Animal Research and One Health最新文献

{"title":"Modulating Taste Perception in Newly Weaned Piglets: The Role of Palatability Additives in Enhancing Feed Intake and Performance","authors":"Luana Specht,&nbsp;Gabriela Altenhofen,&nbsp;Daiane Carvalho,&nbsp;Michele Fangmeier","doi":"10.1002/aro2.70039","DOIUrl":"https://doi.org/10.1002/aro2.70039","url":null,"abstract":"<p>The continuous growth of global agribusiness stimulates the search for alternatives that can enhance the performance and quality of life of production animals. In swine production systems, multiple challenges arise during the post-weaning phase when piglets face substantial transitions, including diet changes, environmental shifts, and maternal separation. These stressors often lead to reduced feed intake and inadequate nutrient consumption, resulting in impaired growth and increased susceptibility to diseases, even mortality in extreme cases. To counter these challenges, the use of palatability enhancers has emerged as an essential strategy to stimulate feed consumption, ensuring sufficient nutrient intake and promoting the health and welfare of piglets. Considering the crucial role of taste perception and dietary preferences in pigs, this review explores the mechanisms behind taste perception, the impact of various palatability additives on feed intake, and highlights current advances in strategies designed to optimize the feeding of newly weaned piglets.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"4 3","pages":"240-246"},"PeriodicalIF":0.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.70039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Lycium barbarum Residue Substituting Dietary Maize on Fecal Microbiota and Growth Performance of Crossbred Simmental Cattle","authors":"Kun Cai,&nbsp;Mingtan Li,&nbsp;Lihang He,&nbsp;Ning Ma,&nbsp;Haoyu Liu,&nbsp;Chi Xu,&nbsp;Xianhua Wu,&nbsp;Zeguang Xing,&nbsp;Qian Xing,&nbsp;Yuankai Qu,&nbsp;Xi Ma","doi":"10.1002/aro2.70033","DOIUrl":"https://doi.org/10.1002/aro2.70033","url":null,"abstract":"<p>Because of the global shortage of maize resources, the identification of environmentally sustainable and economically viable maize substitutes is imperative for the sustainable intensification of the livestock sector. This study evaluated the effects of dietary replacement of maize with <i>Lycium barbarum</i> residue (LBR) on the growth performance and fecal microbiota of crossbred Simmental cattle. A total of 20 male crossbred Simmental cattle (6 months old, 601.90 ± 34.33 kg) were randomly divided into 2 groups: control group (CON), fed basic diet; LBR group, fed 1.80% LBR replacing maize for 45 days. Results showed that growth performance and anti-inflammatory capacity were significantly enhanced in the LBR group (<i>p</i> &lt; 0.05). In addition, LBR maintained serum redox homeostasis. Through 16S rRNA genome sequencing, LBR significantly increased the abundance of <i>Rikenellaceae_RC9_gut_group</i> and <i>Oscillospiraceae_UCG_005</i> in feces (<i>p</i> &lt; 0.05). Moreover, <i>Rikenellaceae_RC9_gut_group</i> exhibited positive correlation with average daily gain (<i>p</i> &lt; 0.05). PICRUSt functional prediction analysis showed that LBR significantly changed the fatty acid metabolism and carbohydrate metabolism pathways. In conclusion, LBR as a dietary maize substitute can effectively improve the growth performance of crossbred Simmental cattle, which may be due to its regulating anti-inflammatory ability, redox homeostasis, and fecal microbiota.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"4 3","pages":"335-346"},"PeriodicalIF":0.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.70033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a Product Intended for Microbiological Control in Animal Production, and Its Combination With Halquinol","authors":"Alberto Gonçalves Evangelista,&nbsp;Lucas dos Santos Janotto,&nbsp;Adriana Paula Possamai,&nbsp;Carolina Cini Perry,&nbsp;Francine Lemes Ribeiro,&nbsp;Rafael Canonenco de Araujo,&nbsp;Fernando Bittencourt Luciano","doi":"10.1002/aro2.70030","DOIUrl":"https://doi.org/10.1002/aro2.70030","url":null,"abstract":"<p>Antimicrobial resistance is an increasingly pressing global concern, with one of its contributing factors being the use of subtherapeutic doses of antibiotics in animal production for zootechnical purposes. Halquinol (HA) remains one of the few compounds still permitted in certain regions; however, the development of alternative solutions is imperative. This study evaluated the in vitro effects of a formulation composed of essential oils and organic acids against <i>Salmonella</i> and <i>Escherichia coli</i> as well as its interaction with HA. The formulation demonstrated both inhibitory and bactericidal activity against the bacteria, with effective concentrations ranging from 2.0 to 8.0 mL/L. In contrast, HA exhibited solely bacteriostatic effects, with minimum