天然和改良血清口体样体的一些流体动力学性质

Anatoly Bezkorovainy
{"title":"天然和改良血清口体样体的一些流体动力学性质","authors":"Anatoly Bezkorovainy","doi":"10.1016/0926-6534(65)90012-6","DOIUrl":null,"url":null,"abstract":"<div><p>Human and bovine serum orosomucoids and their neuraminidase (EC 3.2.1.18) treated counterparts were characterized with respect to their sedimentation, diffusion, viscosity, and optical rotatory properties at pH 7 in cacodylate buffer. The human and bovine orosomucoids had molecular weights of 41600 and 37000 respectively by the sedimentation diffusion method. Calculations involving the frictional coefficient and viscosity increment indicated that the two orosomucoids were highly hydrated prolate shaped molecules with an axial ratio of 2.5. The removal of sialic acid caused a loss of the water of hydration with an increase of the axial ratio to 5 and no loss of helical structure.</p></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1965-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90012-6","citationCount":"16","resultStr":"{\"title\":\"Some hydrodynamic properties of native and modified serum orosomucoids\",\"authors\":\"Anatoly Bezkorovainy\",\"doi\":\"10.1016/0926-6534(65)90012-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Human and bovine serum orosomucoids and their neuraminidase (EC 3.2.1.18) treated counterparts were characterized with respect to their sedimentation, diffusion, viscosity, and optical rotatory properties at pH 7 in cacodylate buffer. The human and bovine orosomucoids had molecular weights of 41600 and 37000 respectively by the sedimentation diffusion method. Calculations involving the frictional coefficient and viscosity increment indicated that the two orosomucoids were highly hydrated prolate shaped molecules with an axial ratio of 2.5. The removal of sialic acid caused a loss of the water of hydration with an increase of the axial ratio to 5 and no loss of helical structure.</p></div>\",\"PeriodicalId\":100163,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1965-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6534(65)90012-6\",\"citationCount\":\"16\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926653465900126\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926653465900126","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 16

摘要

在pH为7的酸盐缓冲液中,研究了人类和牛血清类口体及其神经氨酸酶(EC 3.2.1.18)处理过的对应物的沉降、扩散、粘度和旋光性。经沉淀扩散法测定,人和牛的口体类分子质量分别为41600和37000。摩擦系数和黏度增量的计算表明,这两种orosomucoids是高度水合的长形分子,轴比为2.5。唾液酸的去除导致水化水的损失,轴比增加到5,而螺旋结构没有损失。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Some hydrodynamic properties of native and modified serum orosomucoids

Human and bovine serum orosomucoids and their neuraminidase (EC 3.2.1.18) treated counterparts were characterized with respect to their sedimentation, diffusion, viscosity, and optical rotatory properties at pH 7 in cacodylate buffer. The human and bovine orosomucoids had molecular weights of 41600 and 37000 respectively by the sedimentation diffusion method. Calculations involving the frictional coefficient and viscosity increment indicated that the two orosomucoids were highly hydrated prolate shaped molecules with an axial ratio of 2.5. The removal of sialic acid caused a loss of the water of hydration with an increase of the axial ratio to 5 and no loss of helical structure.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信