Isolation of fucose-rich glycopeptides from normal urine

A. Lundblad
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引用次数: 15

Abstract

The non-ultrafilterable material from pooled human urine was submitted to block electrophoresis and two fractions relatively rich in fucose but poor in other sugars and in proteins were further fractionated by various means. The material proved to contain a considerable number of fucose-rich glycopeptides, some of which were obtained in a reasonably pure state. The molecular weight was determined in three instances, giving values in the range of 5 000–10 000. Two of the glycopeptides were chemically characterized. One of them had a chemical composition very close to that given for purified blood group antigens. It was serologically inactive.

从正常尿液中分离富焦糖肽
从汇集的人尿中提取的非超滤物质进行阻滞电泳,并通过各种方法进一步分离出富集富集但其他糖和蛋白质含量较低的两个部分。该材料被证明含有相当数量的富焦糖肽,其中一些是在相当纯净的状态下获得的。在三种情况下确定了分子量,给出了5 000 - 10 000范围内的值。对其中两个糖肽进行了化学表征。其中一种抗原的化学成分与纯化的血型抗原非常接近。血清无活性。
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