Animal Research and One Health最新文献

{"title":"Multi-omics analyses reveal the interaction between colonic microbiota and host in Min and Yorkshire pigs","authors":"Xiaoyu Huang,&nbsp;Huihui Li,&nbsp;Feng Cheng,&nbsp;Ligang Wang,&nbsp;Guoqing Cao,&nbsp;Lixian Wang,&nbsp;Lijun Shi","doi":"10.1002/aro2.39","DOIUrl":"10.1002/aro2.39","url":null,"abstract":"<p>Adoption of microbial preparations is becoming more and more prevalent in the pig breeding industry. Digestive tract microbes are recognized as crucial elements affecting physical characteristics of pigs. Until now, it is still challenging to establish connections between colonic microbiome and the host. In this study, weight gain models were created for Min and Yorkshire pigs. The colonic contents and colonic tissues were collected from two pig purebred strains (<i>n</i> = 8/group) with similar weights for multi-omics analysis. By difference analysis of colonic microbiota, Min pigs observed a significantly higher relative abundance of <i>Bacteroides</i>, <i>Phocaeicola</i>, <i>Roseburia</i>, and <i>Parabacteroides</i>, and Yorkshire pigs had a strongly higher relative abundance of <i>Streptococcus</i>, <i>Vescimonas</i>, <i>Ligilactobacillus</i>, and <i>Lactococcus</i>. Functional annotation showed that the colonic microbiota of Min pigs had extensive dietary polysaccharide and immunomodulatory capacity. Through the correlation analysis of colonic microbiota with metabolomics of colonic content or transcriptomics of colonic tissues, we provided direct and indirect relationships of microorganisms and hosts. By verification, the contents of Occludin, ZO-1, and pIgR in colonic tissue and sIgA, sIgG, and sIgM in colonic contents of Min pigs were significantly higher than that in Yorkshire pigs. This study revealed characteristics and functions of the colonic microbiota in Min and Yorkshire pigs and analyzed their interactions with the host. Also, we identified immune-related microorganisms. These results provided a theoretical basis for understanding the influence of colonic microbiota on phenotype shaping in pigs.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"3 3","pages":"278-296"},"PeriodicalIF":0.0,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.39","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138595847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative analysis of genome-wide association study and transcriptomics to identify potential candidate genes influencing drip loss in Beijing Black pigs","authors":"Hongmei Gao,&nbsp;Jingjing Tian,&nbsp;Run Zhang,&nbsp;Xiance Liu,&nbsp;Hai Liu,&nbsp;Fuping Zhao,&nbsp;Zhenhua Xue,&nbsp;Lixian Wang,&nbsp;Xitao Jing,&nbsp;Longchao Zhang","doi":"10.1002/aro2.32","DOIUrl":"https://doi.org/10.1002/aro2.32","url":null,"abstract":"<p>Understanding the genetic factors related to meat drip loss is of great importance for animal breeding and production. In this study, we employed a combination of genome-wide association study (GWAS) mapping and RNA sequencing (RNA-seq) data to effectively identify potentially functional single nucleotide polymorphisms (SNPs) as well as candidate genes associated with drip loss (DL) in Beijing Black pigs. Initially, we conducted a single- and multi-trait GWAS on drip loss traits in 441 Beijing Black pigs at 24 (DL24) and 48 (DL48) hours postmortem using the Illumina pig 50K SNP chip. Five SNPs with annotations for four genes (<i>FGGY</i>, <i>LHFPL6</i>, <i>OSBPL1A</i>, and <i>NMNAT3</i>) were consistently identified in single or multiple trait GWAS results, indicating their potential pleiotropic effects on drip loss. Next, a comprehensive comparative transcriptomic analysis was performed on samples of Beijing Black pigs exhibiting extremely high and low drip loss, resulting in the identification of 21 differentially expressed genes (DGEs) as potential candidates. Additionally, protein–protein interaction (PPI) network analysis revealed reciprocal regulatory relationships between <i>FOXO1</i>, <i>OSBPL1A</i>, <i>DOCK1</i> (identified from GWAS) and the candidate DGEs obtained from RNA-seq data. Therefore, we propose that these genes may impact drip loss traits through gene interactions. In conclusion, our integrative analysis screened candidate genes that may affect the drip loss traits in Beijing Black pigs, which provides crucial insights into the molecular mechanisms of drip loss and serves as a theoretical reference for improving meat quality in Beijing Black pigs.