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Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides最新文献

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The dissolution and fractionation of bovine sclera 牛巩膜的溶解与分离
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90005-9
Jerome Rotstein M.D. , Jules Seltzer M.S.
{"title":"The dissolution and fractionation of bovine sclera","authors":"Jerome Rotstein M.D. ,&nbsp;Jules Seltzer M.S.","doi":"10.1016/0926-6534(65)90005-9","DOIUrl":"10.1016/0926-6534(65)90005-9","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. A method for dissolving and fractionating bovine sclera has been described. High speed homogenization, utilizing double water extraction, separates out the protein-polysaccharide from the sclera. The residue is disolved with pepsin (EC 3.4.4.1) and trypsin (EC 3.4.4.4) and fractionated by dialysis and cold alcohol precipitation.</p></span></li><li><span>2.</span><span><p>2. Chondroitin sulfates A, B and C are found as protein-polysaccharide complexes in bovine sclera. No keratosulfate was found in the tissue.</p></span></li><li><span>3.</span><span><p>3. No <em>N</em>-acetyl neuraminic acid is present in bovine sclera.</p></span></li><li><span>4.</span><span><p>4. Comparison of the fractions of the bovine sclera and bovine nasal cartilage reveals that the tissues are different in composition and structure.</p></span></li></ul></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90005-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15394420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
The isolation of a dermatan sulphate-protein complex from bovine heart valves 牛心脏瓣膜中硫酸皮肤蛋白复合物的分离
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90017-5
B.P. Toole, D.A. Lowther
{"title":"The isolation of a dermatan sulphate-protein complex from bovine heart valves","authors":"B.P. Toole,&nbsp;D.A. Lowther","doi":"10.1016/0926-6534(65)90017-5","DOIUrl":"10.1016/0926-6534(65)90017-5","url":null,"abstract":"","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90017-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15329925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Some hydrodynamic properties of native and modified serum orosomucoids 天然和改良血清口体样体的一些流体动力学性质
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90012-6
Anatoly Bezkorovainy
{"title":"Some hydrodynamic properties of native and modified serum orosomucoids","authors":"Anatoly Bezkorovainy","doi":"10.1016/0926-6534(65)90012-6","DOIUrl":"https://doi.org/10.1016/0926-6534(65)90012-6","url":null,"abstract":"<div><p>Human and bovine serum orosomucoids and their neuraminidase (EC 3.2.1.18) treated counterparts were characterized with respect to their sedimentation, diffusion, viscosity, and optical rotatory properties at pH 7 in cacodylate buffer. The human and bovine orosomucoids had molecular weights of 41600 and 37000 respectively by the sedimentation diffusion method. Calculations involving the frictional coefficient and viscosity increment indicated that the two orosomucoids were highly hydrated prolate shaped molecules with an axial ratio of 2.5. The removal of sialic acid caused a loss of the water of hydration with an increase of the axial ratio to 5 and no loss of helical structure.</p></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90012-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90001645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
The effect of sulfate deficiency on the mechanical strength of the rat's aorta 硫酸盐缺乏对大鼠主动脉机械强度的影响
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90016-3
R.Glenn Brown, Grace M. Button, John T. Smith
{"title":"The effect of sulfate deficiency on the mechanical strength of the rat's aorta","authors":"R.Glenn Brown,&nbsp;Grace M. Button,&nbsp;John T. Smith","doi":"10.1016/0926-6534(65)90016-3","DOIUrl":"10.1016/0926-6534(65)90016-3","url":null,"abstract":"","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90016-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15329924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Composition of mucopeptide from the spores of Bacillus coagulans 凝固芽孢杆菌孢子中粘肽的组成
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90010-2
A.D. Warth
{"title":"Composition of mucopeptide from the spores of Bacillus coagulans","authors":"A.D. Warth","doi":"10.1016/0926-6534(65)90010-2","DOIUrl":"10.1016/0926-6534(65)90010-2","url":null,"abstract":"<div><p>Spore mucopeptide was prepared by autolysis of the cortical structure of <em>Bacillus coagulans</em> spores. Analysis showed that it contained glucosamine, muramic acid, <span>L</span>-alanine, <span>D</span>-glutamic acid, <em>meso</em>-diaminopimelic acid and <span>D</span>-alanine in the molecular proportions 2.8:3:1.6:1.0:1.0:1.0. About 80% of the diaminopimelic acid residues had one amino group which reacted with fluorodinitrobenzene. Alanine in trace amounts was the only N-terminal amino acid detected. The presence of most of the expected free carboxyl and amino groups was confirmed by titration studies. Not all the amino sugar appeared to be N-acetylated. The results are consistent with the proposition that the spore cortical structure consists of a loosely cross-linked matrix bearing a considerable excess of carboxyl groups.</p></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90010-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"16937326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Isolation and identification of a lactose sulphate ester from rat mammary gland 大鼠乳腺中一种硫酸乳糖酯的分离鉴定
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90018-7
Hector S. Barba, Ranwel Caputto
