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Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides最新文献

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Incorporation of radioactive glucosamine into submicrosomal fractions isolated from rat liver 从大鼠肝脏分离的亚微粒体中掺入放射性氨基葡萄糖
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90035-7
L. Helgeland
{"title":"Incorporation of radioactive glucosamine into submicrosomal fractions isolated from rat liver","authors":"L. Helgeland","doi":"10.1016/0926-6534(65)90035-7","DOIUrl":"10.1016/0926-6534(65)90035-7","url":null,"abstract":"<div><p>The incorporation of [1-<sup>14</sup>C[glucosamine into various submicrosomal fractions and cell sap from rat liver, as well as into plasmaproteins, has been examined after intraperitoneal injection. It has been shown that after 1 h there is a significant incorporation of protein-bound radioactivity into the submicrosomal fractions. 69% of the total radioactivity was found in the ultrasonic extract of the microsomal pellet, 28% in the subsequent deoxycholate extract and 3% associated with the ribosomes. Of the total radioactivity in the ultrasonic extract and the deoxycholate extract, more than 80% was found in the perchloric acid precipitate and only about 5–10% in the phosphotungstic acid precipitate. 15% of the protein-bound radioactivity in the perchloric acid precipitate of the ultrasonic extract was associated with a sialic acid fraction and about 1% found in the hexose fraction. Most of the remaining radioactivity was associated with glucosamine. The intracellular site for the biosynthesis of glycoproteins is discussed.</p></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90035-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23851668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 29
A glucose-containing glycopeptide from bovine aorta glycoprotein 从牛主动脉糖蛋白中提取的含葡萄糖的糖肽
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90040-0
B. Radhakrishnamurthy, A.F. Fishkin, G.S. Berenson
{"title":"A glucose-containing glycopeptide from bovine aorta glycoprotein","authors":"B. Radhakrishnamurthy,&nbsp;A.F. Fishkin,&nbsp;G.S. Berenson","doi":"10.1016/0926-6534(65)90040-0","DOIUrl":"10.1016/0926-6534(65)90040-0","url":null,"abstract":"","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90040-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23850056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Isolation of new polysaccharide sulphates from charonia lampas 新硫酸盐多糖的分离
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90026-6
Sadako Inoue
{"title":"Isolation of new polysaccharide sulphates from charonia lampas","authors":"Sadako Inoue","doi":"10.1016/0926-6534(65)90026-6","DOIUrl":"10.1016/0926-6534(65)90026-6","url":null,"abstract":"<div><p>A sulphated heteropolysaccharide named horatinsulphuric acid has been isolated from a sulphatase-rich gastropod, <em>Charonia lampas</em>. Horatinsulphuric acid, a mixture of analogous polymers, is obtained by the method of trypaflavin precipitation of sulphate esters. Fractionation made by column chromatography using DEAE-Sephadex separates the mixture into several fractions in which the sulphate content varies from 10 to 30%. The composition of the sugar portion consists of fucose, mannose, glucose, galactose, glucosarnine, galactosamine and sialic acid.</p></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90026-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23850060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
Extent of cross linkage in the murein sacculus∗ of Escherichia coli B cell wall 大肠杆菌B细胞壁小鼠小囊*的交联程度
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90038-2
I. Takebe
{"title":"Extent of cross linkage in the murein sacculus∗ of Escherichia coli B cell wall","authors":"I. Takebe","doi":"10.1016/0926-6534(65)90038-2","DOIUrl":"10.1016/0926-6534(65)90038-2","url":null,"abstract":"","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90038-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23850249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 48
Effect of prednisolone and hydrocortisone on distribution of mucopolysaccharides in the skin of rats 强的松和氢化可的松对大鼠皮肤粘多糖分布的影响
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90042-4
Sara Schiller , Nellie Blumenkrantz, Albert Dorfman
{"title":"Effect of prednisolone and hydrocortisone on distribution of mucopolysaccharides in the skin of rats","authors":"Sara Schiller ,&nbsp;Nellie Blumenkrantz,&nbsp;Albert Dorfman","doi":"10.1016/0926-6534(65)90042-4","DOIUrl":"10.1016/0926-6534(65)90042-4","url":null,"abstract":"","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90042-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23850058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
The chemistry of the cell walls of Bacillus anthracis: the effect of penicillin 炭疽芽孢杆菌细胞壁的化学性质:青霉素的作用
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90024-2
Ronald S. Ratney
