小麦胚芽中赖氨酸和蛋氨酸可溶性核糖核酸合成酶的纯化及其性质

Esam Moustafa
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引用次数: 18

摘要

赖氨酸-s-RNA合成酶(l-赖氨酸:s-RNA连接酶(AMP), EC 6.1.1.6)和蛋氨酸-s-RNA合成酶(l-蛋氨酸:s-RNA连接酶(AMP), EC 6.1.1.10)从小麦胚芽中分别纯化了约600倍和160倍。这两种酶催化氨基酸依赖的atp -焦磷酸交换以及氨基酸与s-RNA的结合。赖氨酸酶的最适pH为6.8 ~ 7.1,蛋氨酸酶的最适pH为8.1 ~ 8.5。这两种酶都被对羟基氨基汞苯甲酸酯强烈抑制,并被含巯基的化合物恢复活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Purification and properties of lysyl- and methionyl-soluble ribonucleic acid synthetases from wheat germ

Lysyl-s-RNA synthetase (l-lysine: s-RNA ligase (AMP), EC 6.1.1.6) and methionyl-s-RNA-synthetase (l-methionine: s-RNA ligase (AMP), EC 6.1.1.10) were purified from wheat germ about 600-fold and 160-fold, respectively. The two enzymes catalyse amino acid-dependent ATP-pyrophosphate exchange as well as the incorporation of amino acids into s-RNA. The pH optimum of lysine enzyme is in the range 6.8–7.1 and that of methionine enzyme in the range 8.1–8.5. Both enzymes are strongly inhibited by p-hydroxymercuribenzoate and the activity is restored by sulfhydryl group-containing compounds.

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