荧光标记DNA切割过程的荧光相关光谱数值分析

Goro Nishimura, Rudolf Rigler, Masataka Kinjo
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引用次数: 12

摘要

采用荧光相关法监测t7外切酶标记的罗丹明DNA的切割过程。推导了考虑片段荧光效率的荧光相关函数,以便对双组分体系进行分析。该反应由双组分模型很好地表示,允许监测DNA的渐进切割。分析不仅得到了聚合物片段的大小,还得到了罗丹明的掺入效率及其在DNA和溶液中的量子产率之比。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Number analysis of fluorescence correlation spectroscopy for the cleaving process of fluorescence labeled DNA

The cleaving process of rhodamine labeled DNA with T7-exonuclease was monitored using the fluorescence correlation method. The fluorescence correlation function, formulated so as to take into account the fluorescence efficiency of the fragment, was derived to allow analysis of the two-component system. The reaction was well represented by the two-component model, allowing monitoring of the progressive cleavage of DNA. The analysis yielded not only the size of the polymer fragment but also the efficiency of the incorporation of rhodamine and the ratio of its quantum yield in DNA and in solution.

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