wu","doi":"10.1016/j.hlife.2025.09.001","DOIUrl":"10.1016/j.hlife.2025.09.001","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 647-650"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A One Health perspective on the 2025 chikungunya outbreak in Guangdong province, China","authors":"Cheng Guo ,&nbsp;Nischay Mishra ,&nbsp;Abdel Aouacheria ,&nbsp;Cong Tuan Pham ,&nbsp;Ernest Konadu Asiedu ,&nbsp;Yan Long ,&nbsp;George Fu Gao ,&nbsp;Jiahai Lu","doi":"10.1016/j.hlife.2025.09.007","DOIUrl":"10.1016/j.hlife.2025.09.007","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 618-620"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 2025 Nobel Prize in Physiology or Medicine: The Treg, FoxP3, and STAT-regulated immune network","authors":"Xin-Yuan Fu","doi":"10.1016/j.hlife.2025.10.004","DOIUrl":"10.1016/j.hlife.2025.10.004","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 573-575"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pancreatic cancer cachexia: A systemic consequence of multi-organ interactions","authors":"Aoyi Xiao ,&nbsp;Yingying Feng ,&nbsp;Bohui Yin ,&nbsp;Jingcheng Zhang ,&nbsp;Zhe Cao ,&nbsp;Xudong Liu ,&nbsp;Yanshan Liang ,&nbsp;Wenming Wu","doi":"10.1016/j.hlife.2025.05.002","DOIUrl":"10.1016/j.hlife.2025.05.002","url":null,"abstract":"<div><div>Pancreatic cancer cachexia is a complex, multifactorial syndrome characterized by progressive wasting of skeletal muscle and adipose tissue, contributing to poor prognosis and high mortality in pancreatic cancer patients. While muscle and fat loss are the hallmark features, pancreatic cancer cachexia is increasingly recognized as a systemic disorder involving extensive metabolic and inflammatory disruptions across multiple organs. Tumor-derived cachexia-inducing factors play a central role in driving systemic inflammation, metabolic dysregulation, and neuroendocrine abnormalities, leading to anorexia, gut dysbiosis, cardiac dysfunction, and pancreatic exocrine and endocrine insufficiency. These multi-organ disturbances form a vicious cycle that accelerates disease progression and complicates clinical management. In this review, we provide a comprehensive overview of pancreatic cancer cachexia, including its definitions, classification, and heterogeneous clinical presentations. We further examine recent findings on the molecular mediators of cachexia and their role in inter-organ communication networks. Additionally, we highlight advances in experimental models that enable the dissection of pancreatic cancer cachexia pathophysiology, and discuss emerging mechanism-based therapeutic strategies aimed at disrupting the cachexia cycle. A deeper understanding of the systemic nature of pancreatic cancer cachexia and the crosstalk among affected organs may inform the development of multi-targeted interventions and hold promise for improving patient outcomes.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 576-614"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-platform compatibility of a structure-guided chimeric mpox virus immunogen delivered via circular RNA delivery","authors":"Haoyi Ding ,&nbsp;Jiahao Wu ,&nbsp;Zhida Liu ,&nbsp;Chee Wah Tan ,&nbsp;Han Wang ,&nbsp;George Fu Gao","doi":"10.1016/j.hlife.2025.07.003","DOIUrl":"10.1016/j.hlife.2025.07.003","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 651-654"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}