Bioimaging最新文献_第5页

Endoscopic fluorescence imaging of tissue selective lanthanide chelates 组织选择性镧系螯合物的内镜荧光成像

Bioimaging Pub Date : 1998-06-01 DOI: 10.1002/1361-6374(199806)6:2<63::AID-BIO1>3.0.CO;2-O

D. S. Hubbard, M. Houlne, G. Kiefer, K. Mcmillan, D. Bornhop

引用次数: 12

Axial resolution in the fibre-optical confocal microscope 光纤共聚焦显微镜的轴向分辨率

Bioimaging Pub Date : 1998-06-01 DOI: 10.1002/1361-6374(199806)6:2<98::AID-BIO5>3.0.CO;2-V

M. Sharma, C. Sheppard

引用次数: 10

Surface‐enhanced Raman scattering (SERS)—a new tool for single molecule detection and identification 表面增强拉曼散射(SERS)是单分子检测和鉴定的新工具

Bioimaging Pub Date : 1998-06-01 DOI: 10.1002/1361-6374(199806)6:2<104::AID-BIO6>3.0.CO;2-T

K. Kneipp, H. Kneipp, R. Manoharan, I. Itzkan, R. Dasari, M. Feld

引用次数: 65

Systematic detection of subtle spatio‐temporal patterns in time‐lapse imaging: II. Particle migrations 延时成像中细微时空模式的系统检测:2。粒子迁移

Bioimaging Pub Date : 1998-06-01 DOI: 10.1002/1361-6374(199806)6:2<71::AID-BIO2>3.0.CO;2-Q

R. Valdés-Pérez, Christopher A. Stone

引用次数: 5

An electromagnetic theory of imaging in fluorescence microscopy, and imaging in polarization fluorescence microscopy 荧光显微镜成像的电磁理论，以及偏振荧光显微镜成像

Bioimaging Pub Date : 1997-12-01 DOI: 10.1002/1361-6374(199712)5:4<205::AID-BIO4>3.3.CO;2-V

C. Sheppard, P. Török

引用次数: 49

Spatial arrangement of homologous chromosomes during anaphase in early embryos of Drosophila melanogaster studied by three-dimensional fluorescence microscopy 三维荧光显微镜研究黑腹果蝇早期胚胎后期同源染色体的空间排列

Bioimaging Pub Date : 1997-12-01 DOI: 10.1002/1361-6374(199712)5:4<183::AID-BIO2>3.3.CO;2-1

Y. Hiraoka, D. Agard, J. Sedat

{"title":"Spatial arrangement of homologous chromosomes during anaphase in early embryos of Drosophila melanogaster studied by three-dimensional fluorescence microscopy","authors":"Y. Hiraoka, D. Agard, J. Sedat","doi":"10.1002/1361-6374(199712)5:4<183::AID-BIO2>3.3.CO;2-1","DOIUrl":"https://doi.org/10.1002/1361-6374(199712)5:4<183::AID-BIO2>3.3.CO;2-1","url":null,"abstract":"Using a computer-controlled fluorescence microscope system, spatial arrangement of homologous chromosomes was analyzed in fixed and living embryos of Drosophila melanogaster at the syncytial blastoderm stage. In fixed embryos, chromosomes were stained with a DNA-specific fluorescent dye, 4′,6-diamidino-2-phenylindole; the arrangement of anaphase chromosomes was determined with their identification by high-resolution analysis. In living embryos, the behavior of chromosomes during anaphase was examined by the use of a strain carrying a long translocated chromosome as a cytological marker to identify the chromosome while the chromosomes were stained by microinjection of rhodamine-conjugated histones into the embryos. Chromosome arrangement was also examined in nuclei that had swollen artificially under anoxic conditions for better spatial separation of individual chromosomes in such nuclei. All these experiments consistently showed that homologous chromosomes were not associated with each other in syncytial blastoderm embryos of Drosophila melanogaster. Our studies also demonstrated that cytological tools greatly facilitate the dissection of nuclear structures when used in combination with imaging technology.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"118 1","pages":"183-193"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80311477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Multiparameter image cytometry: From confocal micrographs to subcellular fluorograms 多参数图像细胞术:从共聚焦显微图到亚细胞荧光图

