Biomedical Technology最新文献

{"title":"HFF-Net: A hybrid convolutional neural network for diabetic retinopathy screening and grading","authors":"Muhammad Hassaan Ashraf ,&nbsp;Hamed Alghamdi","doi":"10.1016/j.bmt.2024.09.004","DOIUrl":"10.1016/j.bmt.2024.09.004","url":null,"abstract":"<div><div>Diabetic Retinopathy (DR) is a leading cause of vision loss among diabetic patients, necessitating effective screening and grading for timely intervention. Regular screening significantly increases the workload of ophthalmologists, and accurate grading into stages—mild, moderate, severe, and proliferative—is crucial for monitoring disease progression. While Computer-Aided Diagnosis (CAD) systems can alleviate this burden, existing Convolutional Neural Network (CNN)-based frameworks use fixed-size kernels in a linear feed-forward manner. This approach can lead to information loss in the initial stages due to limited feature utilization across adjacent layers. To address this limitation, we propose a Hierarchical Features Fusion Convolutional Neural Network (HFF-Net) within a Diabetic Retinopathy Screening and Grading (DRSG) framework. The framework includes preprocessing to extract regions of interest from fundus images (FIs), enhancement using Contrast Limited Adaptive Histogram Equalization (CLAHE), and data augmentation for class balancing and overfitting mitigation. HFF-Net extracts multiscale features that fused at multiple levels within the network, utilizing the swish activation function for improved learning stability. We evaluated HFF-Net against several state-of-the-art CNN classifiers within the DRSG framework. Experimental results demonstrate that HFF-Net achieves a grading accuracy of 73.77 ​%, surpassing the second-best model by 3.51 percentage points (a relative improvement of approximately 5 ​%), and attains a screening accuracy of 98.70 ​% using only 1.18 million parameters. These findings highlight HFF-Net's potential as an efficient and effective tool in CAD systems for DR screening and grading.</div><div>©2017 Elsevier Inc. All rights reserved.</div></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"8 ","pages":"Pages 50-64"},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterials that passively and actively target macrophages promote the regeneration of injured tissues","authors":"Pengzhen Zhuang,&nbsp;Wu Yang,&nbsp;Yu Chen,&nbsp;Yu Zhang,&nbsp;Capucine Leboucher,&nbsp;Jessica M. Rosenholm,&nbsp;Hongbo Zhang","doi":"10.1016/j.bmt.2024.09.005","DOIUrl":"10.1016/j.bmt.2024.09.005","url":null,"abstract":"<div><div>Modulation of the immune system has gained significant attention in regenerative medicine. Although most tissues possess intrinsic self-repair capabilities, large-sized defects and complex pathological conditions may still lead to tissue microenvironment imbalance and repair failure. Researchers have applied macrophage-mediated immunotherapeutic strategies to various injured tissue repairs by modulating inflammatory responses, intercellular communication, and multitissue synergies to restore immune microenvironmental homeostasis and promote tissue regeneration. Ongoing advancements in materials science have highlighted the precise immunomodulatory role of biomaterials, with passive targeting strategies based on the material's physicochemical properties and active targeting strategies based on specific molecular modifications becoming increasingly important in research. This review focuses on the mechanisms of action of actively and passively targeted biomaterials to modulate macrophages, which improve the tissue regenerative microenvironment through four basic strategies: (i) modulation of the inflammatory response to remove damaged cells, cellular debris, and pathogens; (ii) remodeling of the extracellular matrix; (iii) reconstruction of vascular tissues; and (iv) macrophage-stem cell crosstalk. This review covers the major mechanisms of macrophage action in the regeneration of injured tissues, while exploring the multiple methods by which advanced biomaterials target macrophages and highlighting their applications in the regeneration of a wide range of tissue injuries. We further discuss the future directions and current limitations in the development of biomaterials for macrophage modulation, aiming to advance biomaterials targeting macrophages, realize the full potential of immunotherapy, and achieve precision medicine.