Biomedical Technology最新文献

{"title":"A dual-network hydrogel technology integrating suture-like sealing, photothermal antibacterial activity and immune reprogramming for drug-resistant wound repair","authors":"Linhan Feng,&nbsp;Chunxiao Wang,&nbsp;Ning Wang,&nbsp;Ashokkumar Sibiya,&nbsp;Eliana Pereira de Araujo,&nbsp;Xinyu Zhang,&nbsp;Xianjiao Shao,&nbsp;Panpan Pan,&nbsp;Youbai Chen,&nbsp;Jingdi Chen","doi":"10.1016/j.bmt.2026.100170","DOIUrl":"10.1016/j.bmt.2026.100170","url":null,"abstract":"<div><h3>Background</h3><div>Drug-resistant, infection-associated wounds remain difficult to heal because persistent inflammation, oxidative stress, and biofilm formation disrupt the repair microenvironment, and current dressings often lack both mechanical adaptability and active antibacterial-regenerative functions.</div></div><div><h3>Technology</h3><div>This study introduces a structure–function synergistic engineering strategy to construct a near-infrared (NIR)-responsive multifunctional hydrogel for infected wound repair. Rather than simply integrating multiple functional components, the strategy employs a dual-crosslinked network in which a boronic ester covalent network provides structural stability, while dynamic polydopamine (PDA)–Fe³⁺ coordination simultaneously functions as a flexible molecular bridge, photothermal transducer and immunomodulatory interface. This rational network coupling enables the coordinated integration of mechanical adaptability, antibacterial activity and immune microenvironment regulation within a single hydrogel platform, overcoming the trade-off between structural robustness and biological functionality commonly encountered in multifunctional wound dressings.</div></div><div><h3>Results</h3><div>The engineered hydrogel exhibited excellent mechanical robustness, tissue adhesion, self-healing ability, antioxidant activity and electrical conductivity. Under NIR irradiation, FCPBA-EPL/PVA/PDA-Fe³⁺ dual-crosslinked hydrogel (FPP) inhibited Methicillin-resistant Staphylococcus aureus (MRSA) by 98.06% <em>in vitro</em> and suppressed biofilm formation. In a rat infected full-thickness wound model, FPP + NIR accelerated closure, achieving complete wound closure by day 14, with denser collagen deposition and reduced inflammation. The hydrogel also showed good hemocompatibility and supported fibroblast migration and proliferation.</div></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"15 ","pages":"Article 100170"},"PeriodicalIF":16.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning–guided senescence signatures identify shikonin as an anti-inflammatory therapy for keloid","authors":"Xiaobo Zhou,&nbsp;Jui-Ming Lin,&nbsp;Wenyu Wu,&nbsp;Jun Chen,&nbsp;Jia Huang","doi":"10.1016/j.bmt.2026.100168","DOIUrl":"10.1016/j.bmt.2026.100168","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Keloid is a chronic inflammatory disorder resulting from dysregulated wound healing, characterized by tumor-like aggressiveness and a high recurrence rate. Current treatment modalities remain unsatisfactory. Cellular senescence (CS) and the senescence-associated secretory phenotype (SASP) are persistently present in keloid and may promote disease progression by secreting pro-inflammatory factors and inducing senescence in neighboring cells; however, the underlying regulatory mechanisms remain largely unexplored. Shikonin, a major bioactive component from traditional Chinese medicine, exhibits anti-proliferative, anti-inflammatory, and pro-apoptotic activities, but its therapeutic potential in keloid and its relationship with cellular senescence require further investigation.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Technology&lt;/h3&gt;&lt;div&gt;We profiled CS in keloid using GEO transcriptomes supplemented by RNA sequencing. Differentially expressed genes (DEGs) were identified, and CS-associated DEGs (CS-DEGs) were annotated via the CellAge database, followed by GO/KEGG enrichment analyses. To prioritize clinically relevant CS-DEGs, we applied an AI-driven machine learning (ML) gene-prioritization pipeline to identify hub biomarkers/targets. Immune microenvironment composition was quantified using CIBERSORT algorithmic deconvolution, estimating 22 immune cell subsets from bulk expression data. Subsequently, &lt;em&gt;in vitro&lt;/em&gt; and &lt;em&gt;ex vivo&lt;/em&gt; organ culture experiments were conducted to validate the effects of shikonin on the senescent phenotype, SASP factor expression, and apoptosis in keloid fibroblasts (KFs), supported by &lt;em&gt;in silico&lt;/em&gt; molecular docking to evaluate shikonin–target interactions.