Investigations on the chemistry of heparin V. Disaccharides obtained after partial hydrolysis

I. Danishefsky, Harvey Steiner
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引用次数: 55

Abstract

  • 1.

    1. The structure of the polysaccharide backbone of heparin was investigated by means of graded hydrolysis procedures.

  • 2.

    2. Desulfated acetamido heparin was partially hydrolyzed with acid and the products were fractionated on a cation exchange resin. This yielded glucosamine, disaccharides and a mixture of oligosaccharides.

  • 3.

    3. Two disaccharides were isolated as their acetamido derivatives. One of these was identified as 4-O-(2-acetamido-2-deoxy-α-d-glucopyranoside)-d-glucopyranosyl-uronic acid and the other as 4-O-(d-glucopyranosyluronic acid)-2-acetamido-2-deoxy-d-glucopyranose.

  • 4.

    4. These results indicate that heparin contains alternating 1,4-linked units of glucosamine and glucuronic acid.

肝素V.部分水解后二糖的化学性质研究
1.1. 采用分级水解法对肝素多糖骨架结构进行了研究。用酸对脱硫后的乙酰氨基肝素进行部分水解,产物在阳离子交换树脂上进行分馏。这就产生了葡萄糖胺、双糖和低聚糖的混合物。分离得到两个双糖作为它们的乙酰氨基衍生物。其中一个鉴定为4-O-(2-乙酰氨基-2-脱氧-α-d-葡萄糖吡喃苷)-d-葡萄糖吡喃醛酸,另一个鉴定为4-O-(d-葡萄糖吡喃醛酸)-2-乙酰氨基-2-脱氧-d-葡萄糖吡喃糖。这些结果表明肝素含有葡萄糖胺和葡萄糖醛酸交替的1,4-连接单元。
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