Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides最新文献_第6页

Characterization and measurement of acid mucopolysaccharides in tissues 组织中酸性粘多糖的表征和测定

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90039-4

Diana S. Trundle , George V. Mann

引用次数: 10

Hyaluronidase assay using a chondroitinsulphate substrate 用软骨素胰岛素底物测定透明质酸酶

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90027-8

J.M. Bowness

{"title":"Hyaluronidase assay using a chondroitinsulphate substrate","authors":"J.M. Bowness","doi":"10.1016/0926-6534(65)90027-8","DOIUrl":"10.1016/0926-6534(65)90027-8","url":null,"abstract":"<div><ul><li>1.1. A spectrophotometric titration procedure for the estimation of chondroitin-sulphate using buffered toluidine blue has been developed. The titration readings are decreased by the action of hyaluronidase on chondroitinsulphate. Using this principle hyaluronidase has been assayed in amounts of 0.025–0.25 USP units. This range of activities corresponds with the depolymerisation of chondroitinsulphate at the rate of 45–450, μμequiv. glucuronic acid per min per 0.1 ml test solution.</li><li>2.2. The standard curves obtained with three different preparations of chondroitinsulphate differed little.</li><li>3.3. The assay is performed in the presence of 0.33 mM mercuric chloride. Ferric and ferrous salts at the same concentration inhibit the hyaluronidase activity. Crude serum albumin inhibits the activity slightly at the concentration present in blood.</li><li>4.4. The procedure has been shown to be applicable to the assay of hyaluronidase activity in fractions from blood serum and in serum itself.</li><li>5.5. The optimum pH of testicular hyaluronidase in the procedure and the nature of the products of the enzymic reaction have been studied.</li></ul></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90027-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23850062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Isolation of fucose-rich glycopeptides from normal urine 从正常尿液中分离富焦糖肽

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90029-1

A. Lundblad

引用次数: 15

Separation of the M and N blood-group antigens of the human erythrocyte 人红细胞M、N血型抗原的分离

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90030-8

G.M.W. Cook, E.H. Eylar

引用次数: 86

Effect of vitamin A on the activity of arylsulfatase and β-glucuronidase of rat tissues 维生素A对大鼠组织芳基硫酸酯酶和β-葡萄糖醛酸酶活性的影响

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90036-9

Lichu Hsu , A.L. Tappel

引用次数: 15

Incorporation of [I-14C]glucosamine into mucopolysaccharides of rat connective tissue [I-14C]葡萄糖胺掺入大鼠结缔组织粘多糖

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90034-5

Betty N. White, M.R. Shetlar , H.M. Shurley, J.A. Schilling

{"title":"Incorporation of [I-14C]glucosamine into mucopolysaccharides of rat connective tissue","authors":"Betty N. White, M.R. Shetlar , H.M. Shurley, J.A. Schilling","doi":"10.1016/0926-6534(65)90034-5","DOIUrl":"https://doi.org/10.1016/0926-6534(65)90034-5","url":null,"abstract":"<div><ul><li>1.1. Incorporation of [I-14C]glucosamine into acid mucopolysaccharides was studied in connective tissue formed in stainless steel wire mesh cylinders implanted subcutaneously into adult rats. Liver, serum, and the fluids found within cylinders were also investigated.</li><li>2.2. High levels of non-dialyzable radioactivity were observed in tissue when labelled glucosamine was injected directly into the area of connective tissue proliferation within cylinders.</li><li>3.3. Incorporation of [I-14C]glucosamine into connective tissue was not adversely affected by removal of the animal's liver; only a small amount of radioactivity was found in serum proteins of these animals.</li><li>4.4. Incorporation of glucosamine by connective tissue slices was demonstrated by studies in vitro.</li><li>5.5. Mucopolysaccharides with high specific radioactivity, prepared from connective tissue labelled in vivo or in vitro, had staining characteristics and electrophoretic mobility of hyaluronic acid and chondroitin sulfate. Glucosamine and galactosamine, isolated from connective tissue, were found to be radioactive. It may be concluded that glucosamine can be directly incorporated into mucopolysaccharides at the site of connective tissue formation.</li></ul></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90034-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91634969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Studies on glycoproteins XI. The O-glycosidic linkage of N-acetylgalactosamine to seryl and threonyl residues in ovine submaxillary gland glycoprotein 糖蛋白的研究羊颌下腺糖蛋白中n -乙酰半乳糖胺与丝氨酸和苏氨酸残基的o -糖苷键

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90031-X

R. Carubelli , V.P. Bhavanandan, A. Gottschalk

{"title":"Studies on glycoproteins XI. The O-glycosidic linkage of N-acetylgalactosamine to seryl and threonyl residues in ovine submaxillary gland glycoprotein","authors":"R. Carubelli , V.P. Bhavanandan, A. Gottschalk","doi":"10.1016/0926-6534(65)90031-X","DOIUrl":"10.1016/0926-6534(65)90031-X","url":null,"abstract":"<div>Ovine submaxillary glycoprotein (OSM) was digested exhaustively with pronase and the sialoglycopeptides separated by gel filtration. By subsequent neuraminidase (EC 3.2.1.18) treatment and further purification, glycopeptides of average molecular weight 660 and containing 50% of the total galactosamine of OSM were obtained. Serine and threonine were the only amino acids with a functional group in the side chain present in a concentration high enough to accommodate all hexosamine residues. Treatment of the glycopeptides with 0.5 N NaOH at 0° or 22° resulted in the release of N-acetylgalactosamine coincident with the loss of an equimolecular amount of hydroxyamino acids. Most likely the carbohydrate was released by the mechanism of β-carbonyl elimination. Some evidence for the formation of an unsaturated compound, presumably α-aminoacrylic acid, was afforded spectrophotometrically.It is concluded that in OSM at least 50% of the prosthetic groups are joined glycosidically to the hydroxyl groups of serine and threonine.When native OSM was treated at pH 8.0 and 42° for 120 h, about one-third of the prosthetic groups was released. After this treatment the isolated, non-dialyzable residual glycoprotein reacted strongly positive in the Warren test. The mechanism of the reaction is discussed.</div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90031-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23850066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 96