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;We identified 187 CS-DEGs enriched in inflammation, stress responses, cell-cycle regulation, tumorigenesis, and tumor suppression. ML highlighted AGR2 (anterior gradient 2) and MDK (midkine) as hub genes with strong diagnostic performance; AGR2 was downregulated and MDK upregulated in keloid and validated in an independent dataset. CIBERSORT revealed increased macrophages M1, which correlated with hub CS-DEGs, supporting a senescence–inflammation axis in keloid. Experimentally, keloid tissues showed increased senescence-associated β-galactosidase–positive cells and elevated MDK, TGF-β1, IL-1β, IL-6, IL-8, and P16 expression versus normal skin. Shikonin inhibited KFs proliferation in a time- and dose-dependent manner; reduced MDK, collagen I, TGF-β1, VEGF, IL-1β, IL-6, IL-8, PPARγ, NOTCH1, and BCL-2; and increased AGR2 and BAX in cultured KFs. &lt;em&gt;In silico&lt;/em&gt; molecular docking predicted favorable binding affinity between shikonin and target proteins. &lt;em&gt;In vitro&lt;/em&gt;, shikonin suppressed KFs outgrowth from tissue masses and promoted apoptosis. Collectively, these findings support a CS-associated, inflammation-coupled mechanism in keloid, nominate AGR2 and MDK as candidate biomarkers/targets, and position shi","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"15 ","pages":"Article 100168"},"PeriodicalIF":16.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hybrid tissue engineering construct enabling sustained BMP2 delivery outperforms a clinically used collagen sponge in large bone defect repair: A preclinical study","authors":"Hirotsugu Maekawa,&nbsp;Seyedsina Moeinzadeh,&nbsp;Elaine Lui,&nbsp;Tomohiro Uno,&nbsp;Hossein Vahid Alizadeh,&nbsp;Jiannan Li,&nbsp;Andrea Sofia Flores Perez,&nbsp;Richard Sammy Muniz,&nbsp;Sungwoo Kim,&nbsp;Ashley M. Potter,&nbsp;Chloe Brekhus,&nbsp;Dan Regan,&nbsp;Drew W. Koch,&nbsp;Masanori Kobayashi,&nbsp;Toshiyuki Kawai,&nbsp;Shuichi Matsuda,&nbsp;Ben Gadomski,&nbsp;Jeremiah T. Easley,&nbsp;Jeffrey L. Young,&nbsp;Michael Gardner,&nbsp;Yunzhi P. Yang","doi":"10.1016/j.bmt.2026.100167","DOIUrl":"10.1016/j.bmt.2026.100167","url":null,"abstract":"<div><h3>Background</h3><div>Large bone defects remain a major challenge in orthopedic surgery. Current treatments, including autologous bone grafting, collagen sponge-based BMP2 delivery, and distraction osteogenesis, are limited by donor-site morbidity, burst BMP2 release causing adverse effects, and surgical invasiveness. Engineered biomaterial platforms combining structural support with sustained growth-factor delivery offer a promising alternative, but few systems integrate structurally defined scaffold design, stable hydrogel incorporation, and compatibility with terminal sterilization workflows.</div></div><div><h3>Technology</h3><div>Here, we evaluated HyTEC, a hybrid tissue engineering platform that integrates a polycaprolactone–β-tricalcium phosphate scaffold with a GelMA/PEGDMA/alginate hydrogel network for sustained BMP2 delivery. A multistep surface-modification strategy combining covalent GelMA conjugation and surface-initiated alginate crosslinking was used to stabilize the scaffold–hydrogel interface. This integrated architecture combines structural support, controlled BMP2 delivery, compatibility with freeze-drying and terminal E-beam sterilization, and can be configured as a solid rod implant or a macroscopic 3D-printed porous scaffold. HyTEC was compared with an equal-dose BMP2-loaded collagen sponge control in a rat critical-sized femoral defect model, and scale-up feasibility was assessed in an exploratory sheep 5-cm metatarsal defect model.</div></div><div><h3>Results</h3><div>In vitro, rod-format HyTEC released 26.5% of BMP2 within 1 day and 79.9% by day 28, whereas the collagen sponge released 67.4% within 24 h. In the 3D-printed porous scaffold format, cumulative BMP2 release reached 17.2 ± 8.1% by day 7 and 62.7 ± 4.0% by day 70 and was monitored over 91 days. hMSC viability in HyTEC remained 92–96% over 7 days, confirming cytocompatibility of the scaffold–hydrogel construct. In the rat model, BMP2-loaded HyTEC achieved 100% bone union by POD 34 and significantly greater BV/TV at POD 55 than the equal-dose collagen sponge control (4.1-fold, P &lt; 0.0001), together with greater mechanical recovery. In the pilot sheep study, the BMP2-loaded HyTEC scaffold specimen had a bone volume of 5126 mm³, compared with 2466 mm³ for autograft and 1942 mm³ for the collagen sponge, without obvious adverse tissue reactions; these observations do not permit statistical inference. These findings support HyTEC as a tissue engineering platform for sustained BMP2 delivery that is geometrically adaptable across implant scales. Powered large-animal studies, dose optimization, mechanistic evaluation, and safety assessment are needed to support clinical translation.