</div></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"8 ","pages":"Pages 17-49"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative exploration of deep convolutional neural networks using real-time endoscopy images","authors":"Subhashree Mohapatra ,&nbsp;Pukhraj Singh Jeji ,&nbsp;Girish Kumar Pati ,&nbsp;Manohar Mishra ,&nbsp;Tripti Swarnkar","doi":"10.1016/j.bmt.2024.09.003","DOIUrl":"10.1016/j.bmt.2024.09.003","url":null,"abstract":"<div><p>Until now various deep convolutional neural networks are designed and trained for the purpose of classifying different medical conditions related to the domain of gastroenterology. Most of the study carried out have considered publicly available datasets to train the classification networks. Nevertheless, the main motive for carrying out different works in the field gastroenterology is to administer the developed models in healthcare centers in real-world set-ups. For doing so, it is important to check the generalizing ability of the designed systems by regulating them so as to classify endoscopy images captured in a specific hospital. In this regard, the foremost work completed is the collection of the endoscopy data from the hospital and then correctly annotating the images taking the help of a senior endoscopist with experience of more than 5 years. Once the data annotation is completed, the images are segregated into the class of normal and abnormal endoscopy images. Four different models are designed in the current work based on deep learning models, transfer learning models and ensemble approaches, and trained to classify the hospital endoscopy data as normal or abnormal. The models are then tested and evaluated based on various performance measures. It is observed from the comparative analysis that the transfer learning-based ensemble model has the best generalizing ability and gives the best specificity of 100 ​%. It is believed that deep learning-based models can assist endoscopists in add-on to human prediction efficiency.</p></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"8 ","pages":"Pages 1-16"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949723X2400028X/pdfft?md5=e0845db8abb3c72b5f176a9ea7db3750&pid=1-s2.0-S2949723X2400028X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of orthotic insole on symptomatic flexible flatfoot in school-age children: Meta-analysis and 1-year follow-up study","authors":"Shuncai Hu,&nbsp;Qing Lin,&nbsp;Lifeng Qiu,&nbsp;Yang Liu,&nbsp;Siyan Guan,&nbsp;Zhizhi Luo,&nbsp;Yang Wang,&nbsp;Xiaofan Wang","doi":"10.1016/j.bmt.2024.08.001","DOIUrl":"10.1016/j.bmt.2024.08.001","url":null,"abstract":"<div><h3>Purpose</h3><p>This study aimed to investigate the efficacy of orthopedic insoles, specifically three-dimensional (3D)-printed orthopedic insoles, for treatment of symptomatic flexible flatfoot in school-age children.</p></div><div><h3>Methods</h3><p>A systematic review of PubMed and China National Knowledge Infrastructure (CNKI) from database inception to March 2024 was conducted to determine additional studies. This single-center study included 38 participants, including 20 who chose ordinary orthopedic insoles and 18 who chose 3D printed orthopedic insoles, presented from January 2021 to December 2022. Pain symptom relief was compared between the two groups after 1 year of follow-up.</p></div><div><h3>Results</h3><p>A systematic review identified an additional six publications, involving 206 samples, and meta-analysis indicated that the force-bearing area, arch index, and heel valgus angle after treatment were 0.74 (95 ​% confidence interval [CI]: 0.65–1.01), 0.20 (95 ​% CI: 0.03–1.35), and 0.10 (95 ​% CI: 0.03–0.28) of those before treatment, respectively. The 1-year follow-up study revealed that because of its good comfort, 3D printed orthopedic insole can significantly improve the wearing time of both male (<em>P</em> ​&lt; ​0.001) and overweight participants (<em>P</em> ​&lt; ​0.001) and significantly reduce the pain score (<em>P</em> ​= ​0.032).</p></div><div><h3>Conclusions</h3><p>Orthotic insoles are effective in helping the recovery of flexible flatfoot. Among them, the 3D-printed orthopedic insoles have a better effect on relieving pain symptoms and have a great development potential.</p></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"7 ","pages":"Pages 63-70"},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949723X24000163/pdfft?md5=b0f8149d222ae2bd36d56643a01112ba&pid=1-s2.0-S2949723X24000163-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging strategies for the treatment of endometriosis","authors":"Qiong Chen ,&nbsp;Jing Wang ,&nbsp;Xiaoya Ding ,&nbsp;Qingfei Zhang ,&nbsp;Ping Duan","doi":"10.1016/j.bmt.2024.08.002","DOIUrl":"10.1016/j.bmt.2024.08.002","url":null,"abstract":"<div><p>Endometriosis is an estrogen-dependent disorder of the reproductive tract, affecting approximately 10 ​% of women. The symptoms of this condition are vague and not correlated with the disease's stage. These associated symptoms significantly impact women's overall well-being. The etiology of endometriosis remains inadequately understood, with coelomic metaplasia, lymphatic and vascular dissemination being regarded as additional hypotheses in addition to the retrograde menstruation theory. Endometriosis is primarily treated with drug therapy and surgical intervention, but the recurrence rate of symptoms after five years remains approximately 50 ​%. Therefore, the advancement of more effective and safe therapies for the treatment of endometriosis is of paramount importance. In this review, we introduce the utilization of photodynamic therapy, hyperthermia, gene therapy, immunotherapy, stem cell therapy, nanotechnology, and micron technology in the management of endometriosis. The objective is to provide novel research perspectives for therapeutic approaches and facilitate future clinical translation to enhance patient outcomes.