Carbohydrate Polymer Technologies and Applications最新文献_第4页

Chitosan powders and Ortho-R, a chitosan-containing finished product, are stable long term when stored in proper conditions

IF 6.2

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-03-21 DOI: 10.1016/j.carpta.2025.100777

Anik Chevrier , Dong Wang , Elizabeth Ladd , Jean-Simon Blais , Marc Lavertu

{"title":"Chitosan powders and Ortho-R, a chitosan-containing finished product, are stable long term when stored in proper conditions","authors":"Anik Chevrier , Dong Wang , Elizabeth Ladd , Jean-Simon Blais , Marc Lavertu","doi":"10.1016/j.carpta.2025.100777","DOIUrl":"10.1016/j.carpta.2025.100777","url":null,"abstract":"<div><div>Chitosan has sometimes been perceived to have a poor stability, which may explain why only a few chitosan-based products are available commercially. The purpose of this study was to investigate the stability of two chitosan powders and of Ortho-R (ChitogenX Inc), a chitosan-containing finished product that is used as an adjunct to improve repair of soft tissues. The powders and Ortho-R product were subjected to accelerated (40 °C/75 % RH) and long-term (25 °C/60 % RH) stability testing. The chitosan number average molar mass (<em>M</em><sub>n</sub>), polydispersity index (PDI), degree of deacetylation (DDA) and water content did not change substantially throughout the stability testing period and remained within the established specifications. Forced degradation studies were also performed on the Ortho-R product by exposing it to light, heat, humidity, hydrochloric acid and hydrogen peroxide. Storing the Ortho-R product in an open vial under warm and humid conditions (40 °C/75 % RH) led to an increase in chitosan <em>M</em><sub>n</sub> and PDI. The Ortho-R product was sensitive to oxidation as revealed by a decrease in chitosan <em>M</em><sub>n</sub> and PDI. Chitosan powders and the Ortho-R finished product are stable in the long term when the proper container closure systems are used.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100777"},"PeriodicalIF":6.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, fabrication and characterization of composite scaffolds employing sodium alginate, nanoclay and hydroxyapatite for articular cartilage tissue engineering

IF 6.2

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-03-21 DOI: 10.1016/j.carpta.2025.100771

Padideh Shahbazi , Majid Abdouss , Mitra Naeimi , Goldis Darbemamieh , Milad Jafari-Nodoushan

{"title":"Synthesis, fabrication and characterization of composite scaffolds employing sodium alginate, nanoclay and hydroxyapatite for articular cartilage tissue engineering","authors":"Padideh Shahbazi , Majid Abdouss , Mitra Naeimi , Goldis Darbemamieh , Milad Jafari-Nodoushan","doi":"10.1016/j.carpta.2025.100771","DOIUrl":"10.1016/j.carpta.2025.100771","url":null,"abstract":"<div><div>In this paper, composite scaffolds consisting of sodium alginate (SA), nanoclay (NC) and hydroxyapatite (HA) were fabricated for articular cartilage tissue engineering using freeze-drying method. Central composite design (CCD)-based response surface methodology (RSM) was used to anticipate the mechanical characteristics (in both compressive and tensile tests) and <em>in vitro</em> degradation of scaffolds with different amounts of SA and NC. The maximum tensile and compressive moduli (10.5 and 0.99 MPa, respectively) were found for the scaffold containing 2 g SA, 0.02 g NC and 0.2 g HA. In addition, it was found this composition had a relatively low degradation rate. Moreover, the optimized composition (S2N0.02) displayed a favorable porosity (50 %) and a proper swelling behavior. FTIR confirmed the successful integration of SA, NC and HA by presenting characteristic peaks corresponding to their functional groups, and SEM images showed the homogeneous dispersion of nanoparticles in the polymer matrix and the formation of interconnected pores suitable for cell seeding. Furthermore, MTT assay corroborated the good biocompatibility of the scaffolds for human chondrocytes. Drug release study showed that the scaffolds could effectively load glucosamine and provide its sustained release. These results confirm that SA/NC/HA composite scaffolds possess desirable properties for articular cartilage tissue engineering applications.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100771"},"PeriodicalIF":6.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CAZyme analysis and functional characterization of a new GH18 chitin hydrolase from Gelidibacter salicanalis PAMC21136

IF 6.2

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-03-21 DOI: 10.1016/j.carpta.2025.100773

