Bioimaging最新文献_第2页

Time-resolved identification of individual mononucleotide molecules in aqueous solution with pulsed semiconductor lasers 脉冲半导体激光在水溶液中单个单核苷酸分子的时间分辨鉴定

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199803)6:1<14::AID-BIO3>3.0.CO;2-O

Markus Sauer, Jutta Arden-Jacob, Karl H Drexhage, Florian Göbel, Ulrike Lieberwirth, Klaus Mühlegger, Ralph Müller, Jürgen Wolfrum, Christoph Zander

{"title":"Time-resolved identification of individual mononucleotide molecules in aqueous solution with pulsed semiconductor lasers","authors":"Markus Sauer, Jutta Arden-Jacob, Karl H Drexhage, Florian Göbel, Ulrike Lieberwirth, Klaus Mühlegger, Ralph Müller, Jürgen Wolfrum, Christoph Zander","doi":"10.1002/1361-6374(199803)6:1<14::AID-BIO3>3.0.CO;2-O","DOIUrl":"10.1002/1361-6374(199803)6:1<14::AID-BIO3>3.0.CO;2-O","url":null,"abstract":"We applied a short-pulse diode laser emitting at 640 nm with a repetition rate of 56 MHz in combination with a confocal microscope to study bursts of fluorescence photons from individual differently labeled mononucleotide molecules in water. Two newly synthesized dyes, an oxazine dye (MR121) and a rhodamine dye (JA53), and two commercially available dyes, a carbocyanine dye (Cy5) and a bora-diaza-indacene dye (Bodipy630/650), were used as fluorescent labels. The time-resolved fluorescence signals of individual mononucleotiode molecules in water were analyzed and identified by a maximum likelihood estimator (MLE). Taking only those single molecule transits which contain more than 30 collected photoelectrons, the two labeled mononucleotide molecules, Cy5-dCTP and Bodipy-dUTP, can be identified by time-resolved fluorescence spectroscopy with a probability of correct classification of greater than 99%. Our results show that at least three differently labeled mononucleotide molecules can be identified in a common aqueous solution. We obtain an overall classification probability of 90% for the time-resolved identification of Cy5-dCTP, MR121-dUTP and Bodipy-dUTP molecules via their characteristic fluorescence lifetimes of 1.05 ± 0.33 ns (Cy5-dCTP), 2.07 ± 0.59 ns (MR121-dUTP) and 3.88 ± 1.71 ns (Bodipy-dUTP).","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"6 1","pages":"14-24"},"PeriodicalIF":0.0,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199803)6:1<14::AID-BIO3>3.0.CO;2-O","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81059938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Improvement in imaging in confocal fluorescent microscopes using multiple detectors 多探测器共聚焦荧光显微镜成像技术的改进

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199809)6:3<126::AID-BIO2>3.0.CO;2-L

R Gauderon, C J R Sheppard

引用次数: 10

Determination of pore networks and water content distributions from 3-D computed tomography images of a clay soil 黏土三维计算机断层成像中孔隙网络和含水量分布的测定

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199712)5:4<194::AID-BIO3>3.0.CO;2-3

Anton W J Heijs, Jacqueline de Lange

{"title":"Determination of pore networks and water content distributions from 3-D computed tomography images of a clay soil","authors":"Anton W J Heijs, Jacqueline de Lange","doi":"10.1002/1361-6374(199712)5:4<194::AID-BIO3>3.0.CO;2-3","DOIUrl":"10.1002/1361-6374(199712)5:4<194::AID-BIO3>3.0.CO;2-3","url":null,"abstract":"Knowledge of flow in porous media can be improved by analyzing three-dimensional (3-D) images of the water distribution in a soil. We report on such a study using computed tomography (CT) scanning of a clay soil sample first in dry conditions. Then, after an infiltration experiment, by scanning the sample again in wet conditions. We used test phantoms to determine the optimal scanning parameters to obtain images with a bimodal gray value distribution with high contrast and small standard deviation. We also determined the effect of slice thickness and reconstruction algorithm on the restoration and segmentation of the images. For a good 3-D representation of the pore space we scanned the slices adjacently at an axial resolution of 1 mm and a pixel size of 0.27 × 0.27 mm2. The high contrast between air in the pores and the clay background allowed a global thresholding for the segmentation whereby the connectivity and topology of the pore networks is conserved. From the difference between the images of the dry sample and the wet sample we determined the water content distribution. We used the image data for measurements of the water distribution to study the relation between the structure of the pore networks and water flow.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"5 4","pages":"194-204"},"PeriodicalIF":0.0,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199712)5:4<194::AID-BIO3>3.0.CO;2-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84487345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Efficient suppression of diffusing photons using polarising annular objectives for microscopic imaging through turbid media. Bioimaging 6:92–97 1998 利用偏振环形物镜对混浊介质显微成像的有效抑制扩散光子。生物成像，6:92-97 1998

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199809)6:3<150::AID-BIO5>3.0.CO;2-R

S P Schilders, X S Gan, M Gu

引用次数: 1

The effect of spatial filtering on the performance of time-of-flight imaging systems 空间滤波对飞行时间成像系统性能的影响

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199706)5:2<71::AID-BIO4>3.0.CO;2-M

M C Pitter, J G Walker

引用次数: 0

Comparison between time-correlated single photon counting and fluorescence correlation spectroscopy in single molecule identification 时间相关单光子计数与荧光相关光谱在单分子鉴定中的比较

