hLife最新文献

{"title":"Single-cell RNA sequencing profiles age-related transcriptional landscapes in human hair follicle cells","authors":"Qian Zhao ,&nbsp;Rui Ma ,&nbsp;Kun Huang ,&nbsp;Juan Wang ,&nbsp;Donglin Zhang ,&nbsp;Jingyuan Wang ,&nbsp;Xiaofeng Ding ,&nbsp;Feiyun Chen ,&nbsp;Sijia Zhao ,&nbsp;Na Ni ,&nbsp;Xiaodie Zhang ,&nbsp;Qian Du ,&nbsp;Xiaojun Lin ,&nbsp;Hua Wan ,&nbsp;Jianglin Zhang ,&nbsp;Xiaolei Ding ,&nbsp;Shuang Yang ,&nbsp;Fengping Xu ,&nbsp;Yongxian Lai","doi":"10.1016/j.hlife.2025.10.003","DOIUrl":"10.1016/j.hlife.2025.10.003","url":null,"abstract":"<div><div>Hair loss and graying, the earliest visible signs of skin aging, are driven by the functional decline of hair follicle stem cells and their niches. To elucidate the transcriptional mechanisms involved in scalp aging, we conducted a comprehensive analysis of human scalp samples using single-cell RNA sequencing and spatial transcriptomic technologies. Our study profiled the transcriptomes of 57,181 cells from scalp samples obtained from four young, six middle-aged, and one elderly individual. The integrated bioinformatic pipeline included cell clustering, spatial deconvolution, pseudotime trajectory, as well as cell-type specific gene expression, and intercellular communication analysis. An additional 92 volunteers were included, comprising 90 (37 young, 27 middle-aged, and 26 elderly) for trichoscopic examination, one young individual for senescence-associated β-galactosidase (SA-β-gal) staining, and one elderly individual for both MKI67 immunofluorescence and SA-β-gal staining. This approach led to several key findings: we identified three subtypes of mitotic keratinocytes that localized in the interfollicular epidermis (IFE), outer root sheath (ORS), and hair matrix, with pseudotime trajectory further confirming their transitional stage. Furthermore, in middle-aged scalps, we observed activated activator protein 1 (AP-1) transcription factor complex in keratinocytes, upregulated <em>DCT</em> gene in melanocytes, and decreased bone morphogenetic protein (BMP) and noncanonical wingless/integrated (ncWNT) signaling in dermal papilla (DP)–keratinocytes cross-talk. Due to the insufficient sample size and under-representation of elderly samples, transcriptional features associated with late aging, sex, and scalp regions were not completely captured. Nevertheless, our study provides valuable cell-resolved transcriptional insights into hair follicle aging and may support the development of future regenerative therapies.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 626-646"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHIKVdb: A comprehensive genomic resource for chikungunya virus surveillance and outbreak response","authors":"Chongye Guo ,&nbsp;Guomei Fan ,&nbsp;Qi Chen ,&nbsp;Shiwen Li ,&nbsp;Min Li ,&nbsp;Dongmei Liu ,&nbsp;Juncai Ma ,&nbsp;Linhuan Wu","doi":"10.1016/j.hlife.2025.09.001","DOIUrl":"10.1016/j.hlife.2025.09.001","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 647-650"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A One Health perspective on the 2025 chikungunya outbreak in Guangdong province, China","authors":"Cheng Guo ,&nbsp;Nischay Mishra ,&nbsp;Abdel Aouacheria ,&nbsp;Cong Tuan Pham ,&nbsp;Ernest Konadu Asiedu ,&nbsp;Yan Long ,&nbsp;George Fu Gao ,&nbsp;Jiahai Lu","doi":"10.1016/j.hlife.2025.09.007","DOIUrl":"10.1016/j.hlife.2025.09.007","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 618-620"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 2025 Nobel Prize in Physiology or Medicine: The Treg, FoxP3, and STAT-regulated immune network","authors":"Xin-Yuan Fu","doi":"10.1016/j.hlife.2025.10.004","DOIUrl":"10.1016/j.hlife.2025.10.004","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 573-575"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pancreatic cancer cachexia: A systemic consequence of multi-organ interactions","authors":"Aoyi Xiao ,&nbsp;Yingying Feng ,&nbsp;Bohui Yin ,&nbsp;Jingcheng Zhang ,&nbsp;Zhe Cao ,&nbsp;Xudong Liu ,&nbsp;Yanshan Liang ,&nbsp;Wenming Wu","doi":"10.1016/j.hlife.2025.05.002","DOIUrl":"10.1016/j.hlife.2025.05.002","url":null,"abstract":"<div><div>Pancreatic cancer cachexia is a complex, multifactorial syndrome characterized by progressive wasting of skeletal muscle and adipose tissue, contributing to poor prognosis and high mortality in pancreatic cancer patients. While muscle and fat loss are the hallmark features, pancreatic cancer cachexia is increasingly recognized as a systemic disorder involving extensive metabolic and inflammatory disruptions across multiple organs. Tumor-derived cachexia-inducing factors play a central role in driving systemic inflammation, metabolic dysregulation, and neuroendocrine abnormalities, leading to anorexia, gut dysbiosis, cardiac dysfunction, and pancreatic exocrine and endocrine insufficiency. These multi-organ disturbances form a vicious cycle that accelerates disease progression and complicates clinical management. In this review, we provide a comprehensive overview of pancreatic cancer cachexia, including its definitions, classification, and heterogeneous clinical presentations. We further examine recent findings on the molecular mediators of cachexia and their role in inter-organ communication networks. Additionally, we highlight advances in experimental models that enable the dissection of pancreatic cancer cachexia pathophysiology, and discuss emerging mechanism-based therapeutic strategies aimed at disrupting the cachexia cycle. A deeper understanding of the systemic nature of pancreatic cancer cachexia and the crosstalk among affected organs may inform the development of multi-targeted interventions and hold promise for improving patient outcomes.