Lakshan Paudel , Bashu Dev Pardhe , So-Ra Han , Jun Hyuck Lee , Tae-Jin Oh

{"title":"CAZyme analysis and functional characterization of a new GH18 chitin hydrolase from Gelidibacter salicanalis PAMC21136","authors":"Lakshan Paudel , Bashu Dev Pardhe , So-Ra Han , Jun Hyuck Lee , Tae-Jin Oh","doi":"10.1016/j.carpta.2025.100773","DOIUrl":"10.1016/j.carpta.2025.100773","url":null,"abstract":"<div><div><em>Gelidibacter salicanalis</em> strains remain poorly understood in terms of their genomic attributes and are rarely reported for the degradation of polysaccharides within the niche. <em>Gelidibacter salicanalis</em> PAMC21136, an Antarctic isolate, was sequenced and functionally annotated. 251 genes were classified into the CAZyme families, which includes the highest number of GH family, highlighting peculiarity towards various macro biopolymers. Further, based on domain architecture and multiple sequence alignment, gene belonging to the family18 glycoside hydrolase was identified as chitinase. The gene encoding chitinase (MBJ7879808.1) was successfully cloned and expressed in <em>Escherichia coli</em> BL21 cells. MBJ7879808.1 demonstrated activity towards colloidal chitin but there was no detectable activity towards microcrystalline crab shell chitin. Enzyme exhibited an exo-pattern of hydrolysis on both colloidal chitin, and shorter chain GlcNAc oligomers namely [(GlcNAc)<sub>3</sub>, (GlcNAc)<sub>4,</sub> (GlcNAc)<sub>5</sub>, and (GlcNAc)<sub>6</sub>] producing GlcNAc as a single final product. Biochemical characterization revealed an optimum activity at 25 °C and pH 7.0. Mn²⁺ ions significantly enhanced the activity by 1.7-fold. Turnover number (<em>K<sub>cat</sub></em>) was 8.6 min<sup>−1</sup> and catalytic efficiency (<em>K</em><sub>cat</sub>/<em>K</em><sub>m</sub>) was 2.0ml/mg/min towards the substrate colloidal chitin. These findings expanded the polysaccharide degradation capability of <em>Gelidibacter salicanalis</em> PAMC21136 and highlight the biotechnological potential of the GH18 enzyme towards production of chitooligomers.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100773"},"PeriodicalIF":6.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking finasteride's potential via carboxymethyl-β-cyclodextrin inclusion complex for androgenic alopecia

IF 6.2

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-03-21 DOI: 10.1016/j.carpta.2025.100767

Reza Allahyari , Behnam Mahdavi , Esmail Rezaei-Seresht , Mehdi Baghayeri

{"title":"Unlocking finasteride's potential via carboxymethyl-β-cyclodextrin inclusion complex for androgenic alopecia","authors":"Reza Allahyari , Behnam Mahdavi , Esmail Rezaei-Seresht , Mehdi Baghayeri","doi":"10.1016/j.carpta.2025.100767","DOIUrl":"10.1016/j.carpta.2025.100767","url":null,"abstract":"<div><div>This study focused on developing a carboxylated β-cyclodextrin (β-CD) formulation to enhance the physicochemical properties of finasteride by forming an inclusion complex. The goal was to evaluate its encapsulation efficiency, kinetic behavior, and thermodynamic properties thoroughly. For this purpose, β-CD was modified with carboxymethyl groups. Finasteride was then loaded into the carrier to prepare an inclusion complex (CM-β-CD/Fin). Various techniques were employed to characterize the product. Additionally, the stability, solubility, encapsulation efficiency (EE %), drug release profile, and cytotoxicity of CM-β-CD/Fin were investigated. The kinetic parameters and thermodynamic behavior of CM-β-CD/Fin were evaluated. XRD and FT-IR confirmed the successful formation of CM-β-CD/Fin. DLS analysis revealed a size distribution below 100 nm. FE-SEM showed a spherical morphology for CM-β-CD/Fin. The loading efficiency was approximately 85 %. The highest observed release was around 80 %, indicating potential for effective therapeutic use. The kinetic study of drug release indicated a non-Fickian mechanism. The thermodynamic evaluation suggested a spontaneous binding process along with an exothermic encapsulation reaction. Overall, the CM-β-CD/Fin combination exhibits advantageous characteristics suitable for controlled drug release systems. The results of this study are significant because the encapsulation of hydrophobic drugs like Fin can be challenging, and the method may provide better drug delivery properties.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100767"},"PeriodicalIF":6.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of hydrolysis agitation and suspension drying temperature on the synthesis of crystalline cellulose from jute fiber

IF 6.2

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-03-20 DOI: 10.1016/j.carpta.2025.100769

