{"title":"Composition of mucopeptide from the spores of Bacillus coagulans","authors":"A.D. Warth","doi":"10.1016/0926-6534(65)90010-2","DOIUrl":null,"url":null,"abstract":"<div><p>Spore mucopeptide was prepared by autolysis of the cortical structure of <em>Bacillus coagulans</em> spores. Analysis showed that it contained glucosamine, muramic acid, <span>L</span>-alanine, <span>D</span>-glutamic acid, <em>meso</em>-diaminopimelic acid and <span>D</span>-alanine in the molecular proportions 2.8:3:1.6:1.0:1.0:1.0. About 80% of the diaminopimelic acid residues had one amino group which reacted with fluorodinitrobenzene. Alanine in trace amounts was the only N-terminal amino acid detected. The presence of most of the expected free carboxyl and amino groups was confirmed by titration studies. Not all the amino sugar appeared to be N-acetylated. The results are consistent with the proposition that the spore cortical structure consists of a loosely cross-linked matrix bearing a considerable excess of carboxyl groups.</p></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90010-2","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926653465900102","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9
Abstract
Spore mucopeptide was prepared by autolysis of the cortical structure of Bacillus coagulans spores. Analysis showed that it contained glucosamine, muramic acid, L-alanine, D-glutamic acid, meso-diaminopimelic acid and D-alanine in the molecular proportions 2.8:3:1.6:1.0:1.0:1.0. About 80% of the diaminopimelic acid residues had one amino group which reacted with fluorodinitrobenzene. Alanine in trace amounts was the only N-terminal amino acid detected. The presence of most of the expected free carboxyl and amino groups was confirmed by titration studies. Not all the amino sugar appeared to be N-acetylated. The results are consistent with the proposition that the spore cortical structure consists of a loosely cross-linked matrix bearing a considerable excess of carboxyl groups.