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 576-614"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-platform compatibility of a structure-guided chimeric mpox virus immunogen delivered via circular RNA delivery","authors":"Haoyi Ding ,&nbsp;Jiahao Wu ,&nbsp;Zhida Liu ,&nbsp;Chee Wah Tan ,&nbsp;Han Wang ,&nbsp;George Fu Gao","doi":"10.1016/j.hlife.2025.07.003","DOIUrl":"10.1016/j.hlife.2025.07.003","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 651-654"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ten scientific questions on regional immunity in the pulmonary and respiratory mucosal systems","authors":"778th Xiangshan Science Conference Expert Panel","doi":"10.1016/j.hlife.2025.07.004","DOIUrl":"10.1016/j.hlife.2025.07.004","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 569-572"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ACE2-using coronaviruses: A global concern","authors":"Zepeng Xu ,&nbsp;André Costa Lobato ,&nbsp;Kefang Liu ,&nbsp;George Fu Gao","doi":"10.1016/j.hlife.2025.09.005","DOIUrl":"10.1016/j.hlife.2025.09.005","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 12","pages":"Pages 615-617"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145772007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From spectrum to species: A new era of medical microbiology with AI-powered Raman spectroscopy","authors":"Ziyi Zhang ,&nbsp;Yu Vincent Fu","doi":"10.1016/j.hlife.2025.06.004","DOIUrl":"10.1016/j.hlife.2025.06.004","url":null,"abstract":"","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 11","pages":"Pages 527-530"},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145529484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prophylactic and therapeutic itaconate treatment alleviates COVID-19-associated lung injury","authors":"Yao Jiang ,&nbsp;Peida Yang ,&nbsp;Bo Yao ,&nbsp;Liang Zhang ,&nbsp;Man Yang ,&nbsp;Yiyi Chen ,&nbsp;Lunzhi Yuan ,&nbsp;Junyu Chen ,&nbsp;Jinhang He ,&nbsp;Xing Lei ,&nbsp;Feng Chen ,&nbsp;Mingxi Yue ,&nbsp;Siping Yan ,&nbsp;Tong Cheng ,&nbsp;Yixin Chen ,&nbsp;Quan Yuan ,&nbsp;Jun Zhang ,&nbsp;Shuhai Lin ,&nbsp;Tianying Zhang ,&nbsp;Ningshao Xia","doi":"10.1016/j.hlife.2025.06.006","DOIUrl":"10.1016/j.hlife.2025.06.006","url":null,"abstract":"<div><div>Itaconate (ITA), an immunomodulatory metabolite with known anti-inflammatory properties, has underexplored therapeutic or prophylactic potential against coronavirus disease 2019 (COVID-19). Using an interanimal transmission golden hamster model of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-induced acute lung injury, we first assessed ITA changes in dNS1-RBD-vaccinated hamsters <em>via</em> metabolomic profiling. Then, we evaluated prophylactic intranasal (20 mg/kg at 9, 6, and 3 days before SARS-CoV-2 infection) and therapeutic intraperitoneal (100 mg/kg at 6, 24, and 48 hours post-infection) ITA administration, assessed by histopathology, transcriptomic, and metabolomic profiling, followed by multi-omics integration, including gene expression clustering, pathway enrichment, and cytokine/chemokine–metabolites correlation analyses. Public bronchoalveolar lavage fluid (BALF) single-cell RNA-sequencing (scRNA-seq) datasets from COVID-19 patients were re-analyzed to explore macrophage heterogeneity. Intranasal dNS1-RBD vaccine upregulated ITA levels, prompting further exploration of its immunomodulatory role. Both prophylactic and therapeutic ITA treatments significantly mitigated weight loss and improved lung pathology. Correlation analyses implied a potential regulatory crosstalk between fatty acid β-oxidation (FAO) and reduced inflammatory response. Re-analysis of BALF scRNA-seq dataset highlighted transcriptional networks involving <em>PPARG</em>, <em>RARA</em>, <em>BHLHE41</em>, <em>TCF7L2</em>, and <em>ESRRA</em>—genes linked to macrophage self-renewal and metabolic homeostasis, which appeared to be preserved in ITA-treated hamsters. These findings underscore ITA's role in modulating immunometabolic responses, particularly through FAO-driven macrophage reprogramming, to attenuate SARS-CoV-2-induced lung damage. Together, this study provides insights into host-directed therapies targeting metabolic reprogramming to mitigate COVID-19 severity.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"3 11","pages":"Pages 551-564"},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145529478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}