Md. Hasibul Hasan , Shakhawat Hossain , Md. Latifur Rahman , G. M. Shafiur Rahman , Mubarak A. Khan , Muhammad Abdullah Al Mamun

{"title":"Effect of hydrolysis agitation and suspension drying temperature on the synthesis of crystalline cellulose from jute fiber","authors":"Md. Hasibul Hasan , Shakhawat Hossain , Md. Latifur Rahman , G. M. Shafiur Rahman , Mubarak A. Khan , Muhammad Abdullah Al Mamun","doi":"10.1016/j.carpta.2025.100769","DOIUrl":"10.1016/j.carpta.2025.100769","url":null,"abstract":"<div><div>Proper synthesis mechanism and control over the synthesis process are essential for obtaining high-purity crystalline celluloses (CCs). In this research, CCs were synthesized from jute-derived cellulose through an acid hydrolysis process. The objective of the study was to analyze the effects of the hydrolysis stirring rate and suspension drying temperature on the crystallinity, crystal size and nature of CCs. FTIR analysis confirmed the optimal condition for extracting cellulose free from hemicellulose and lignin, that is for C<sub>3</sub> (cellulose extracted by 18 % NaOH treatment) and also provided evidence of crystallinity and purity of cellulose. Higher alkali affected the yield of cellulose negatively. At a fixed hydrolysis concentration of 50 %, continuous agitation resulted in cellulose nanocrystals (CNCs), while occasional agitation produced cellulose microfibers (CMFs). XRD analysis revealed that CNCs exhibited higher crystallinity than CMFs, with CNC<sub>1</sub> achieving the highest crystallinity of approximately 62.5 %. The SEM confirmed the morphology and dimensions of cellulose, CNCs and CMF, also the agglomeration of CNCs due to their moisture affinity. The drying temperature significantly influenced crystal size, with larger crystals forming at higher temperature (70 °C). TGA-DSC-DTG analyses indicated that, the thermal stability of CCs was somehow compromised, however, CCs retained a higher residue than cellulose at 550 °C.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100769"},"PeriodicalIF":6.2,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of trisodium citrate on the gelation properties of casein/gellan gum double gels formed at different temperature

IF 6.2

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-03-18 DOI: 10.1016/j.carpta.2025.100768

Weibo Zhang , Liang Zhao , Jing Wang , Yao Hu , Yi Wang , Pengjie Wang , Jiaqi Su , Hao Zhang , Ran Wang , Fazheng Ren , Chong Chen

{"title":"Effect of trisodium citrate on the gelation properties of casein/gellan gum double gels formed at different temperature","authors":"Weibo Zhang , Liang Zhao , Jing Wang , Yao Hu , Yi Wang , Pengjie Wang , Jiaqi Su , Hao Zhang , Ran Wang , Fazheng Ren , Chong Chen","doi":"10.1016/j.carpta.2025.100768","DOIUrl":"10.1016/j.carpta.2025.100768","url":null,"abstract":"<div><div>Gellan gum (GG) is widely used in the dairy products to enhance stability of casein (CN)-based gel by forming double gels. Ca<sup>2+</sup> is reported to facilitate GG gel formation, however, the effect of Ca<sup>2+</sup> on the gelation properties of CN/GG double gels remains unknown. In the study, the behavior of Ca<sup>2+</sup>was regulated by trisodium citrate (TC). TC decreased the water-holding capacity (WHC) and storage modulus (G') of CN/GG gels formed at 37℃. Whereas CN/GG gels with 30 mmol/L TC formed at 42℃ (42–30) exhibited the highest values of WHC and G' among that with 0–15 mmol/L TC. These results suggested TC had different effect on the gelation properties of the CN/GG gels formed at 37℃ and 42℃ with different gelation sequence. Mechanically, TC did not change the gelation sequence of CN/GG gels, which increased the free water content and caused larger void in CN/GG gels formed at 37℃, resulting in decreased WHC and G'. While TC-induced larger void slightly destroyed the continuity of CN gel in 42–30 sample and the addition of TC enhanced the individual CN gel, leading to higher WHC and G′. Overall, these findings demonstrated that TC's effect on CN/GG gels at 37℃ and 42℃ was contrary, which provided substantial basis for revealing the gelation mechanism of CN-based double gels.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100768"},"PeriodicalIF":6.2,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and characterization of alginate-based capsules containing lavender essential oil for acne therapy

IF 6.2

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-03-17 DOI: 10.1016/j.carpta.2025.100766

