大肠杆菌中氨基酰基rna合成酶的分离和部分纯化

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI:10.1016/0926-6550(64)90001-5

D.J. McCorquodale

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引用次数: 8

摘要

本文描述了大肠杆菌中色氨酸、酪氨酸、蛋氨酸、异亮氨酸、半胱氨酸、缬氨酸和亮氨酸特异性氨基酸- rna合成酶(氨基酸- rna连接酶，EC类6.1.1)的分离和部分纯化的简单方法。分离是通过在取代的多糖树脂上对无颗粒细菌提取物进行层析实现的。

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The separation and partial purification of aminoacyl-RNA synthetases from Escherichia coli

A simple method for the separation and partial purification of aminoacyl-RNA synthetases (aminoacid-RNA ligases, EC class 6.1.1) specific for tryptophan, tyrosine, methionine, isoleucine, cysteine, valine and leucine from Escherichia coli is described. The separations are achieved by chromatography of particle-free bacterial extracts on substituted polysaccharide resins.

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects

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