Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects最新文献_第6页

Purification and properties of lysyl- and methionyl-soluble ribonucleic acid synthetases from wheat germ 小麦胚芽中赖氨酸和蛋氨酸可溶性核糖核酸合成酶的纯化及其性质

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90072-6

Esam Moustafa

引用次数: 18

Dielectric properties of deoxyribonucleic acid solutions in salt water, formamide and urea 脱氧核糖核酸溶液在盐水、甲酰胺和尿素中的介电性能

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90085-4

Guy Mesnard, Dane Vasilescu

引用次数: 4

Studies on the nucleotide arrangement in deoxyribonucleic acids IX. Selective degradation of pyrimidine deoxyribonucleotides 脱氧核糖核酸中核苷酸排列的研究IX。嘧啶脱氧核糖核苷酸的选择性降解

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90076-3

Alfred Temperli , Hans Türler, Peter Rüst, Arlette Danon, Erwin Chargaff

{"title":"Studies on the nucleotide arrangement in deoxyribonucleic acids IX. Selective degradation of pyrimidine deoxyribonucleotides","authors":"Alfred Temperli , Hans Türler, Peter Rüst, Arlette Danon, Erwin Chargaff","doi":"10.1016/0926-6550(64)90076-3","DOIUrl":"10.1016/0926-6550(64)90076-3","url":null,"abstract":"<div>The study of the sequence of purine nucleotides in deoxyribonucleic acids requires the availability of suitable preparations of apyrimidinic acid, i.e., of polydeoxyribophosphate chains retaining the purines of the parent DNA in unchanged sequence and proportions. For an understanding of the degradation of DNA a detailed knowledge and a complete balance of the hydrazinolysis of pyrimidine deoxyribomononucleotides were required.The course and the rate of the degradation by hydrazine of thymidylic and deoxycytidylic acids were studied under a variety of conditions. These nucleotides, when treated with anhydrous hydrazine, give rise to stoichiometric amounts of (a) urea; (b) the hydrazone of 2-deoxyribophosphate; and (c) a heterocyclic compound. The latter is 4-methyl-5-pyrazolone in the case of thymidylic acid, and 3(5)-amino-pyrazole in the case of deoxycytidylic acid. The hydrazone of the sugar phosphate is cleaved quantitatively with benzaldehyde to yield 2-deoxyribophosphate and benzalazine. The various breakdown products were identified by comparison with authentic preparations.The first-order reaction of the degradation with anhydrous hydrazine is completed at 60° within 1.5 h for deoxycytidylic acid and within 4 h for thymidylic acid. At 40°, the time required for completion is about 4 times as long.Other orienting experiments demonstrated that purine deoxyribonucleotides withstand the treatment with hydrazine; that aqueous hydrazine solutions at various pH values effect a slower and irregular degradation; and that the replacement of hydrazine by monosubstituted hydrazine derivatives, such as phenyl- or methylhydrazine does not yield the desired results.</div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 3","pages":"Pages 462-476"},"PeriodicalIF":0.0,"publicationDate":"1964-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90076-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23806313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Deoxyribonucleic acid from the chloroplasts of Euglena gracilis 细叶茅叶绿体中的脱氧核糖核酸

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90077-5

George Brawerman, J.M. Eisenstadt

{"title":"Deoxyribonucleic acid from the chloroplasts of Euglena gracilis","authors":"George Brawerman, J.M. Eisenstadt","doi":"10.1016/0926-6550(64)90077-5","DOIUrl":"10.1016/0926-6550(64)90077-5","url":null,"abstract":"<div><ul><li>1.1. DNA preparations from Euglena chloroplasts were found to contain a major component with a density of 1.684 in addition to the nuclear component with a density of 1.708. Partial lysis of the chloroplasts resulted in the removal of the nuclear component. A component with a density of approx. 1.692 was also associated with the chloroplast preparations.</li><li>2.2. The temperature of melting of the chloroplast DNA was 78–80°, as compared to 89–91° for the nuclear DNA.</li><li>3.3. Nucleotide analysis of the chloroplast DNA indicates an overall G+C content of about 25 mole%. The 5-methylcytosine of Euglena DNA is concentrated in the nuclear material.</li><li>4.4. The amount of DNA per chloroplast appears to be about the same as that in an Escherichia coli cell.</li><li>5.5. The nucleotide composition of the ribosomal RNA of Euglena chloroplasts seems to be influenced by that of the chloroplast DNA.</li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 3","pages":"Pages 477-485"},"PeriodicalIF":0.0,"publicationDate":"1964-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90077-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23806314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 99

Biosynthesis of bacitracin and protein 杆菌肽和蛋白质的生物合成

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90086-6

Neal Cornell, John E. Snoke

引用次数: 16

Activation de la synthèse acellulaire de l'hémoglobine par l'acide ribonucléique 核糖核酸激活细胞中血红蛋白的合成

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90079-9

J Kruh, J.C Dreyfus, G Schapira

引用次数: 3

Isolation and analysis of RNA formed by cell-free extracts from tobacco leaves 烟草叶片无细胞提取物RNA的分离与分析

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90244-0

R.H. Moyer, R.A. Smith, J. Semal , Y.T. Kim

引用次数: 6

Mechanism of inactivation of bacteriophages by metals 金属使噬菌体失活的机制

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90249-X

Nobuto Yamamoto , C.W. Hiatt , Wolfgang Haller

引用次数: 38

Subunit structure of calf-thymus deoxyribonucleic acid from absorption curves of thermal denaturation 从热变性吸收曲线看小牛胸腺脱氧核糖核酸的亚基结构

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90269-5

Ladislav Pivec, Jaroslav Šponar, Zora Šormová

引用次数: 7

Existence de “rescue” pour l'activité transformante d'un acide désoxyribonucléique après irradiation X X辐照后脱氧核糖核酸转化活性的“救援”存在

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-10-16 DOI: 10.1016/0926-6550(64)90252-X

Nicole Rebeyrotte

引用次数: 6