hLife Pub Date : 2024-12-01 DOI: 10.1016/j.hlife.2024.09.002

Chang Guo , Xin Chai , Maidaiti Baerlike , Yingping Liu , Yao Wang , Fei Shao , Qingrui Huang , Weiguo Zhang , Shan Cen , Yijie Dong , Yunlong Cao , Jinghua Yan , Xuyu Zhou , Zhaolin Hua , Baidong Hou

{"title":"Comparison of antigen-specific B cell responses reveals disparity in immunogenicity and memory B cell formation across COVID-19 vaccine platforms","authors":"Chang Guo , Xin Chai , Maidaiti Baerlike , Yingping Liu , Yao Wang , Fei Shao , Qingrui Huang , Weiguo Zhang , Shan Cen , Yijie Dong , Yunlong Cao , Jinghua Yan , Xuyu Zhou , Zhaolin Hua , Baidong Hou","doi":"10.1016/j.hlife.2024.09.002","DOIUrl":"10.1016/j.hlife.2024.09.002","url":null,"abstract":"<div><div>Various vaccine technologies have been employed in the coronavirus disease 2019 (COVID-19) vaccines, including whole inactivated virus (WIV), recombinant protein, mRNA, and nanoparticle vaccines. To elucidate the cellular mechanisms underlying the immune responses elicited by different vaccines, we examined and compared antigen-specific B cell responses targeting the receptor-binding domain (RBD) of the viral spike protein. We found that the nanoparticle vaccine pathogen-like antigens-RBD (PLA-RBD) and the mRNA vaccine demonstrated superior immunogenicity compared with the WIV vaccine and the RBD-dimer, a recombinant protein vaccine. Interestingly, the WIV vaccine contains toll-like receptor ligands that enhance IgG2a/c class-switching. For the mRNA vaccine, although it induces robust germinal center responses and T follicular helper (Tfh) cells, it has limited ability to induce memory B cells and long-lived plasma cells. These results indicate that vaccine formats significantly influence both the quantity and quality of immune responses, providing valuable insights for the future development of vaccines.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 12","pages":"Pages 625-640"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myeloid cell diversity: From fundamental biology to disease states 骨髓细胞多样性：从基础生物学到疾病状态

hLife Pub Date : 2024-12-01 DOI: 10.1016/j.hlife.2024.11.001

Iván Ballesteros , Lai Guan Ng , Daniela F. Quail , Andrés Hidalgo

引用次数: 0

WHO revised bacterial priority pathogens list to encourage global actions to combat AMR

hLife Pub Date : 2024-12-01 DOI: 10.1016/j.hlife.2024.10.003

Yingying Ma , Pan Chen , Yin Mo , Yonghong Xiao

引用次数: 0

De novo design of covalent bonding peptides for target protein 为目标蛋白质重新设计共价键肽

hLife Pub Date : 2024-12-01 DOI: 10.1016/j.hlife.2024.07.007

Xiaohong Zhou , Qian Zhu , Anqi Zheng , Boyuan Xue , Qihui Wang , Lip Ket Chin , Jingkun Jiang , Miao He

{"title":"De novo design of covalent bonding peptides for target protein","authors":"Xiaohong Zhou , Qian Zhu , Anqi Zheng , Boyuan Xue , Qihui Wang , Lip Ket Chin , Jingkun Jiang , Miao He","doi":"10.1016/j.hlife.2024.07.007","DOIUrl":"10.1016/j.hlife.2024.07.007","url":null,"abstract":"<div><div>To rapidly develop hyper-stable inhibitors that bind specifically and covalently to functional proteins is critical for diagnostics and therapeutics. Taking targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants as an example, we report a fast and low-cost <em>de novo</em> design strategy on covalent bonding peptides toward the SARS-CoV-2 spike protein receptor-binding domain (RBD), hence blocking its interaction with the human angiotensin-converting enzyme 2 (hACE2). As a proof-of-concept, peptide scaffolds built by ligating the hotspot residues at the hACE2-Omicron RBD interface were docked against RBD, and then the chemically modified peptides were designed by predicting their reactivity against RBD using a modified Amber ff14SB force field. Two peptides (15- and 16-mer peptides) were equipped with sulfonyl fluoride warheads bound with the conserved Y449 residue of RBD <em>via</em> the sulfur (VI) fluoride exchange (SuFEx) click chemistry. With permanent bonding and without dissociation, the two peptides blocked Omicron BA.2 pseudovirus infection with 50% inhibitory concentration (IC<sub>50</sub>) values of 1.07 μM and 1.56 μM, respectively. Our design approach greatly promotes the discovery of hyper-stable inhibitors against SARS-CoV-2 variants and other rapidly evolving viruses, potentially applicable to combat future viral outbreaks efficiently.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 12","pages":"Pages 641-652"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding carbapenem-resistant hypervirulent Klebsiella pneumoniae: Key virulence factors and evolutionary convergence