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199803)6:1<3::AID-BIO2>3.0.CO;2-M

Jörg Enderlein, Malte Köllner

引用次数: 11

Confocal microscopy of single molecules of the green fluorescent protein 绿色荧光蛋白的单分子共聚焦显微镜

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199803)6:1<54::AID-BIO7>3.0.CO;2-8

G Jung, J Wiehler, W Göhde, J Tittel, Th Basché, B Steipe, C Bräuchle

引用次数: 43

Heavy metal contrast enhancement for the selective detection of gold particles in electron microscopical sections using electron spectroscopic imaging 利用电子光谱成像技术在电子显微切片中选择性检测金颗粒的重金属对比度增强

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199809)6:3<130::AID-BIO3>3.0.CO;2-Z

Ansgar Haking, Helmut Troester, Karsten Richter, Annett Burzlaff, Herbert Spring, Michael F Trendelenburg

引用次数: 1

Real-time endoscopic fluorescence imaging for early cancer detection in the gastrointestinal tract 实时内镜荧光成像用于胃肠道早期癌症的检测

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199812)6:4<151::AID-BIO1>3.0.CO;2-G

Haishan Zeng, Alan Weiss, Richard Cline, Calum E MacAulay

{"title":"Real-time endoscopic fluorescence imaging for early cancer detection in the gastrointestinal tract","authors":"Haishan Zeng, Alan Weiss, Richard Cline, Calum E MacAulay","doi":"10.1002/1361-6374(199812)6:4<151::AID-BIO1>3.0.CO;2-G","DOIUrl":"10.1002/1361-6374(199812)6:4<151::AID-BIO1>3.0.CO;2-G","url":null,"abstract":"The utility of autofluorescence imaging for early lung cancer detection has been previously demonstrated. The aim of this work is to extend the use of real-time autofluorescence imaging to the early cancer detection in the esophagus, stomach, and colon. A prototype fluorescence imaging system for the gastrointestinal (GI) tract was developed which produces real-time video images of tissue autofluorescence. It consists of a filtered mercury arc lamp light source, two intensified charge coupled device (ICCD) cameras, a fiber optic endoscope, and a computer-based control console. The system is capable of working with three different imaging modalities: (1) conventional white light imaging mode; (2) light induced fluorescence (LIF) imaging mode based on the fluorescence imaging of two wavelength bands (green and red); and (3) light induced fluorescence and reflectance (LIFR) imaging mode based on the combination of a green band fluorescence image and a red–near-IR reflectance image. The imaging wavelength bands were selected based on in vivo fluorescence spectroscopic studies. The fluorescence images (both LIF image and LIFR image) clearly delineate the abnormal tissue areas for biopsy. Early cancer sites are better visualized under fluorescence imaging than under conventional white light examination. Initial clinical tests demonstrated the usefulness of the imaging prototype system for early cancer detection in the GI tract.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"6 4","pages":"151-165"},"PeriodicalIF":0.0,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199812)6:4<151::AID-BIO1>3.0.CO;2-G","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78138944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 75

Techniques for single molecule sequencing 单分子测序技术

Bioimaging Pub Date : 2001-05-11 DOI: 10.1002/1361-6374(199709)5:3<139::AID-BIO8>3.0.CO;2-%23

Klaus Dörre, Susanne Brakmann, Michael Brinkmeier, Kyung-Tae Han, Katja Riebeseel, Petra Schwille, Jens Stephan, Timm Wetzel, Markus Lapczyna, Michael Stuke, Raoul Bader, Michael Hinz, Hartmut Seliger, Johan Holm, Manfred Eigen, Rudolf Rigler

{"title":"Techniques for single molecule sequencing","authors":"Klaus Dörre, Susanne Brakmann, Michael Brinkmeier, Kyung-Tae Han, Katja Riebeseel, Petra Schwille, Jens Stephan, Timm Wetzel, Markus Lapczyna, Michael Stuke, Raoul Bader, Michael Hinz, Hartmut Seliger, Johan Holm, Manfred Eigen, Rudolf Rigler","doi":"10.1002/1361-6374(199709)5:3<139::AID-BIO8>3.0.CO;2-%23","DOIUrl":"https://doi.org/10.1002/1361-6374(199709)5:3<139::AID-BIO8>3.0.CO;2-%23","url":null,"abstract":"A method is described that demonstrates a new technique for rapid and high-throughput single molecule sequencing. This sequencing technique is based on the successive enzymatic degradation of fluorescently labeled single DNA molecules, and the detection and identification of the released monomer molecules according to their sequential order in a microstructured channel.The detection technique is evolved from confocal fluorescence microscopy, with two different laser sources to excite the individual mononucleotides that are either labeled with tetramethylrhodamine (TMR) or Cyanine5 (Cy5). The handling of DNA which is immobilized on carrier beads, and the detection of the cleaved monomers is performed in optically transparent and biochemically inert microstructures (glass or PMMA) with detection channels of 7 μ × 10 μm.The projected rate of sequencing is ≈100 bases min−1, dependent solely on the rate of the enzymatic DNA cleavage.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"5 3","pages":"139-152"},"PeriodicalIF":0.0,"publicationDate":"2001-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/1361-6374%28199709%295%3A3%3C139%3A%3AAID-BIO8%3E3.0.CO%3B2-%23","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137670534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0