Real-time endoscopic fluorescence imaging for early cancer detection in the gastrointestinal tract

Haishan Zeng, Alan Weiss, Richard Cline, Calum E MacAulay
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引用次数: 75

Abstract

The utility of autofluorescence imaging for early lung cancer detection has been previously demonstrated. The aim of this work is to extend the use of real-time autofluorescence imaging to the early cancer detection in the esophagus, stomach, and colon. A prototype fluorescence imaging system for the gastrointestinal (GI) tract was developed which produces real-time video images of tissue autofluorescence. It consists of a filtered mercury arc lamp light source, two intensified charge coupled device (ICCD) cameras, a fiber optic endoscope, and a computer-based control console. The system is capable of working with three different imaging modalities: (1) conventional white light imaging mode; (2) light induced fluorescence (LIF) imaging mode based on the fluorescence imaging of two wavelength bands (green and red); and (3) light induced fluorescence and reflectance (LIFR) imaging mode based on the combination of a green band fluorescence image and a red–near-IR reflectance image. The imaging wavelength bands were selected based on in vivo fluorescence spectroscopic studies. The fluorescence images (both LIF image and LIFR image) clearly delineate the abnormal tissue areas for biopsy. Early cancer sites are better visualized under fluorescence imaging than under conventional white light examination. Initial clinical tests demonstrated the usefulness of the imaging prototype system for early cancer detection in the GI tract.

实时内镜荧光成像用于胃肠道早期癌症的检测
自体荧光成像在早期肺癌检测中的应用已经得到证实。这项工作的目的是将实时自身荧光成像的使用扩展到食道、胃和结肠的早期癌症检测。研制了一种用于胃肠道的荧光成像系统原型,该系统可产生组织自身荧光的实时视频图像。它由一个滤光汞弧灯光源、两个强化电荷耦合器件(ICCD)相机、一个光纤内窥镜和一个基于计算机的控制台组成。该系统能够在三种不同的成像模式下工作:(1)传统白光成像模式;(2)基于绿、红两波段荧光成像的光诱导荧光(LIF)成像模式;(3)基于绿色波段荧光图像与红色近红外反射图像相结合的光诱导荧光与反射(LIFR)成像模式。根据体内荧光光谱研究选择成像波长波段。荧光图像(包括LIF图像和LIFR图像)清楚地描绘了活检的异常组织区域。荧光成像比常规白光检查能更好地显示早期肿瘤部位。初步临床试验证明了该成像原型系统在胃肠道早期癌症检测中的有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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