hLife Pub Date : 2024-12-01 DOI: 10.1016/j.hlife.2024.06.005

Tao Chen , Liya Ying , Luying Xiong , Xueting Wang , Ping Lu , Yuan Wang , Ping Shen , Yonghong Xiao

{"title":"Understanding carbapenem-resistant hypervirulent Klebsiella pneumoniae: Key virulence factors and evolutionary convergence","authors":"Tao Chen , Liya Ying , Luying Xiong , Xueting Wang , Ping Lu , Yuan Wang , Ping Shen , Yonghong Xiao","doi":"10.1016/j.hlife.2024.06.005","DOIUrl":"10.1016/j.hlife.2024.06.005","url":null,"abstract":"<div><div>The emergence of hypervirulence (hv) and carbapenem resistance (CR) as distinct evolutionary directions for <em>Klebsiella pneumoniae</em> presents a significant threat in clinical settings. However, in recent years, there has been a growing identification of <em>K. pneumoniae</em> strains that integrate both phenotypes, resulting in severe clinical outcomes. Carbapenem-resistant hypervirulent <em>K. pneumoniae</em> (CRhvKP) typically emerges through the acquisition of plasmids carrying either virulence or CR-encoded genes by carbapenem-resistant <em>K. pneumoniae</em> or hypervirulent <em>K. pneumoniae</em>. Furthermore, the acquisition of a hybrid plasmid can confer a combination of CR and hv. CRhvKP can cause a variety of infections, including pneumonia, urinary tract infections, bloodstream infections, liver abscesses, and other related conditions. While the sequence type 11 (ST11) dominates the majority of CRhvKP strains in China, the molecular factors responsible for the success of ST11 CRhvKP largely remain unknown. Here, we provide an overview of the current understanding of the variation and distribution of crucial virulence determinants, the mechanisms driving the merging of hv and CR, and the potential molecular factors influencing the epidemiological success of ST11 CRhvKP. This research aims to contribute to a comprehensive understanding of the complexities surrounding CRhvKP. It is imperative to underscore the development of combination therapies, precision medicine, and vaccine strategies as pivotal approaches in effectively combating CRhvKP. Considering the widespread prevalence of CRhvKP, a prioritized, multifaceted approach encompassing infection control, active surveillance, and the development of innovative therapeutics is essential.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 12","pages":"Pages 611-624"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum regarding previously published papers

hLife Pub Date : 2024-12-01 DOI: 10.1016/j.hlife.2024.09.004

引用次数: 0

High-resolution crystal structure of human coronavirus HKU1 receptor binding domain bound to TMPRSS2 receptor

hLife Pub Date : 2024-12-01 DOI: 10.1016/j.hlife.2024.09.005

Wei Wang , Jiayu Guan , Minjie Ren , Zehou Li , Weiwei Ji , Rong Chen , Ying Xu , Shuijun Zhang

引用次数: 0

Cooperation and innovation: An interview with Minister Ibrokhim Y. Abdurakhmonov and Professor Jinghua Cao 合作与创新：访伊布拉希姆-阿布杜拉赫莫诺夫部长和曹靖华教授

hLife Pub Date : 2024-11-01 DOI: 10.1016/j.hlife.2024.06.004

Qun Yan , Ibrokhim Yulchievich Abdurakhmonov , Jinghua Cao

引用次数: 0

Population genomics of Central Asian peoples unveil ancient Trans-Eurasian genetic admixture and cultural exchanges 中亚民族的人口基因组学揭示了古代跨欧亚大陆的基因混杂和文化交流

hLife Pub Date : 2024-11-01 DOI: 10.1016/j.hlife.2024.06.006

Guanglin He , Mengge Wang , Lintao Luo , Qiuxia Sun , Haibing Yuan , Hongliang Lv , Yuhang Feng , Xiaojun Liu , Jing Cheng , Fengxiao Bu , Maxat Zhabagin , Huijun Yuan , Chao Liu , Shuhua Xu

