Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides最新文献_第2页

The physical properties of a glycoprotein from bovine cortical bone (bone sialoprotein) 牛皮质骨糖蛋白(骨唾液蛋白)的物理性质

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90011-4

P.A. Williams, A.R. Peacocke

引用次数: 28

Studies on the structure of neuramin-lactose sulfate 神经胺-硫酸乳糖的结构研究

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90002-3

Leonard C. Ryan , Raul Carubelli , Ranwel Caputto , R.E. Trucco

{"title":"Studies on the structure of neuramin-lactose sulfate","authors":"Leonard C. Ryan , Raul Carubelli , Ranwel Caputto , R.E. Trucco","doi":"10.1016/0926-6534(65)90002-3","DOIUrl":"10.1016/0926-6534(65)90002-3","url":null,"abstract":"<div><ul><li>1.1. Chemical and enzymological studies on neuramin-lactose sulfate have provided supporting evidence for the proposed structure: <math><mtext>O-α-</mtext><mtext>D</mtext><mtext>-N-</mtext><mtext>acetylneuraminyl</mtext><mtext>-(2→3)-O-β-</mtext><mtext>D</mtext><mtext>-</mtext><mtext>galactopyranosyl</mtext></math><math><mtext>6-O-</mtext><mtext>sulfate</mtext><mtext>-(1→4)-</mtext><mtext>D</mtext><mtext>-</mtext><mtext>glucopyranose</mtext></math>.</li><li>2.2. The enzymological studies showed that neuramin-lactose sulfate is hydrolyzed by neuraminidase (EC 3.2.1.18) and that the lactose sulfate moiety of the molecule is resistant to the action of β-galactosidase (EC 3.2.1.23).</li><li>3.3. The chemical studies showed that during periodate oxidation of neuramin-lactose sulfate, the galactosyl moiety was protected, while in the case of lactose sulfate it underwent complete oxidation.</li><li>4.4. Synthetic D-galactose 6-O-sulfate and the galactose sulfate isolated from neuramin-lactose sulfate were found to be identical by paper chromatography, ionophoresis, infrared spectra and lack of formaldehyde production during periodate oxidation.</li></ul></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":"101 3","pages":"Pages 252-258"},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90002-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15394419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Further observations on the glycoproteins in human ovarian cyst fluids 人卵巢囊肿液中糖蛋白的进一步观察

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90009-6

J.R. Dunstone , W.T.J. Morgan

{"title":"Further observations on the glycoproteins in human ovarian cyst fluids","authors":"J.R. Dunstone , W.T.J. Morgan","doi":"10.1016/0926-6534(65)90009-6","DOIUrl":"10.1016/0926-6534(65)90009-6","url":null,"abstract":"<div>An examination has been made of a number of ovarian cyst fluid glycoproteins that are sparingly soluble water but are similar in general composition to the readily soluble blood-group specific glycloproteins obtained from the same source. Ultracentrifugal analysis of approx. 0.1% w/v solutions of the sparingly soluble materials indicate that they have much larger sedimentation coefficients (S20,w) and molecular weights than have the water-soluble glycoproteins. The sparingly soluble substances have more total amino acids and proportionally more aspartic and glutamic acids and cystine and less serine and threonine than have the soluble glycoproteins. The sparingly soluble substances mostly dissolve in buffered (pH 6.8–7.0, 0.05 M) solutions of thioglycolate, sulphite or cysteine, reagents that bring about the scission of -S-S-bonds. The behaviour and properties of the sparingly soluble glycoproteins suggest that they arise through the building up of relatively small glycoproteins to larger macromolecules through the formation of -S-S- intermolecular associations. A number of ways whereby the formation of these sparingly soluble glycoproteins could occur are discussed.</div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":"101 3","pages":"Pages 300-314"},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90009-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"16407509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Studies on the spore coats of Aspergillus oryzae 米曲霉孢子外壳的研究

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90014-X

Koki Horikoshi, Yonosuke Ikeda

引用次数: 17

The production of hyaluronate by spontaneously established cell lines and viral transformed lines of fibroblastic origin 由自发建立的细胞系和源自成纤维细胞的病毒转化细胞系产生的透明质酸

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90013-8

David Hamerman , George J. Todaro , Howard Green

引用次数: 96

Incorporation of D-[1-14C]galactosamine into serum proteins and tissues of the rat D-[1-14C]半乳糖胺在大鼠血清蛋白和组织中的掺入

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90003-5

Betty N. White , M.R. Shetlar , H.M. Shurley , J.A. Schilling

{"title":"Incorporation of D-[1-14C]galactosamine into serum proteins and tissues of the rat","authors":"Betty N. White , M.R. Shetlar , H.M. Shurley , J.A. Schilling","doi":"10.1016/0926-6534(65)90003-5","DOIUrl":"https://doi.org/10.1016/0926-6534(65)90003-5","url":null,"abstract":"<div>Incorporation of D-[1-14C]galactosamine into serum proteins and tissues of the rat was studied. After single intraperitoneal injections, about 20% of administered radioactivity was excreted in urine within 6 h. Only 1% was found in expired CO2 at this time; 12% was found at 72 h. High levels of bound (non-dialyzable) radioactivity appeared first in liver and then in serum. Non-dialyzable radioactivity in serum was found associated with all electrophoretically separated fractions with the greatest concentration in the α-globulins. Hexosamines were separated by paper chromatography from serum protein hydrolysates; glucosamine had a 14C content over ten times that of galactosamine in a sample taken 6 h after injection. A progressive increase in non-dialyzable radioactivity with time was found in both connective tissue and the fluid from within stainless-steel wire mesh cylinders implanted subcutaneously 2 weeks prior to injection of [1-14C]galactosamine. Mucopolysaccharides, extracted from connective tissue, exhibited electrophoretic mobility and staining characteristic of chondroitin sulfate and hyaluronic acid. Although hyaluronic acid and chondroitin sulfate moieties were labeled to the same extent, their hydrolysates contained glucosamine with 4 times more radioactivity then galactosamine, possibly indicating the presence of a substance containing glucosamine in the chondroitin sulfate area. These results indicate that much of the galactosamine is converted to glucosamine. Radioactive galactosamine appears to be of value for metabolic studies of glycoproteins and mucopolysaccharides.</div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":"101 3","pages":"Pages 259-266"},"PeriodicalIF":0.0,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90003-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91632741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Hydroxyproline-containing glycopeptides of some human and animal tissues 某些人和动物组织中含有羟脯氨酸的糖肽

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90008-4

S.D. Schultz-Haudt, J. Aarli, A. Lohrmann Nilsen, O. Unhjem

引用次数: 7

Separation of peptide components of urine of patients with thermal burns by column chromatography 热烧伤患者尿液中肽成分的柱层析分离

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90019-9

Frances L. Estes

引用次数: 6

Acid mucopolysaccharides of tadpole tail fin and back skin 蝌蚪尾鳍和背部皮肤的酸性粘多糖

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-11-01 DOI: 10.1016/0926-6534(65)90006-0

Marcia J. Lipson , Jeremiah E. Silbert

引用次数: 12

The polysaccharide sulphate-peptide complex in the hypobranchial mucin of Buccinum undatum L. 海底蛤鳃下黏液中硫酸多糖-肽复合物的研究。

Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-07-01 DOI: 10.1016/0926-6534(65)90052-7

S. Hunt, F.R. Jevons

引用次数: 9