Animal Research and One Health最新文献

{"title":"Animal biotech breeding and reproduction: A new engine for high-quality development of animal husbandry","authors":"Yong Zhang, Jun Liu","doi":"10.1002/aro2.84","DOIUrl":"https://doi.org/10.1002/aro2.84","url":null,"abstract":"<p>Animal-based products, such as meat, eggs, milk, and their by-products, serve as the predominant protein sources for humans and are vital for supporting physiological functions. Animal proteins align more closely with our nutritional requirements compared to plant-based proteins. With the ever-growing global population, the demand for these fundamental food sources is increasing. Globally, about 97.64 million tons of livestock products, including meat, eggs, and milk, are consumed annually, accounting for nearly 48% of all feed grain usage. Nevertheless, the livestock industry imposes a substantial environmental footprint, contributing to 57% of the total CO<sub>2</sub> emissions from food production. Amidst escalating resource limitations and evolving geopolitical dynamics, the security of our livestock food supply is in threat. To address these challenges, the livestock sector must prioritize high-quality growth through innovative scientific and technological breakthroughs.</p><p>Exceptional livestock and poultry breeds contribute over 40% to the advancement of the livestock industry and are instrumental in enhancing its productivity. The integration of biotechnology, information technology, and artificial intelligence is driving animal breeding into a more efficient and precise phase known as Animal Breeding 4.0. A new wave of high-efficiency breeding technologies, exemplified by genomic selection (GS), gene editing, and in vitro embryo production accelerates targeted animal breeding significantly by enhancing breeding efficiency and reducing the breeding cycle time. GS has been widely adopted for cattle, pig, and sheep breeding, cutting breeding costs by 90% and boosting the genetic progress of critical traits by 50%. The industrialization of genetically modified animals is gaining momentum and promises to offer competitive advantages over conventional breeding methods. Currently, several types of gene-edited animals have passed safety evaluations, including GalSafe, a gene-knockout pig approved by the U.S. FDA in 2020, gene-edited heat stress-resistant beef cattle approved by the U.S. FDA in 2022, and gene-edited tiger puffer-fish and red snapper approved by the Japanese Ministry of Health, Labor, and Welfare in 2021. With the progression of multi-omics technology, key trait-related functional genes in livestock and poultry are continually being uncovered. The application of novel biological breeding techniques is anticipated to generate more diverse livestock and poultry varieties with high-yield, high-quality, disease-resistant, and environmentally friendly phenotypes.</p><p>This special issue, entitled <i>Animal Biotech Breeding and Reproduction</i>, is designed to facilitate academic dialog in this special domain, fostering the convergence of significant insights, breakthroughs, technological advancements, and industrial growth in the areas of molecular breeding and reproduction. The scope is to accelerate the pace of scientific and t","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 4","pages":"354-355"},"PeriodicalIF":0.0,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.84","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Duck 1000 Genomes Project: Achievements and perspectives","authors":"Wenlei Fan,&nbsp;Shuisheng Hou,&nbsp;Zhengkui Zhou","doi":"10.1002/aro2.89","DOIUrl":"https://doi.org/10.1002/aro2.89","url":null,"abstract":"<p>The duck (<i>Anas platyrhynchos</i>) is not only a vital farm animal but also an excellent model for genetic dissection of economic traits. The integration of multiomics data provides a powerful approach to elucidate the genetic basis of domestication and phenotype variation. Since its inception in 2014, the Duck 1000 Genomes Project has aimed to uncover the genetic foundation of key economic traits in ducks by combining multiomics data including genomic, transcriptomic, and metabolomic from various natural and segregating populations. This paper summarizes the strategies and achievements of the Duck 1000 Genomes Project, highlighting the reference genome assembly, genome evolution analysis, and the identification of genes and causative mutations responsible for key economic traits in ducks. We also discuss perspectives and potential challenges in functional genomic studies that could further accelerate duck molecular breeding.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 4","pages":"366-376"},"PeriodicalIF":0.0,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.89","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142664987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The paradigm of genomic selection: Does it need an update?","authors":"Johannes A. Lenstra","doi":"10.1002/aro2.88","DOIUrl":"https://doi.org/10.1002/aro2.88","url":null,"abstract":"&lt;p&gt;The genetics and genomics of livestock is, as for other species, a dynamic and successful field of research. It is divided into two clearly different, although closely interacting disciplines: the molecular and the quantitative genetics. Remarkably, this contrast has a close parallel in the opposing views during a short and fierce war (1904–1906) between Mendelians and biometricians. Although the accepted views soon became more balanced [&lt;span&gt;1, 2&lt;/span&gt;], the 20th century saw the emergence of two distinct genetic disciplines.