Bioimaging最新文献

Magnetic Resonance Imaging 磁共振成像

Bioimaging Pub Date : 2020-05-26 DOI: 10.1201/9780429260971-4

S. Ueno

引用次数: 0

Magnetic Particle Imaging 磁粉成像

Bioimaging Pub Date : 2020-05-26 DOI: 10.1201/9780429260971-7

K. Enpuku, Takashi Yoshida

引用次数: 0

DNA attachment to optically trapped beads in microstructures monitored by bead displacement DNA附着在光学捕获的微结构珠上，由珠位移监测

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199803)6:1<25::AID-BIO4>3.0.CO;2-H

J Dapprich, N Nicklaus

引用次数: 16

Resolution improvement by 3-D reconstructions from tilted views in axial tomography and confocal theta microscopy 通过轴向断层扫描和共聚焦theta显微镜中倾斜视图的三维重建提高分辨率

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199712)5:4<171::AID-BIO1>3.0.CO;2-K

Kurt Sätzler, Roland Eils

{"title":"Resolution improvement by 3-D reconstructions from tilted views in axial tomography and confocal theta microscopy","authors":"Kurt Sätzler, Roland Eils","doi":"10.1002/1361-6374(199712)5:4<171::AID-BIO1>3.0.CO;2-K","DOIUrl":"10.1002/1361-6374(199712)5:4<171::AID-BIO1>3.0.CO;2-K","url":null,"abstract":"The resolution along the optical axis is much less than the lateral resolution in both confocal and conventional fluorescence light microscopy. To correct for the anisotropy in resolution one may generate tilted views from the same object and subsequently merge these tilted views into one image with improved isotropic resolution. It has been suggested to obtain tilted views in confocal theta microscopy with double observation by illuminating the object from one side and simultaneously detecting the emitted light in the direction parallel to the illumination direction and in a direction with an angle theta to the illumination direction. Alternatively, one might use the usual optical set-up of a light microscope together with a tilting device to rotate the object under the microscope. With this method the object can be viewed from all directions (axial tomography). In this paper we investigate two methods for merging tilted views. If the image sets obtained from the different tilt angles are interpreted as statistic samples of one and the same object, a gain in resolution along the optical axis is accompanied by a significant trade-off in lateral resolution. An optical interpretation of the image formation in each single object point by retaining the frequencies with the highest amplitudes in the different tilted views provides a gain in axial resolution without significant decrease in lateral resolution. We will describe a quantitative method to determine the gain in resolution dependent on the number and geometry of the tilted views. It will be demonstrated that a previously suggested reconstruction method implying a multiplication of tilted views in the spatial domain does not improve resolution. Simulated reconstructions based on measured point spread functions in axial tomography and confocal theta microscopy with double observation will demonstrate the validity of our reconstruction methods.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199712)5:4<171::AID-BIO1>3.0.CO;2-K","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78650229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Measuring 3-D plant growth using optical flow 利用光流测量三维植物生长

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199706)5:2<82::AID-BIO5>3.0.CO;2-F

J L Barron, A Liptay

引用次数: 0

Fluorescence correlation spectroscopy as a detection tool of point mutation in genes 荧光相关光谱作为基因点突变的检测工具

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199709)5:3<134::AID-BIO7>3.0.CO;2-H

Masataka Kinjo, Goro Nishimura

引用次数: 0

Effects of Fresnel diffraction on confocal imaging with an annular lens 菲涅耳衍射对环形透镜共聚焦成像的影响

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199709)5:3<153::AID-BIO9>3.0.CO;2-C

X Gan, C J R Sheppard, M Gu

引用次数: 0

Multiparameter image cytometry: From confocal micrographs to subcellular fluorograms 多参数图像细胞术:从共聚焦显微图到亚细胞荧光图

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199709)5:3<159::AID-BIO10>3.0.CO;2-5