Bioimaging Pub Date : 1997-09-01 DOI: 10.1002/1361-6374(199709)5:3<159::AID-BIO10>3.3.CO;2-X

D. Demandolx, J. Davoust

引用次数: 22

Steps towards spatially resolved single molecule detection in solution 在溶液中实现空间分辨单分子检测的步骤

Bioimaging Pub Date : 1997-09-01 DOI: 10.1002/1361-6374(199709)5:3<116::AID-BIO5>3.3.CO;2-A

H. Mathis, G. Kalusche, B. Wagner, J. McCaskill

{"title":"Steps towards spatially resolved single molecule detection in solution","authors":"H. Mathis, G. Kalusche, B. Wagner, J. McCaskill","doi":"10.1002/1361-6374(199709)5:3<116::AID-BIO5>3.3.CO;2-A","DOIUrl":"https://doi.org/10.1002/1361-6374(199709)5:3<116::AID-BIO5>3.3.CO;2-A","url":null,"abstract":"Spatially resolved single molecule detection in solution is a prerequisite for molecule tracking and provides new opportunities in single molecule manipulation, for example in a sample flow. A detector with fast timing properties and high spatial resolution is required for these purposes. We introduce a concept for spatially resolved optical single molecule detection in an epi-illuminated microscope using a novel kind of detector and a new algorithm in configurable hardware for intelligent data processing. The analysis is performed on a parallel hardware interface and includes burst detection. It can be extended to on-line spatial and temporal data analysis. This detector will be used in combination with a molecular sorter where molecules are sorted in a microstructured flow device made of silicon. In a first step towards such sorting in these microstructures, we show that a reliable detection of single molecules in silicon microstructures is possible in zero-dimensional detection volumes. The noise structure of the data is analysed in terms of Poissonian statistics and it is shown that in the smallest structure used (depth 20 μm) a signal-to-noise ratio of 40 is achieved.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"28 1","pages":"116-128"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72882377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Strategy for room temperature spectroscopy of single molecules 单分子室温光谱分析策略

Bioimaging Pub Date : 1997-09-01 DOI: 10.1002/1361-6374(199709)5:3<99::AID-BIO3>3.3.CO;2-N

T. Ha, D. Chemla, T. Enderle, S. Weiss

引用次数: 4

Techniques for single molecule sequencing 单分子测序技术

Bioimaging Pub Date : 1997-09-01 DOI: 10.1002/1361-6374(199709)5:3<139::AID-BIO8>3.3.CO;2-R

K. Dörre, S. Brakmann, M. Brinkmeier, Kyung-Tae Han, K. Riebeseel, P. Schwille, J. Stephan, T. Wetzel, M. Lapczyna, M. Stuke, R. Bader, M. Hinz, H. Seliger, J. Holm, M. Eigen, R. Rigler

{"title":"Techniques for single molecule sequencing","authors":"K. Dörre, S. Brakmann, M. Brinkmeier, Kyung-Tae Han, K. Riebeseel, P. Schwille, J. Stephan, T. Wetzel, M. Lapczyna, M. Stuke, R. Bader, M. Hinz, H. Seliger, J. Holm, M. Eigen, R. Rigler","doi":"10.1002/1361-6374(199709)5:3<139::AID-BIO8>3.3.CO;2-R","DOIUrl":"https://doi.org/10.1002/1361-6374(199709)5:3<139::AID-BIO8>3.3.CO;2-R","url":null,"abstract":"A method is described that demonstrates a new technique for rapid and high-throughput single molecule sequencing. This sequencing technique is based on the successive enzymatic degradation of fluorescently labeled single DNA molecules, and the detection and identification of the released monomer molecules according to their sequential order in a microstructured channel. \u0000 \u0000 \u0000 \u0000The detection technique is evolved from confocal fluorescence microscopy, with two different laser sources to excite the individual mononucleotides that are either labeled with tetramethylrhodamine (TMR) or Cyanine5 (Cy5). The handling of DNA which is immobilized on carrier beads, and the detection of the cleaved monomers is performed in optically transparent and biochemically inert microstructures (glass or PMMA) with detection channels of 7 μ × 10 μm. \u0000 \u0000 \u0000 \u0000The projected rate of sequencing is ≈100 bases min−1, dependent solely on the rate of the enzymatic DNA cleavage.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83293599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 60