</div></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"15 ","pages":"Article 100167"},"PeriodicalIF":16.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Latest advances and frontier applications of engineered cartilage organoids","authors":"Xu Chen,&nbsp;Mibo Lu,&nbsp;Longhui Xu,&nbsp;Guoshuang Zheng,&nbsp;Weiting Yu,&nbsp;Qingzhong Zhang,&nbsp;Zhongyang Liu,&nbsp;Risa Harada,&nbsp;Hui Xie","doi":"10.1016/j.bmt.2026.100169","DOIUrl":"10.1016/j.bmt.2026.100169","url":null,"abstract":"<div><h3>Background</h3><div>Cartilage injuries and osteoarthritis remain major clinical challenges because of the limited intrinsic regenerative capacity of articular cartilage and the inability of conventional two-dimensional cultures and tissue-engineered constructs to fully reproduce its complex cellular, extracellular matrix, and biomechanical microenvironment. Cartilage organoids have therefore emerged as promising three-dimensional models that combine tissue-level self-organization with physiologically relevant cartilage phenotypes.</div></div><div><h3>Technology</h3><div>This review systematically summarizes recent advances in engineered cartilage organoids, with particular emphasis on their construction principles, functional characteristics, biomedical applications, and translational challenges. Major technological determinants are discussed, including cell sources such as mesenchymal stem cells, primary chondrocytes, and induced pluripotent stem cells; extracellular matrix and hydrogel systems; cytokines and differentiation inducers; and engineering strategies involving scaffold-free self-assembly, scaffold-assisted culture, 3D bioprinting, bioreactors, microfluidic platforms, gene editing, and computational approaches.</div></div><div><h3>Results</h3><div>Engineered cartilage organoids have demonstrated substantial potential for disease modeling, drug screening, genetic and microenvironmental regulation, and cartilage or osteochondral regeneration by reproducing pathological phenotypes and enabling more physiologically relevant therapeutic evaluation. However, major barriers remain, including phenotypic instability during long-term culture, incomplete control of cartilage–bone and vascular interactions, insufficient biomechanical integration with host tissue, batch-to-batch variability, and unresolved regulatory and ethical issues. Overall, engineered cartilage organoids provide a versatile bridge between mechanistic cartilage research, therapeutic screening, and regenerative medicine, although further standardization and validation are required before widespread clinical translation.</div></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"15 ","pages":"Article 100169"},"PeriodicalIF":16.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148951135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated strategy of collagen fiber arrangement-inspired magnetic nanochain-doped ordered biphasic scaffold and gradient magnetic field stimulation for osteochondral regeneration","authors":"Junwei Xu ,&nbsp;Yi Cui ,&nbsp;Xuemei Sun ,&nbsp;Zhiheng Chen ,&nbsp;Kun Li ,&nbsp;Meili Liu ,&nbsp;Ping Li","doi":"10.1016/j.bmt.2026.100134","DOIUrl":"10.1016/j.bmt.2026.100134","url":null,"abstract":"<div><h3>Background</h3><div>Osteochondral defects pose significant clinical challenges owing to the complex anisotropic collagen alignment of osteochondral tissue and its limited self-healing capacity. Although mechanically biomimetic scaffolds have been widely applied in osteochondral repair, existing scaffolds exhibit limited structural and functional biomimicry, resulting in osteochondral repair efficacy that requires further improvement.</div></div><div><h3>Technology</h3><div>Bioinspired by the unique collagen fiber alignment of natural osteochondral tissue, this study developed a technology of magnetically guided ordered biphasic scaffold combined with gradient magnetic field stimulation. Via alkaline dissolution and thermal crosslinking, Fe₃O₄ nanochains (NCs) were horizontally oriented in the agarose-based cartilage phase and vertically oriented in the poly(ethylene glycol) diacrylate/agarose-based subchondral bone phase. This scaffold system synergized with a 3–15 mT gradient magnetic field (MF) to enable the integrated repair of osteochondral defects.