&lt;/p&gt;&lt;p&gt;The development of the molecular genetics is an amazing and unending series of pioneering success stories featuring a legion of Nobel prize winners [&lt;span&gt;3&lt;/span&gt;]: from chromosomes to DNA and to the central dogma; from recombinant DNA to PCR, microsatellites and SNPs; the routine whole-genome sequencing (WGS) with telomere to telomere genomes and pangenomes as the newest toys; and now also the CRISPR/Cas9 gene editing, although not yet of primary relevance for livestock [&lt;span&gt;4, 5&lt;/span&gt;]. This was all typical laboratory science, which now has become a lot cleaner by automation and a growing emphasis on bioinformatics.&lt;/p&gt;&lt;p&gt;It illustrates the hectic progress that the promises made after one breakthrough were fulfilled after the next. Southern blotting of restriction fragment length polymorphism (RFLP) markers in the 80s and a little later the PCR–RFLP did not deliver the intended dense genetic map of a genome, so the discovery at the end of the decade of the microsatellites was most timely. This allowed the genetic mapping of monogenic traits, but until 20 years ago most causative mutations in livestock species were found via the candidate gene approach [&lt;span&gt;1, 6&lt;/span&gt;]. In the new millennium microsatellites were replaced by high-density genome-wide SNP arrays, which deliver accurate genetic localizations. At the same time, WGS became affordable and monogenic causative variants became sitting ducks. However, we did not unravel the molecular mechanisms of complex traits [&lt;span&gt;6, 7&lt;/span&gt;], so now we accept a less than satisfactory infinitesimal model of countless small contributions [&lt;span&gt;4&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;Starting during the decade of WWII, the quantitative geneticists, who never touch a pipette, started to provide scientific support to the breeding industry and developed the concept of breeding values [&lt;span&gt;8&lt;/span&gt;]. For a long time, this was solely based on phenotypes, but they did not hesitate to exploit the advances in the molecular field. During the last 2 decades of the millennium the concept or dream of master-assisted selection was an important source of inspiration [&lt;span&gt;9, 10&lt;/span&gt;]. This led to genetic localizations of enough quantitative trait loci (QTL) to fill the Animal QTLdb, but these explain only a small part of the phenotypic variation [&lt;span&gt;4&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;Again, we needed another breakthrough to fulfill the promises already made. In a visionary paper, Meuwissen et al. proposed ge","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 4","pages":"360-362"},"PeriodicalIF":0.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.88","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142664628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association study and genomic prediction for growth traits in spotted sea bass (Lateolabrax maculatus) using insertion and deletion markers","authors":"Chong Zhang,&nbsp;Yonghang Zhang,&nbsp;Cong Liu,&nbsp;Lingyu Wang,&nbsp;Yani Dong,&nbsp;Donglei Sun,&nbsp;Haishen Wen,&nbsp;Kaiqiang Zhang,&nbsp;Xin Qi,&nbsp;Yun Li","doi":"10.1002/aro2.87","DOIUrl":"https://doi.org/10.1002/aro2.87","url":null,"abstract":"<p>Spotted sea bass (<i>Lateolabrax maculatus</i>) is a species of significant economic importance in aquaculture. However, genetic degeneration, such as declining growth performance, has severely impeded industry development, necessitating urgent genetic improvement. Here, we conducted a genome-wide association study (GWAS) and genomic prediction for growth traits using insertion and deletion (InDel) markers, and systematically compared the results with our previous studies using single nucleotide polymorphism (SNP) markers. A total of 97 significant InDels including a 6 bp insertion in an exon region were identified. It is worth noting that only 5 and 1 candidate genes for DY and TS populations were also detected in previous GWAS using SNPs, and numerous novel genes including <i>c4b</i>, <i>fgf4</i>, and <i>dnajb9</i> were identified as vital candidate genes. Moreover, several novel growth-related procedures, such as the growth and development of the bone and muscle, were also detected. These findings indicated that InDel-based GWAS can provide valuable complement to SNP-based studies. The comparison of genomic predictive performance for total length trait under different marker selection strategies and genomic selection models indicated that GWAS selection strategy exhibits more stable predictive performance compared to the evenly selection strategy. Additionally, support vector machine model demonstrated better predictive accuracy and efficiency than traditional best linear unbiased prediction and Bayes models. Furthermore, the superior predictive performance using InDel markers compared to SNP markers highlighted the potential of InDels to enhance genomic predictive accuracy and efficiency. Our results carry significant implications for dissecting genetic mechanisms and contributing genetic improvement of growth traits in spotted sea bass through genomic resources.