牛巩膜的溶解与分离

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI:10.1016/0926-6534(65)90005-9

Jerome Rotstein M.D. , Jules Seltzer M.S.

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引用次数: 4

摘要

1.1. 描述了一种牛巩膜溶解和分馏的方法。高速均质，利用双水萃取，从巩膜中分离出蛋白多糖。剩余物用胃蛋白酶(EC 3.4.4.1)和胰蛋白酶(EC 3.4.4.4)溶解，用透析和冷醇沉淀法分离。硫酸软骨素A、B和C是在牛巩膜中发现的蛋白质-多糖复合物。3.3.组织中未见硫酸角蛋白。牛巩膜中不存在n -乙酰神经氨酸。牛巩膜和牛鼻软骨组织的比较表明，它们在组成和结构上是不同的。

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The dissolution and fractionation of bovine sclera

1.
1. A method for dissolving and fractionating bovine sclera has been described. High speed homogenization, utilizing double water extraction, separates out the protein-polysaccharide from the sclera. The residue is disolved with pepsin (EC 3.4.4.1) and trypsin (EC 3.4.4.4) and fractionated by dialysis and cold alcohol precipitation.
2.
2. Chondroitin sulfates A, B and C are found as protein-polysaccharide complexes in bovine sclera. No keratosulfate was found in the tissue.
3.
3. No N-acetyl neuraminic acid is present in bovine sclera.
4.
4. Comparison of the fractions of the bovine sclera and bovine nasal cartilage reveals that the tissues are different in composition and structure.

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Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides

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