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 4","pages":"446-457"},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.32","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective analysis of resistance and susceptibility to duck hepatitis A virus genotype 3 in Pekin duck","authors":"Suyun Liang,&nbsp;Jiaojiao Zhang,&nbsp;Xiaoyan Wang,&nbsp;Guangnan Xing,&nbsp;Zhanbao Guo,&nbsp;Qi Zhang,&nbsp;Wei Huang,&nbsp;Ming Xie,&nbsp;Shuisheng Hou","doi":"10.1002/aro2.36","DOIUrl":"https://doi.org/10.1002/aro2.36","url":null,"abstract":"<p>Duck viral hepatitis (DVH), mainly caused by duck hepatitis A virus genotype 3 (DHAV-3) in China, is an important disease affecting Pekin ducks. Using artificial selection breeding based on genealogical and phenotypic observations, a susceptible line (Z7) and a resistant line (Z8) of Pekin ducks to DHAV-3 were identified. Here, we performed a genome-wide analysis to identify selected genes in the genomes of Pekin ducks underlying resistance/susceptible breeding. Following selection, the mortality rate of the Z8 line reduced from 59.2% to 7.8% in the fourth generation (Z8G4), whereas the death rate of the Z7 line increased from 67.5% to 81% in the third generation (Z7G3). Moreover, directed breeding caused the allele frequencies of Z8 and Z7 changing in opposite direction, accompanied by declines in genomic genetic diversity. With the G0 generation as the reference group, a total of 49 selected genes were identified in the Z7-susceptible population and 109 selected genes in the Z8-resistant population based on the top 5% <i>F</i>_ST and PI ratio, and two candidate key genes were further fine-mapped. Susceptibility selection led to 17 mutations in the <i>LRIG3</i> gene in the Z7 population (chr1: 169,757,982–169,772,687), and resistance selection led to 134 mutations in the <i>CRHR2</i> gene in the Z8 population (chr2: 4,190,154–4,273,970). Our results provide new insights into the resistance and susceptibility to DHAV-3 and lay a theoretical foundation for further research on the mechanism of resistance/susceptibility of Pekin ducks to DHAV-3.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"1 2","pages":"146-155"},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.36","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138449598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The establishment and application of a one-step multiplex real-time polymerase chain reaction assay for the detection of Streptococcus suis, Streptococcus suis serotype 2, and Glaesserella parasuis","authors":"Lingxiang Xin,&nbsp;Haojie Wang,&nbsp;Yunhao Hu,&nbsp;Yan Liu,&nbsp;Wensheng Yao,&nbsp;Xiuli Wang,&nbsp;Jian Li,&nbsp;Yuanjie Liu,&nbsp;Rendong Tong,&nbsp;Qi Wang,&nbsp;Youlong Lu,&nbsp;Liangquan Zhu","doi":"10.1002/aro2.37","DOIUrl":"https://doi.org/10.1002/aro2.37","url":null,"abstract":"<p>This article aims to establish a multiplex real-time polymerase chain reaction (PCR) assay for the simultaneous detection of <i>Streptococcus suis</i> (SS), <i>Streptococcus suis</i> serotype 2 (SS2), and <i>Glaesserella parasuis</i> (GPS). In this study, three pairs of primers and three probes were designed based on the specific sequences of SS (<i>gdh</i>), SS2 (<i>cps2j</i>), and GPS (<i>infB</i>). The results showed that the assay was not cross-reacted with other swine pathogens (<i>Escherichia coli</i>, <i>Pasteurella multocida</i>, <i>Staphylococcus aureus</i>, <i>Streptococcus agalactiae</i>, <i>Streptococcus pneumoniae</i>, <i>Actinobacillus pleuropneumoniae</i>, <i>Mycoplasma hyopneumoniae</i>, and <i>Enterococcus faecalis; Streptococcus pyogenes</i>). 10⁸ to 10² copies/μL showed the <i>R</i>² values for SS, SS2, and GPS were 0.999, 0.992, and 0.990, respectively. The multiplex real-time PCR efficiency was 93.816% for <i>gdh</i>, 105.260% for <i>cps2j</i>, and 93.175% for <i>infB</i>. The sensitivity result showed that SS, SS2, and GPS could be detected at 10 copies/μL. The repeatability result showed that intra-assay and inter-assay coefficients of variation of SS, SS2, and GPS were &lt;2%. The best cutoff values for SS, SS2, and GPS were determined from ROC curves to be 35.085, 35.620, and 34.940, respectively. Areas under the curve were 0.943, 0.968, and 0.958. In total, 88 clinical samples were analyzed. The results indicated positive rates of 11.364% (10/88) for SS, 20.455% (18/88) for SS2, and 18.182% (16/88) for GPS. In conclusion, the developed one-step multiplex real-time PCR assay may be a valuable tool for the early detection of the SS, SS2 and, GPS with high specificity and sensitivity.