inhibitory concentrations ranging from 37.5 to 300 μg/mL. When used in combination, the compounds predominantly resulted in additive or indifferent interactions: 51.35% of the assays showed additive effects, 43.24% exhibited no interaction, and only 5.41% demonstrated antagonistic interactions. Importantly, the formulation did not induce bacterial resistance or adaptation following sublethal exposure. Conversely, sublethal exposure to HA led to an approximately tenfold increase in its bacteriostatic dose. Moreover, the formulation showed potential to mitigate resistance induced by sublethal HA exposure. In a simulated swine digestion, the combination of the formulation with HA was particularly effective against <i>E. coli</i>, with the co-administration reducing the required concentrations of the individual compounds to achieve sustained bacterial suppression throughout the digestive process. Based on these findings, the tested formulation demonstrates promising potential for bacterial control and may be used in combination with HA to reduce its required dosage.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"4 3","pages":"302-313"},"PeriodicalIF":0.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Functions of Dietary Rubber Seed Oil Supplementation: Enriching N-3 Polyunsaturated Fatty Acids and Enhancing Antioxidant Capacity in Pekin Ducks","authors":"Lei Zhuang,&nbsp;Yongbao Wu,&nbsp;Zitao Zhao,&nbsp;Junting Cao,&nbsp;Yanhong Guo,&nbsp;Zhanyue Wu,&nbsp;Qimeng Wang,&nbsp;Peilong Yang,&nbsp;Sen Wu,&nbsp;Zhiguo Wen,&nbsp;Shuisheng Hou","doi":"10.1002/aro2.70020","DOIUrl":"https://doi.org/10.1002/aro2.70020","url":null,"abstract":"<p>The present study aimed to determine whether rubber seed oil (RSO) could simultaneously increase antioxidant capacity and enrich n-3 PUFAs in Pekin ducks by evaluating growth performance, fatty acid profile, and antioxidant capacity. A total of 192 male Pekin ducks (1 day old, 51.9 ± 0.3 g) were randomly allocated to four treatment groups, each consisting of four replicates with 12 ducklings per replicate. Ducks were fed with experimental diets supplemented with 0 (Control), 1% (RSO1), 2% (RSO2), and 3% (RSO3) RSO, respectively, for 42 days. Dietary RSO supplementation improved weight gain (<i>p</i> &lt; 0.05), decreased feed/gain (<i>p</i> &lt; 0.05), and reduced the yield of abdominal fat and liver (<i>p</i> &lt; 0.05) of Pekin ducks. Total cholesterol and triglyceride contents were decreased (<i>p</i> &lt; 0.05), whereas the contents of n-3 PUFAs in plasma, liver, and breast meat increased in Pekin ducks with increasing dietary RSO levels (<i>p</i> &lt; 0.05). Simultaneously, the antioxidant activities in plasma, liver, and breast meat were enhanced in Pekin ducks after feeding RSO diets (<i>p</i> &lt; 0.05). Additionally, dietary RSO increased the mRNA expression levels related to cholesterol transport (<i>p</i> &lt; 0.05) and decreased the mRNA expression levels related to lipid synthesis in the liver of Pekin ducks (<i>p</i> &lt; 0.05). In summary, dietary RSO supplementation improved growth performance, enriched n-3 PUFAs, and enhanced antioxidant capacity in Pekin ducks. These findings suggest that RSO could serve as a novel source of n-3 PUFAs and an antioxidant for Pekin ducks, with the potential to contribute to the generation of animal functional foods.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"4 3","pages":"247-260"},"PeriodicalIF":0.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.70020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulating Taste Perception in Newly Weaned Piglets: The Role of Palatability Additives in Enhancing Feed Intake and Performance","authors":"Luana Specht,&nbsp;Gabriela Altenhofen,&nbsp;Daiane Carvalho,&nbsp;Michele Fangmeier","doi":"10.1002/aro2.70039","DOIUrl":"https://doi.org/10.1002/aro2.70039","url":null,"abstract":"<p>The continuous growth of global agribusiness stimulates the search for alternatives that can enhance the performance and quality of life of production animals. In swine production systems, multiple challenges arise during the post-weaning phase when piglets face substantial transitions, including diet changes, environmental shifts, and maternal separation. These stressors often lead to reduced feed intake and inadequate nutrient consumption, resulting in impaired growth and increased susceptibility to diseases, even mortality in extreme cases. To counter these challenges, the use of palatability enhancers has emerged as an essential strategy to stimulate feed consumption, ensuring sufficient nutrient intake and promoting the health and welfare of piglets. Considering the crucial role of taste perception and dietary preferences in pigs, this review explores the mechanisms behind taste perception, the impact of various palatability additives on feed intake, and highlights current advances in strategies designed to optimize the feeding of newly weaned piglets.