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 4","pages":"400-416"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.87","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142664505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The case for bovine pangenome","authors":"Wai Yee Low","doi":"10.1002/aro2.86","DOIUrl":"https://doi.org/10.1002/aro2.86","url":null,"abstract":"&lt;p&gt;The single reference genome assembly approach has been shown to be insufficient for capturing the full spectrum of genetic variation. This inadequacy has been well-documented in human genomics [&lt;span&gt;1&lt;/span&gt;] and the solution is to create a pangenome reference. A pangenome reference is a comprehensive genomic representation that captures the full genetic diversity within a species by incorporating multiple individual genomes. In agricultural genomics, the creation of a bovine pangenome is important for designing or selecting animal genomes that are better adapted to climate change, capable of reducing methane emissions, and conducive to producing healthy food for a growing global population. The Bovine Pangenome Consortium (BPC) [&lt;span&gt;2&lt;/span&gt;], which has over 60 members spread across 20 countries, has been established to coordinate global efforts in this area. At present, the BPC has collected more than 100 long-read-based genome assemblies representing ∼60 unique breeds/species. The primary goal is to construct a pangenome to enable accurate detection of genetic variation, which includes single nucleotide polymorphisms (SNPs) and structural variants (SVs) in bovine species especially cattle.&lt;/p&gt;&lt;p&gt;The BPC uses collaborative open science model and requires samples and expertise from multiple laboratories worldwide. The project focuses on global cattle breeds, including both taurine and indicine subspecies. Beyond cattle, the BPC aims to include other members of the Bovini tribe, such as water buffalo, yak, and bison, in the pangenome. In the case of water buffalo, there is a plan for a pangenome specific for the species as part of the 1000 Buffalo Genomes Project [&lt;span&gt;17&lt;/span&gt;]. The inclusion of bovine species other than cattle will facilitate comparative genomic analysis and enhance the understanding of evolutionary processes and potential introgression events [&lt;span&gt;3&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;Current genetic variant detection tools are highly sensitive to the quality and representation of reference genomes, often resulting in reference bias [&lt;span&gt;4&lt;/span&gt;]. Identification of SVs and copy number variants is sensitive to the specific reference genome chosen [&lt;span&gt;5&lt;/span&gt;]. Detection of epigenetic markers such as DNA methylation is also sensitive to the choice of reference genome [&lt;span&gt;6&lt;/span&gt;]. It is expected that in highly polymorphic and repetitive sequences, such as the major histocompatibility complex region [&lt;span&gt;7&lt;/span&gt;], a single linear reference is problematic to represent the genetic variants at this locus. These issues are some of the reasons why the BPC was formed to create bovine pangenome to improve the accuracy of genetic analyses.&lt;/p&gt;&lt;p&gt;Building pangenome graphs can be computationally challenging, especially when the number of genomes being included is high (e.g., &gt;100), and hence determining the best way to construct these references is crucial. There are at least three main methods to build a pangenome: reference-guided ","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 4","pages":"363-365"},"PeriodicalIF":0.0,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.86","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The assisted reproductive technology of in vitro embryo production","authors":"Pat Lonergan","doi":"10.1002/aro2.81","DOIUrl":"https://doi.org/10.1002/aro2.81","url":null,"abstract":"&lt;p&gt;Reproductive efficiency is the cornerstone of all animal-based agricultural enterprises and is crucial for profitable, environmentally sustainable food systems. In livestock production systems, particularly cattle production, reproductive efficiency is the main driver of farm profitability. Pregnancy loss, rather than fertilization failure, is one of the major causes of reproductive failure in cattle [&lt;span&gt;1, 2&lt;/span&gt;] and leads to extended calving intervals which, especially in a seasonal system, can have a major impact on profitability due to costs associated with increased calving intervals, increased culling, increased labor costs, and increased interventions of