Yasmin Kabalan , Héloïse Bernarda , Xavier Montané , Silvia De la Flor , Bartosz Tylkowski , Anna Trojanowska , Annalisa La Gatta , Marta Giamberini

{"title":"Preparation and characterization of alginate-based capsules containing lavender essential oil for acne therapy","authors":"Yasmin Kabalan , Héloïse Bernarda , Xavier Montané , Silvia De la Flor , Bartosz Tylkowski , Anna Trojanowska , Annalisa La Gatta , Marta Giamberini","doi":"10.1016/j.carpta.2025.100766","DOIUrl":"10.1016/j.carpta.2025.100766","url":null,"abstract":"<div><div>In this study, we prepared alginate-based capsules containing lavender essential oil for cosmetic applications and acne treatment. To tune their preparation, several parameters have been changed to obtain empty and oil-containing beads, i.e. the alginate molecular weight and concentration and the cation concentration. Calcium-crosslinked beads were also studied as a reference system. The capsules were characterized by Optical Microscopy (OM), Environmental Scanning Electron Microscopy (ESEM), Thermogravimetric Analysis (TGA) and Dynamic Mechanical Analysis (DMA), while Encapsulation Efficiency was calculated by Gas Chromatography-Mass Spectrometry (GC–MS). Significant differences in the morphology of empty capsules are observed depending on the concentration of cation used and of the alginate solution; differently, capsules containing oil, regardless of the cation used, have a smooth surface and a highly porous interior. Encapsulation efficiency was very good, averaging 98 % for all samples. As for the mechanical properties of the capsules, the Young Modulus and the force at break were determined for oily and empty capsules with both cations. The empty capsules resulted more rigid and resistant than the oil-containing ones, while the latter showed an elastoplastic behaviour.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100766"},"PeriodicalIF":6.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural characterization and bioactivities of a glucomannan-based polysaccharide (AGP-1) from Arundina graminifolia

IF 6.2

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-03-17 DOI: 10.1016/j.carpta.2025.100758

Tianhao Dong , Huijuan Wang , Xuemei Lin , Wenzhong Zhao , Kunfa Yang , Fengxi Yang , Genfa Zhu , Jie Gao , Meifeng Liu

{"title":"Structural characterization and bioactivities of a glucomannan-based polysaccharide (AGP-1) from Arundina graminifolia","authors":"Tianhao Dong , Huijuan Wang , Xuemei Lin , Wenzhong Zhao , Kunfa Yang , Fengxi Yang , Genfa Zhu , Jie Gao , Meifeng Liu","doi":"10.1016/j.carpta.2025.100758","DOIUrl":"10.1016/j.carpta.2025.100758","url":null,"abstract":"<div><div>This work aims to explore and characterize the functional polysaccharide of <em>Arundina graminifolia</em>. The extraction process of the crude <em>Arundina graminifolia</em> polysaccharides (AGP) was optimized using response surface methodology (RSM). The maximum yield of AGP reached 10.2 % under the optimal conditions of extracting 84.7 min at 96.0 °C, and liquid-material ratio of 27.5 mL/g. Meanwhile, AGP-1, the purified polysaccharide, was obtained using DEAE-Cellulose52 and Sephadex G-200 chromatography, and its structure was analyzed and confirmed by multiple chromatographic and spectroscopic techniques. It was indicated that AGP-1 (Mw 494.3 kDa) was a glucomannan-based polysaccharide mainly consisted of mannose, glucose and galactose at a molar ratio of 10:7:1, and its structure skeleton contained glycosyl residues of Glc<em>p</em>-(1→, →4)-Man<em>p</em>-(1→, →4)-Glc<em>p</em>-(1→, →3,4)-Gal<em>p</em>-(1→ and →4,6)-Gal<em>p</em>-(1→. Distinct from the β-1,4 glycosidic linkage bonds of other glucomannans, AGP-1 was infrequently linked by α-1,4 glycosidic bonds between monosaccharides of mannose and glucose with a specific molar ratio of 3:2. Furthermore, it was found that AGP-1 exhibited strong DPPH, ABTS, and hydroxyl radicals scavenging abilities, along with excellent inhibition of α-glucosidase activity in vitro. Our finding indicated that AGP-1 possessed a potential as natural antioxidant and hypoglycemic product.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100758"},"PeriodicalIF":6.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spray-dried probiotic microcapsules with calcium cross-linked oxidized starch and inulin

IF 6.2

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-03-17 DOI: 10.1016/j.carpta.2025.100760

