Bioimaging最新文献_第4页

Optimizing the strength of an evanescent wave generated from a prism coated with a double-layer thin-film stack 优化由双层薄膜堆覆盖的棱镜产生的倏逝波的强度

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199703)5:1<1::AID-BIO1>3.0.CO;2-0

Pu Chun Ke, X S Gan, Jakub Szajman, S Schilders, M Gu

引用次数: 0

Three-dimensional microscopy in thick biological samples: A fresh approach for adjusting focus and correcting spherical aberration 厚生物样品中的三维显微镜:一种调整焦点和校正球差的新方法

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199703)5:1<40::AID-BIO4>3.0.CO;2-W

Z Kam, D A Agard, J W Sedat

引用次数: 0

Quantitative evaluation of light microscopes based on image processing techniques 基于图像处理技术的光学显微镜定量评价

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199809)6:3<138::AID-BIO4>3.0.CO;2-8

L R van den Doel, A D Klein, S L Ellenberger, H Netten, F R Boddeke, L J van Vliet, I T Young

{"title":"Quantitative evaluation of light microscopes based on image processing techniques","authors":"L R van den Doel, A D Klein, S L Ellenberger, H Netten, F R Boddeke, L J van Vliet, I T Young","doi":"10.1002/1361-6374(199809)6:3<138::AID-BIO4>3.0.CO;2-8","DOIUrl":"10.1002/1361-6374(199809)6:3<138::AID-BIO4>3.0.CO;2-8","url":null,"abstract":"In this note we will present methods based on image processing techniques to evaluate the performance of light microscopes. These procedures are applied to three different ‘high-end’ light microscopes. Tests are carried out to measure the homogeneity of the illumination system. From these tests it follows that Köhler illuminated images can have an exceedingly high amount of shading. Another result found from the illumination calibration is that traditional lamp housings are not designed to make fine-tuning easy. Next, the (automated) stage is considered. Several tests are performed to measure the stage motion in the xy-plane and in the axial direction to address accuracy, precision, and hysteresis effects. Finally, the entire electro-optical system is characterized by measuring the optical transfer function (OTF) at wavelengths 400 nm, 500 nm, 600 nm, and 700 nm. The results of these experiments show that there is a consistent deviation from the theoretical OTF at wavelengths around 400 nm. The final conclusion is that modern light microscopes perform better than their five-to-ten-year-old predecessors.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"6 3","pages":"138-149"},"PeriodicalIF":0.0,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199809)6:3<138::AID-BIO4>3.0.CO;2-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72573435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Number analysis of fluorescence correlation spectroscopy for the cleaving process of fluorescence labeled DNA 荧光标记DNA切割过程的荧光相关光谱数值分析

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199709)5:3<129::AID-BIO6>3.0.CO;2-6

Goro Nishimura, Rudolf Rigler, Masataka Kinjo

引用次数: 12

Near-field optical microscopy for DNA studies at the single molecular level 用于单分子水平DNA研究的近场光学显微镜

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199803)6:1<43::AID-BIO6>3.0.CO;2-F

M F Garcia-Parajo, J-A Veerman, S J T van Noort, B G de Grooth, J Greve, N F van Hulst

{"title":"Near-field optical microscopy for DNA studies at the single molecular level","authors":"M F Garcia-Parajo, J-A Veerman, S J T van Noort, B G de Grooth, J Greve, N F van Hulst","doi":"10.1002/1361-6374(199803)6:1<43::AID-BIO6>3.0.CO;2-F","DOIUrl":"10.1002/1361-6374(199803)6:1<43::AID-BIO6>3.0.CO;2-F","url":null,"abstract":"An aperture-type near-field optical microscope (NSOM) with two polarization detection channels has been used to image fluorescently labelled DNA with high spatial resolution and single molecule fluorescence sensitivity. The sample has been engineered such that there is only one rhodamine dye per DNA strand. Lateral and vertical DNA dimensions in the shear-force image are 14 +/- 2 nm and 1.4 +/- 0.2 nm, respectively. No sample deformation was observed under our imaging conditions. Near-field fluorescence imaging of individual fluorophores shows an optical resolution of 70 nm at full-width at half- maximum. Large intensity differences between individual rhodamine molecules attached to DNA are observed from the NSOM images. Statistics on rhodamine dyes in different environments (attached to glass, embedded in a polymer layer and attached to DNA) show bleaching rates of 10(-5). Total intensity line profiles together with in-plane angle orientation are used to characterize individual dyes. Rhodamine dyes show strong intensity fluctuations independent of the particular environment. These results are in contrast with the more stable photophysical behaviour as observed for carbocyanine molecules embedded in polymer matrices. The mobility of rhodamine-both lateral and rotational-is clearly influenced by its immediate surrounding and attachment to the surface.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"6 1","pages":"43-53"},"PeriodicalIF":0.0,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199803)6:1<43::AID-BIO6>3.0.CO;2-F","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79975553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Multifocal multiphoton microscopy: A fast and efficient tool for 3-D fluorescence imaging 多焦点多光子显微镜:一种快速有效的三维荧光成像工具