</div></div><div><h3>Results</h3><div>We adjusted the scaffold's magnetism by modulating the content of Fe₃O₄ NCs, and further investigated the impacts of the magnetic ordered scaffolds and external MF on the differentiation of bone marrow mesenchymal stem cells. Results showed that the cartilage-phase scaffold (0.025 % w/v NCs, 0.27 emu/g) upregulated type II collagen (chondrogenesis), while the subchondral bone-phase scaffold (1.0 % w/v NCs, 1.20 emu/g) boosted osteogenic differentiation. Specifically, 3 mT static MF enhanced chondrogenesis via ECM-receptor signaling, while 15 mT static MF stimulated osteogenesis by activating PI3K/Akt pathway. Animal studies demonstrated that the magnetic biphasic hierarchical scaffold combined with 3–15 mT gradient MF significantly improved osteochondral repair, including nearly double the new subchondral bone volume fraction, a smoother cartilage surface, and collagen fiber alignment that more closely resembled natural osteochondral tissue. This work highlights the potential of the magnetic ordered scaffold-gradient MF technology in osteochondral repair, and is further poised for synergistic development with 3D bioprinting, intelligent manufacturing, and single-cell sequencing, injecting new impetus into the clinical translation of magnetic tissue engineering.</div></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"13 ","pages":"Article 100134"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Barcode screening reveals the key role of gold nanoparticle morphology in tumor targeting","authors":"Chaojin Li ,&nbsp;Feng Gong ,&nbsp;Xiaodong Liu ,&nbsp;Ang Li","doi":"10.1016/j.bmt.2026.100135","DOIUrl":"10.1016/j.bmt.2026.100135","url":null,"abstract":"<div><div>Currently, nanoparticles (NPs) still face challenges in achieving efficient tumor targeted delivery, especially in balancing in vivo distribution and cellular uptake efficiency. A recent study published in Advanced Functional Materials utilized DNA barcoding combined with high-throughput in vivo screening to systematically evaluate the targeting of gold NPs of different shapes and sizes in tumors. The results showed that large-sized triangular gold NPs exhibited the highest cellular uptake efficiency in vitro, and achieved significant tumor enrichment and excellent siRNA delivery in vivo through surface modification of RGD peptides. Further photothermal experiments showed that triangular particles can rapidly raise the temperature to 57 °C in the tumor area, achieving effective tumor ablation. This study indicates that large triangular gold NPs possess advantages in cellular uptake, in vivo enrichment ability, and photothermal therapy potential, providing a new strategy for precise tumor targeted therapy. The study highlights that nanoparticle morphology plays a dominant role in governing in vivo tumor accumulation and therapeutic outcomes.</div></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"13 ","pages":"Article 100135"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated tardigrade-based biomimetic strategy and stem cell expansion and differentiation for a novel radioprotection approach","authors":"Biao Zhang ,&nbsp;Jun-Nian Zhou ,&nbsp;Quan Zeng ,&nbsp;Zhi-Rui Liu,&nbsp;Yu-Ting Gao,&nbsp;Fu-Dong Chen,&nbsp;Tao Fan,&nbsp;Ya-Li Jia,&nbsp;Jia-Fei Xi,&nbsp;Xue-Tao Pei,&nbsp;Wen Yue","doi":"10.1016/j.bmt.2025.100132","DOIUrl":"10.1016/j.bmt.2025.100132","url":null,"abstract":"<div><h3>Background</h3><div>Tardigrades have attracted widespread research attention due to their extraordinary radiotolerance, of which the damage suppressor protein (DSUP) is regarded as a key molecule responsible for their robust DNA repair capability. How to translate these findings into safe and efficacious radioprotection strategies for humans remains an ongoing research challenge.</div></div><div><h3>Technology</h3><div>Given that ionizing radiation (IR)-induced hematopoietic failure caused by depletion of bone marrow hematopoietic stem/progenitor cells (HSPCs) is a major cause of mortality, we propose using gene editing and stem cell technologies to precisely knock in DSUP gene into isolated murine HSPCs, thereby generating HSPCs with enhanced radiotolerance. In this study, we first obtained DSUP-modified mouse HSPCs by isolating, culturing, and transfecting mouse HSPCs using lentivirus transfection, as well as DSUP-modified human HSPCs via three stages of hematopoietic induction and differentiation from DSUP-modified human pluripotent stem cells (PSCs).