单分子室温光谱分析策略

T Ha, D S Chemla, Th Enderle, S Weiss
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引用次数: 0

摘要

室温下单分子的激光诱导荧光(LIF)在分子的光破坏寿命内表现出丰富的可逆和不可逆转变动力学。与系综研究相反,当荧光信号被及时监测时,这些跃迁可以直接解决。我们提出了一种策略,并描述了对大量单个分子进行光谱研究所需的仪器。它是基于一个计算机控制的光学系统,该系统自动和快速地定位单个分子在共聚焦显微镜的激发体积,并随后进行光谱测量。给出了这种光谱的例子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Strategy for room temperature spectroscopy of single molecules

Laser-induced fluorescence (LIF) from single molecules at room temperature displays rich dynamics of reversible and irreversible transitions within the photodestruction lifetime of the molecule. In contrast to ensemble studies, these transitions are directly resolved when the fluorescence signal is monitored in time. We present a strategy and describe the instrumentation needed for spectroscopic studies on a large number of individual molecules. It is based on a computer controlled optical system which automatically and rapidly positions single molecules in the excitation volume of a confocal microscope and subsequently performs spectroscopic measurements. Examples for such spectroscopies are presented.

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