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199812)6:4<177::AID-BIO3>3.0.CO;2-R

Martin Straub, Stefan W Hell

引用次数: 63

Strategy for room temperature spectroscopy of single molecules 单分子室温光谱分析策略

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199709)5:3<99::AID-BIO3>3.0.CO;2-W

T Ha, D S Chemla, Th Enderle, S Weiss

引用次数: 0

Fast algorithms for the analysis of single and double exponential decay curves with a background term. Application to time-resolved imaging microscopy 具有背景项的单指数和双指数衰减曲线的快速分析算法。应用于时间分辨成像显微镜

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199703)5:1<19::AID-BIO3>3.0.CO;2-B

Theodorus W J Gadella Jr, Thomas M Jovin

{"title":"Fast algorithms for the analysis of single and double exponential decay curves with a background term. Application to time-resolved imaging microscopy","authors":"Theodorus W J Gadella Jr, Thomas M Jovin","doi":"10.1002/1361-6374(199703)5:1<19::AID-BIO3>3.0.CO;2-B","DOIUrl":"https://doi.org/10.1002/1361-6374(199703)5:1<19::AID-BIO3>3.0.CO;2-B","url":null,"abstract":"Computer programs have been developed to determine decay time constants from a temporal sequence of digitized images with decaying intensities characterized by either single or double exponentials plus a constant background term. The very fast algorithms are evaluated at every pixel position. A non-iterative Prony-like method provides high quality initial estimates that are used for the subsequent non-linear least-squares procedure in which the normal equations are solved directly. Error analysis routines enable a pixel-by-pixel estimation of the quality of the experimental data. The stand-alone programs were fully integrated into a commercial image-processing environment (SCIL-Image) for a convenient and optimal display of the decay analysis. The features of the programs are illustrated by the analysis of simulated image data. With current workstations, the fitting routines (including reading of data, initial estimate and error analysis) require 0.13 ms/pixel using the single exponential algorithm applied to 50 time points, and 1.37 ms/pixel for 100 time points and the double exponential algorithm. The programs are of general applicability and have been used to analyse data from time-resolved fluorescence, phosphorescence, and photobleaching-based microscopy. Two examples of the latter case are shown, illustrating the utility of the programs for the quantitative evaluation of spatially resolved fluorescence resonance energy transfer (FRET) and for generating contrast by allocating specific cellular structures to particular decay components in a fluorescence image.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"5 1","pages":"19-39"},"PeriodicalIF":0.0,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199703)5:1<19::AID-BIO3>3.0.CO;2-B","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137670539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust cell nuclei segmentation using statistical modelling 稳健细胞核分割使用统计建模

Bioimaging Pub Date : 1998-06-01 DOI: 10.1002/1361-6374(199806)6:2<79::AID-BIO3>3.0.CO;2-#

T. Mouroutis, S. Roberts, A. Bharath

引用次数: 106

Efficient suppression of diffusing photons using polarising annular objectives for microscopic imaging through turbid media 利用偏振环形物镜对混浊介质显微成像的有效抑制扩散光子

Bioimaging Pub Date : 1998-06-01 DOI: 10.1002/1361-6374(199806)6:2<92::AID-BIO4>3.0.CO;2-F

S. Schilders, X. Gan, M. Gu

引用次数: 4