</div></div><div><h3>Results</h3><div>The radioprotection capacity of <em>DSUP</em>-modified stem cells was confirmed by a series of <em>in vitro</em> assays. Given the differentiation potential of myeloid progenitor cells (MPCs) and without requirement for human leukocyte antigen matching during hematopoietic stem cell transplant, we then obtained <em>DSUP</em>-modified mouse MPCs by differentiated from the expanded mouse HSPCs in polyvinyl alcohol (PVA) culture system for more than 30 days. <em>DSUP</em>-modified MPCs can also maintain lower radiation-induced apoptosis and DNA damage. Pre-infusion of <em>DSUP</em>-modified MPCs improves irradiated-mice survival rate by 30 % without long-term side effects. While not residing in bone marrow or spleen, these cells alleviated hematopoietic failure by restoring peripheral red blood cells and platelets and accelerated hematopoietic recovery. Mechanistically, DSUP forms phase separation structures that can recruit DNA repair proteins to double-strand breaks, promoting homologous recombination repair. Taken together, our results demonstrated <em>DSUP</em>-modified MPCs offer a promising stem cell-based radioprotection technology, highlighting a novel biomimetic approach for radioprotection.</div></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"13 ","pages":"Article 100132"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible screw-based biomechanical alignment technique for improving surgical effect in adolescent flexible flatfoot","authors":"Xiaofan Wang ,&nbsp;Qing Lin ,&nbsp;Lifeng Qiu ,&nbsp;Yang Liu ,&nbsp;Siyan Guan ,&nbsp;Zhizhi Luo ,&nbsp;Yang Wang ,&nbsp;Shuncai Hu ,&nbsp;Feng Lin","doi":"10.1016/j.bmt.2026.100136","DOIUrl":"10.1016/j.bmt.2026.100136","url":null,"abstract":"<div><h3>Background</h3><div>The surgical management of adolescent flexible flatfoot with accessory navicular injury remains controversial.</div></div><div><h3>Technology</h3><div>We developed a minimally invasive technique combining biocompatible screw–based hindfoot biomechanical alignment correction with Kidner surgery. A biocompatible screw was implanted in the tarsal sinus to stabilize the subtalar joint and maintain normal anatomical relationships of the talocalcaneal and talonavicular joints, thereby correcting hindfoot alignment.</div></div><div><h3>Results</h3><div>Postoperatively, the hindfoot valgus angle improved significantly from 11.3° ± 3.3° to 3.0° ± 1.6° (<em>P</em> &lt; 0.001), and the Meary angle decreased from 19.8° ± 9.4° to 4.6° ± 4.1° (<em>P</em> &lt; 0.001), indicating effective reconstruction of hindfoot and midfoot alignment. Functional outcomes also improved markedly: AOFAS scores rose from 71.6 ± 12.8 preoperatively to 93.7 ± 8.1 postoperatively (<em>P</em> &lt; 0.001), and VAS pain scores dropped from 6.1 ± 3.1 to 2.6 ± 1.1 (<em>P</em> &lt; 0.001). No serious complications were observed during follow-up, and the biocompatible screws demonstrated excellent stability and biocompatibility.</div></div><div><h3>Conclusion</h3><div>Our findings suggest that this combined approach effectively corrects pathological biomechanics, achieves anatomical realignment, and significantly enhances functional recovery while alleviating pain. It represents a safe, feasible, and promising strategy for treating adolescent flexible flatfoot with accessory navicular injury, offering a novel pathway toward precise, minimally invasive care.</div></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"13 ","pages":"Article 100136"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decellularized extracellular matrix: Advanced bioplatforms for functional tissue restoration via innovative decellularization techniques","authors":"Zhe Wang ,&nbsp;Xiang Lin ,&nbsp;Yunpeng Shi ,&nbsp;Hong Yan ,&nbsp;Yixuan Shang ,&nbsp;Haozhen Ren","doi":"10.1016/j.bmt.2025.100131","DOIUrl":"10.1016/j.bmt.2025.100131","url":null,"abstract":"<div><h3>Background</h3><div>In recent years, tissue engineering has experienced rapid development, with bioscaffolds emerging as a focal point of research due to their favorable bioactivity, biocompatibility, and capacity to provide mechanical support for cellular growth. The bioscaffolds have great potential in tissue regeneration. However, conventional natural scaffolds and polymer scaffolds pose risks of immunogenicity, while also face challenges in mimicking the <em>in vivo</em> microenvironment and the biochemical and mechanical properties of natural organs/tissues, which collectively limit their repair capability. The development of decellularized extracellular matrix (dECM) technology offers a viable solution to these challenges, demonstrating considerable potential for advancing organ and tissue regeneration.