Fluorescence correlation spectroscopy as a detection tool of point mutation in genes

Masataka Kinjo, Goro Nishimura
{"title":"Fluorescence correlation spectroscopy as a detection tool of point mutation in genes","authors":"Masataka Kinjo,&nbsp;Goro Nishimura","doi":"10.1002/1361-6374(199709)5:3<134::AID-BIO7>3.0.CO;2-H","DOIUrl":null,"url":null,"abstract":"<p>Fluorescence correlation spectroscopy (FCS) provides information about two important parameters in molecular biology. These are the average number of molecules in the detection volume, and the translational diffusion coefficient of the molecules. Although the properties of the translational diffusion reflect the molecular weight and shape, our work focuses on the analysis of the number of cleaved DNA fragments in solution. The cleavage of fluorescently labelled M13 DNA and pUC19 DNA was carried out with three kinds of restriction enzymes (HaeIII, HgaI and BsmAI), and the number of DNA fragments was monitored using FCS. The reciprocal value of the autocorrelation function at time τ = 0 is consistent with the number of fragments that are expected from restriction enzyme maps of the DNA. Our study indicates the capability of FCS as a tool for finding genetic differences in DNA sequences.</p>","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199709)5:3<134::AID-BIO7>3.0.CO;2-H","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioimaging","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199709%295%3A3%3C134%3A%3AAID-BIO7%3E3.0.CO%3B2-H","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Fluorescence correlation spectroscopy (FCS) provides information about two important parameters in molecular biology. These are the average number of molecules in the detection volume, and the translational diffusion coefficient of the molecules. Although the properties of the translational diffusion reflect the molecular weight and shape, our work focuses on the analysis of the number of cleaved DNA fragments in solution. The cleavage of fluorescently labelled M13 DNA and pUC19 DNA was carried out with three kinds of restriction enzymes (HaeIII, HgaI and BsmAI), and the number of DNA fragments was monitored using FCS. The reciprocal value of the autocorrelation function at time τ = 0 is consistent with the number of fragments that are expected from restriction enzyme maps of the DNA. Our study indicates the capability of FCS as a tool for finding genetic differences in DNA sequences.

荧光相关光谱作为基因点突变的检测工具
荧光相关光谱(FCS)提供了分子生物学中两个重要参数的信息。这些是检测体积中分子的平均数量,以及分子的平动扩散系数。虽然平移扩散的性质反映了分子量和形状,但我们的工作重点是分析溶液中被切割的DNA片段的数量。用三种限制性内切酶(HaeIII、HgaI和BsmAI)对荧光标记的M13 DNA和pUC19 DNA进行切割,并用FCS监测DNA片段的数量。自相关函数在τ = 0时刻的倒数值与DNA限制性内切酶图谱中预期的片段数一致。我们的研究表明FCS可以作为发现DNA序列遗传差异的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信