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 1","pages":"59-70"},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.37","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139745068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted metabolomics investigating porcine reproductive and respiratory syndrome virus biomarkers of serum-derived exosomes in piglets infected with PRRSV NADC30-like CHsx1401","authors":"Feng Cheng,&nbsp;Jia Li,&nbsp;Chunxiang Zhou,&nbsp;Lei Zhou,&nbsp;Ganqiu Lan,&nbsp;Jing Liang,&nbsp;Lixian Wang,&nbsp;Ligang Wang","doi":"10.1002/aro2.34","DOIUrl":"https://doi.org/10.1002/aro2.34","url":null,"abstract":"<p>Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most severe swine diseases in the pig industry. The identification of biomarkers for PRRSV infection is valuable for controlling, eliminating, and treating PRRSV. This study utilized the ultra-performance liquid chromatography–mass spectrometry metabolite profiling platform to identify differential metabolites in exosomes between the control and NADC30-like PRRSV strain infected pigs. Using multivariate analysis combined with univariate analysis, unsupervised principal component analysis and orthogonal partial least squares discriminant analysis models were constructed between the groups. A total of 41 differential metabolites were detected, with 14 upregulated and 27 downregulated metabolites with PRRSV infection. MetaboAnalyst and Kyoto Encyclopedia of Genes and Genomes were used to identify potentially relevant significant pathways, and a receiver operating characteristic curve was used to quantify the predictive performance of differential metabolites. The results indicated that tryptophan-related L-kynurenine, 5-hydroxytryptophan, and D (+)-tryptophan significantly increased among PRRSV infected groups, which may play an important role in the progression of PRRSV infection. Metabolites related to amino acid synthesis and metabolism, including 2-arachidonoylglycerol Lysopcs and phosphatidylcholines may also contribute to the lack of immune protection in piglets after PRRSV infection. Moreover, L-kynurenine and taurocholic acid may serve as potential biomarkers for early diagnosis or drug targeting of PRRSV. Overall, these findings provide an important reference to our understanding of PRRS pathogenesis and immune or protective responses during PRRSV acute infection in the host.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"3 1","pages":"102-113"},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.34","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143489845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why “One Health” and animal welfare is key to sustainability for people and the global ecosystem","authors":"Philip Lymbery","doi":"10.1002/aro2.40","DOIUrl":"https://doi.org/10.1002/aro2.40","url":null,"abstract":"<p>I was taking the night train to Nanyang in China's Henan province and spent the last hour before I reached my destination looking at mile upon mile of maize, or corn as it’s often known. Most of this crop in China and worldwide is grown largely for animal feed and biofuel. The air was dampened by a gray, misty haze. I was keen to visit some of China's animal production facilities but for now I found myself looking out over what was being used to feed them. Vast prairie-like tracts of a single crop stretching as far as the eye could see. So much so, that it made it feel like the train was moving in slow motion.</p><p>Thinking back on that journey reminds me of something that happened some years later much closer to home.</p><p>It was early morning in a field near the farm hamlet where I live in England, and a tractor was pulling a plough. Back and forth it went, ploughing its lonely furrow. Behind the tractor, dust clouds spiraled and caught the sun, creating an aura. A timeless symbol of the season. Only, something was missing: there were no screeching gulls following the plough in search of worms.</p><p>I took a closer look. The tractor was ploughing across a footpath, giving me a bird's-eye view of the newly upturned soil. As I stared down, do you know what I saw?—nothing. There were no worms, beetles, or bugs desperate to get back into the newly upturned earth. The soil was lifeless. It was like sand. We could have been walking on the moon.</p><p>That field should have had millions of worms in every hectare—in every patch the size of a football pitch. There should have been 13,000 species of life with a collective weight of an elephant: five tons.</p><p>But instead, there was nothing.</p><p>That field was about to be planted again with maize (corn), a crop commonly used as animal feed. It was grown with chemical pesticides and artificial fertilizers. No wonder the soil was dead and washing into the nearby river. It is a problem that exists for crops grown using industrial methods, whether for human consumption or the sizeable industry for animal feed, which accounts for about a third of cereal crops grown worldwide.