one form or another [&lt;span&gt;3&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;In cattle, most pregnancy failure occurs quite early after fertilization; ∼75% of conceptus loss occurs in the first 2–3 weeks of gestation, before maternal recognition of pregnancy (around day 16–17) and the start of placentation (around day 20) [&lt;span&gt;2, 4-7&lt;/span&gt;]. Indeed, in some situations (e.g., metabolic stress associated with high milk production), as many as 50% of embryos may be lost in the first week after fertilization [&lt;span&gt;4, 6&lt;/span&gt;]. Even when all of the biological and technical causes for pregnancy failure in the first week are avoided by transferring an embryo directly into the uterus (typically done on day 7 of the cycle), pregnancy success is not consistently improved compared to artificial insemination (AI) [&lt;span&gt;8&lt;/span&gt;]. Thus, improving our understanding of the underlying physiological and molecular regulation of early embryo development leading to a successful pregnancy will significantly contribute to social and economic sustainability in agri-food production, a crucial objective in the face of an ever-increasing global population [&lt;span&gt;9&lt;/span&gt;] and growing concerns about the impact of inefficient agricultural practices on the environment [&lt;span&gt;10&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;The development of AI in the 1950s has driven genetic improvement in dairy cattle and is now the main method of impregnating dairy females with semen from elite bulls [&lt;span&gt;11&lt;/span&gt;]. Since then, considerable progress has been made in the development and application of a wide range of assisted reproductive technologies (ARTs) at farm level [&lt;span&gt;12&lt;/span&gt;], including multiple ovulation embryo transfer (MOET, or ‘superovulation’), involving the generation of multiple embryos within the female (in vivo) [&lt;span&gt;13&lt;/span&gt;], ovum pick-up/in vitro fertilization (in vitro embryo production), involving the generation of embryos in the laboratory [&lt;span&gt;14, 15&lt;/span&gt;], and the use of sex-sorted semen to predetermine the offspring sex [&lt;span&gt;16-18&lt;/span&gt;]. All these technologies facilitate accelerated genetic improvement and increase the economic value of the offspring generated [&lt;span&gt;19&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;In vitro embryo production (IVP) is now an established technology in the toolbox of ARTs available to farmers and breeding companies for genetic improvem","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 4","pages":"356-359"},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.81","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obesity and oxidative stress: Implications for female fertility","authors":"Nuo Heng,&nbsp;Huabin Zhu,&nbsp;Anup Kumar Talukder,&nbsp;Shanjiang Zhao","doi":"10.1002/aro2.82","DOIUrl":"https://doi.org/10.1002/aro2.82","url":null,"abstract":"<p>Obesity has reached epidemic proportions in most parts of the world, and it is estimated that 1 billion people globally are trapped in an obesity pandemic, which has seriously compromised human health. Recently, there has been a flood of research into obesity as well as redox and lipid metabolism; however, our understanding of the pathways and specific molecular mechanisms by which obesity-induced oxidative stress affects female reproductive function remains limited. In this review, we discuss how the obesity pandemic has led to lower female fertility. We focus on multiple facets of obesity-mediated reproductive dysfunction, including follicular atresia, oocyte maturation, embryo implantation, reproductive aging, and discuss therapeutic interventions that have the potential to normalize reproductive function in obese females, such as targeting mitochondrial lipid metabolism and antioxidant pathways.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 4","pages":"377-399"},"PeriodicalIF":0.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.82","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142664826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CircDYRK1A regulates bovine myoblasts development by binding miR21-5p to affect KLF5 gene expression","authors":"Peng Yang,&nbsp;Xinmiao Li,&nbsp;Lei Du,&nbsp;Shijie Lyu,&nbsp;Zijing Zhang,&nbsp;Fengpeng Lin,&nbsp;Xinglei Qi,&nbsp;Xian Liu,&nbsp;Eryao Wang,&nbsp;Chuzhao Lei,&nbsp;Yongzhen Huang","doi":"10.1002/aro2.76","DOIUrl":"https://doi.org/10.1002/aro2.76","url":null,"abstract":"<p>Circular RNA (circRNA), a stable ring-shaped RNA molecule found in eukaryotic cells, plays significant roles in biological regulation, particularly by interfering with transcription factor binding or enhancing gene expression. Using transcriptomic sequencing, we identified differentially expressed circRNAs in bovine muscle at various time points. Specifically, circDYRK1A was discovered and shown to enhance differentiation while suppressing proliferation of adult myoblasts. Rescue experiments further demonstrated that circDYRK1A regulates the KLF5 gene expression by interacting with miR21-5p, thus exerting its influence at the transcriptional level. This study marks the first identification of circDYRK1A in cattle and elucidates its role in bovine myoblast development through the circDYRK1A-miR21-5p-KLF5 regulatory axis. These findings contribute novel insights into molecular breeding of cattle and advance fundamental research on beef cattle breeding and muscle development.