{"title":"The chemistry of the cell walls of Bacillus anthracis: the effect of penicillin","authors":"Ronald S. Ratney","doi":"10.1016/0926-6534(65)90024-2","DOIUrl":"10.1016/0926-6534(65)90024-2","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Cell wall mucopeptide of <em>Bacillus anthracis</em>, strain V<sub>1</sub>B-189 was prepared from cells grown in the presence and absence of penicillin. Both types contain an unusual inner membrane.</p></span></li><li><span>2.</span><span><p>2. These were analysed for reducing sugars, amino sugars and amino acids. Both types contained glutamic acid, alanine and diaminopimelic acid as the principle amino acids in the mole ratios of 1:1.5:1.</p></span></li><li><span>3.</span><span><p>3. Penicillin did not change the relative amounts of the different cell wall constituents but it did reduce the weight of all constituents per unit weight of mucopeptide.</p></span></li></ul></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90024-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23850061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Conformation of the iduronic acid moiety of chondroitin sulfate B (Dermatan sulfate) 硫酸软骨素B (Dermatan sulfate)中伊杜醛酸部分的构象
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90037-0
E.A. Davidson
{"title":"Conformation of the iduronic acid moiety of chondroitin sulfate B (Dermatan sulfate)","authors":"E.A. Davidson","doi":"10.1016/0926-6534(65)90037-0","DOIUrl":"10.1016/0926-6534(65)90037-0","url":null,"abstract":"","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90037-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23850248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 20
On the metabolic role of β-glucuronidase β-葡萄糖醛酸酶的代谢作用
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90043-6
J. Mora, L. Can̄edo, G. Soberön
{"title":"On the metabolic role of β-glucuronidase","authors":"J. Mora,&nbsp;L. Can̄edo,&nbsp;G. Soberön","doi":"10.1016/0926-6534(65)90043-6","DOIUrl":"10.1016/0926-6534(65)90043-6","url":null,"abstract":"","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90043-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23850059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Investigations on the chemistry of heparin V. Disaccharides obtained after partial hydrolysis 肝素V.部分水解后二糖的化学性质研究
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90028-X
I. Danishefsky, Harvey Steiner
{"title":"Investigations on the chemistry of heparin V. Disaccharides obtained after partial hydrolysis","authors":"I. Danishefsky,&nbsp;Harvey Steiner","doi":"10.1016/0926-6534(65)90028-X","DOIUrl":"10.1016/0926-6534(65)90028-X","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The structure of the polysaccharide backbone of heparin was investigated by means of graded hydrolysis procedures.</p></span></li><li><span>2.</span><span><p>2. Desulfated acetamido heparin was partially hydrolyzed with acid and the products were fractionated on a cation exchange resin. This yielded glucosamine, disaccharides and a mixture of oligosaccharides.</p></span></li><li><span>3.</span><span><p>3. Two disaccharides were isolated as their acetamido derivatives. One of these was identified as <span><math><mtext>4-O-(2-</mtext><mtext>acetamido-2-deoxy-α-</mtext><mtext>d</mtext><mtext>-glucopyranoside)-</mtext><mtext>d</mtext><mtext>-glucopyranosyl-uronic acid</mtext></math></span> and the other as <span><math><mtext>4-O-(</mtext><mtext>d</mtext><mtext>-glucopyranosyluronic acid</mtext><mtext>)-2-</mtext><mtext>acetamido</mtext><mtext>-2-</mtext><mtext>deoxy-</mtext><mtext>d</mtext><mtext>-glucopyranose</mtext></math></span>.</p></span></li><li><span>4.</span><span><p>4. These results indicate that heparin contains alternating 1,4-linked units of glucosamine and glucuronic acid.</p></span></li></ul></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90028-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23850063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 55
The synthesis of teichoic acid IV. On the regulation of cytidine 5′-diphosphateglycerol concentration 磷壁酸的合成。胞苷5′-二磷酸甘油酯浓度的调控
Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides Pub Date : 1965-03-01 DOI: 10.1016/0926-6534(65)90025-4
Luis Glaser
{"title":"The synthesis of teichoic acid IV. On the regulation of cytidine 5′-diphosphateglycerol concentration","authors":"Luis Glaser","doi":"10.1016/0926-6534(65)90025-4","DOIUrl":"10.1016/0926-6534(65)90025-4","url":null,"abstract":"<div><p>The partial purification of a cytidine 5′-diphosphateglycerol pyrophosphatase from <em>Bacillus subtilis</em> (NCTC 3610) is described. The <em>K</em><sub><em>m</em></sub> for cytidine 5′-diphosphateglycerol is 1.6·10<sup>−3</sup><em>M</em>. It is suggested that the function of this enzyme is to prevent the accumulation of cytidine 5′-diphosphateglycerol under conditions where the rate of teichoic acid synthesis is decreased. A similar mechanism is suggested for the control <em>in vivo</em> of the concentration of some other nucleoside diphosphate sugars.</p></div>","PeriodicalId":100163,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Mucoproteins and Mucopolysaccharides","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1965-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6534(65)90025-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23850067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
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