</p><p>It reminded me of seeing great green swathes of monoculture maize corn in the American Midwest of Nebraska, much of which was destined for the feed troughs of industrially reared chickens, pigs, and cattle. I remember seeing feedlots. Hundreds of cows and calves standing in barren pens, not a blade of grass in sight. Despite the hot summer sun, they had no shade. I watched as they jostled in the searing heat for respite, trying to get into each other's shadow.</p><p>It was a potent example of industrial animal agriculture, a regime that now ravages the planet, to the detriment of animal welfare, the ecosystem, and the health of people.</p><p>It has not always been like this. In fact, it was but a single human lifetime ago when we started removing animals from the land to be caged, crammed, and confined. Taken off grass whe","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"1 2","pages":"259-263"},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.40","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138449494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration analysis of transcriptome and proteome of Chinese Merino sheep (Ovis aries) embryonic skeletal muscle","authors":"Mian Feng,&nbsp;Wenping Hu,&nbsp;Xinyue Wang,&nbsp;Lulu Liu,&nbsp;Yunhui Liu,&nbsp;Li Zhang","doi":"10.1002/aro2.33","DOIUrl":"10.1002/aro2.33","url":null,"abstract":"<p>The growth and development of sheep late fetal skeletal muscle undergoes significant changes. However, the specific mechanism remains unknown. In this study, we performed the comprehensive analysis of transcriptome and proteome of Chinese Merino sheep at embryonic ages Day85 (D85N), Day105 (D105N), and Day135 (D135N) by the tandem mass tags (TMT) and RNA-seq methods. Totally 717, 1253, and 1873 differentially expressed genes (DEGs) were identified in the three comparison groups (D85N vs. D105N, D105N vs. D135N, and D85N vs. D135N). Among which 7, 80, and 162 DEGs were identified with the same trends at mRNA and protein levels in the three groups. Enrichment analysis showed that 7 genes with same trends in D85 vs. D105 have not been enriched in any pathways, which indicated that the development of skeletal muscle underwent significant changes with post-transcription regulation during this period. These genes with same trends in D105N vs. D135N were mainly enriched in the pathways related to skeletal muscle metabolism and maturation, including oxidative phosphorylation, glycolysis/gluconeogenesis, tight junction, and HIF-1 pathways, which indicated that the development of skeletal muscle tended to maturation during this period. These results provided evidence for ovine late fetal skeletal muscle fibers development from proliferating to thickening at simultaneous transcriptional and translational levels.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"3 1","pages":"129-142"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.33","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135270619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing and evaluating a cost-effective single nucleotide polymorphism liquid array for Chinese native chickens","authors":"Yifan Liu,&nbsp;Yanju Shan,&nbsp;Yunjie Tu,&nbsp;Ming Zhang,&nbsp;Gaige Ji,&nbsp;Xiaojun Ju,&nbsp;Shiying Shi,&nbsp;Chenyu Fan,&nbsp;Yunlei Li,&nbsp;Jingting Shu","doi":"10.1002/aro2.31","DOIUrl":"10.1002/aro2.31","url":null,"abstract":"<p>Genotyping plays an important role in breeding and population studies. Currently available genotyping technologies, including solid-phase chips and sequencing, often have several limitations in their application to local chickens in China, including high costs, insufficient diversity, and poor universality. In the present study, we developed a chicken 5 K single nucleotide polymorphism (SNP) array suitable for breeding and genetic analysis using genotyping by targeted sequencing technology. The chip design was based on genomic data from 33 local breeds, and 5847 SNPs were selected for the final chip design. Among these SNPs, 3427 sites were associated with economic traits in broiler chickens. Our chip contained 25,000 high-quality SNP markers captured from 5 K regions with highly efficient target site capture. Population analyses of the eight breeds showed high detection rates and minor allele frequencies for SNP markers on the array, enabling clear differentiation of different populations and families within populations. The genetic diversity trend obtained using the chip was consistent with the results obtained from microsatellite analyses. Additionally, the 5 K array was applied to a genome-wide association study of broilers, resulting in the identification of several growth- and meat quality-related loci. Therefore, the newly developed chip is efficient, cost-effective, and well suited for application in local Chinese chickens, which will accelerate poultry breeding improvements and enhance conservation efforts.