Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects最新文献_第8页

A rapid method for the separation of nucleic acid bases 一种快速分离核酸碱基的方法

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90258-0

D.P. Holdgate, T.W. Goodwin

引用次数: 17

Influence of Hg(II) on the metachromatic complexes of deoxyribonucleic acid with acridine orange 汞(II)对脱氧核糖核酸与吖啶橙异色络合物的影响

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90262-2

J. Koudelka, V. Kleinwächter, G. Blažíček

引用次数: 4

Effect of mitomycin C on the synthesis of messenger RNA in Escherichia coli 丝裂霉素C对大肠杆菌信使RNA合成的影响

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90270-1

I. Smith-Kielland

引用次数: 14

Fractionation of transforming deoxyribonucleic acid of Bacillus subtilis with the methylated-albumin column 甲基化白蛋白柱分离枯草芽孢杆菌转化脱氧核糖核酸

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90264-6

Hiuga Saito, Yukito Masamune

引用次数: 11

Properties of a ribonucleic acid synthesizing system in cell-free extracts of tobacco leaves 烟叶无细胞提取物中核糖核酸合成体系的性质

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90243-9

J. Semal , D. Spencer , Y.T. Kim, S.G. Wildman

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引用次数: 34

The incorporation of [3H]cytidine into ribonucleic acid of liver nuclei of young and old rats [3H]胞苷在幼龄和老年大鼠肝核核糖核酸中的掺入

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90245-2

Harvey V. Samis Jr., V.J. Wulff, J.A. Falzone Jr.

{"title":"The incorporation of [3H]cytidine into ribonucleic acid of liver nuclei of young and old rats","authors":"Harvey V. Samis Jr., V.J. Wulff, J.A. Falzone Jr.","doi":"10.1016/0926-6550(64)90245-2","DOIUrl":"10.1016/0926-6550(64)90245-2","url":null,"abstract":"<div>The ribonucleic acid of rat-liver nuclei labeled with [3H]cytidine and harvested from liver homogenates using a phenol-NaCl mixture may be fractionated by successive extractions at temperatures between 20 and 75°, using a phenol-NaCl extraction medium. Successive extractions at any one temperature produce extracts whose ribonucleic acid content decreases markedly. The analysis of the specific activity of extracted nuclear ribonucleic acid indicates two fractions: (1) a relatively low specific-activity fraction obtained at 25, 40 and 50°; and (2) a relatively high specific-activity fraction obtained at 65 and 75°, temperatures at which measurable amounts of deoxyribonucleic acid are also extracted.It is demonstrated that InCl3 at appropriate concentration and ionic strength, effectively precipitates all soluble ribonucleic acid as well as all deoxyribonucleic acid as the indic nucleates. The indic nucleates are solubilized in 0.38 N KOH which results in the precipitation of In (OH)3 in an exchange reaction that is complete in about 2 h at room temperature. In this way the ribonucleic acid may be hydrolysed to constituent nucleotides and the unhydrolysed deoxyribonucleic acid may be recovered by perchloric acid precipitation.The results of extraction experiments with nuclei harvested from liver of young and old adult rats indicate: (1) the specific activity of ribonucleic acid extracted from “old” liver nuclei is consistently higher than that extracted from “young” nuclei; (2) the amount of ribonucleic acid extracted from “young” liver nuclei is greater than that extracted from “old” liver nuclei; and (3) the amounts of deoxyribonucleic acid extracted from “young” and “old” liver nuclei are approximately equal.</div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 2","pages":"Pages 223-232"},"PeriodicalIF":0.0,"publicationDate":"1964-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90245-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23789595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Solubility and comparison of protein-deoxyribonucleic acid complexes 蛋白质-脱氧核糖核酸复合物的溶解度和比较

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90263-4

B.H.J. Hofstee

引用次数: 26

Ribonucleoprotein particles from storage tissue of mature seeds 成熟种子贮藏组织中的核糖核蛋白颗粒

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90266-X

Marshall Phillips

引用次数: 4

The effect of DNA-histone interactions on the biosynthesis of DNA in vitro DNA-组蛋白相互作用对体外DNA生物合成的影响

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90251-8

Lubomir S. Hnilica, Daniel Billen

引用次数: 37

neo-Guanylic acid produced by the action of acid on ribonucleic acid 由酸作用于核糖核酸而产生的新鸟苷酸

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90260-9

W.F. Hemmens

引用次数: 3