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 4","pages":"431-445"},"PeriodicalIF":0.0,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.76","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature and humidity as drivers for the transmission of zoonotic diseases","authors":"Li Zhang,&nbsp;Chenrui Lv,&nbsp;Wenqiang Guo,&nbsp;Zhenzhuo Li","doi":"10.1002/aro2.75","DOIUrl":"https://doi.org/10.1002/aro2.75","url":null,"abstract":"<p>Zoonotic diseases remain a persistent threat to global public health. Many major zoonotic pathogens exhibit seasonal patterns associated with climatic variations. Quantifying the impacts of environmental variables such as temperature and humidity on disease transmission dynamics is critical for improving prediction and control measures. This review synthesizes current evidence on the relationships between temperature and humidity and major zoonotic diseases, including malaria, dengue, rabies, anisakiasis, and influenza. Overall, this review highlighted some overarching themes across the different zoonotic diseases examined. Higher temperatures within suitable ranges were generally associated with increased transmission risks, while excessively high or low temperatures had adverse effects. Humidity exhibited complex nonlinear relationships, facilitating transmission in certain temperature zones but inhibiting it in others. Heavy rainfall and high humidity were linked to vector-borne disease outbreaks such as malaria by enabling vector breeding. However, reduced incidence of some diseases like dengue fever was observed with high rainfall. To address existing knowledge gaps, future research efforts should prioritize several key areas: enhancing data quality through robust surveillance and the integration of high-resolution microclimate data; standardizing analytical frameworks and leveraging advanced methodologies such as machine learning; conducting mechanistic studies to elucidate pathogen, vector, and host responses to climatic stimuli; adopting interdisciplinary approaches to account for interacting drivers; and projecting disease impacts under various climate change scenarios to inform adaptation strategies. Investing in these research priorities can propel the development of evidence-based climate-aware disease prediction and control measures, ultimately safeguarding public health more effectively.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 3","pages":"323-336"},"PeriodicalIF":0.0,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.75","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142013489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Free iron accumulation and oxidative stress burden induce ferroptotic atrophy of chicken yolk sac during the late embryogenesis","authors":"Huichao Liu,&nbsp;Zehe Song,&nbsp;Xi He,&nbsp;Haihan Zhang","doi":"10.1002/aro2.74","DOIUrl":"10.1002/aro2.74","url":null,"abstract":"<p>The aim of this study was to investigate the mechanism of iron homeostasis and the ferroptosis pathway for yolk sac atrophy during late embryogenesis. To study the mechanism of yolk sac atrophy, 100 eggs were used. Further, 500 eggs were randomly divided into five treatments and in ovo feeding with different iron sources, such as FeSO₄, ferrous glycinate (Fe-Gly), or deferoxamine (DFO), to study the effects of free iron content on hatching quality and embryonic development. The results showed that total iron content of yolk decreased, but yolk sac increased from embryonic(E)13 to E19 (<i>p</i> &lt; 0.05). Comparison of gene expression of iron transport systems showed that free iron accumulation and dysfunction occurred in the yolk sac. Yolk sac metabolites at E19 compared to E13 were more enriched in histidine and sulfur pathways, suppressing glutathione synthesis and resulting in oxidative stress damage in the yolk sac. Combined analysis of differential metabolites and gene expression in ferroptosis pathway at E13 and E19 revealed the activation of the yolk sac during late embryogenesis was probably through up-regulation of <i>ACSL4</i> expression and down-regulation of <i>GPX4</i> expression. Furthermore, in ovo feeding FeSO₄ shortened the incubation time compared to CON, while Fe-Gly or DFO delayed the hatching peak and increased hatching weight with less residual yolk. Collectively, it can be concluded that yolk sac atrophy during late embryogenesis may be mediated by iron disorders and provides a novel insight to modulate yolk sac nutrition, and hatching efficiency in chickens.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"2 3","pages":"285-299"},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.74","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141668547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}