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"1 2","pages":"168-179"},"PeriodicalIF":0.0,"publicationDate":"2023-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.31","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135804349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of multiomics data identifies candidate genes influencing pH levels in Beijing Black pigs","authors":"Jingjing Tian,&nbsp;Naiqi Niu,&nbsp;Xiaoqing Wang,&nbsp;Liangyu Shi,&nbsp;Liyu Yang,&nbsp;Mianyan Li,&nbsp;Lijun Shi,&nbsp;Xin Liu,&nbsp;Hongmei Gao,&nbsp;Xinhua Hou,&nbsp;Ligang Wang,&nbsp;Lixian Wang,&nbsp;Longchao Zhang,&nbsp;Fuping Zhao","doi":"10.1002/aro2.26","DOIUrl":"10.1002/aro2.26","url":null,"abstract":"<p>pH value is a crucial index used to evaluate pork quality due to its direct impact on specific meat characteristics. This study investigated the genetic mechanisms influencing pH values through measurements taken from the longissimus dorsi muscle of Beijing Black pigs at 2 h (pH_2h) and 24 h (pH_24h) postmortem. A total of 614 Beijing Black pigs were subsequently genotyped using the Illumina Porcine 50K SNP Chip. Heritability estimates for pH_2h and pH_24h were found to be 0.19 and 0.25, respectively, with a genetic correlation of 0.53. Furthermore, we conducted both a genome-wide association study (GWAS) and an RNA sequencing (RNA-seq) analysis, the latter of which identified differentially expressed genes (DEGs) between high and low pH groups. We identified 31, 6, and 32 single-nucleotide polymorphisms in the pH_2h, pH_24h, and pH_2–24h traits, respectively. The GWAS results revealed the presence of the <i>SYT5</i> gene in both the pH_2h and pH_2–24h traits, while the <i>SNX13</i> gene was simultaneously identified in the pH_24h and pH_2–24h traits. The RNA-seq results also found <i>SYT5</i> to be highly expressed, while <i>SNX13</i> did not exhibit differential expression. Moreover, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses based on the DEGs revealed potential links between pH levels and the glycogen metabolic process as well as associations with the regulation of cell proliferation and calcium ion transmembrane transport. Ultimately, <i>SYT5</i> and <i>SNX13</i> emerged as key candidate genes affecting pH values at 2 and 24 h, respectively. These findings contribute to a better understanding of the genetic mechanisms affecting pork quality and safety and offer insights for enhancing meat quality through genetic improvement.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 3","pages":"260-272"},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.26","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136359965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy metal levels in the dairy production chain in China","authors":"Shengsheng Liu,&nbsp;Qingyu Zhao,&nbsp;Chaohua Tang,&nbsp;Yuning Liu,&nbsp;Junmin Zhang","doi":"10.1002/aro2.30","DOIUrl":"10.1002/aro2.30","url":null,"abstract":"<p>The aims of this study were to investigate the heavy metal pollution status and distribution and to analyze the relationships among metal concentrations of different links in the dairy production chain. Chromium (Cr), arsenic (As), cadmium (Cd), lead (Pb), copper (Cu), and zinc (Zn) levels in water, fodder, milk, blood, hair, and feces samples collected from cows from five dairy farms in China were measured. The concentrations of Cr, As, Cd, Pb, Cu, and Zn were 4.61–11.11, 0.06–0.46, 0.17–0.29, 2.84–4.23, 4.11–7.72, and 7.23–19.91 mg/kg in silage; 12.72–40.85, 0.76–2.40, 0.05–0.16, 4.73–9.16, 44.14–78.46, and 4148.51–4845.20 μg/L in milk; 33.59–60.73, 0.09–3.57, 0.29–1.78, 20.14–39.20, 821.34–1007.45, and 2665.10–4929.95 μg/L in blood; 0.59–1.06, 0.02–0.09, 0.08–0.11, 1.70–2.09, 6.45–8.64, and 9.73–1.56 mg/kg in hair; 31.75–296.35, 0.33–5.96, 0.18–0.53, 3.35–9.06, 12.27–41.39, and 56.54–196.34 mg/kg in feces, respectively. The concentrations of heavy metals in hair were higher than those in the other samples and when combined with the results from the silage and tissue samples, we can assume that cow's hair can be used as a biological indicator for heavy metal contamination in the dairy production chain.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 3","pages":"300-307"},"PeriodicalIF":0.0,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.30","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135900087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}