Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects最新文献_第4页

2-Amino-1,4-naphthoquinone as a model compound for actinomycins 2-氨基-1,4-萘醌作为放线菌素的模型化合物

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90014-3

R.Bruce Martin

引用次数: 2

The separation and partial purification of aminoacyl-RNA synthetases from Escherichia coli 大肠杆菌中氨基酰基rna合成酶的分离和部分纯化

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90001-5

D.J. McCorquodale

引用次数: 8

Mechanism of action of glucose and pyrimidine nucleosides on [14C]formate utilization by Ehrlich ascites cells 葡萄糖和嘧啶核苷对埃利希腹水细胞利用[14C]甲酸盐的作用机制

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90065-9

Annette Herscovics, R.M. Johnstone

{"title":"Mechanism of action of glucose and pyrimidine nucleosides on [14C]formate utilization by Ehrlich ascites cells","authors":"Annette Herscovics, R.M. Johnstone","doi":"10.1016/0926-6550(64)90065-9","DOIUrl":"10.1016/0926-6550(64)90065-9","url":null,"abstract":"<div><ul><li>1.1. When Ehrlich ascites cells are incubated in the presence of glucose, uridine, or cytidine, the intracellular concentration of free glutamate is greatly increased. The specific activity of [14C]glutamate formed from [14C]glucose is decreased 50% in the presence of 1 mM uridine.</li><li>2.2. [14C]Formate incorporation into both proteins and nucleic acids of Ehrlich ascites cells is greatly stimulated by glucose, uridine, glutamine and glutamate.</li><li>3.3. Methionine sulphoximine completely prevents the stimulations of formate utilization by glucose and uridine. These effects of methionine sulphoximine are reversed by glutamine.</li><li>4.4. It is suggested that the increased intracellular concentration of glutamate in the presence of exogenous glucose, uridine or glutamate leads to an increased availability of glutamine necessary for the synthesis of purine nucleotides and proteins. The availability of glutamate appears to be limiting for amino acid incorporation into proteins.</li><li>5.5. Exogenous [14C]glutamate is very poorly transported by Ehrlich ascites cells. After 1 h of incubation, a ratio of 0.3 for the distribution of radioactivity between cells and medium is obtained.</li><li>6.6. The incorporation of [14C]formate into free serine is stimulated 20–40-fold by glucose, uridine and cytidine. [14C]Formate incorporation into serine is greater under anaerobic conditions than under aerobic conditions.</li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90065-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23806302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Synthesis of pseudouridine 5′-diphosphate glucuronic acid 假尿嘧啶5′-二磷酸葡萄糖醛酸的合成

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90083-0

Mikio Honjo, Yoshiyasu Furukawa, Yoshio Kanai

引用次数: 9

Radiosensitization of pneumococcus cells and deoxyribonucleic acid to ultraviolet light and X-rays by incorporated 5-bromodeoxyuridine 合并5-溴脱氧尿苷使肺炎球菌细胞和脱氧核糖核酸对紫外线和x射线的放射增敏

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90084-2

Franklin Hutchinson

引用次数: 11

Thermal denaturation of deoxyribonucleic acid-acridine orange complexes 脱氧核糖核酸-吖啶橙配合物的热变性

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90088-X

Vladimír Kleinwächter , Jaromír Koudelka

引用次数: 41

Nucleotide composition and arrangement of soluble RNA in posterior silkgland 后丝腺可溶性RNA的核苷酸组成及排列

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90073-8

Kin-Ichiro Miura , Keiko Matsuzaki

引用次数: 11

Nuclease-resistant sequences in ultraviolet-irradiated deoxyribonucleic acid 紫外辐照脱氧核糖核酸的抗核酸酶序列

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90075-1

R.B. Setlow, W.L. Carrier, F.J. Bollum

引用次数: 66

Patterns of growth and α-amylase activity in the developing chick pancreas 发育中的鸡胰腺生长模式及α-淀粉酶活性

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90080-5

R.G. Kulka, D. Duksin

{"title":"Patterns of growth and α-amylase activity in the developing chick pancreas","authors":"R.G. Kulka, D. Duksin","doi":"10.1016/0926-6550(64)90080-5","DOIUrl":"10.1016/0926-6550(64)90080-5","url":null,"abstract":"<div><ul><li>1.1. Developmental changes in total pancreatic protein, DNA, RNA and α-amylase (α-1,4-glucan 4-glucanohydrolase, EC 3.2.1.1) activity were studied in chick embryos aged 10–20 days and in 1–5-day-old chicks (developmental age 21–25 days).</li><li>2.2. The protein content of the pancreas increases rapidly from 10 to 13 days, more slowly from 13 to 18 days, very rapidly from 19 to 22 days and again more slowly from 22 to 25 days.</li><li>3.3. A virtually constant concentration of RNA per mg of protein is observed from 13 to 25 days of development. On the other hand, the concentration of DNA per mg of protein, which is constant from 13 to 18 days, falls sharply between 18 and 21 days.</li><li>4.4. The specific activity of amylase remains constant from 11 to 16 days of development and increases about 8-fold from 16 to 22 days, after which it falls. At its maximum concentration (22–23 days) amylase constitutes about 12% of the total pancreatic protein.</li><li>5.5. It seems significant that the steepest rise in amylase activity between 18 and 22 days coincides with the period of rapid growth and increase in cell size which overlaps the time of hatching.</li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90080-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23806316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Some new applications of micrococcal nuclease to the elucidation of the structure of nucleic acids 微球菌核酸酶在核酸结构解析中的一些新应用

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-11-15 DOI: 10.1016/0926-6550(64)90074-X

Monique De Meuron-Landolt, Michel Privat de Garilhe

{"title":"Some new applications of micrococcal nuclease to the elucidation of the structure of nucleic acids","authors":"Monique De Meuron-Landolt, Michel Privat de Garilhe","doi":"10.1016/0926-6550(64)90074-X","DOIUrl":"10.1016/0926-6550(64)90074-X","url":null,"abstract":"<div>In order to obtain more information on the specificity of micrococcal nuclease (EC 3.1.4.7), three series of substances were investigated: <ul><li>1.1. The series of polypyrimidine nucleotides terminated at both ends by phosphoryl groups obtained by controlled hydrolysis of DNA.</li><li>2.2. Polythymidylic acids obtained by chemical synthesis.</li><li>3.3. Three naturally occuring deoxyribonucleic acids.</li></ul>Using catalytic amounts of highly purified enzyme, it was shown that tri- and tetranucleotides are attacked with the liberation of the 3′-terminal nucleoside and (or) nucleotide in a stepwise manner. With pentanucleotides, exonucleolytic and endonucleolytic mechanisms of hydrolysis occur at the same rate. The fact that the enzyme attacks preferentially thymine and adenine linkages has been confirmed by using deoxyribonucleic acids containing different adenine-thymine ratios.</div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90074-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23806311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12