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"4 3","pages":"240-246"},"PeriodicalIF":0.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.70039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Baicalin Supplementation Can Enhance the Growth Performance of Weaned Piglets and Maintain the Intestinal Barrier Integrity","authors":"Yuhui Gao,&nbsp;Jie Liu,&nbsp;Rui Yang,&nbsp;Xizhen Fan,&nbsp;Cheng Liang,&nbsp;Xin'e Shi,&nbsp;Jianjun Jin","doi":"10.1002/aro2.70023","DOIUrl":"https://doi.org/10.1002/aro2.70023","url":null,"abstract":"<p>This study aimed to elucidate the promoting effect of baicalin (BAI) on the growth performance of nonpathological piglets and its potential mechanism of improving intestinal barrier function. In this experiment, 240 21-day-old weaned piglets were randomly divided into four treatment groups, consisting of a control group (CON) and groups supplemented with 0.05%, 0.10%, and 0.15% BAI. Compared with the CON group, BAI significantly decreased the ratio of average daily feed intake to average daily gain (F/G ratio) on days 0–7 and 7–14 (<i>p</i> &lt; 0.05) and reduced the diarrhea rate in piglets. Additionally, the 0.10% and 0.15% BAI groups had significantly higher villus height (VH; <i>p</i> &lt; 0.01), and the 0.10% BAI group had a significantly higher Villus height to crypt depth ratio (VCR; <i>p</i> &lt; 0.05), indicating an enhanced intestinal absorption capacity. BAI supplementation significantly increased the expression of intestinal tight junction proteins and antioxidant enzymes, the number of goblet cells, and mucin2 (MUC2) expression (<i>p</i> &lt; 0.05); reduced the expression of pro-inflammatory factors; and improved the intestinal microbiota structure. Specifically, adding 0.10% BAI significantly increased the abundance of beneficial microorganisms such as <i>Eubacterium</i> and <i>Blautia</i> (<i>p</i> &lt; 0.05), while reducing the composition of harmful microorganisms such as <i>Proteobacteria</i> and <i>Spirochetes</i>. Metabolomic results revealed that BAI supplementation significantly increased the concentrations of beneficial metabolites for gut health, such as <i>taurine</i> and <i>rosmarinic acid</i> (<i>p</i> &lt; 0.05). In conclusion, this experiment elucidated the potential ability and intrinsic mechanism of BAI in protecting the intestinal health of piglets and ameliorating the problem of weaning stress.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"4 3","pages":"261-277"},"PeriodicalIF":0.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.70023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification Epitope on VP4 Protein of Senecavirus A Recognized by a Monoclonal Antibody","authors":"Liang Meng,&nbsp;Xuyan Xiang,&nbsp;Xinyu Qi,&nbsp;Hanrong Zhou,&nbsp;Peiyu Xiao,&nbsp;Fandan Meng,&nbsp;Tongqing An,&nbsp;Haiwei Wang","doi":"10.1002/aro2.70029","DOIUrl":"https://doi.org/10.1002/aro2.70029","url":null,"abstract":"<p>Senecavirus A (SVA) is a causative agent of vesicular disease in pigs and has gained global attention in recent years. SVA infection leads to vesicular lesions and hoof deformities in pigs, posing a significant threat to the swine industry. The VP4 protein is a crucial component of the SVA capsid, located on the inner surface of the virus particle, and plays a key role in the viral life cycle. However, its exact function and immunological characteristics remain poorly understood. Additionally, the VP4 protein of picornaviruses is known to form antigenic epitopes during viral infection, providing potential targets for antibody development and vaccine design. Nevertheless, due to the unique location of VP4, these epitopes remain largely unexplored. In this study, a monoclonal antibody (mAb) 4E4 was successfully generated by immunizing mice with purified SVA VP4 protein. The mAb 4E4 specifically recognizes recombinant VP4 protein and the SVA-infected cells. Further epitope mapping using truncated VP4 proteins detected with Western blotting defined a key B-cell epitope, ⁷SKDNFD¹², which is exposed on the surface of VP4 protein and is highly conserved among different SVA strains. Additionally, mAb 4E4 showed neutralizing activity against SVA. This study provides the first identification of a conserved antigenic epitope on the SVA VP4 protein. This finding provides valuable tools for further investigation of VP4 protein function and lays the foundation for developing novel diagnostic and vaccine strategies.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"4 3","pages":"293-301"},"PeriodicalIF":0.