{"title":"Population genomics of Central Asian peoples unveil ancient Trans-Eurasian genetic admixture and cultural exchanges","authors":"Guanglin He , Mengge Wang , Lintao Luo , Qiuxia Sun , Haibing Yuan , Hongliang Lv , Yuhang Feng , Xiaojun Liu , Jing Cheng , Fengxiao Bu , Maxat Zhabagin , Huijun Yuan , Chao Liu , Shuhua Xu","doi":"10.1016/j.hlife.2024.06.006","DOIUrl":"10.1016/j.hlife.2024.06.006","url":null,"abstract":"<div><div>Central Asia, a crucible of prehistoric and historical Trans-Eurasian interactions, has been pivotal in shaping cultural exchanges, population dynamics, and genetic admixture. Recent insights from ancient DNA studies have shed light on the extensive population turnover within this region, encompassing a spectrum of groups from Paleolithic hunter-gatherers to Holocene herders and the nomadic pastoralist empires of historical times. The genomic analysis of ancient pathogens across the Eurasian steppe has further deepened our understanding of pathogen origins, clonal expansions, and the intricate processes of host-pathogen coevolution in relation to varying pathogen exposures and their spread. We consolidate the latest findings pertaining to the ancient human and pathogen genomes of Central Asia, elucidating their profound influence on the genomic tapestry of contemporary Central Asians. A notable gap in the current genomic databases for Central Asia is underscored, particularly within the scope of genomics-driven precision medicine. We stress the urgent need for the development of extensive, region-specific genomic resources that hold promise for revealing the genetic blueprints underlying human traits and diseases, refining polygenic scoring models for predictive medicine, and bolstering genomic research endeavors across Central Asia.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 11","pages":"Pages 554-562"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nervonic acid alleviates stroke and its associated poststroke depression behaviors 神经酸能缓解中风及其相关的中风后抑郁行为

hLife Pub Date : 2024-11-01 DOI: 10.1016/j.hlife.2024.08.001

Fengrong Zhang , Yong Fan , Nghi Van Phung , Boyang Ji , Junmiao Chen , Xingyue Xu , Fuli Li , Peifeng Ji , Hongjun Yang , Xianyu Li

{"title":"Nervonic acid alleviates stroke and its associated poststroke depression behaviors","authors":"Fengrong Zhang , Yong Fan , Nghi Van Phung , Boyang Ji , Junmiao Chen , Xingyue Xu , Fuli Li , Peifeng Ji , Hongjun Yang , Xianyu Li","doi":"10.1016/j.hlife.2024.08.001","DOIUrl":"10.1016/j.hlife.2024.08.001","url":null,"abstract":"<div><div>Nervonic acid (NA) is an important long-chain monounsaturated fatty acid found in mammalian nervous tissue. It has recently garnered research attention due to its therapeutic potential in treating psychiatric and neurodegenerative disorders. In this study, we investigated the efficacy of NA in treating ischemia/reperfusion and poststroke events in a rat model. Specifically, there was significant reduction in the infarct area, cell death, and neuronal swelling after NA treatment, and the improvement in cerebral blood flow was also observed on day five after middle cerebral artery occlusion. Moreover, NA treatment led to the upregulation of brain-derived neurotrophic factors and myelin basic protein genes. NA displayed improved effects on depressive-like behavior of rats by three validated assays—the sucrose preference test, open-field test, and forced swim test. Regarding the mechanism of action, direct supplementation of NA in the brain was observed. We also observed the indirect effects of NA on the gut microbiota. Notably, the NA group gradually restored the bacterial diversity and the EGb group exhibited no impact based on observed-out analysis. We found an increase in the abundance of <em>Blautia</em> and <em>Sutterella</em>, which participated in phenylalanine metabolism. The metabolomics of plasma and brain samples revealed a decrease in the levels of phenylalanine-based amino acids, which alleviated the inhibitory effects on glutamine metabolism and promoted the recovery and signaling transmission of neurons after stroke. Altogether, our findings suggest that NA can be a viable treatment option for alleviating stroke and its associated poststroke depressive-like behaviors.</div></div>","PeriodicalId":100609,"journal":{"name":"hLife","volume":"2 11","pages":"Pages 592-606"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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