</p></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"7 ","pages":"Pages 46-62"},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949723X24000175/pdfft?md5=62b25ff0bb6b24e2e05534564539033a&pid=1-s2.0-S2949723X24000175-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-enhanced nature medicine for ischemic stroke: Opportunities and challenges","authors":"Jihao Yang ,&nbsp;Lingyi Guo ,&nbsp;Jun Liao ,&nbsp;Huaqiang Yi","doi":"10.1016/j.bmt.2024.07.001","DOIUrl":"10.1016/j.bmt.2024.07.001","url":null,"abstract":"<div><p>Ischemic stroke (IS), a major cause of death and disability globally, requires innovative therapeutic approaches due to its complex pathology. Nature medicine (NM) offers promising treatments through its bioactive compounds, which target the multifaceted nature of stroke-induced damage. However, the clinical application of NM is limited by challenges in bioavailability and specificity. This review article presents an advanced perspective on integrating nanotechnology with NM to create potent nanodelivery systems for ischemic stroke treatment. We highlight the pathological underpinnings of ischemic stroke, including oxidative stress, inflammation, and apoptosis, and discuss how NM compounds offer targeted mitigation strategies. By incorporating nanodelivery platforms, such as liposomes and nanoparticles, these NM -based treatments can achieve enhanced targeting, solubility, and controlled release, significantly improving therapeutic outcomes while reducing side effects. Despite promising developments, the translation of nano-enhanced NM into clinical practice faces obstacles, including manufacturing scalability, regulatory approval, and safety evaluations. This review emphasizes the potential of combining nanotechnology with NM to advance ischemic stroke therapy, calling for integrated research efforts to overcome existing barriers and fully realize the clinical benefits of this innovative approach.</p></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"7 ","pages":"Pages 32-45"},"PeriodicalIF":0.0,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949723X24000151/pdfft?md5=7ca6bcfb5188479a44a70cc5ccf9f911&pid=1-s2.0-S2949723X24000151-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141623405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The therapeutic efficacy of post-symptom 3,4-diaminopyridine treatment in cosmetic injection-induced botulism using a novel animal model","authors":"Ping He ,&nbsp;Rongshuai Yan ,&nbsp;Jie Liu ,&nbsp;Pan You ,&nbsp;Jianghe Zhang ,&nbsp;Jinqing Li ,&nbsp;Yiming Zhang","doi":"10.1016/j.bmt.2024.06.003","DOIUrl":"https://doi.org/10.1016/j.bmt.2024.06.003","url":null,"abstract":"<div><p>In recent years, the incidence of cosmetic injection-induced botulism has remarkably increased due to the frequent usage of botulinum neurotoxin type A (BoNT/A). To mimic and investigate this new clinical type of botulism, we established a novel animal model and evaluated the therapeutic potential of a new drug. Firstly, we injected BoNT/A into the gastrocnemius of rats to induce partial paralysis of the remaining limbs. Then, the intoxicated rats were treated with 3,4-diaminopyridine (3,4-DAP) at different stages of the disease and the forelimbs grasping strength (FGS) was evaluated. We showed that, at the sublethal dose, the FGS began to decrease at 6.00 ​± ​1.86 ​h after injection in rats, from 2.28 ​± ​0.19 ​N to 1.51 ​± ​0.18 ​N, while the FGS declined appeared earlier (4.29 ​± ​0.42 ​h) at the lethal dose, from 2.30 ​± ​0.20 ​N to 1.20 ​± ​0.16 ​N. Treatment with 3,4-DAP respectively at the time of the symptoms onset or 7 days after injection both can temporarily reverse the symptoms of muscle paralysis, indicating that 3,4-DAP may be an effective approach to relieve botulism. Overall, this study provides an available rat model and a promising therapeutic strategy for cosmetic injection-induced botulism.