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of Interspecies Somatic Cell Nuclear Transfer and Transgene-Free Inducible Pluripotent Stem Cells for Versatile Conservation of the Germplasm Resource of Wild Boar","authors":"Chen Gao,&nbsp;Xinyi Zhou,&nbsp;Shigang Gu,&nbsp;Jianbo Li,&nbsp;Jing Wang,&nbsp;Yurong Zhang,&nbsp;Wenxuan Zhao,&nbsp;Xinhua Wei,&nbsp;Liting Lu,&nbsp;Quanmin Zhao,&nbsp;Yongye Huang,&nbsp;Dawei Yu","doi":"10.1002/aro2.70025","DOIUrl":"https://doi.org/10.1002/aro2.70025","url":null,"abstract":"<p>Cloning via somatic cell nuclear transfer and establishing inducible pluripotent stem cells is critical for the conservation of the cherished germplasm resource. The wild boar population would be altered due to human activities and climate change. Recently, we have successfully generated cloned wild boars via interspecies somatic cell nuclear transfer using wild boar fibroblast cells as donor cells, domestic porcine oocytes as recipients, and Bama pigs as surrogate sows. We have also harvested wild boar inducible pluripotent stem cells free of selection genes utilizing the piggyBac transposon system. This study sheds light on preserving the genetic resource of wild boar and provides a reference for protecting other wildlife.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"4 3","pages":"347-351"},"PeriodicalIF":0.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.70025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Restricted Nursing and a Bovine Appeasing Substance on the Welfare, Growth Performance, and Response to Vaccination of Beef Calves at Weaning","authors":"Denise E. Mussalafo,&nbsp;Marcelo Vedovatto,&nbsp;Aylpy R. D. Santos,&nbsp;Dalton M. Oliveira,&nbsp;Fabiana A. M. Sterza,&nbsp;Mariana Santos,&nbsp;Helena B. C. R. Batista,&nbsp;Jaíne G. Garcia,&nbsp;Andréa R. D. L. Souza","doi":"10.1002/aro2.70031","DOIUrl":"https://doi.org/10.1002/aro2.70031","url":null,"abstract":"<p>This study evaluated the effects of restricted nursing (RN) and the administration of a bovine appeasing substance (BAS) given 8 days before (d-8) and on the day of weaning (d0) as a strategy to mitigate stress and optimize the post-weaning growth performance of beef calves, with emphasis on behavior and response to vaccination. Forty male Nelore calves were randomly assigned to four treatments with ten replicates: control (CON)—topical administration of 5 mL of saline solution on d0; BAS-8—topical administration of 5 mL of BAS on d-8; BAS0—topical administration of 5 mL of BAS on d0; and RN—calves allowed to nurse only one hour/day from d-8 to d0. Calves in the CON treatment group showed lower average daily gain compared to the other treatments (<i>p</i> &lt; 0.05). There was a trend (<i>p</i> = 0.09) for BAS-8 and BAS0 to result in lower temperament scores when calves entered the restraint chute. BAS0 calves had greater (<i>p</i> = 0.05) serum titer concentrations against rabies compared to the other treatments. The RN and BAS administration eight days before and on the day of weaning was effective in improving growth performance in beef calves.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"4 3","pages":"314-321"},"PeriodicalIF":0.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.70031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Restricted Nursing and a Bovine Appeasing Substance on the Welfare, Growth Performance, and Response to Vaccination of Beef Calves at Weaning","authors":"Denise E. Mussalafo,&nbsp;Marcelo Vedovatto,&nbsp;Aylpy R. D. Santos,&nbsp;Dalton M. Oliveira,&nbsp;Fabiana A. M. Sterza,&nbsp;Mariana Santos,&nbsp;Helena B. C. R. Batista,&nbsp;Jaíne G. Garcia,&nbsp;Andréa R. D. L. Souza","doi":"10.1002/aro2.70031","DOIUrl":"https://doi.org/10.1002/aro2.70031","url":null,"abstract":"<p>This study evaluated the effects of restricted nursing (RN) and the administration of a bovine appeasing substance (BAS) given 8 days before (d-8) and on the day of weaning (d0) as a strategy to mitigate stress and optimize the post-weaning growth performance of beef calves, with emphasis on behavior and response to vaccination. Forty male Nelore calves were randomly assigned to four treatments with ten replicates: control (CON)—topical administration of 5 mL of saline solution on d0; BAS-8—topical administration of 5 mL of BAS on d-8; BAS0—topical administration of 5 mL of BAS on d0; and RN—calves allowed to nurse only one hour/day from d-8 to d0. Calves in the CON treatment group showed lower average daily gain compared to the other treatments (<i>p</i> &lt; 0.05). There was a trend (<i>p</i> = 0.09) for BAS-8 and BAS0 to result in lower temperament scores when calves entered the restraint chute. BAS0 calves had greater (<i>p</i> = 0.05) serum titer concentrations against rabies compared to the other treatments. The RN and BAS administration eight days before and on the day of weaning was effective in improving growth performance in beef calves.</p>","PeriodicalId":100086,"journal":{"name":"Animal Research and One Health","volume":"4 3","pages":"314-321"},"PeriodicalIF":0.0,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/aro2.70031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148662517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}