{"title":"细叶茅叶绿体中的脱氧核糖核酸","authors":"George Brawerman, J.M. Eisenstadt","doi":"10.1016/0926-6550(64)90077-5","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. DNA preparations from Euglena chloroplasts were found to contain a major component with a density of 1.684 in addition to the nuclear component with a density of 1.708. Partial lysis of the chloroplasts resulted in the removal of the nuclear component. A component with a density of approx. 1.692 was also associated with the chloroplast preparations.</p></span></li><li><span>2.</span><span><p>2. The temperature of melting of the chloroplast DNA was 78–80°, as compared to 89–91° for the nuclear DNA.</p></span></li><li><span>3.</span><span><p>3. Nucleotide analysis of the chloroplast DNA indicates an overall G+C content of about 25 mole%. The 5-methylcytosine of Euglena DNA is concentrated in the nuclear material.</p></span></li><li><span>4.</span><span><p>4. The amount of DNA per chloroplast appears to be about the same as that in an <em>Escherichia coli</em> cell.</p></span></li><li><span>5.</span><span><p>5. The nucleotide composition of the ribosomal RNA of Euglena chloroplasts seems to be influenced by that of the chloroplast DNA.</p></span></li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 3","pages":"Pages 477-485"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90077-5","citationCount":"99","resultStr":"{\"title\":\"Deoxyribonucleic acid from the chloroplasts of Euglena gracilis\",\"authors\":\"George Brawerman, J.M. Eisenstadt\",\"doi\":\"10.1016/0926-6550(64)90077-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. DNA preparations from Euglena chloroplasts were found to contain a major component with a density of 1.684 in addition to the nuclear component with a density of 1.708. Partial lysis of the chloroplasts resulted in the removal of the nuclear component. A component with a density of approx. 1.692 was also associated with the chloroplast preparations.</p></span></li><li><span>2.</span><span><p>2. The temperature of melting of the chloroplast DNA was 78–80°, as compared to 89–91° for the nuclear DNA.</p></span></li><li><span>3.</span><span><p>3. Nucleotide analysis of the chloroplast DNA indicates an overall G+C content of about 25 mole%. The 5-methylcytosine of Euglena DNA is concentrated in the nuclear material.</p></span></li><li><span>4.</span><span><p>4. The amount of DNA per chloroplast appears to be about the same as that in an <em>Escherichia coli</em> cell.</p></span></li><li><span>5.</span><span><p>5. The nucleotide composition of the ribosomal RNA of Euglena chloroplasts seems to be influenced by that of the chloroplast DNA.</p></span></li></ul></div>\",\"PeriodicalId\":100173,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"volume\":\"91 3\",\"pages\":\"Pages 477-485\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-11-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6550(64)90077-5\",\"citationCount\":\"99\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926655064900775\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064900775","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Deoxyribonucleic acid from the chloroplasts of Euglena gracilis
1.
1. DNA preparations from Euglena chloroplasts were found to contain a major component with a density of 1.684 in addition to the nuclear component with a density of 1.708. Partial lysis of the chloroplasts resulted in the removal of the nuclear component. A component with a density of approx. 1.692 was also associated with the chloroplast preparations.
2.
2. The temperature of melting of the chloroplast DNA was 78–80°, as compared to 89–91° for the nuclear DNA.
3.
3. Nucleotide analysis of the chloroplast DNA indicates an overall G+C content of about 25 mole%. The 5-methylcytosine of Euglena DNA is concentrated in the nuclear material.
4.
4. The amount of DNA per chloroplast appears to be about the same as that in an Escherichia coli cell.
5.
5. The nucleotide composition of the ribosomal RNA of Euglena chloroplasts seems to be influenced by that of the chloroplast DNA.
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