</p></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"7 ","pages":"Pages 25-31"},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949723X2400014X/pdfft?md5=33b378da876cfcc1ef62239719647f35&pid=1-s2.0-S2949723X2400014X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141540325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic insights into myopia development: Differential protein expression and the role of calcium signaling in form deprivation myopia in Guinea pigs","authors":"Rongbin Liang ,&nbsp;Tao Li ,&nbsp;Wenqing Shi ,&nbsp;Hui Gao ,&nbsp;Bei Ai ,&nbsp;Bing Li ,&nbsp;Xiaodong Zhou","doi":"10.1016/j.bmt.2024.06.001","DOIUrl":"https://doi.org/10.1016/j.bmt.2024.06.001","url":null,"abstract":"<div><p>This study aims to explore the changes in the vitreous proteomics of form deprivation myopia (FDM) in guinea pigs, in order to reveal the molecular mechanisms involved in the onset and development of myopia. The myopia model in guinea pigs was successfully established by covering one eye of the guinea pigs with a latex bead sac for 4 weeks. This study used 4D data-independent acquisition proteomics technology to analyze vitreous body samples from both the FDM group and the control group. The goal of the proteomics analysis was to identify differences in protein expression within the vitreous body of FDM guinea pigs. Myopia was successfully induced in the FDM group after 4 weeks of modeling. A total of 6298 proteins were identified, among which 348 were differentially expressed proteins (DEPs), with 81 upregulated and 267 downregulated. These DEPs were subjected to in-depth bioinformatics analyses, including Gene Ontology, the Eukaryotic Orthologous Groups, and the Kyoto Encyclopedia of Genes and Genomes. These analyses revealed significant involvement in cellular processes, metabolic pathways, biological regulation, cytoskeletal organization, and cell movement. Our results indicate that calcium signaling plays a critical role in mediating eye changes associated with form deprivation, which may bear similarities to mechanisms observed in neurodegenerative diseases. A total of 348 DEPs related to the development and progression of myopia were identified. These changes involve key biological processes, including protein degradation, cell adhesion, and transport, especially alterations in calcium signaling pathways. Stromal interaction molecule 1 (STIM1) is an important biological marker of FDM, which was confirmed by Western blot, immunohistochemistry and ELISA. Our study found clear differences in the expression of proteins in the vitreous during the development of myopic guinea pigs, especially those related to calcium signaling pathway. Our study offers new insights into the pathogenesis of myopia, particularly changes related to protein metabolism pathways.</p></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"7 ","pages":"Pages 15-24"},"PeriodicalIF":0.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949723X24000126/pdfft?md5=c7b83f5cf95221d34931ea50c1ab4a18&pid=1-s2.0-S2949723X24000126-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterials for cardiovascular diseases","authors":"Yongqi Huang ,&nbsp;Ziqi Ding","doi":"10.1016/j.bmt.2024.05.001","DOIUrl":"https://doi.org/10.1016/j.bmt.2024.05.001","url":null,"abstract":"<div><p>Cardiovascular disease (CVD) remains a leading cause of mortality worldwide, claiming countless lives and posing a formidable health challenge. Extensive efforts have been dedicated to combating this disease, including the development of innovative biomaterials for blood vessel stents, drug delivery and imaging diagnosis. These advancements have substantially addressed issues such as patient rejection and blood contamination. In this review, we begin by outlining the prevalence and various forms of CVD. Subsequently, we delve into advanced biomaterials—including synthetic biomaterials, natural biomaterials, and hybrid biomaterials—and medical instruments used in the treatment or alleviation of CVD, discussing their advantages and limitations. Finally, we offer insights into future prospects and strategies for refining these technologies and instruments moving forward.</p></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"7 ","pages":"Pages 1-14"},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949723X24000114/pdfft?md5=cb5784637ec851af912f15042bb6e167&pid=1-s2.0-S2949723X24000114-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141263997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum regarding missing Declaration of Competing Interest statements in previously published articles","authors":"","doi":"10.1016/j.bmt.2024.04.001","DOIUrl":"https://doi.org/10.1016/j.bmt.2024.04.001","url":null,"abstract":"","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"6 ","pages":"Pages 91-92"},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949723X24000023/pdfft?md5=8230957c0b6cf2e210228a9aa1877084&pid=1-s2.0-S2949723X24000023-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140543654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}