{"title":"Isolation and identification of a lactose sulphate ester from rat mammary gland","authors":"Hector S. Barba,&nbsp;Ranwel Caputto","doi":"10.1016/0926-6534(65)90018-7","DOIUrl":"10.1016/0926-6534(65)90018-7","url":null,"abstract":"","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90018-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"16937330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Hexosamine and acid glycosaminoglycans in human teeth 人类牙齿中的己糖胺和酸性糖胺聚糖
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90004-7
Richard D. Clark , J.Graham Smith Jr. , Eugene A. Davidson
{"title":"Hexosamine and acid glycosaminoglycans in human teeth","authors":"Richard D. Clark ,&nbsp;J.Graham Smith Jr. ,&nbsp;Eugene A. Davidson","doi":"10.1016/0926-6534(65)90004-7","DOIUrl":"10.1016/0926-6534(65)90004-7","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Human tooth dentine-cementum and enamel contain 0.08% and 0.03% hexosamine, respectively, with approximately equivalent amounts of glucosamine and galactosamine.</p></span></li><li><span>2.</span><span><p>2. Chondroitin 4-sulfate and/or chondroitin 6-sulfate are present in dentine-cementum and enamel of human teeth with smaller amounts of hyaluronic acid. There is apparently no dermatan or keratan sulfate.</p></span></li><li><span>3.</span><span><p>3. Chondroitin 6-sulfate is the major acid glycosaminoglycan in the dentine-cementum of human teeth.</p></span></li></ul></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90004-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15329920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 69
Acid mucopolysaccharide composition of human-heart valve 人体心脏瓣膜的酸性粘多糖组成
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90007-2
Shinichiro Torii , Reza I. Bashey , Komei Nakao
{"title":"Acid mucopolysaccharide composition of human-heart valve","authors":"Shinichiro Torii ,&nbsp;Reza I. Bashey ,&nbsp;Komei Nakao","doi":"10.1016/0926-6534(65)90007-2","DOIUrl":"10.1016/0926-6534(65)90007-2","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The content and fractional distribution of acid mucopolysaccharides in normal human-heart valves were studied.</p></span></li><li><span>2.</span><span><p>2. The acid mucopolysaccharide content was significantly different in the aortic, mitral and tricuspid valves in the young age group. The aortic valve contained the highest amount of acid mucopolysaccharide and the tricuspid had the least.</p></span></li><li><span>3.</span><span><p>3. The content of acid mucopolysaccharide decreased with age in these three valves. The decrease was most marked in the aortic valve and the tricuspid showed the least change.</p></span></li><li><span>4.</span><span><p>4. Three kinds of acid mucopolysaccharide, namely hyaluronic acid, chondroitin sulfate B and chondroitin sulfate A or C were found in these valves. Hyaluronic acid was the major component constituting approx. 60% of the total acid mucopolysaccharide.</p></span></li><li><span>5.</span><span><p>5. There was no remarkable change in the distribution of these three acid mucopolysaccharides during the aging process from 20 to 60 years or over.</p></span></li></ul></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90007-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15329922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 44
Isolation of neutral sugar-containing mucopolysaccharides from cattle retina 牛视网膜中含中性糖粘多糖的分离
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90015-1
E.R. Berman
{"title":"Isolation of neutral sugar-containing mucopolysaccharides from cattle retina","authors":"E.R. Berman","doi":"10.1016/0926-6534(65)90015-1","DOIUrl":"10.1016/0926-6534(65)90015-1","url":null,"abstract":"","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90015-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15329923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Chemical synthesis of N-acetylgalactosamine-αI-phosphate 6-O-sulphate, N-acetylchondrosine sulphate and UDP-N-acetylgalactosamine6-O-sulphate n -乙酰半乳糖胺-α i -磷酸6- o硫酸盐、n -乙酰硫酸软骨素和udp - n -乙酰半乳糖胺6- o硫酸盐的化学合成
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90001-1
A.H. Olavesen , E.A. Davidson
{"title":"Chemical synthesis of N-acetylgalactosamine-αI-phosphate 6-O-sulphate, N-acetylchondrosine sulphate and UDP-N-acetylgalactosamine6-O-sulphate","authors":"A.H. Olavesen ,&nbsp;E.A. Davidson","doi":"10.1016/0926-6534(65)90001-1","DOIUrl":"10.1016/0926-6534(65)90001-1","url":null,"abstract":"<div><p>The 6-<em>O</em>-sulphates of 2-acetamido-2-deoxy-α-<span>D</span>-galactose <span>I</span>-phosphate (<em>N</em>-acetylgalactosamine-<em>α</em><span>I</span>-phoshate), <span><math><mtext>O-(β-</mtext><mtext>D</mtext><mtext>-</mtext><mtext>glucopyranosyluronic acid</mtext><mtext>)-(</mtext><mtext>I</mtext><mtext>→3)-2-</mtext><mtext>acetamido</mtext><mtext>-2-</mtext><mtext>deoxy</mtext><mtext>-</mtext><mtext>D</mtext><mtext>-</mtext><mtext>galactase</mtext></math></span> (<em>N</em>-acetylchondrosine) and UDP-2-acetamido-2-deoxy-α-<span>D</span>-galactose (UDP-<em>N</em>-acetylgalactosamine) were synthesized using triethylamine-<em>N</em>-sulphonic acid as sulphating agent. The products of the reaction were purified by fractionation on ECTEOLA-cellulose and converted to the barium salts by passage through the barium form of a cation-exchange resin. Evidence for the homogeneity of the sulphates was provided by chemical analysis, paper chromatography, electrophoresis, colorimetric assay and infrared spectra. Proof for the position of the sulphate group on the primary hydroxyl of the <em>N</em>-acetylhexosamines was provided by periodate consumption studies and the detection of glycolaldehyde sulphate as an end product of periodate oxidation.</p></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90001-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15394418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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