Composition of mucopeptide from the spores of Bacillus coagulans

A.D. Warth
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引用次数: 9

Abstract

Spore mucopeptide was prepared by autolysis of the cortical structure of Bacillus coagulans spores. Analysis showed that it contained glucosamine, muramic acid, L-alanine, D-glutamic acid, meso-diaminopimelic acid and D-alanine in the molecular proportions 2.8:3:1.6:1.0:1.0:1.0. About 80% of the diaminopimelic acid residues had one amino group which reacted with fluorodinitrobenzene. Alanine in trace amounts was the only N-terminal amino acid detected. The presence of most of the expected free carboxyl and amino groups was confirmed by titration studies. Not all the amino sugar appeared to be N-acetylated. The results are consistent with the proposition that the spore cortical structure consists of a loosely cross-linked matrix bearing a considerable excess of carboxyl groups.

凝固芽孢杆菌孢子中粘肽的组成
通过对凝固芽孢杆菌孢子的皮层结构进行自溶制备孢子粘肽。分析表明,其含有氨基葡萄糖、氨基乙酸、l -丙氨酸、d -谷氨酸、中二氨基戊酸和d -丙氨酸,分子比例为2.8:3:1.6:1.0:1.0:1.0。约80%的二氨基亚苯甲酸残基有一个氨基与氟二硝基苯反应。微量丙氨酸是唯一检测到的n端氨基酸。大多数预期的游离羧基和氨基的存在被滴定研究证实。并不是所有的氨基糖都被n -乙酰化了。结果与孢子皮层结构由含有大量羧基的松散交联基质组成的命题一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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