</div></div><div><h3>Technology</h3><div>This reviews the classification of dECM, outlines various current methods for its preparation, and comprehensively examines its latest advances in tissue repair and regenerative medicine, including applications in skin, bone, nerve, heart, lung, liver, and kidney tissues.</div></div><div><h3>Results</h3><div>This review systematically examines recent advances in dECM production and regenerative medicine applications. We classify dECM subtypes, detail contemporary decellularization protocols, and highlight their biomedical utility. Superior biocompatibility substantially mitigates post-transplant immune rejection risk, underscoring strong clinical translation potential for tissue engineering.</div></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"13 ","pages":"Article 100131"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145915116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress of computational biomechanical modelling for calcaneal fracture fixation","authors":"Duo Wai-Chi Wong ,&nbsp;James Chung-Wai Cheung ,&nbsp;Lucci Lugee Liyeung ,&nbsp;Esther Man-Wai Chow ,&nbsp;Winson Chiu-Chun Lee ,&nbsp;Wenxin Niu ,&nbsp;Ming Ni","doi":"10.1016/j.bmt.2026.100133","DOIUrl":"10.1016/j.bmt.2026.100133","url":null,"abstract":"<div><h3>Background</h3><div>Calcaneal fractures are among the most disabling and costly orthopaedic injuries, frequently requiring surgical fixation via open reduction and internal fixation (ORIF) or minimally invasive fixation (MIF). Finite element (FE) analysis is an increasingly critical <em>in silico</em> tool for optimizing implant designs and comparing fixation constructs, yet the technical modelling strategies and limitations specific to calcaneal fracture biomechanics have not been systematically mapped.</div></div><div><h3>Technology</h3><div>The FE modelling pipeline comprises sequential technical domains: (1) medical image-based geometry reconstruction and mesh generation; (2) material assignment (elastic modulus, Poisson's ratio, constitutive laws for bone, cartilage, and ligaments); (3) virtual fracture creation (gap width specification, Sanders classification implementation); (4) implant insertion (plate/screw/nail geometry, contact definition); (5) boundary conditions (standing/gait loading protocols, constraint schemes, muscle force incorporation); (6) solver configuration (static/quasi-static analysis); and (7) outcome extraction (von Mises stress, displacement, construct stiffness, micromotion metrics, etc.). This systematic scoping review followed JBI methodology and PRISMA-ScR guidelines to identify FE studies from PubMed, Web of Science, Scopus, and IEEE Xplore. Methodological quality was evaluated using the MQSSFE instrument for computational orthopaedic models.</div></div><div><h3>Results</h3><div>Twenty-three studies were included, predominantly using single-subject CT models with artificially created Sanders type II–III intra-articular fractures. Most employed calcaneus-only geometries, linear elastic isotropic bone properties, tetrahedral meshes, and quasi-static stance loading. Locking plates, hybrid plate–screw constructs, screw-only MIF, and intramedullary nails were compared via stress distribution and construct stiffness. Several studies introduced topology-optimized plates and micromotion-based fracture gap metrics, demonstrating that MIF with supplementary percutaneous screws can achieve biomechanical stability comparable to ORIF. However, verification (mesh convergence) and validation procedures were inconsistently reported, dynamic loading and multi-patient cohorts were rare, and interfragmentary strain–based healing criteria were largely absent. This delineation of the current technical design space highlights priorities for more physiologically realistic and methodologically robust <em>in silico</em> studies.</div></div>","PeriodicalId":100180,"journal":{"name":"Biomedical Technology","volume":"13 ","pages":"Article 100133"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}