Denis Demandolx, Jean Davoust

{"title":"Multiparameter image cytometry: From confocal micrographs to subcellular fluorograms","authors":"Denis Demandolx, Jean Davoust","doi":"10.1002/1361-6374(199709)5:3<159::AID-BIO10>3.0.CO;2-5","DOIUrl":"https://doi.org/10.1002/1361-6374(199709)5:3<159::AID-BIO10>3.0.CO;2-5","url":null,"abstract":"Multifluorescence labeling is routinely performed to detect the spatial coincidence between several markers within biological specimens. We have recently developed image correlation methods to identify double fluorescent structures by virtue of local similarities between fluorescence distributions. We extend this approach here to analyze statistically the fluorescence distribution of structures of interest on micrographs. This digital cytometry relies mainly on the segmentation of multifluorescence images. Once identified, all objects are analyzed through a range of attributes estimating size, morphology, fluorescence content and mean colocalization level between fluorescence channels. The data sets which are saved in flow cytometry standard (FCS) files, allow multiparameter classification of objects and subpopulation counting. The combination of fluorescence, morphometric and local image correlation attributes has been applied here to compare the frequency of single- and multiple-labeled structures at the subcellular level.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199709)5:3<159::AID-BIO10>3.0.CO;2-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137670536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Fourier–Shannon approach to closed contours modelling 闭合轮廓建模的傅里叶-香农方法

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199809)6:3<111::AID-BIO1>3.0.CO;2-3

Claudia Bonciu, Christophe Léger, Jacques Thiel

{"title":"A Fourier–Shannon approach to closed contours modelling","authors":"Claudia Bonciu, Christophe Léger, Jacques Thiel","doi":"10.1002/1361-6374(199809)6:3<111::AID-BIO1>3.0.CO;2-3","DOIUrl":"10.1002/1361-6374(199809)6:3<111::AID-BIO1>3.0.CO;2-3","url":null,"abstract":"This paper describes a modelling method for continuous closed contours. The initial input data set consists of two-dimensional (2-D) points, which may be represented as a discrete function in a polar coordinate system. The method uses the Shannon interpolation between these data points to obtain the global continuous contour model. A minimal description of the contour is obtained using the link between the Shannon interpolation kernel and the Fourier series of polar development (FSPD) for periodic functions. The Shannon interpolation kernel allows the direct interpretation of the contour smoothness in terms of both samples and Fourier frequency domains.In order to deal with deformation point sources, often encountered in active modelling techniques, a method of local deformation is proposed. Each local deformation is performed in an angular sector centred on the deformation point source. All the neighbouring characteristic samples are displaced in order to minimize the oscillations of the newly created model outside the deformation sector. This deformation technique preserves the frequency characteristics of the contour, regardless of the number and the intensity of deformation sources. In this way, the technique induces a frequency modelling constraint, which may be subsequently used in an active detection and modelling environment.Experiments on synthetic and real data prove the efficiency of the proposed technique. The method is currently used to model contours of the left ventricle of the heart obtained from ultrasound apical images. This work is part of a larger project, the aim of which is to analyse the space and time deformations of the left ventricle. The 2-D Fourier–Shannon model is used as a basis for more complex three-dimensional and four-dimensional Fourier models, able to recover automatically the movement and deformation of the left ventricle of the heart during a cardiac cycle.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199809)6:3<111::AID-BIO1>3.0.CO;2-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85924209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Steps towards spatially resolved single molecule detection in solution 在溶液中实现空间分辨单分子检测的步骤

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199709)5:3<116::AID-BIO5>3.0.CO;2-J

H P Mathis, G Kalusche, B Wagner, J S McCaskill

{"title":"Steps towards spatially resolved single molecule detection in solution","authors":"H P Mathis, G Kalusche, B Wagner, J S McCaskill","doi":"10.1002/1361-6374(199709)5:3<116::AID-BIO5>3.0.CO;2-J","DOIUrl":"https://doi.org/10.1002/1361-6374(199709)5:3<116::AID-BIO5>3.0.CO;2-J","url":null,"abstract":"Spatially resolved single molecule detection in solution is a prerequisite for molecule tracking and provides new opportunities in single molecule manipulation, for example in a sample flow. A detector with fast timing properties and high spatial resolution is required for these purposes. We introduce a concept for spatially resolved optical single molecule detection in an epi-illuminated microscope using a novel kind of detector and a new algorithm in configurable hardware for intelligent data processing. The analysis is performed on a parallel hardware interface and includes burst detection. It can be extended to on-line spatial and temporal data analysis. This detector will be used in combination with a molecular sorter where molecules are sorted in a microstructured flow device made of silicon. In a first step towards such sorting in these microstructures, we show that a reliable detection of single molecules in silicon microstructures is possible in zero-dimensional detection volumes. The noise structure of the data is analysed in terms of Poissonian statistics and it is shown that in the smallest structure used (depth 20 μm) a signal-to-noise ratio of 40 is achieved.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199709)5:3<116::AID-BIO5>3.0.CO;2-J","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137670532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0