Leonard C. Ryan , Raul Carubelli , Ranwel Caputto , R.E. Trucco
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引用次数: 17
摘要
1.1. 神经胺-乳糖硫酸酯的化学和酶学研究为该结构提供了支持证据:O-α- d - n-乙酰神经胺基-(2→3)-O-β- d -半乳糖氨基- 6-O-硫酸盐-(1→4)- d -葡萄糖吡喃糖。酶学研究表明，神经氨酸-乳糖硫酸酯可被神经氨酸酶(EC 3.2.1.18)水解，并且该分子的乳糖硫酸酯部分对β-半乳糖苷酶(EC 3.2.1.23)具有抗性。3.3。化学研究表明，神经胺-硫酸乳糖在高碘酸氧化过程中，半乳糖部分受到保护，而硫酸乳糖则完全氧化。通过纸层析、离子电泳、红外光谱和高碘酸盐氧化过程中不产生甲醛等方法，发现合成的d -半乳糖6- o -硫酸酯与从神经胺-硫酸乳糖分离得到的半乳糖硫酸酯完全相同。
Studies on the structure of neuramin-lactose sulfate
1.
1. Chemical and enzymological studies on neuramin-lactose sulfate have provided supporting evidence for the proposed structure: .
2.
2. The enzymological studies showed that neuramin-lactose sulfate is hydrolyzed by neuraminidase (EC 3.2.1.18) and that the lactose sulfate moiety of the molecule is resistant to the action of β-galactosidase (EC 3.2.1.23).
3.
3. The chemical studies showed that during periodate oxidation of neuramin-lactose sulfate, the galactosyl moiety was protected, while in the case of lactose sulfate it underwent complete oxidation.
4.
4. Synthetic D-galactose 6-O-sulfate and the galactose sulfate isolated from neuramin-lactose sulfate were found to be identical by paper chromatography, ionophoresis, infrared spectra and lack of formaldehyde production during periodate oxidation.
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