Mehdi Ahmadi , Fatemeh Khajeh , Sahand Sohrabi , Ramin Shahbahrami , Leila Mirmoghtadaie , Farzaneh Shahraz , Fereshte Bahmanyar , Fataneh Hashempour-Baltork , Seyede Marzieh Hosseini

{"title":"Spray-dried probiotic microcapsules with calcium cross-linked oxidized starch and inulin","authors":"Mehdi Ahmadi , Fatemeh Khajeh , Sahand Sohrabi , Ramin Shahbahrami , Leila Mirmoghtadaie , Farzaneh Shahraz , Fereshte Bahmanyar , Fataneh Hashempour-Baltork , Seyede Marzieh Hosseini","doi":"10.1016/j.carpta.2025.100760","DOIUrl":"10.1016/j.carpta.2025.100760","url":null,"abstract":"<div><div><em>Lactobacillus acidophilus</em> LA-5, a beneficial live microorganism requiring a minimum intake of 10<sup>6</sup> CFU for effective intestinal colonization, often suffers from poor stability in the harsh environment of gastrointestinal tract. This study explored the feasibility of using oxidized starch-inulin as a carrier material to enhance L. <em>acidophilus</em> LA-5 delivery and viability. The results demonstrated that Encapsulation with 1 % CaHPO4 (dicalcium phosphate) and 5 % inulin yielded the highest encapsulation efficiency (EE%) and yield (EY%). Inulin significantly enhanced probiotic survival in both SFG and, more importantly, in SIF probably through its providing nourishment for the probiotics and its potential protective effects against harsh stresses. An optimal concentration of 1 % CaHPO<sub>4</sub> in combination with inulin was essential for achieving better protection of the encapsulated bacteria, both in SFG and SIF (gastrointestinal conditions) environments. The addition of inulin modified the low-temperature endothermic point and enthalpy ranged from -146.4 J/g to -170.6 J/g, with higher values compared to samples without inulin. The thermal behavior of the microcapsules was also slightly influenced by the different concentrations of CaHPO<sub>4</sub>. The B2 microcapsules (1 % CaHPO<sub>4</sub>, 5 % oxidized starch, 5 % inulin) demonstrated the best probiotic survivability in long-term storage stability at all temperatures and the combination of inulin and CaHPO<sub>4</sub> provided synergistic protective effect against storage time and temperature. These findings indicate the promising application of oxidized starch-inulin and optimized CaHPO<sub>4</sub>/inulin combinations for enhancing probiotic delivery and efficacy.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100760"},"PeriodicalIF":6.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143685210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healing

IF 6.2

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-03-15 DOI: 10.1016/j.carpta.2025.100762

Urška Jančič , Isabella Nacu , Liliana Verestiuc , Fiorenza Rancan , Selestina Gorgieva

{"title":"Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healing","authors":"Urška Jančič , Isabella Nacu , Liliana Verestiuc , Fiorenza Rancan , Selestina Gorgieva","doi":"10.1016/j.carpta.2025.100762","DOIUrl":"10.1016/j.carpta.2025.100762","url":null,"abstract":"<div><div>Novel bioactive bacterial nanocellulose (BnC) membranes were developed for effective, non-surgical debridement and infection-prevention in burn wound healing. Membranes were modified <em>in situ</em> with carboxymethyl cellulose (CMC) and <em>ex situ</em> with the proteolytic enzyme bromelain (Br) and antimicrobial peptide nisin (N). Post-processing into stable cellulose nanocrystal dispersions (ζ = -26 mV), enables assembly of model films for demonstration of high, irreversible bromelain (95 %) and nisin (99.5 %) adsorption. The BnC-CMC and BnC-CMC-N membranes were <em>in vitro</em> cytocompatible for HaCaT cells and induced faster cell proliferation with cell viability exceeding 100 % after 24 h incubation. The innovative aspect of this study lies in the <em>ex vivo</em> evaluation using an advanced human skin explant model with induced burns, providing a realistic, physiologically relevant assessment of membrane performance. <em>Ex vivo</em> experiments indicated the cytocompatibility of the BnC-CMC membrane with no acute toxicity or skin irritation, while nisin presence resulted in moderate irritating effect. Notably, the BnC-CMC-Br membrane showed digestion of intercellular junctions in the epidermis, while not inducing acute toxicity and skin irritation. By leveraging this innovative <em>ex vivo</em> human skin model in novel BnC-based membranes testing, the study provides a crucial translational step, bridging <em>in vitro</em> assessments and clinical applications for burn wound treatment.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"10 ","pages":"Article 100762"},"PeriodicalIF":6.2,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0